EncuA.00673.a

26S PROTEASOME REGULATORY SUBUNIT 4

CENTER ID: EncuA.00673.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00673.a.MB1.GE30458 Full length( EncuA.00673.a ) 1 424
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU07_1640
OrthoMCL: OG5_127456
RefSeq: NP_586091.1
UniProt: Q8SRH0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRDKSERDN LQDTTTINLR RRRRVKEGKA ASKPPQVYPL MKCKLRYLKL KKLAHLLSLE DNILSLCEPD KSSEGSNSQK HVVEQLRGSP LSVGTLEEFV DDHHGIITTG VGLEYYVNIM SFVDKDLLEP GCTVLLNYKD NSVVGVLEGE MDPMVNVMKL EKAPSETYAD IGGLEEQIQE IKESVELPLT NPELYQEMGI KPPKGVILYG LPGTGKTLLA KAVANQTSAT FLRVVGTELI QEYLGEGPKL VRELFRVADM HAPSIIFIDE IDAIGGKRYN TSSGGRREVQ RTMLELLNQL DGFDTRNDIK VIMATNKIEA LDPALIRPGR IDRKIEFGMP DAATKKKIFD IHTSRMTLDE SVNIELLITS KEDLSGADIK AICTEAGMIA LRERRKTVTM KDFISAREKV FFSKQKMVSA GLYS
NT Sequence
ATGAGCAGAG ACAAATCCGA GCGGGATAAC CTTCAGGACA CAACCACAAT AAACCTGAGG AGGCGCAGGA GAGTAAAGGA GGGCAAGGCA GCCTCCAAGC CGCCGCAGGT GTATCCATTG ATGAAGTGTA AGCTGAGATA TCTGAAGTTG AAGAAGCTGG CGCACTTATT ATCTTTAGAA GACAACATAC TGAGCTTATG CGAGCCAGAC AAGTCGTCCG AGGGATCGAA TAGCCAGAAG CATGTTGTTG AGCAGCTTCG CGGGAGCCCT CTTTCTGTCG GGACGCTTGA GGAGTTTGTC GACGACCACC ACGGAATAAT TACCACGGGT GTTGGGTTGG AATACTATGT CAACATCATG TCGTTTGTTG ACAAGGACCT ACTTGAGCCT GGATGCACTG TTCTCCTGAA CTACAAAGAC AATAGTGTTG TGGGCGTTCT GGAGGGGGAG ATGGATCCAA TGGTCAATGT CATGAAGTTG GAAAAGGCAC CTTCGGAGAC ATATGCGGAC ATCGGGGGGC TTGAGGAACA GATCCAAGAG ATAAAGGAAA GTGTTGAGCT TCCACTGACG AACCCCGAGC TTTACCAGGA AATGGGGATA AAGCCCCCAA AGGGAGTGAT TCTGTACGGA CTTCCTGGAA CAGGGAAGAC TCTCCTAGCC AAGGCCGTTG CAAACCAGAC ATCTGCAACT TTTCTTAGGG TTGTTGGGAC GGAGCTTATA CAGGAGTATC TCGGGGAAGG CCCGAAGCTT GTAAGGGAGC TCTTCAGGGT TGCAGACATG CATGCACCAT CAATAATTTT TATTGACGAG ATAGATGCAA TTGGAGGGAA AAGGTACAAT ACATCCTCTG GTGGGCGGAG GGAGGTCCAA AGGACGATGC TAGAGTTGCT GAACCAACTG GATGGATTTG ACACCAGGAA CGATATCAAG GTGATAATGG CCACGAATAA GATAGAAGCC CTTGACCCGG CTTTGATCAG GCCTGGCAGA ATCGATAGGA AAATCGAGTT TGGCATGCCG GATGCCGCTA CAAAGAAGAA AATATTCGAT ATTCATACAT CGAGGATGAC ACTGGATGAA AGTGTGAACA TCGAGCTACT AATAACGAGC AAGGAGGATT TGAGCGGAGC TGATATAAAG GCAATCTGCA CAGAGGCTGG TATGATAGCA TTGAGAGAAA GGAGAAAAAC AGTTACCATG AAGGACTTTA TTTCTGCAAG AGAAAAGGTA TTCTTTTCAA AGCAAAAGAT GGTTTCTGCT GGGCTTTATT CC
Details for EncuA.00673.a.MB1.GE30458
HARVESTED ON: 5/11/2010
SEQUENCED ON: 10/7/2010
EXPECTED MW: 92kDa
OBSERVED MW: 90kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 37
Validated AA Sequence
MSRDKSERDN LQDTTTINLR RRRRVKEGKA ASKPPQVYPL MKCKLRYLKL KKLAHLLSLE DNILSLCEPD KSSEGSNSQK HVVEQLRGSP LSVGTLEEFV DDHHGIITTG VGLEYYVNIM SFVDKDLLEP GCTVLLNYKD NSVVGVLEGE MDPMVNVMKL EKAPSETYAD IGGLEEQIQE IKESVELPLT NPELYQEMGI KPPKGVILYG LPGTGKTLLA KAVANQTSAT FLRVVGTELI QEYLGEXPKL VRELFRVADM HAPSIIFIDE IDAIGGKRYN TSSGGRREVQ XXMLXLLXXL XGLXPXTISX
Validated NT Sequence
cnnngnnctc agacccnggg tcctggttcg atgagcagag acaaatccga gcgggataac cttcaggaca caaccacaat aaacctgagg aggcgcagga gagtaaagga gggcaaggca gcctccaagc cgccgcaggt gtatccattg atgaagtgta agctgagata tctgaagttg aagaagctgg cgcacttatt atctttagaa gacaacatac tgagcttatg cgagccagac aagtcgtccg agggatcgaa tagccagaag catgttgttg agcagcttcg cgggagccct ctttctgtcg ggacgcttga ggagtttgtc gacgaccacc acggaataat taccacgggt gttgggttgg aatactatgt caacatcatg tcgtttgttg acaaggacct acttgagcct ggatgcactg ttctcctgaa ctacaaagac aatagtgttg tgggcgttct ggagggggag atggatccaa tggtcaatgt catgaagttg gaaaaggcac cttcggagac atatgcggac atcggggggc ttgaggaaca gatccaagag ataaaggaaa gtgttgagct tccactgacg aaccccgagc tttaccagga aatggggata aagcccccaa agggagtgat tctgtacgga cttcctggaa cagggaagac tctcctagcc aaggccgttg caaaccagac atctgcaact tttcttaggg ttgttgggac ggagcttata caggagtatc tcggggaang nccgaagctt gtaagggagc tcttcagggt tgcagacatg catgcaccat caataatttt tattgacgag atagatgcna ttggagggaa aaggtacaat acatcctctg gtgggcggag ggaggtccaa angncgatgc taganttgct gannnaactg gntggtttga naccnnnaac gatatcannn tgataatggn nncnaatann nnanaagccn nnganccggn tttgatcnnn cctnnnanan ngatagnaaa atnnagnnnn gncatgncnn ntnnnntncn annnnnaaan nnnnanannn ntnctnnnng nnnnnnctgn nnnnaannnn nnnnnnntcn nnctacnann nnnnnntnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntnnnnnn nnnnnnnnnn aannnnnnnn nnnnnnngg
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MSRDKSERDN LQDTTTINLR RRRRVKEGKA ASKPPQVYPL MKCKLRYLKL KKLAHLLSLE DNILSLCEPD KSSEGSNSQK HVVEQLRGSP LSVGTLEEFV DDHHGIITTG VGLEYYVNIM SFVDKDLLEP GCTVLLNYKD NSVVGVLEGE MDPMVNVMKL EKAPSETYAD IGGLEEQIQE IKESVELPLT NPELYQEMGI KPPKGVILYG LPGTGKTLLA KAVANQTSAT FLRVVGTELI QEYLGEGPKL VRELFRVADM HAPSIIFIDE IDAIGGKRYN TSSGGRREVQ RTMLELLNQL DGFDTRNDIK VIMATNKIEA LDPALIRPGR IDRKIEFGMP DAATKKKIFD IHTSRMTLDE SVNIELLITS KEDLSGADIK AICTEAGMIA LRERRKTVTM KDFISAREKV FFSKQKMVSA GLYS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatgag cagagacaaa tccgagcggg ataaccttca ggacacaacc acaataaacc tgaggaggcg caggagagta aaggagggca aggcagcctc caagccgccg caggtgtatc cattgatgaa gtgtaagctg agatatctga agttgaagaa gctggcgcac ttattatctt tagaagacaa catactgagc ttatgcgagc cagacaagtc gtccgaggga tcgaatagcc agaagcatgt tgttgagcag cttcgcggga gccctctttc tgtcgggacg cttgaggagt ttgtcgacga ccaccacgga ataattacca cgggtgttgg gttggaatac tatgtcaaca tcatgtcgtt tgttgacaag gacctacttg agcctggatg cactgttctc ctgaactaca aagacaatag tgttgtgggc gttctggagg gggagatgga tccaatggtc aatgtcatga agttggaaaa ggcaccttcg gagacatatg cggacatcgg ggggcttgag gaacagatcc aagagataaa ggaaagtgtt gagcttccac tgacgaaccc cgagctttac caggaaatgg ggataaagcc cccaaaggga gtgattctgt acggacttcc tggaacaggg aagactctcc tagccaaggc cgttgcaaac cagacatctg caacttttct tagggttgtt gggacggagc ttatacagga gtatctcggg gaaggcccga agcttgtaag ggagctcttc agggttgcag acatgcatgc accatcaata atttttattg acgagataga tgcaattgga gggaaaaggt acaatacatc ctctggtggg cggagggagg tccaaaggac gatgctagag ttgctgaacc aactggatgg atttgacacc aggaacgata tcaaggtgat aatggccacg aataagatag aagcccttga cccggctttg atcaggcctg gcagaatcga taggaaaatc gagtttggca tgccggatgc cgctacaaag aagaaaatat tcgatattca tacatcgagg atgacactgg atgaaagtgt gaacatcgag ctactaataa cgagcaagga ggatttgagc ggagctgata taaaggcaat ctgcacagag gctggtatga tagcattgag agaaaggaga aaaacagtta ccatgaagga ctttatttct gcaagagaaa aggtattctt ttcaaagcaa aagatggttt ctgctgggct ttattcctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa