EncuA.00612.a

Lysyl-tRNA synthetase

CENTER ID: EncuA.00612.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00612.a.A1.GE27216 Full length( EncuA.00612.a ) 1 440
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU04_0580
RefSeq: NP_584742.1
UniProt: Q8SS56

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSMRLHARFC FFVVTSNGES LQVVVDTKEA GREQMAKFLR RGDVVGFTGN PGRTRTLEAS VFATDIIVLT PCLRTIPTEH FGLKDPETIY RKRYMDLLIN RESRNRFQKR AQIIGYIRSF LDSRGFLEVE TPMMNLIPGG AAAKPFITHH NELKLDLYMR VSPELYLKKL VVGGLERVYE IGKQFRNEGI DLTHNPEFTS CEFYMAYADY NDLMEMTEEL ISGMVENMFG TDTIVYSPKK REERTESVEL SFKRPFRVIS ILEELNARLG LDLSGETLDR EETLEKLLSA CDKEGLSVEK PRTLSRVLDK LIGHVIEPQC VNPTFVKDHP IAMSPLAKNH RSKAGLTERF ELFINCKEIC NAYTELNNPF EQRERFLQQT QDLNAGDDEA MMNDEDFCTA LEYGLPPTGG WGIGIDRLVM YLTDAANIRD VIFFPTMKPE
NT Sequence
ATGAGCATGA GGCTCCATGC AAGGTTTTGC TTTTTTGTTG TTACCTCGAA CGGGGAGAGT CTCCAGGTTG TTGTGGACAC CAAGGAGGCG GGGAGAGAGC AGATGGCCAA GTTCCTCAGG AGAGGAGATG TTGTTGGGTT TACCGGGAAC CCTGGAAGGA CGAGAACACT GGAGGCGTCT GTATTTGCCA CGGATATAAT TGTCCTTACG CCGTGCCTCA GGACTATTCC TACGGAGCAC TTTGGGCTGA AGGACCCCGA GACCATCTAC AGGAAAAGAT ATATGGATCT TTTGATTAAC AGGGAATCAA GGAACAGGTT CCAGAAGAGA GCGCAGATCA TTGGATACAT TAGGAGCTTT TTGGATTCGA GAGGGTTTCT GGAGGTTGAA ACGCCGATGA TGAACCTGAT TCCTGGAGGA GCAGCGGCAA AGCCCTTTAT AACCCACCAC AACGAGCTCA AGCTGGATCT GTACATGAGG GTATCTCCTG AGCTGTACCT GAAGAAGCTT GTTGTTGGGG GTCTTGAGCG GGTGTATGAG ATAGGGAAGC AGTTTAGGAA CGAAGGGATT GATCTTACCC ACAACCCGGA GTTCACGTCT TGCGAGTTCT ACATGGCGTA TGCAGACTAC AACGACCTTA TGGAGATGAC TGAGGAGCTG ATAAGTGGAA TGGTTGAAAA CATGTTTGGT ACGGACACGA TAGTGTACAG CCCGAAGAAG AGAGAGGAAA GGACGGAGTC TGTGGAGCTG TCGTTCAAGA GGCCCTTCCG AGTCATAAGT ATTCTCGAGG AGCTTAATGC TAGACTCGGG CTGGATCTCA GTGGGGAGAC TCTTGACAGG GAGGAGACTC TGGAGAAGCT CTTGTCTGCC TGTGATAAGG AGGGGCTATC TGTTGAAAAG CCTCGGACTC TATCACGGGT ACTTGACAAG CTGATTGGAC ATGTTATTGA GCCGCAGTGT GTGAACCCAA CCTTTGTCAA GGACCACCCA ATAGCAATGT CGCCGCTGGC CAAGAACCAT AGGTCGAAGG CCGGGTTGAC TGAGAGGTTT GAGCTCTTTA TCAACTGCAA GGAGATATGT AATGCCTATA CAGAGCTGAA CAACCCGTTT GAGCAACGAG AAAGGTTCCT CCAGCAGACC CAGGACCTGA ATGCTGGAGA TGATGAAGCA ATGATGAATG ATGAGGACTT TTGCACAGCC CTTGAGTATG GACTGCCCCC CACTGGGGGA TGGGGGATAG GAATTGACAG GCTTGTGATG TATCTGACCG ATGCGGCGAA TATCCGGGAC GTGATCTTTT TCCCCACGAT GAAGCCTGAA
Details for EncuA.00612.a.A1.GE27216
HARVESTED ON: 7/31/2009
SEQUENCED ON: 8/12/2009
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MSMRLHARFC FFVVTSNGES LQVVVDTKEA GREQMAKFLR RGDVVGFTGN PGRTRTLEAS VFATDIIVLT PCLRTIPTEH FGLKDPETIY RKRYMDLLIN RESRNRFQKR AQIIGYIRSF LDSRGFLEVE TPMMNLIPGG AAAKPFMTHH NELKLDLYMR VSPELYLKKL VVGGLERVYE IGKQFRNEGI DLTHNPEFTS CEFYMAYADY NDLMEMTEEL ISGMVENMFG TDTIVYSPKK REERTESVEL SFKRPFRVIS ILEELNARLG LDLSGETLDR EETLEKLLSA CDKEGLSVEK PRTLSRVLDK LIGHVIEPQC VNPTFVKDHP IAMSPLAKNH RSKAGLTERF ELFINCKEIC NAYTELNNPF EQRERFLQQT QDLNAGDDEA MMNDEDFCTA LEYGLPPTGG WGIGIDRLVM YLTDAANIRD VIFFPTMKPE
Validated NT Sequence
gggtaccctg gaagctcaga cccagggtcc tggttcgatg agcatgaggc tccatgcaag gttttgcttt tttgttgtta cctcgaacgg ggagagtctc caggttgttg tggacaccaa ggaggcgggg agagagcaga tggccaagtt cctcaggaga ggagatgttg ttgggtttac cgggaaccct ggaaggacga gaacactgga ggcgtctgta tttgccacgg atataattgt ccttacgccg tgcctcagga ctattcctac ggagcacttt gggctgaagg accccgagac catctacagg aaaagatata tggatctttt gattaacagg gaatcaagga acaggttcca gaagagagcg cagatcattg gatacattag gagctttttg gattcgagag ggtttctgga ggttgaaacg ccgatgatga acctgattcc tggaggagca gcggcaaagc cctttatgac ccaccacaac gagctcaagc tggatctgta catgagggta tctcctgagc tgtacctgaa gaagcttgtt gttgggggtc ttgagcgggt gtatgagata gggaagcagt ttaggaacga agggattgat cttacccaca acccggagtt cacgtcttgc gagttctaca tggcgtatgc agactacaac gaccttatgg agatgactga ggagctgata agtggaatgg ttgaaaacat gtttggtacg gacacgatag tgtacagccc gaagaagaga gaggaaagga cggagtctgt ggagctgtcg ttcaagaggc ccttccgagt cataagtatt ctcgaggagc ttaatgctag actcgggctg gatctcagtg gggagactct tgacagggag gagactctgg agaagctctt gtctgcctgt gataaggagg ggctatctgt tgaaaagcct cggactctat cacgggtact tgacaagctg attggacatg ttattgagcc gcagtgtgtg aacccaacct ttgtcaagga ccacccaata gcaatgtcgc cgctggccaa gaaccatagg tcgaaggccg ggttgactga gaggtttgag ctctttatca actgcaagga gatatgtaat gcctatacag agctgaacaa cccgtttgag caacgagaaa ggttcctcca gcagacccag gacctgaatg ctggagatga tgaagcaatg atgaatgatg aggacttttg cacagccctt gagtatggac tgccccccac tgggggatgg gggataggaa ttgacaggct tgtgatgtat ctgaccgatg cggcgaatat ccgggacgtg atctttttcc ccacgatgaa gcctgaataa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSMRLHARF CFFVVTSNGE SLQVVVDTKE AGREQMAKFL RRGDVVGFTG NPGRTRTLEA SVFATDIIVL TPCLRTIPTE HFGLKDPETI YRKRYMDLLI NRESRNRFQK RAQIIGYIRS FLDSRGFLEV ETPMMNLIPG GAAAKPFITH HNELKLDLYM RVSPELYLKK LVVGGLERVY EIGKQFRNEG IDLTHNPEFT SCEFYMAYAD YNDLMEMTEE LISGMVENMF GTDTIVYSPK KREERTESVE LSFKRPFRVI SILEELNARL GLDLSGETLD REETLEKLLS ACDKEGLSVE KPRTLSRVLD KLIGHVIEPQ CVNPTFVKDH PIAMSPLAKN HRSKAGLTER FELFINCKEI CNAYTELNNP FEQRERFLQQ TQDLNAGDDE AMMNDEDFCT ALEYGLPPTG GWGIGIDRLV MYLTDAANIR DVIFFPTMKP E
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcatgagg ctccatgcaa ggttttgctt ttttgttgtt acctcgaacg gggagagtct ccaggttgtt gtggacacca aggaggcggg gagagagcag atggccaagt tcctcaggag aggagatgtt gttgggttta ccgggaaccc tggaaggacg agaacactgg aggcgtctgt atttgccacg gatataattg tccttacgcc gtgcctcagg actattccta cggagcactt tgggctgaag gaccccgaga ccatctacag gaaaagatat atggatcttt tgattaacag ggaatcaagg aacaggttcc agaagagagc gcagatcatt ggatacatta ggagcttttt ggattcgaga gggtttctgg aggttgaaac gccgatgatg aacctgattc ctggaggagc agcggcaaag ccctttataa cccaccacaa cgagctcaag ctggatctgt acatgagggt atctcctgag ctgtacctga agaagcttgt tgttgggggt cttgagcggg tgtatgagat agggaagcag tttaggaacg aagggattga tcttacccac aacccggagt tcacgtcttg cgagttctac atggcgtatg cagactacaa cgaccttatg gagatgactg aggagctgat aagtggaatg gttgaaaaca tgtttggtac ggacacgata gtgtacagcc cgaagaagag agaggaaagg acggagtctg tggagctgtc gttcaagagg cccttccgag tcataagtat tctcgaggag cttaatgcta gactcgggct ggatctcagt ggggagactc ttgacaggga ggagactctg gagaagctct tgtctgcctg tgataaggag gggctatctg ttgaaaagcc tcggactcta tcacgggtac ttgacaagct gattggacat gttattgagc cgcagtgtgt gaacccaacc tttgtcaagg accacccaat agcaatgtcg ccgctggcca agaaccatag gtcgaaggcc gggttgactg agaggtttga gctctttatc aactgcaagg agatatgtaa tgcctataca gagctgaaca acccgtttga gcaacgagaa aggttcctcc agcagaccca ggacctgaat gctggagatg atgaagcaat gatgaatgat gaggactttt gcacagccct tgagtatgga ctgcccccca ctgggggatg ggggatagga attgacaggc ttgtgatgta tctgaccgat gcggcgaata tccgggacgt gatctttttc cccacgatga agcctgaaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa