SSGCID: Target Page

EncuA.00627.a

Superoxide dismutase

CENTER ID:	EncuA.00627.a
ORGANISM:	Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE:	Microsporidiosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EncuA.00627.a.A1.GE27215	Full length( EncuA.00627.a )		1	233

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	MicrosporidiaDB:ECU11_1080
RefSeq:	NP_586414.1
UniProt:	Q8SQV7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVRNVVPMEF KLPELDYPYD ALEPIIDEET MRTHHSKHHQ AYINSLEKTL RSNSIKGKSL YYYVTKGRGI RGVKSSARRD LLNFSGGHYN HSLFWKMMCP PGTSGPMSPR LLEYIERSFG SQEKMVKEFS NAAVSLFGSG WVWLCYRQSE GILVIRKTYN QDAICMKTSS TVPILGLDVW EHAYYLKYKN VRSEYVANWW KVVNWGLVSR LFEEIALENK KLCVMSDGSI KFD

NT Sequence

ATGGTCCGAA ATGTAGTACC CATGGAATTC AAACTACCCG AACTCGACTA TCCATACGAT GCACTTGAGC CGATTATCGA CGAGGAGACA ATGAGGACGC ACCACTCCAA GCACCACCAG GCTTACATAA ATAGCCTCGA GAAGACACTC AGGAGTAACA GCATCAAGGG CAAGAGCCTC TACTACTACG TGACCAAGGG CCGGGGGATC AGGGGAGTCA AGAGCAGTGC AAGGAGAGAC CTGCTCAACT TCTCTGGAGG GCACTACAAC CACAGTCTGT TCTGGAAGAT GATGTGTCCC CCAGGAACCA GCGGGCCTAT GAGCCCAAGG CTTTTGGAGT ACATAGAGAG ATCCTTTGGA TCTCAGGAGA AGATGGTCAA GGAGTTTAGT AATGCGGCAG TGTCGCTATT TGGCTCCGGA TGGGTGTGGC TATGCTATAG ACAGAGCGAG GGCATTCTTG TGATAAGGAA GACATACAAC CAGGATGCTA TATGCATGAA GACCTCCAGC ACCGTTCCGA TACTTGGACT GGATGTCTGG GAGCATGCAT ATTACCTGAA GTACAAGAAT GTGAGATCTG AGTATGTAGC AAACTGGTGG AAGGTTGTAA ACTGGGGCCT GGTTAGTAGA CTGTTTGAGG AGATAGCACT AGAAAACAAG AAGCTGTGTG TTATGTCTGA CGGTTCTATT AAATTTGAT

Details for EncuA.00627.a.A1.GE27215

HARVESTED ON:	7/31/2009
SEQUENCED ON:	8/12/2009
EXPECTED MW:	29kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	91

Validated AA Sequence

Validated NT Sequence

taatcaaatt taatagaacc gtcagacata acacacagct tcttgttttc tagtgctatc tcctcaaaca gtctactaac caggccccag tttacaacct tccaccagtt tgctacatac tcagatctca cattcttgta cttcaggtaa tatgcatgct cccagacatc cagtccaagt atcggaacgg tgctggaggt cttcatgcat atagcatcct ggttgtatgt cttccttatc acaagaatgc cctcgctctg tctatagcat agccacaccc atccggagcc aaatagcgac actgccgcat tactaaactc cttgaccatc ttctcctgag atccaaagga tctctctatg tactccaaaa gccttgggct cataggcccg ctggttcctg ggggacacat catcttccag aacagactgt ggttgtagtg ccctccagag aagttgagca ggtctctcct tgcactgctc ttgactcccc tgatcccccg gcccttggtc acgtagtagt agaggctctt gcccttgatg ctgttactcc tgagtgtctt ctcgaggcta tttatgtaag cctggtggtg cttggagtgg tgcgtcctca ttgtctcctc gtcgataatc ggctcaagtg catcgtatgg atagtcgagt tcgggtagtt tgaattccat gggtactaca tttcggacca tcgaaccagg accctgggtc tgagnttcc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVRNVVPME FKLPELDYPY DALEPIIDEE TMRTHHSKHH QAYINSLEKT LRSNSIKGKS LYYYVTKGRG IRGVKSSARR DLLNFSGGHY NHSLFWKMMC PPGTSGPMSP RLLEYIERSF GSQEKMVKEF SNAAVSLFGS GWVWLCYRQS EGILVIRKTY NQDAICMKTS STVPILGLDV WEHAYYLKYK NVRSEYVANW WKVVNWGLVS RLFEEIALEN KKLCVMSDGS IKFD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtccgaaat gtagtaccca tggaattcaa actacccgaa ctcgactatc catacgatgc acttgagccg attatcgacg aggagacaat gaggacgcac cactccaagc accaccaggc ttacataaat agcctcgaga agacactcag gagtaacagc atcaagggca agagcctcta ctactacgtg accaagggcc gggggatcag gggagtcaag agcagtgcaa ggagagacct gctcaacttc tctggagggc actacaacca cagtctgttc tggaagatga tgtgtccccc aggaaccagc gggcctatga gcccaaggct tttggagtac atagagagat cctttggatc tcaggagaag atggtcaagg agtttagtaa tgcggcagtg tcgctatttg gctccggatg ggtgtggcta tgctatagac agagcgaggg cattcttgtg ataaggaaga catacaacca ggatgctata tgcatgaaga cctccagcac cgttccgata cttggactgg atgtctggga gcatgcatat tacctgaagt acaagaatgt gagatctgag tatgtagcaa actggtggaa ggttgtaaac tggggcctgg ttagtagact gtttgaggag atagcactag aaaacaagaa gctgtgtgtt atgtctgacg gttctattaa atttgataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa