EncuA.00790.a

CYSTEINYL tRNA SYNTHETASE

CENTER ID: EncuA.00790.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00790.a.A1.GE27810 Full length( EncuA.00790.a ) 1 480
EncuA.00790.a.A1.GE28324 Full length( EncuA.00790.a ) 1 480
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00790.a.A1.PW27701 Full length( EncuA.00790.a ) 1 480

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU08_0490
RefSeq: NP_597178.1
UniProt: Q8SRE0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKTLKLYNS ITRTIDIFTP RKGNEVKMYI CGPTVYDSSH IGHARTYVMF DVIRRVLSDY LKYNVRFVMN ITDIDDKIIA RANETGASME EVTRKYTEEF FEDMKTLNVR SPSFVTFVTS YVDKIVKFIE KLEANGLAYE SRGSVYFDLN SYQQRYSYPL FKSKDGINSE GDENKDKRSP CDFVLWKRSK ENEPRYESKW GHGRPGWHIE CSVMSSDILG EDLDIHAGGV DLAFPHHENE IAQCQAYFMQ EPWVKCFLHT GHLNISGLKM SKSLKNFTTI KEALKTISPR QLRVLFLHHQ WNKDMNYEKE HLKFAETIEK KIFNFMSVAE SMRKNALAFE TLENADREVL RELGNVQEAV HAALLDNVDT PAVMKRIVEM INFTNARIKT ISPSTVLVVK DYIKEITDVL GLSEEERQES PGEDLIAQLL SNFRESIREM ARRKEPYSKF LEKCDWIRES IKDYGYIIED NSEGSILRKK
NT Sequence
ATGGACAAGA CTCTGAAGCT CTACAATTCG ATTACCAGGA CCATTGATAT CTTTACTCCT AGAAAAGGCA ACGAGGTCAA GATGTATATA TGCGGGCCAA CTGTGTACGA CTCTTCCCAC ATAGGACATG CAAGGACCTA TGTGATGTTT GATGTCATAC GGCGTGTCCT GAGCGACTAT CTGAAGTATA ATGTGAGATT TGTGATGAAC ATAACAGACA TCGATGACAA GATAATAGCC AGAGCCAATG AGACTGGGGC ATCCATGGAA GAAGTTACCA GGAAATACAC TGAAGAGTTT TTTGAGGACA TGAAGACCTT GAATGTCAGG AGCCCCTCGT TTGTGACGTT TGTGACGTCG TATGTCGACA AGATTGTGAA GTTTATTGAA AAGCTTGAGG CAAACGGGCT TGCATACGAA AGCAGAGGGA GTGTGTACTT TGATCTGAAC AGTTATCAGC AGAGATACAG CTACCCTCTA TTCAAGAGCA AGGACGGCAT CAACTCTGAA GGAGATGAAA ACAAGGACAA AAGAAGCCCG TGCGACTTTG TCCTCTGGAA GAGGTCCAAG GAAAATGAGC CACGGTATGA GTCGAAATGG GGACATGGGA GGCCTGGATG GCATATTGAG TGCAGTGTAA TGTCCAGTGA TATTCTCGGA GAAGACCTCG ACATACATGC TGGAGGAGTC GACCTTGCAT TTCCACACCA CGAGAACGAG ATAGCTCAGT GCCAAGCCTA CTTTATGCAG GAGCCCTGGG TGAAGTGCTT TCTCCACACA GGGCATCTGA ACATCAGCGG GCTGAAGATG TCCAAGTCCC TGAAGAACTT TACAACGATT AAGGAAGCAC TGAAAACGAT TTCCCCTCGC CAGCTAAGGG TTCTGTTTCT ACACCATCAG TGGAACAAGG ACATGAACTA CGAGAAAGAG CACCTGAAGT TTGCAGAAAC CATTGAGAAG AAGATCTTCA ACTTTATGTC TGTGGCGGAG AGTATGAGAA AGAACGCACT GGCATTTGAG ACTCTAGAAA ATGCGGACAG GGAAGTTCTG AGGGAGCTAG GGAATGTCCA GGAAGCTGTG CATGCAGCAC TTCTTGACAA TGTTGACACG CCTGCTGTAA TGAAGAGGAT AGTGGAGATG ATAAATTTTA CAAATGCCAG AATAAAGACA ATCTCACCGT CTACCGTTCT GGTTGTCAAG GACTACATCA AGGAGATCAC AGACGTGCTT GGATTGAGTG AGGAGGAGAG ACAGGAATCC CCTGGAGAGG ATCTGATTGC CCAACTGCTT AGCAACTTCA GAGAAAGTAT TAGGGAGATG GCAAGACGAA AGGAGCCTTA TTCGAAGTTC CTTGAGAAGT GCGATTGGAT CCGAGAAAGC ATCAAGGACT ACGGATATAT TATCGAAGAT AACAGTGAAG GATCTATACT GAGAAAGAAA
Details for EncuA.00790.a.A1.GE27810
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDKTLKLYN SITRTIDIFT PRKGNEVKMY ICGPTVYDSS HIGHARTYVM LDVIRRVLSD YLKYNVRFVM NITDIDDKII ARANETGASM EEVTRKYTEE FFEDMKTLNV RSPSFVTFVT SYVDKIVKFI EKLEANGLAY ESRGSVYFDL NSYQQRYSYP LFKSKDGINS EGDENKGKRS PCDFVLWKRS KENEPRYESK WGHGRPGWHI ECSVMSSDIL GXDLDIHAGG VDLAFPHHEN EIAQCQAYFM QEPWVKCFLH TGHLNISGLK MSKSLKNFTT IKEALKTISP RQLRVLFLHH QWNKDMNYEK EHLKFAETIE KKIFNFMSVA ESMRKNALAF ETLENADREV LRELGNVQEA VHAALLDNVD TPAVMKRIVE MINFTNARIK TISPSTVLVV KDYIKEITDV LGLSEEERQE SPGEDLIAQL LSNFRESIRE MARRKEPYSK FLEKCDWIRE SIKDYGYIIE DNSEGSILR
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaagact ctgaagctct acaattcgat taccaggacc attgatatct ttactcctag aaaaggcaac gaggtcaaga tgtatatatg cgggccaact gtgtacgact cttcccacat aggacatgca aggacctatg tgatgcttga tgtcatacgg cgtgtcctga gcgactatct gaagtataat gtgagatttg tgatgaacat aacagacatc gatgacaaga taatagccag agccaatgag actggggcat ccatggaaga agttaccagg aaatacactg aagagttttt tgaggacatg aagaccttga atgtcaggag cccctcgttt gtgacgtttg tgacgtcgta tgtcgacaag attgtgaagt ttattgaaaa gcttgaggca aacgggcttg catacgaaag cagagggagt gtgtactttg atctgaacag ttatcagcag agatacagct accctctatt caagagcaag gacggcatca actctgaagg agatgaaaac aagggcaaaa gaagcccgtg cgactttgtc ctctggaaga ggtccaagga aaatgagcca cggtatgagt cgaaatgggg acatgggagg cctggatggc atattgagtg cagtgtaatg tccagtgata ttctcggana agacctcgac atacatgctg gaggagtcga cctngcattt ccacaccacg agaacgagat agctcagtgc caagcctact ttatgcagga gccctgggtg aagtgctttc tccacacagg gcatctgaac atcagcgggc tgaagatgtc caagtccctg aagaacttta caacgattaa ggaagcactg aaaacgattt cccctcgcca gctaagggtt ctgtttctac accatcagtg gaacaaggac atgaactacg agaaagagca cctgaagttt gcagaaacca ttgagaagaa gatcttcaac tttatgtctg tggcggagag tatgagaaag aacgcactgg catttgagac tctagaaaat gcggacaggg aagttctgag ggagctaggg aatgtccagg aagctgtgca tgcagcactt cttgacaatg ttgacacgcc tgctgtaatg aagaggatag tggagatgat aaattttaca aatgccagaa taaagacaat ctcaccgtct accgttctgg ttgtcaagga ctacatcaag gagatcacag acgtgcttgg attgagtgag gaggagagac aggaatcccc tggagaggat ctgattgccc aactgcttag caacttcaga gaaagtatta gggagatggc aagacgaaag gagccttatt cgaagttcct tgagaagtgc gattggatcc gagaaagcat caaggactac ggatatatta tcgaagataa cagtgaagga tctatactga gaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDKTLKLYN SITRTIDIFT PRKGNEVKMY ICGPTVYDSS HIGHARTYVM FDVIRRVLSD YLKYNVRFVM NITDIDDKII ARANETGASM EEVTRKYTEE FFEDMKTLNV RSPSFVTFVT SYVDKIVKFI EKLEANGLAY ESRGSVYFDL NSYQQRYSYP LFKSKDGINS EGDENKDKRS PCDFVLWKRS KENEPRYESK WGHGRPGWHI ECSVMSSDIL GEDLDIHAGG VDLAFPHHEN EIAQCQAYFM QEPWVKCFLH TGHLNISGLK MSKSLKNFTT IKEALKTISP RQLRVLFLHH QWNKDMNYEK EHLKFAETIE KKIFNFMSVA ESMRKNALAF ETLENADREV LRELGNVQEA VHAALLDNVD TPAVMKRIVE MINFTNARIK TISPSTVLVV KDYIKEITDV LGLSEEERQE SPGEDLIAQL LSNFRESIRE MARRKEPYSK FLEKCDWIRE SIKDYGYIIE DNSEGSILRK K
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaagact ctgaagctct acaattcgat taccaggacc attgatatct ttactcctag aaaaggcaac gaggtcaaga tgtatatatg cgggccaact gtgtacgact cttcccacat aggacatgca aggacctatg tgatgtttga tgtcatacgg cgtgtcctga gcgactatct gaagtataat gtgagatttg tgatgaacat aacagacatc gatgacaaga taatagccag agccaatgag actggggcat ccatggaaga agttaccagg aaatacactg aagagttttt tgaggacatg aagaccttga atgtcaggag cccctcgttt gtgacgtttg tgacgtcgta tgtcgacaag attgtgaagt ttattgaaaa gcttgaggca aacgggcttg catacgaaag cagagggagt gtgtactttg atctgaacag ttatcagcag agatacagct accctctatt caagagcaag gacggcatca actctgaagg agatgaaaac aaggacaaaa gaagcccgtg cgactttgtc ctctggaaga ggtccaagga aaatgagcca cggtatgagt cgaaatgggg acatgggagg cctggatggc atattgagtg cagtgtaatg tccagtgata ttctcggaga agacctcgac atacatgctg gaggagtcga ccttgcattt ccacaccacg agaacgagat agctcagtgc caagcctact ttatgcagga gccctgggtg aagtgctttc tccacacagg gcatctgaac atcagcgggc tgaagatgtc caagtccctg aagaacttta caacgattaa ggaagcactg aaaacgattt cccctcgcca gctaagggtt ctgtttctac accatcagtg gaacaaggac atgaactacg agaaagagca cctgaagttt gcagaaacca ttgagaagaa gatcttcaac tttatgtctg tggcggagag tatgagaaag aacgcactgg catttgagac tctagaaaat gcggacaggg aagttctgag ggagctaggg aatgtccagg aagctgtgca tgcagcactt cttgacaatg ttgacacgcc tgctgtaatg aagaggatag tggagatgat aaattttaca aatgccagaa taaagacaat ctcaccgtct accgttctgg ttgtcaagga ctacatcaag gagatcacag acgtgcttgg attgagtgag gaggagagac aggaatcccc tggagaggat ctgattgccc aactgcttag caacttcaga gaaagtatta gggagatggc aagacgaaag gagccttatt cgaagttcct tgagaagtgc gattggatcc gagaaagcat caaggactac ggatatatta tcgaagataa cagtgaagga tctatactga gaaagaaaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for EncuA.00790.a.A1.GE28324
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDKTLKLYN SITRTIDIFT PRKGNEVKMY ICGPTVYDSS HIGHARTYVM LDVIRRVLSD YLKYNVRFVM NITDIDDKII ARANETGASM EEVTRKYTEE FFEDMKTLNV RSPSFVTFVT SYVDKIVKFI EKLEANGLAY ESRGSVYFDL NSYQQRYSYP LFKSKDGINS EGDENKGKRS PCDFVLWKRS KENEPRYESK WGHGRPGWHI ECSVMSSDIL GXDLDIHAGG VDLAFPHHEN EIAQCQAYFM QEPWVKCFLH TGHLNISGLK MSKSLKNFTT IKEALKTISP RQLRVLFLHH QWNKDMNYEK EHLKFAETIE KKIFNFMSVA ESMRKNALAF ETLENADREV LRELGNVQEA VHAALLDNVD TPAVMKRIVE MINFTNARIK TISPSTVLVV KDYIKEITDV LGLSEEERQE SPGEDLIAQL LSNFRESIRE MARRKEPYSK FLEKCDWIRE SIKDYGYIIE DNSEGSILR
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaagact ctgaagctct acaattcgat taccaggacc attgatatct ttactcctag aaaaggcaac gaggtcaaga tgtatatatg cgggccaact gtgtacgact cttcccacat aggacatgca aggacctatg tgatgcttga tgtcatacgg cgtgtcctga gcgactatct gaagtataat gtgagatttg tgatgaacat aacagacatc gatgacaaga taatagccag agccaatgag actggggcat ccatggaaga agttaccagg aaatacactg aagagttttt tgaggacatg aagaccttga atgtcaggag cccctcgttt gtgacgtttg tgacgtcgta tgtcgacaag attgtgaagt ttattgaaaa gcttgaggca aacgggcttg catacgaaag cagagggagt gtgtactttg atctgaacag ttatcagcag agatacagct accctctatt caagagcaag gacggcatca actctgaagg agatgaaaac aagggcaaaa gaagcccgtg cgactttgtc ctctggaaga ggtccaagga aaatgagcca cggtatgagt cgaaatgggg acatgggagg cctggatggc atattgagtg cagtgtaatg tccagtgata ttctcggana agacctcgac atacatgctg gaggagtcga cctngcattt ccacaccacg agaacgagat agctcagtgc caagcctact ttatgcagga gccctgggtg aagtgctttc tccacacagg gcatctgaac atcagcgggc tgaagatgtc caagtccctg aagaacttta caacgattaa ggaagcactg aaaacgattt cccctcgcca gctaagggtt ctgtttctac accatcagtg gaacaaggac atgaactacg agaaagagca cctgaagttt gcagaaacca ttgagaagaa gatcttcaac tttatgtctg tggcggagag tatgagaaag aacgcactgg catttgagac tctagaaaat gcggacaggg aagttctgag ggagctaggg aatgtccagg aagctgtgca tgcagcactt cttgacaatg ttgacacgcc tgctgtaatg aagaggatag tggagatgat aaattttaca aatgccagaa taaagacaat ctcaccgtct accgttctgg ttgtcaagga ctacatcaag gagatcacag acgtgcttgg attgagtgag gaggagagac aggaatcccc tggagaggat ctgattgccc aactgcttag caacttcaga gaaagtatta gggagatggc aagacgaaag gagccttatt cgaagttcct tgagaagtgc gattggatcc gagaaagcat caaggactac ggatatatta tcgaagataa cagtgaagga tctatactga gaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDKTLKLYN SITRTIDIFT PRKGNEVKMY ICGPTVYDSS HIGHARTYVM FDVIRRVLSD YLKYNVRFVM NITDIDDKII ARANETGASM EEVTRKYTEE FFEDMKTLNV RSPSFVTFVT SYVDKIVKFI EKLEANGLAY ESRGSVYFDL NSYQQRYSYP LFKSKDGINS EGDENKDKRS PCDFVLWKRS KENEPRYESK WGHGRPGWHI ECSVMSSDIL GEDLDIHAGG VDLAFPHHEN EIAQCQAYFM QEPWVKCFLH TGHLNISGLK MSKSLKNFTT IKEALKTISP RQLRVLFLHH QWNKDMNYEK EHLKFAETIE KKIFNFMSVA ESMRKNALAF ETLENADREV LRELGNVQEA VHAALLDNVD TPAVMKRIVE MINFTNARIK TISPSTVLVV KDYIKEITDV LGLSEEERQE SPGEDLIAQL LSNFRESIRE MARRKEPYSK FLEKCDWIRE SIKDYGYIIE DNSEGSILRK K
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaagact ctgaagctct acaattcgat taccaggacc attgatatct ttactcctag aaaaggcaac gaggtcaaga tgtatatatg cgggccaact gtgtacgact cttcccacat aggacatgca aggacctatg tgatgtttga tgtcatacgg cgtgtcctga gcgactatct gaagtataat gtgagatttg tgatgaacat aacagacatc gatgacaaga taatagccag agccaatgag actggggcat ccatggaaga agttaccagg aaatacactg aagagttttt tgaggacatg aagaccttga atgtcaggag cccctcgttt gtgacgtttg tgacgtcgta tgtcgacaag attgtgaagt ttattgaaaa gcttgaggca aacgggcttg catacgaaag cagagggagt gtgtactttg atctgaacag ttatcagcag agatacagct accctctatt caagagcaag gacggcatca actctgaagg agatgaaaac aaggacaaaa gaagcccgtg cgactttgtc ctctggaaga ggtccaagga aaatgagcca cggtatgagt cgaaatgggg acatgggagg cctggatggc atattgagtg cagtgtaatg tccagtgata ttctcggaga agacctcgac atacatgctg gaggagtcga ccttgcattt ccacaccacg agaacgagat agctcagtgc caagcctact ttatgcagga gccctgggtg aagtgctttc tccacacagg gcatctgaac atcagcgggc tgaagatgtc caagtccctg aagaacttta caacgattaa ggaagcactg aaaacgattt cccctcgcca gctaagggtt ctgtttctac accatcagtg gaacaaggac atgaactacg agaaagagca cctgaagttt gcagaaacca ttgagaagaa gatcttcaac tttatgtctg tggcggagag tatgagaaag aacgcactgg catttgagac tctagaaaat gcggacaggg aagttctgag ggagctaggg aatgtccagg aagctgtgca tgcagcactt cttgacaatg ttgacacgcc tgctgtaatg aagaggatag tggagatgat aaattttaca aatgccagaa taaagacaat ctcaccgtct accgttctgg ttgtcaagga ctacatcaag gagatcacag acgtgcttgg attgagtgag gaggagagac aggaatcccc tggagaggat ctgattgccc aactgcttag caacttcaga gaaagtatta gggagatggc aagacgaaag gagccttatt cgaagttcct tgagaagtgc gattggatcc gagaaagcat caaggactac ggatatatta tcgaagataa cagtgaagga tctatactga gaaagaaaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for EncuA.00790.a.A1.PW27701
PURIFICATION DATe: 11/17/2009
CONCENTRATION: 20.02mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 26
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDKTLKLYN SITRTIDIFT PRKGNEVKMY ICGPTVYDSS HIGHARTYVM LDVIRRVLSD YLKYNVRFVM NITDIDDKII ARANETGASM EEVTRKYTEE FFEDMKTLNV RSPSFVTFVT SYVDKIVKFI EKLEANGLAY ESRGSVYFDL NSYQQRYSYP LFKSKDGINS EGDENKGKRS PCDFVLWKRS KENEPRYESK WGHGRPGWHI ECSVMSSDIL GXDLDIHAGG VDLAFPHHEN EIAQCQAYFM QEPWVKCFLH TGHLNISGLK MSKSLKNFTT IKEALKTISP RQLRVLFLHH QWNKDMNYEK EHLKFAETIE KKIFNFMSVA ESMRKNALAF ETLENADREV LRELGNVQEA VHAALLDNVD TPAVMKRIVE MINFTNARIK TISPSTVLVV KDYIKEITDV LGLSEEERQE SPGEDLIAQL LSNFRESIRE MARRKEPYSK FLEKCDWIRE SIKDYGYIIE DNSEGSILR
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaagact ctgaagctct acaattcgat taccaggacc attgatatct ttactcctag aaaaggcaac gaggtcaaga tgtatatatg cgggccaact gtgtacgact cttcccacat aggacatgca aggacctatg tgatgcttga tgtcatacgg cgtgtcctga gcgactatct gaagtataat gtgagatttg tgatgaacat aacagacatc gatgacaaga taatagccag agccaatgag actggggcat ccatggaaga agttaccagg aaatacactg aagagttttt tgaggacatg aagaccttga atgtcaggag cccctcgttt gtgacgtttg tgacgtcgta tgtcgacaag attgtgaagt ttattgaaaa gcttgaggca aacgggcttg catacgaaag cagagggagt gtgtactttg atctgaacag ttatcagcag agatacagct accctctatt caagagcaag gacggcatca actctgaagg agatgaaaac aagggcaaaa gaagcccgtg cgactttgtc ctctggaaga ggtccaagga aaatgagcca cggtatgagt cgaaatgggg acatgggagg cctggatggc atattgagtg cagtgtaatg tccagtgata ttctcggana agacctcgac atacatgctg gaggagtcga cctngcattt ccacaccacg agaacgagat agctcagtgc caagcctact ttatgcagga gccctgggtg aagtgctttc tccacacagg gcatctgaac atcagcgggc tgaagatgtc caagtccctg aagaacttta caacgattaa ggaagcactg aaaacgattt cccctcgcca gctaagggtt ctgtttctac accatcagtg gaacaaggac atgaactacg agaaagagca cctgaagttt gcagaaacca ttgagaagaa gatcttcaac tttatgtctg tggcggagag tatgagaaag aacgcactgg catttgagac tctagaaaat gcggacaggg aagttctgag ggagctaggg aatgtccagg aagctgtgca tgcagcactt cttgacaatg ttgacacgcc tgctgtaatg aagaggatag tggagatgat aaattttaca aatgccagaa taaagacaat ctcaccgtct accgttctgg ttgtcaagga ctacatcaag gagatcacag acgtgcttgg attgagtgag gaggagagac aggaatcccc tggagaggat ctgattgccc aactgcttag caacttcaga gaaagtatta gggagatggc aagacgaaag gagccttatt cgaagttcct tgagaagtgc gattggatcc gagaaagcat caaggactac ggatatatta tcgaagataa cagtgaagga tctatactga gaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDKTLKLYN SITRTIDIFT PRKGNEVKMY ICGPTVYDSS HIGHARTYVM FDVIRRVLSD YLKYNVRFVM NITDIDDKII ARANETGASM EEVTRKYTEE FFEDMKTLNV RSPSFVTFVT SYVDKIVKFI EKLEANGLAY ESRGSVYFDL NSYQQRYSYP LFKSKDGINS EGDENKDKRS PCDFVLWKRS KENEPRYESK WGHGRPGWHI ECSVMSSDIL GEDLDIHAGG VDLAFPHHEN EIAQCQAYFM QEPWVKCFLH TGHLNISGLK MSKSLKNFTT IKEALKTISP RQLRVLFLHH QWNKDMNYEK EHLKFAETIE KKIFNFMSVA ESMRKNALAF ETLENADREV LRELGNVQEA VHAALLDNVD TPAVMKRIVE MINFTNARIK TISPSTVLVV KDYIKEITDV LGLSEEERQE SPGEDLIAQL LSNFRESIRE MARRKEPYSK FLEKCDWIRE SIKDYGYIIE DNSEGSILRK K
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACAAGACT CTGAAGCTCT ACAATTCGAT TACCAGGACC ATTGATATCT TTACTCCTAG AAAAGGCAAC GAGGTCAAGA TGTATATATG CGGGCCAACT GTGTACGACT CTTCCCACAT AGGACATGCA AGGACCTATG TGATGTTTGA TGTCATACGG CGTGTCCTGA GCGACTATCT GAAGTATAAT GTGAGATTTG TGATGAACAT AACAGACATC GATGACAAGA TAATAGCCAG AGCCAATGAG ACTGGGGCAT CCATGGAAGA AGTTACCAGG AAATACACTG AAGAGTTTTT TGAGGACATG AAGACCTTGA ATGTCAGGAG CCCCTCGTTT GTGACGTTTG TGACGTCGTA TGTCGACAAG ATTGTGAAGT TTATTGAAAA GCTTGAGGCA AACGGGCTTG CATACGAAAG CAGAGGGAGT GTGTACTTTG ATCTGAACAG TTATCAGCAG AGATACAGCT ACCCTCTATT CAAGAGCAAG GACGGCATCA ACTCTGAAGG AGATGAAAAC AAGGACAAAA GAAGCCCGTG CGACTTTGTC CTCTGGAAGA GGTCCAAGGA AAATGAGCCA CGGTATGAGT CGAAATGGGG ACATGGGAGG CCTGGATGGC ATATTGAGTG CAGTGTAATG TCCAGTGATA TTCTCGGAGA AGACCTCGAC ATACATGCTG GAGGAGTCGA CCTTGCATTT CCACACCACG AGAACGAGAT AGCTCAGTGC CAAGCCTACT TTATGCAGGA GCCCTGGGTG AAGTGCTTTC TCCACACAGG GCATCTGAAC ATCAGCGGGC TGAAGATGTC CAAGTCCCTG AAGAACTTTA CAACGATTAA GGAAGCACTG AAAACGATTT CCCCTCGCCA GCTAAGGGTT CTGTTTCTAC ACCATCAGTG GAACAAGGAC ATGAACTACG AGAAAGAGCA CCTGAAGTTT GCAGAAACCA TTGAGAAGAA GATCTTCAAC TTTATGTCTG TGGCGGAGAG TATGAGAAAG AACGCACTGG CATTTGAGAC TCTAGAAAAT GCGGACAGGG AAGTTCTGAG GGAGCTAGGG AATGTCCAGG AAGCTGTGCA TGCAGCACTT CTTGACAATG TTGACACGCC TGCTGTAATG AAGAGGATAG TGGAGATGAT AAATTTTACA AATGCCAGAA TAAAGACAAT CTCACCGTCT ACCGTTCTGG TTGTCAAGGA CTACATCAAG GAGATCACAG ACGTGCTTGG ATTGAGTGAG GAGGAGAGAC AGGAATCCCC TGGAGAGGAT CTGATTGCCC AACTGCTTAG CAACTTCAGA GAAAGTATTA GGGAGATGGC AAGACGAAAG GAGCCTTATT CGAAGTTCCT TGAGAAGTGC GATTGGATCC GAGAAAGCAT CAAGGACTAC GGATATATTA TCGAAGATAA CAGTGAAGGA TCTATACTGA GAAAGAAAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA