SSGCID: Target Page

EncuA.00782.a

Ribonuclease

CENTER ID:	EncuA.00782.a
ORGANISM:	Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE:	Microsporidiosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EncuA.00782.a.A1.GE27232	Full length( EncuA.00782.a )		1	239

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	MicrosporidiaDB:ECU03_0670
RefSeq:	NP_597578.1
UniProt:	Q8SSA4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MESKNIFFSS FGQIEEEVVV GIDEAGRGPV VGYMVYGALV VPRGVLEDVG FKDSKVLTPA QRYNFFQMIK KKGFGYAYHC SHPDYITEMM QVRSVSLNEV SIGSVLRILD EVQSKCRRVD TIYVDALGDC RKYKERLEKD YPYRFVVEER ADSKFQVVSG ASIVAKVQRD MLISEFGEDL GSGYPSDPET IRWLRKNINV VFGFPPGVRY SWATVKKMLG ERKSRPLKGA LSGFYLDST

NT Sequence

ATGGAAAGTA AGAACATTTT TTTCTCGAGC TTCGGACAAA TCGAGGAGGA GGTGGTGGTC GGGATCGATG AGGCGGGCAG AGGTCCTGTG GTTGGGTACA TGGTGTATGG TGCATTGGTT GTTCCAAGAG GTGTGCTGGA GGATGTGGGA TTCAAGGACT CGAAGGTCCT AACGCCTGCA CAAAGATACA ATTTCTTCCA GATGATAAAG AAGAAGGGCT TTGGATATGC TTACCATTGC AGCCATCCGG ACTACATCAC AGAAATGATG CAAGTAAGGT CTGTAAGCCT AAACGAGGTG TCGATTGGAT CTGTTCTAAG AATCCTTGAC GAAGTACAAA GCAAGTGCAG AAGAGTAGAT ACCATCTATG TGGATGCACT GGGTGATTGT AGGAAGTACA AGGAAAGGCT TGAAAAGGAC TATCCATACA GATTCGTGGT AGAAGAACGG GCAGACTCGA AGTTTCAGGT TGTCTCTGGA GCCAGCATAG TTGCCAAGGT CCAGAGAGAC ATGCTGATTT CAGAGTTTGG GGAAGACCTC GGGTCTGGAT ATCCAAGTGA CCCAGAAACA ATTAGATGGC TCAGAAAAAA CATCAATGTG GTCTTTGGAT TTCCGCCCGG GGTCAGGTAC TCATGGGCTA CAGTCAAGAA AATGCTTGGG GAGAGAAAGT CGAGACCACT CAAGGGAGCC TTAAGCGGGT TCTATCTCGA TAGCACC

Details for EncuA.00782.a.A1.GE27232

HARVESTED ON:	7/31/2009
SEQUENCED ON:	3/21/2012
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	91

Validated AA Sequence

Validated NT Sequence

ggtaccctgg aagctcagac ccagggtcct ggttcgatgg aaagtaagaa catttttttc tcgagcttcg gacaaatcga ggaggaggtg gtggtcggga tcgatgaggc gggcagaggt cctgtggttg ggtacatggt gtatggtgca ttggttgttc caagaggtgt gctggaggat gtgggattca aggactcgaa ggtcctaacg cctgcacaaa gatacaattt cttccagatg ataaagaaga agggctttgg atatgcttac cattgcagcc atccggacta catcacagaa atgatgcaag taaggtctgt aagcctaaac gaggtgtcga ttggatctgt tctaagaatc cttgacgaag tacaaagcaa gtgcagaaga gtagatacca tctatgtgga tgcactgggt gattgtagga agtacaagga aaggcttgaa aaggactatc catacagatt cgtggtagaa gaacgggcag actcgaagtt tcaggttgtc tctggagcca gcatagttgc caaggtccag agagacatgc tgatttcaga gtttggggaa gacctcgggt ctggatatcc aagtgaccca gaaacaatta gatggctcag aaaaaacatc aatgtggtct ttggatttcc gcccggggtc aggtactcat gggctacagt caagaaaatg cttgnggaga gaaagtcgag accactcaag ggagccttaa gcgggttcta tctcgatagc acctaataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMESKNIFFS SFGQIEEEVV VGIDEAGRGP VVGYMVYGAL VVPRGVLEDV GFKDSKVLTP AQRYNFFQMI KKKGFGYAYH CSHPDYITEM MQVRSVSLNE VSIGSVLRIL DEVQSKCRRV DTIYVDALGD CRKYKERLEK DYPYRFVVEE RADSKFQVVS GASIVAKVQR DMLISEFGED LGSGYPSDPE TIRWLRKNIN VVFGFPPGVR YSWATVKKML GERKSRPLKG ALSGFYLDST

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaaagtaag aacatttttt tctcgagctt cggacaaatc gaggaggagg tggtggtcgg gatcgatgag gcgggcagag gtcctgtggt tgggtacatg gtgtatggtg cattggttgt tccaagaggt gtgctggagg atgtgggatt caaggactcg aaggtcctaa cgcctgcaca aagatacaat ttcttccaga tgataaagaa gaagggcttt ggatatgctt accattgcag ccatccggac tacatcacag aaatgatgca agtaaggtct gtaagcctaa acgaggtgtc gattggatct gttctaagaa tccttgacga agtacaaagc aagtgcagaa gagtagatac catctatgtg gatgcactgg gtgattgtag gaagtacaag gaaaggcttg aaaaggacta tccatacaga ttcgtggtag aagaacgggc agactcgaag tttcaggttg tctctggagc cagcatagtt gccaaggtcc agagagacat gctgatttca gagtttgggg aagacctcgg gtctggatat ccaagtgacc cagaaacaat tagatggctc agaaaaaaca tcaatgtggt ctttggattt ccgcccgggg tcaggtactc atgggctaca gtcaagaaaa tgcttgggga gagaaagtcg agaccactca agggagcctt aagcgggttc tatctcgata gcaccaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa