EncuA.00696.a

PHENYLALANYL tRNA SYNTHETASE BETA SUBUNIT

CENTER ID: EncuA.00696.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00696.a.A1.GE27224 Full length( EncuA.00696.a ) 1 475
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU07_1660
RefSeq: NP_586093.1
UniProt: Q8SRG9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAVAQKIIE NLKMQDEVRS SDIDAPYSEI HGALISLESR GICNVKMEES YEEVLTPEGE EVERSGSQEY LLLESIGEDG MSIEELERHS LGKMNAFRNK WIRREGNRVF RNVESIEDTV RNMVISMKRG TASAEEVEAL RKRKLVSRRK KIVFIATKGP MFFDNTSYVT ELTSEMVLDG SYRDLSFKHY NFNAEGNAPQ CGSIHPLMKI REDFRRIFIE LGFSEMATNQ YVESSFWNFD ALFQPQNHPS RDAHDTFFLL NPELSTDFPS DYLKEVGETH RGGKYNSLGY MSNWDIKEAQ KNILRTHTTS VSARNLYRLA KKEFKPAKLF SIDKVFRNET VDATHLAEFH QVEGLVVGKG LNLTHLMGIL REFFNRLGMG DIRFKPAFNP YTEPSMEVFG YHKGMDRWIE VGNSGVFRPE MLRPMGFDSD VSVVAWGLSL ERPAMIKYGL KNIRELVGHK VDIEFSRKSE ICFFD
NT Sequence
ATGACTGCAG TAGCGCAAAA GATAATAGAG AATTTGAAAA TGCAGGACGA GGTAAGATCT TCTGACATAG ACGCACCCTA CTCAGAGATA CACGGGGCCT TGATAAGTCT TGAGAGCAGG GGGATATGCA ACGTGAAGAT GGAGGAATCA TATGAGGAGG TACTAACTCC GGAAGGCGAG GAAGTGGAAA GAAGTGGGAG CCAGGAGTAT CTGTTGCTTG AATCAATAGG AGAAGACGGT ATGAGTATTG AGGAGCTAGA AAGGCATAGC CTTGGGAAAA TGAATGCCTT TAGGAATAAA TGGATAAGGA GAGAAGGGAA CAGGGTTTTC AGGAATGTGG AGAGTATTGA GGATACGGTT CGAAACATGG TGATCAGCAT GAAGAGGGGA ACAGCAAGCG CTGAGGAAGT TGAGGCACTA AGGAAGAGGA AGCTGGTATC CAGAAGGAAG AAGATTGTAT TTATAGCCAC TAAGGGGCCT ATGTTCTTTG ACAACACTTC ATATGTCACT GAACTAACGT CTGAGATGGT CCTCGATGGA TCCTATAGGG ACCTAAGCTT CAAGCATTAT AACTTTAATG CCGAAGGAAA TGCTCCCCAA TGTGGATCGA TTCATCCTCT TATGAAGATA AGAGAAGATT TCAGAAGAAT ATTTATTGAG CTAGGGTTTA GTGAGATGGC AACCAATCAG TATGTGGAGT CATCCTTCTG GAATTTCGAT GCGTTATTCC AGCCCCAGAA TCATCCATCC AGAGATGCAC ACGATACTTT TTTTCTTCTG AATCCAGAGC TAAGCACGGA CTTTCCCTCG GATTATCTCA AAGAGGTTGG GGAGACCCAT CGCGGAGGAA AATATAACTC GTTGGGATAC ATGAGTAACT GGGACATTAA GGAGGCACAG AAGAACATCT TGAGAACGCA CACAACGTCT GTCAGTGCTA GAAACCTGTA TAGACTTGCA AAGAAGGAAT TCAAGCCAGC AAAGCTCTTC TCGATTGACA AGGTCTTCCG AAATGAAACT GTTGATGCAA CGCACCTCGC CGAGTTCCAT CAAGTGGAGG GACTAGTCGT TGGTAAGGGC TTGAACTTAA CTCATCTAAT GGGGATTCTT CGGGAGTTCT TCAACAGACT GGGGATGGGC GACATCCGAT TCAAACCTGC ATTCAATCCA TATACTGAGC CTTCGATGGA GGTGTTTGGG TATCACAAGG GAATGGACCG GTGGATAGAG GTTGGAAACT CCGGGGTATT CCGGCCGGAA ATGCTAAGGC CAATGGGCTT TGATTCTGAT GTGAGTGTTG TTGCATGGGG GCTATCTCTA GAAAGACCTG CCATGATAAA GTACGGGCTA AAGAATATCC GAGAACTTGT TGGGCATAAG GTTGACATTG AGTTTTCAAG GAAAAGTGAA ATATGCTTCT TCGAT
Details for EncuA.00696.a.A1.GE27224
HARVESTED ON: 7/31/2009
SEQUENCED ON: 8/12/2009
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAXTQG PGSMTAVAQK IIENLKMQDE VRSSDIDAPY SEIHGALISL ESRGICNVKM EESYEEVLTP EGEEVERSGS QEYLLLESIG EDGMSIEELE RHSLGKINAF RNKWIRREGN RVFRNVESIE DTVRNMVISM KRGTASAEEV EALRKRKLVS RRKKIVFIAT KGPMFFDNTS YVTELTSEMV LDGSYRDLSF KHYNFNAEGN APQCGSIHPL MKIREDFRRI FIELGFSEMA TNQYVESSFW NFDALFQPQN HPSRDAHDTF FLLNPELSTD FPSDYLKEVG ETHRGGKYNS LGYMSNWDIK EAQKNILRTH TTSVSARNLY RLAKKEFKPA KLFSIDKVFR NETVDATHLA EFHQVEGLVV GKGLNLTHLM GILREFFNRL GMGDIRFKPA FNPYTEPSME VFGYHKGMDR WIEVGNSGVF RPEMLRPMGF DSDVSVVAWG LSLERPAMIK YGLKNIRELV GHKVDIEFSR KSEICFFD
Validated NT Sequence
ttattaatcg aagaagcata tttcactttt ccttgaaaac tcaatgtcaa ccttatgccc aacaagttct cggatattct ttagcccgta ctttatcatg gcaggtcttt ctagagatag cccccatgca acaacactca catcagaatc aaagcccatt ggccttagca tttccggccg gaataccccg gagtttccaa cctctatcca ccggtccatt cccttgtgat acccaaacac ctccatcgaa ggctcagtat atggattgaa tgcaggtttg aatcggatgt cgcccatccc cagtctgttg aagaactccc gaagaatccc cattagatga gttaagttca agcccttacc aacgactagt ccctccactt gatggaactc ggcgaggtgc gttgcatcaa cagtttcatt tcggaagacc ttgtcaatcg agaagagctt tgctggcttg aattccttct ttgcaagtct atacaggttt ctagcactga cagacgttgt gtgcgttctc aagatgttct tctgtgcctc cttaatgtcc cagttactca tgtatcccaa cgagttatat tttcctccgc gatgggtctc cccaacctct ttgagataat ccgagggaaa gtccgtgctt agctctggat tcagaagaaa aaaagtatcg tgtgcatctc tggatggatg attctggggc tggaataacg catcgaaatt ccagaaggat gactccacat actgattggt tgccatctca ctaaacccta gctcaataaa tattcttctg aaatcttctc ttatcttcat aagaggatga atcgatccac attggggagc atttccttcg gcattaaagt tataatgctt gaagcttagg tccctatagg atccatcgag gaccatctca gacgttagtt cagtgacata tgaagtgttg tcaaagaaca taggcccctt agtggctata aatacaatct tcttccttct ggataccagc ttcctcttcc ttagtgcctc aacttcctca gcgcttgctg ttcccctctt catgctgatc accatgtttc gaaccgtatc ctcaatactc tccacattcc tgaaaaccct gttcccttct ctccttatcc atttattcct aaaggcattt attttcccaa ggctatgcct ttctagctcc tcaatactca taccgtcttc tcctattgat tcaagcaaca gatactcctg gctcccactt ctttccactt cctcgccttc cggagttagt acctcctcat atgattcctc catcttcacg ttgcatatcc ccctgctctc aagacttatc aaggccccgt gtatctctga gtagggtgcg tctatgtcag aagatcttac ctcgtcctgc attttcaaat tctctattat cttttgcgct actgcagtca tcgaaccagg accctgggtn tgagcttcca gggtacccat atg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAVAQKII ENLKMQDEVR SSDIDAPYSE IHGALISLES RGICNVKMEE SYEEVLTPEG EEVERSGSQE YLLLESIGED GMSIEELERH SLGKMNAFRN KWIRREGNRV FRNVESIEDT VRNMVISMKR GTASAEEVEA LRKRKLVSRR KKIVFIATKG PMFFDNTSYV TELTSEMVLD GSYRDLSFKH YNFNAEGNAP QCGSIHPLMK IREDFRRIFI ELGFSEMATN QYVESSFWNF DALFQPQNHP SRDAHDTFFL LNPELSTDFP SDYLKEVGET HRGGKYNSLG YMSNWDIKEA QKNILRTHTT SVSARNLYRL AKKEFKPAKL FSIDKVFRNE TVDATHLAEF HQVEGLVVGK GLNLTHLMGI LREFFNRLGM GDIRFKPAFN PYTEPSMEVF GYHKGMDRWI EVGNSGVFRP EMLRPMGFDS DVSVVAWGLS LERPAMIKYG LKNIRELVGH KVDIEFSRKS EICFFD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgcagta gcgcaaaaga taatagagaa tttgaaaatg caggacgagg taagatcttc tgacatagac gcaccctact cagagataca cggggccttg ataagtcttg agagcagggg gatatgcaac gtgaagatgg aggaatcata tgaggaggta ctaactccgg aaggcgagga agtggaaaga agtgggagcc aggagtatct gttgcttgaa tcaataggag aagacggtat gagtattgag gagctagaaa ggcatagcct tgggaaaatg aatgccttta ggaataaatg gataaggaga gaagggaaca gggttttcag gaatgtggag agtattgagg atacggttcg aaacatggtg atcagcatga agaggggaac agcaagcgct gaggaagttg aggcactaag gaagaggaag ctggtatcca gaaggaagaa gattgtattt atagccacta aggggcctat gttctttgac aacacttcat atgtcactga actaacgtct gagatggtcc tcgatggatc ctatagggac ctaagcttca agcattataa ctttaatgcc gaaggaaatg ctccccaatg tggatcgatt catcctctta tgaagataag agaagatttc agaagaatat ttattgagct agggtttagt gagatggcaa ccaatcagta tgtggagtca tccttctgga atttcgatgc gttattccag ccccagaatc atccatccag agatgcacac gatacttttt ttcttctgaa tccagagcta agcacggact ttccctcgga ttatctcaaa gaggttgggg agacccatcg cggaggaaaa tataactcgt tgggatacat gagtaactgg gacattaagg aggcacagaa gaacatcttg agaacgcaca caacgtctgt cagtgctaga aacctgtata gacttgcaaa gaaggaattc aagccagcaa agctcttctc gattgacaag gtcttccgaa atgaaactgt tgatgcaacg cacctcgccg agttccatca agtggaggga ctagtcgttg gtaagggctt gaacttaact catctaatgg ggattcttcg ggagttcttc aacagactgg ggatgggcga catccgattc aaacctgcat tcaatccata tactgagcct tcgatggagg tgtttgggta tcacaaggga atggaccggt ggatagaggt tggaaactcc ggggtattcc ggccggaaat gctaaggcca atgggctttg attctgatgt gagtgttgtt gcatgggggc tatctctaga aagacctgcc atgataaagt acgggctaaa gaatatccga gaacttgttg ggcataaggt tgacattgag ttttcaagga aaagtgaaat atgcttcttc gataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa