EncuA.00649.a

Triosephosphate isomerase

CENTER ID: EncuA.00649.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00649.a.A1.GE27218 Full length( EncuA.00649.a ) 1 242
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00649.a.A1.PW27207 Full length( EncuA.00649.a ) 1 242

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU11_0230
RefSeq: NP_586329.1
UniProt: Q8SQZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKGNKLLAGN WKMSSSLQTF EIIKSFRANL FAGNDTFIAV PFPYIHVAKK MFPEYIKVGA QDCSQFKSGP YTGEVSASMV SEMGAEYVII GHSERRRWFG ETSATVVKKI RNARSEGLKI VVCIGEDEKC REDGRHLDVI KDQLLSLGEE IGGEGIDIAY EPVWAIGTGK VPTNDQIREV VSGIKRWGTG MGFVGRVIYG GSVSMLNCQT ILDVEEIDGF LIGGTSLKMD FCMISSEMST KT
NT Sequence
ATGAAGGGAA ACAAACTTCT TGCAGGAAAC TGGAAGATGA GTTCGTCGTT ACAAACTTTT GAGATAATTA AAAGCTTCCG AGCGAATCTC TTTGCCGGAA ACGATACGTT TATTGCCGTT CCGTTTCCTT ATATTCATGT CGCGAAGAAG ATGTTTCCTG AGTACATCAA GGTTGGAGCG CAGGATTGCA GTCAGTTTAA AAGTGGCCCG TACACAGGTG AAGTCTCTGC ATCCATGGTG AGCGAGATGG GTGCTGAGTA TGTGATAATC GGCCATTCTG AAAGACGTAG GTGGTTTGGA GAGACGTCTG CGACTGTAGT AAAAAAGATT AGGAATGCTC GGAGTGAGGG CCTGAAGATT GTTGTGTGCA TTGGGGAAGA CGAGAAATGC AGGGAGGACG GAAGGCACTT GGATGTAATA AAGGACCAGT TACTGTCTCT GGGAGAGGAG ATTGGGGGAG AAGGCATTGA CATTGCATAT GAGCCTGTAT GGGCTATAGG AACGGGGAAG GTTCCCACCA ATGATCAGAT CAGAGAGGTG GTTTCTGGGA TTAAGAGATG GGGTACGGGC ATGGGGTTTG TTGGGCGTGT GATATATGGG GGGTCTGTCT CGATGTTGAA CTGCCAAACT ATCCTCGATG TAGAGGAAAT AGATGGTTTC TTGATTGGCG GAACCTCGTT GAAAATGGAC TTTTGTATGA TTTCAAGTGA GATGAGTACG AAGACG
Details for EncuA.00649.a.A1.GE27218
HARVESTED ON: 7/31/2009
SEQUENCED ON: 8/12/2009
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKGNKLLAG NWKMSSSLQT FEIIKSFRAN LFAGNDTFIA VPFPYIHVAK KMFPEYIKVG AQDCSQFKSG PYTGEVSASM VSEMGAEYVI IGHSERRRWF GETSATVVKK IRNARSEGLK IVVCIGEDEK CREDGRHLDV IKDQLLSLGE EIGGEGIDIA YEPVWAIGTG KVPTNDQIRE VVSGIKRWGT GMGFVGRVIY GGSVSMLNCQ TILDVEEIDG FLIGGTSLKM DFCMISSEMS T
Validated NT Sequence
tcgtactcat ctcacttgaa atcatacaaa agtccatttt caacgaggtt ccgccaatca agaaaccatc tatttcctct acatcgagga tagtttggca gttcaacatc gagacagacc ccccatatat cacacgccca acaaacccca tgcccgtacc ccatctctta atcccagaaa ccacctctct gatctgatca ttggtgggaa ccttccccgt tcctatagcc catacaggct catatgcaat gtcaatgcct tctcccccaa tctcctctcc cagagacagt aactggtcct ttattacatc caagtgcctt ccgtcctccc tgcatttctc gtcttcccca atgcacacaa caatcttcag gccctcactc cgagcattcc taatcttttt tactacagtc gcagacgtct ctccaaacca cctacgtctt tcagaatggc cgattatcac atactcagca cccatctcgc tcaccatgga tgcagagact tcacctgtgt acgggccact tttaaactga ctgcaatcct gcgctccaac cttgatgtac tcaggaaaca tcttcttcgc gacatgaata taaggaaacg gaacggcaat aaacgtatcg tttccggcaa agagattcgc tcggaagctt ttaattatct caaaagtttg taacgacgaa ctcatcttcc agtttcctgc aagaagtttg tttcccttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKGNKLLAG NWKMSSSLQT FEIIKSFRAN LFAGNDTFIA VPFPYIHVAK KMFPEYIKVG AQDCSQFKSG PYTGEVSASM VSEMGAEYVI IGHSERRRWF GETSATVVKK IRNARSEGLK IVVCIGEDEK CREDGRHLDV IKDQLLSLGE EIGGEGIDIA YEPVWAIGTG KVPTNDQIRE VVSGIKRWGT GMGFVGRVIY GGSVSMLNCQ TILDVEEIDG FLIGGTSLKM DFCMISSEMS TKT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagggaaac aaacttcttg caggaaactg gaagatgagt tcgtcgttac aaacttttga gataattaaa agcttccgag cgaatctctt tgccggaaac gatacgttta ttgccgttcc gtttccttat attcatgtcg cgaagaagat gtttcctgag tacatcaagg ttggagcgca ggattgcagt cagtttaaaa gtggcccgta cacaggtgaa gtctctgcat ccatggtgag cgagatgggt gctgagtatg tgataatcgg ccattctgaa agacgtaggt ggtttggaga gacgtctgcg actgtagtaa aaaagattag gaatgctcgg agtgagggcc tgaagattgt tgtgtgcatt ggggaagacg agaaatgcag ggaggacgga aggcacttgg atgtaataaa ggaccagtta ctgtctctgg gagaggagat tgggggagaa ggcattgaca ttgcatatga gcctgtatgg gctataggaa cggggaaggt tcccaccaat gatcagatca gagaggtggt ttctgggatt aagagatggg gtacgggcat ggggtttgtt gggcgtgtga tatatggggg gtctgtctcg atgttgaact gccaaactat cctcgatgta gaggaaatag atggtttctt gattggcgga acctcgttga aaatggactt ttgtatgatt tcaagtgaga tgagtacgaa gacgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for EncuA.00649.a.A1.PW27207
PURIFICATION DATe: 8/18/2009
CONCENTRATION: 30.39mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKGNKLLAG NWKMSSSLQT FEIIKSFRAN LFAGNDTFIA VPFPYIHVAK KMFPEYIKVG AQDCSQFKSG PYTGEVSASM VSEMGAEYVI IGHSERRRWF GETSATVVKK IRNARSEGLK IVVCIGEDEK CREDGRHLDV IKDQLLSLGE EIGGEGIDIA YEPVWAIGTG KVPTNDQIRE VVSGIKRWGT GMGFVGRVIY GGSVSMLNCQ TILDVEEIDG FLIGGTSLKM DFCMISSEMS T
Validated NT Sequence
tcgtactcat ctcacttgaa atcatacaaa agtccatttt caacgaggtt ccgccaatca agaaaccatc tatttcctct acatcgagga tagtttggca gttcaacatc gagacagacc ccccatatat cacacgccca acaaacccca tgcccgtacc ccatctctta atcccagaaa ccacctctct gatctgatca ttggtgggaa ccttccccgt tcctatagcc catacaggct catatgcaat gtcaatgcct tctcccccaa tctcctctcc cagagacagt aactggtcct ttattacatc caagtgcctt ccgtcctccc tgcatttctc gtcttcccca atgcacacaa caatcttcag gccctcactc cgagcattcc taatcttttt tactacagtc gcagacgtct ctccaaacca cctacgtctt tcagaatggc cgattatcac atactcagca cccatctcgc tcaccatgga tgcagagact tcacctgtgt acgggccact tttaaactga ctgcaatcct gcgctccaac cttgatgtac tcaggaaaca tcttcttcgc gacatgaata taaggaaacg gaacggcaat aaacgtatcg tttccggcaa agagattcgc tcggaagctt ttaattatct caaaagtttg taacgacgaa ctcatcttcc agtttcctgc aagaagtttg tttcccttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKGNKLLAG NWKMSSSLQT FEIIKSFRAN LFAGNDTFIA VPFPYIHVAK KMFPEYIKVG AQDCSQFKSG PYTGEVSASM VSEMGAEYVI IGHSERRRWF GETSATVVKK IRNARSEGLK IVVCIGEDEK CREDGRHLDV IKDQLLSLGE EIGGEGIDIA YEPVWAIGTG KVPTNDQIRE VVSGIKRWGT GMGFVGRVIY GGSVSMLNCQ TILDVEEIDG FLIGGTSLKM DFCMISSEMS TKT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAGGGAAAC AAACTTCTTG CAGGAAACTG GAAGATGAGT TCGTCGTTAC AAACTTTTGA GATAATTAAA AGCTTCCGAG CGAATCTCTT TGCCGGAAAC GATACGTTTA TTGCCGTTCC GTTTCCTTAT ATTCATGTCG CGAAGAAGAT GTTTCCTGAG TACATCAAGG TTGGAGCGCA GGATTGCAGT CAGTTTAAAA GTGGCCCGTA CACAGGTGAA GTCTCTGCAT CCATGGTGAG CGAGATGGGT GCTGAGTATG TGATAATCGG CCATTCTGAA AGACGTAGGT GGTTTGGAGA GACGTCTGCG ACTGTAGTAA AAAAGATTAG GAATGCTCGG AGTGAGGGCC TGAAGATTGT TGTGTGCATT GGGGAAGACG AGAAATGCAG GGAGGACGGA AGGCACTTGG ATGTAATAAA GGACCAGTTA CTGTCTCTGG GAGAGGAGAT TGGGGGAGAA GGCATTGACA TTGCATATGA GCCTGTATGG GCTATAGGAA CGGGGAAGGT TCCCACCAAT GATCAGATCA GAGAGGTGGT TTCTGGGATT AAGAGATGGG GTACGGGCAT GGGGTTTGTT GGGCGTGTGA TATATGGGGG GTCTGTCTCG ATGTTGAACT GCCAAACTAT CCTCGATGTA GAGGAAATAG ATGGTTTCTT GATTGGCGGA ACCTCGTTGA AAATGGACTT TTGTATGATT TCAAGTGAGA TGAGTACGAA GACGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA