EncuA.00739.a

Serine/threonine protein phosphatase

CENTER ID: EncuA.00739.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00739.a.A1.GE27808 Full length( EncuA.00739.a ) 1 311
EncuA.00739.a.A1.GE28322 Full length( EncuA.00739.a ) 1 311
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU09_0980
RefSeq: XP_955652.1
UniProt: Q8SQM9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYLVFNPSPM KRLVSKLLNL ELLSEAEVQG ICNKVSEILM REPNVPNKRA PVIVCGDIHG QFFDLVSLFR IDGIPSGKRY IFLGDYVDRG RNSLECILLL LLLKILYPEN VTLIRGNHEQ STINRVYGFY EEIVEKYGNP RIWRMINEVF SLMCIGCLVD GRILCVHGGI SPKIMSLNQI RRLDRLQPIG EGSEFADILW GDPYEGRGFQ ASPRGSGYLY GEDVVIRFLE LNGLISIVRS HQLVIEGYKF HFPERNVITV WSAPNYMGKC GNPASILRIN ETLDVSDKDF CIFKATPQRK EFLEKLEGFN L
NT Sequence
ATGTATTTGG TTTTTAATCC ATCCCCAATG AAAAGACTCG TGTCCAAGCT TCTGAACCTC GAGCTGCTTT CCGAGGCAGA GGTCCAGGGC ATCTGCAACA AGGTGTCGGA GATTCTTATG CGTGAGCCAA ACGTCCCAAA CAAAAGGGCT CCTGTCATTG TTTGTGGAGA TATCCATGGA CAGTTCTTTG ATCTTGTAAG CCTGTTCAGA ATCGACGGAA TCCCCTCGGG GAAGAGGTAC ATCTTCCTTG GGGACTATGT CGACAGGGGG AGAAACTCGC TTGAGTGCAT ACTGCTCCTT CTTCTCCTGA AGATCCTGTA TCCAGAAAAT GTCACTTTGA TAAGGGGGAA TCACGAGCAG TCCACCATCA ACAGGGTGTA TGGCTTCTAC GAGGAGATTG TTGAGAAGTA TGGCAACCCG AGGATATGGA GGATGATCAA CGAGGTGTTT AGCCTGATGT GCATTGGATG CTTAGTTGAT GGAAGAATAT TGTGTGTGCA TGGAGGCATA TCGCCTAAGA TCATGTCTCT GAACCAAATA AGGAGACTCG ACAGGCTGCA GCCCATTGGA GAGGGAAGCG AGTTTGCAGA CATCCTGTGG GGAGATCCCT ATGAGGGAAG AGGTTTCCAG GCCAGCCCTC GGGGAAGCGG ATATCTTTAT GGAGAGGATG TGGTAATAAG GTTTCTTGAG CTGAACGGAC TTATAAGCAT CGTAAGATCC CATCAGCTTG TCATAGAGGG CTACAAGTTC CACTTTCCAG AGAGGAACGT GATTACTGTG TGGAGTGCCC CCAATTACAT GGGGAAGTGC GGAAATCCAG CAAGTATTCT TCGGATCAAC GAGACGTTGG ATGTTTCTGA CAAGGACTTC TGTATATTCA AGGCCACGCC ACAGAGAAAG GAGTTTCTTG AGAAGCTCGA GGGATTCAAC TTG
Details for EncuA.00739.a.A1.GE27808
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 37kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMYLVFNPSP MKRLVSKLLN LELLSEAEVQ GICNKVSEIL MREPNVPNKR APVIVCGDIH GQFFDLVSLF RIDGIPSGKR YIFLGDYVDR GRNSLECILL LLLLKILYPE NVTLIRGNHE QSTINRVYGF YEEIVEKYGN PRIWRMINEV FSLMCIGCLV DGRILCVHGG ISPKIMSLNQ IRRLDRLQPI GEGSEFADIL WGDPYEGRGF QASPRGSGYL YGEDVVIRFL ELNGLISIVR SHQLVIEGYK FHFPERNVIT VWSAPNYMGK CGNPASILRI NETLDVSDKD FCIFKATPQR KEFLEKLEGF
Validated NT Sequence
tgaatccctc gagcttctca agaaactcct ttctctgtgg cgtggccttg aatatacaga agtccttgtc agaaacatcc aacgtctcgt tgatccgaag aatacttgct ggatttccgc acttccccat gtaattgggg gcactccaca cagtaatcac gttcctctct ggaaagtgga acttgtagcc ctctatgaca agctgatggg atcttacgat gcttataagt ccgttcagct caagaaacct tattaccaca tcctctccat aaagatatcc gcttccccga gggctggcct ggaaacctct tccctcatag ggatctcccc acaggatgtc tgcaaactcg cttccctctc caatgggctg cagcctgtcg agcctcctta tttggttcag agacatgatc ttaggcgata tgcctccatg cacacacaat attcttccat caactaagca tccaatgcac atcaggctaa acacctcgtt gatcatcctc catatcctcg ggttgccata cttctcaaca atctcctcgt agaagccata caccctgttg atggtggact gctcgtgatt cccccttatc aaagtgacat tttctggata caggatcttc aggagaagaa ggagcagtat gcactcaagc gagtttctcc ccctgtcgac atagtcccca aggaagatgt acctcttccc cgaggggatt ccgtcgattc tgaacaggct tacaagatca aagaactgtc catggatatc tccacaaaca atgacaggag cccttttgtt tgggacgttt ggctcacgca taagaatctc cgacaccttg ttgcagatgc cctggacctc tgcctcggaa agcagctcga ggttcagaag cttggacacg agtcttttca ttggggatgg attaaaaacc aaatacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMYLVFNPSP MKRLVSKLLN LELLSEAEVQ GICNKVSEIL MREPNVPNKR APVIVCGDIH GQFFDLVSLF RIDGIPSGKR YIFLGDYVDR GRNSLECILL LLLLKILYPE NVTLIRGNHE QSTINRVYGF YEEIVEKYGN PRIWRMINEV FSLMCIGCLV DGRILCVHGG ISPKIMSLNQ IRRLDRLQPI GEGSEFADIL WGDPYEGRGF QASPRGSGYL YGEDVVIRFL ELNGLISIVR SHQLVIEGYK FHFPERNVIT VWSAPNYMGK CGNPASILRI NETLDVSDKD FCIFKATPQR KEFLEKLEGF NL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtatttggtt tttaatccat ccccaatgaa aagactcgtg tccaagcttc tgaacctcga gctgctttcc gaggcagagg tccagggcat ctgcaacaag gtgtcggaga ttcttatgcg tgagccaaac gtcccaaaca aaagggctcc tgtcattgtt tgtggagata tccatggaca gttctttgat cttgtaagcc tgttcagaat cgacggaatc ccctcgggga agaggtacat cttccttggg gactatgtcg acagggggag aaactcgctt gagtgcatac tgctccttct tctcctgaag atcctgtatc cagaaaatgt cactttgata agggggaatc acgagcagtc caccatcaac agggtgtatg gcttctacga ggagattgtt gagaagtatg gcaacccgag gatatggagg atgatcaacg aggtgtttag cctgatgtgc attggatgct tagttgatgg aagaatattg tgtgtgcatg gaggcatatc gcctaagatc atgtctctga accaaataag gagactcgac aggctgcagc ccattggaga gggaagcgag tttgcagaca tcctgtgggg agatccctat gagggaagag gtttccaggc cagccctcgg ggaagcggat atctttatgg agaggatgtg gtaataaggt ttcttgagct gaacggactt ataagcatcg taagatccca tcagcttgtc atagagggct acaagttcca ctttccagag aggaacgtga ttactgtgtg gagtgccccc aattacatgg ggaagtgcgg aaatccagca agtattcttc ggatcaacga gacgttggat gtttctgaca aggacttctg tatattcaag gccacgccac agagaaagga gtttcttgag aagctcgagg gattcaactt gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for EncuA.00739.a.A1.GE28322
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMYLVFNPSP MKRLVSKLLN LELLSEAEVQ GICNKVSEIL MREPNVPNKR APVIVCGDIH GQFFDLVSLF RIDGIPSGKR YIFLGDYVDR GRNSLECILL LLLLKILYPE NVTLIRGNHE QSTINRVYGF YEEIVEKYGN PRIWRMINEV FSLMCIGCLV DGRILCVHGG ISPKIMSLNQ IRRLDRLQPI GEGSEFADIL WGDPYEGRGF QASPRGSGYL YGEDVVIRFL ELNGLISIVR SHQLVIEGYK FHFPERNVIT VWSAPNYMGK CGNPASILRI NETLDVSDKD FCIFKATPQR KEFLEKLEGF
Validated NT Sequence
tgaatccctc gagcttctca agaaactcct ttctctgtgg cgtggccttg aatatacaga agtccttgtc agaaacatcc aacgtctcgt tgatccgaag aatacttgct ggatttccgc acttccccat gtaattgggg gcactccaca cagtaatcac gttcctctct ggaaagtgga acttgtagcc ctctatgaca agctgatggg atcttacgat gcttataagt ccgttcagct caagaaacct tattaccaca tcctctccat aaagatatcc gcttccccga gggctggcct ggaaacctct tccctcatag ggatctcccc acaggatgtc tgcaaactcg cttccctctc caatgggctg cagcctgtcg agcctcctta tttggttcag agacatgatc ttaggcgata tgcctccatg cacacacaat attcttccat caactaagca tccaatgcac atcaggctaa acacctcgtt gatcatcctc catatcctcg ggttgccata cttctcaaca atctcctcgt agaagccata caccctgttg atggtggact gctcgtgatt cccccttatc aaagtgacat tttctggata caggatcttc aggagaagaa ggagcagtat gcactcaagc gagtttctcc ccctgtcgac atagtcccca aggaagatgt acctcttccc cgaggggatt ccgtcgattc tgaacaggct tacaagatca aagaactgtc catggatatc tccacaaaca atgacaggag cccttttgtt tgggacgttt ggctcacgca taagaatctc cgacaccttg ttgcagatgc cctggacctc tgcctcggaa agcagctcga ggttcagaag cttggacacg agtcttttca ttggggatgg attaaaaacc aaatacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMYLVFNPSP MKRLVSKLLN LELLSEAEVQ GICNKVSEIL MREPNVPNKR APVIVCGDIH GQFFDLVSLF RIDGIPSGKR YIFLGDYVDR GRNSLECILL LLLLKILYPE NVTLIRGNHE QSTINRVYGF YEEIVEKYGN PRIWRMINEV FSLMCIGCLV DGRILCVHGG ISPKIMSLNQ IRRLDRLQPI GEGSEFADIL WGDPYEGRGF QASPRGSGYL YGEDVVIRFL ELNGLISIVR SHQLVIEGYK FHFPERNVIT VWSAPNYMGK CGNPASILRI NETLDVSDKD FCIFKATPQR KEFLEKLEGF NL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtatttggtt tttaatccat ccccaatgaa aagactcgtg tccaagcttc tgaacctcga gctgctttcc gaggcagagg tccagggcat ctgcaacaag gtgtcggaga ttcttatgcg tgagccaaac gtcccaaaca aaagggctcc tgtcattgtt tgtggagata tccatggaca gttctttgat cttgtaagcc tgttcagaat cgacggaatc ccctcgggga agaggtacat cttccttggg gactatgtcg acagggggag aaactcgctt gagtgcatac tgctccttct tctcctgaag atcctgtatc cagaaaatgt cactttgata agggggaatc acgagcagtc caccatcaac agggtgtatg gcttctacga ggagattgtt gagaagtatg gcaacccgag gatatggagg atgatcaacg aggtgtttag cctgatgtgc attggatgct tagttgatgg aagaatattg tgtgtgcatg gaggcatatc gcctaagatc atgtctctga accaaataag gagactcgac aggctgcagc ccattggaga gggaagcgag tttgcagaca tcctgtgggg agatccctat gagggaagag gtttccaggc cagccctcgg ggaagcggat atctttatgg agaggatgtg gtaataaggt ttcttgagct gaacggactt ataagcatcg taagatccca tcagcttgtc atagagggct acaagttcca ctttccagag aggaacgtga ttactgtgtg gagtgccccc aattacatgg ggaagtgcgg aaatccagca agtattcttc ggatcaacga gacgttggat gtttctgaca aggacttctg tatattcaag gccacgccac agagaaagga gtttcttgag aagctcgagg gattcaactt gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa