EncuA.00600.a
Tryptophanyl-tRNA synthetase
| CENTER ID: | EncuA.00600.a |
| ORGANISM: | Encephalitozoon cuniculi GB-M1 |
| ASSOCIATED DISEASE: | Microsporidiosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EncuA.00600.a.A1.GE27214 | Full length( EncuA.00600.a ) | 1 | 385 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EncuA.00600.a.A1.PS00344 | Full length( EncuA.00600.a ) | 1 | 385 |
Structures
3TZE
DEPOSITED: 9/27/2011
DETERMINATION: XRay
CLONE: EncuA.00600.a.A1.GE27214
PROTEIN: EncuA.00600.a.A1.PS00344
Publications by SSGCID
Ligand co-crystallization of aminoacyl-tRNA synthetases from infectious disease organisms
Clifton MC, Dranow DM, Edwards TE, Lorimer DD, Manoil C, Moen S, Myler PJ, Sankaran B, Sharma A, Staker BL, Van Voorhis WC
Sci Rep - 2017
volume 7, issue 1, pages 223
PMID: 28303005; PMCID: PMC5428304
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | MicrosporidiaDB:ECU11_0530 |
| OrthoMCL: | OG5_127096 |
| RefSeq: | NP_586359.1 |
| UniProt: | O96771 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEQRITPWD VEVVSTDEVP VAIDYDKIIN QFGCEKFNQA LADRLEKLSG KPAHYFFRRG IVFAHRDFNL LLDEIANNRP FYLYTGRGPS SKTMHIGHTI PFLLCKYMQD AFKIRLVIQI TDDEKFLWKS MRLEDAMAYG RENIKDIVAL GFDPKLTYIF SNVEASHHFE ENILKISKTI NLNEAIKVFG FDMSSNIGQV GFPAKEIAPC FSSSFRFIGK GAMCLVPAAV DQDPFFRLAR DKAKALGEKK PSSIYVSLLP DLKGVNRKMS ASDPNSSIYL DDAQDTIRKK IIAYAYSGGR KTLEEHREKG GDIDVDVPFE YLKYFLDDDQ ELEKYRSGYI KGEITSKEMK EKCVVVIQEF VSRYQESRKR VTDDDLRAFI DINKF
NT Sequence
ATGGCAGAGC AGAGAATCAC GCCGTGGGAC GTCGAGGTTG TGTCTACAGA CGAAGTGCCT GTGGCCATTG ATTACGACAA GATAATCAAC CAGTTTGGGT GTGAGAAGTT TAACCAGGCC CTTGCGGACC GTTTGGAAAA GCTATCAGGC AAGCCGGCAC ACTATTTTTT TAGAAGAGGC ATAGTCTTTG CACATAGAGA CTTCAACCTT CTTCTTGACG AGATAGCCAA CAACAGGCCG TTCTATCTGT ACACCGGAAG AGGCCCCAGC TCGAAAACCA TGCATATCGG GCACACCATT CCCTTTCTTC TATGCAAGTA TATGCAGGAT GCCTTCAAGA TACGCCTTGT GATCCAGATA ACGGACGACG AAAAGTTCCT GTGGAAAAGC ATGAGGCTCG AGGACGCCAT GGCATATGGA AGAGAAAACA TCAAGGACAT TGTGGCACTT GGGTTTGATC CAAAGCTGAC ATACATATTC TCCAATGTCG AGGCCTCCCA CCACTTCGAG GAAAACATTT TGAAGATATC AAAGACAATC AATCTCAACG AGGCGATCAA GGTCTTTGGA TTCGATATGT CGAGCAACAT AGGACAGGTG GGGTTTCCGG CAAAGGAAAT AGCGCCCTGC TTCTCAAGCT CCTTCAGATT CATCGGAAAG GGGGCCATGT GCCTCGTTCC TGCAGCAGTC GATCAGGACC CGTTCTTTAG GCTGGCAAGA GACAAAGCAA AGGCGCTAGG AGAGAAGAAG CCCTCGTCCA TATATGTATC ACTCCTCCCG GATCTTAAGG GAGTGAACAG AAAGATGTCT GCCAGCGATC CAAACAGTTC CATATATCTG GATGATGCCC AGGATACAAT AAGGAAGAAA ATCATCGCAT ATGCCTATTC GGGAGGCAGG AAGACGCTGG AGGAGCACAG AGAAAAGGGG GGAGACATTG ACGTGGATGT CCCGTTTGAA TATCTGAAAT ACTTCCTGGA CGATGACCAG GAGCTTGAGA AGTATCGCTC GGGATACATC AAGGGAGAGA TTACTAGTAA GGAAATGAAG GAAAAATGCG TTGTTGTAAT ACAGGAGTTT GTGTCGAGGT ACCAAGAGTC CAGGAAAAGA GTCACCGACG ACGATTTGAG GGCATTCATC GATATCAATA AATTC
Details for EncuA.00600.a.A1.GE27214
| HARVESTED ON: | 7/31/2009 |
| SEQUENCED ON: | 8/12/2009 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 47kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMAEQRIT PWDVEVVSTD EVPVAIDYDK IINQFGCEKF NQALADRLEK LSGKPAHYFF RRGIVFAHRD FNLLLDEIAN NRPFYLYTGR GPSSKTMHIG HTIPFLLCKY MQDAFKIRLV IQITDDEKFL WKSMRLEDAM AYGRENIKDI VTLGFDPKLT YIFSNVEASH HFEENILKIS KTINLNEAIK VFGFDMSSNI GQVGFPAKEI APCFSSSFRF IGKGAMCLVP AAVDQDPFFR LARDKAKALG EKKPSSIYVS LLPDLKGVNR KMSASDPNSS IYLDDAQDTI RKKIIAYAYS GGRKTLEEHR EKGGDIDVDV PFEYLKYFLD DDQELEKYRS GYIKGEITSK EMKEKCVVVI QEFVSRYQES RKRVTDDDLR AFIDIN
Validated NT Sequence
ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcaga gcagagaatc acgccgtggg acgtcgaggt tgtgtctaca gacgaagtgc ctgtggccat tgattacgac aagataatca accagtttgg gtgtgagaag tttaaccagg cccttgcgga ccgtttggaa aagctatcag gcaagccggc acactatttt tttagaagag gcatagtctt tgcacataga gacttcaacc ttcttcttga cgagatagcc aacaacaggc cgttctatct gtacaccgga agaggcccca gctcgaaaac catgcatatc gggcacacca ttccctttct tctatgcaag tatatgcagg atgccttcaa gatacgcctt gtgatccaga taacggacga cgaaaagttc ctgtggaaaa gcatgaggct cgaggacgcc atggcatatg gaagagaaaa catcaaggac attgtgacac ttgggtttga tccaaagctg acatacatat tctccaatgt cgaggcctcc caccacttcg aggaaaacat tttgaagata tcaaagacaa tcaatctcaa cgaggcgatc aaggtctttg gattcgatat gtcgagcaac ataggacagg tggggtttcc ggcaaaggaa atagcgccct gcttctcaag ctccttcaga ttcatcggaa agggggccat gtgcctcgtt cctgcagcag tcgatcagga cccgttcttt aggctggcaa gagacaaagc aaaggcgcta ggagagaaga agccctcgtc catatatgta tcactcctcc cggatcttaa gggagtgaac agaaagatgt ctgccagcga tccaaacagt tccatatatc tggatgatgc ccaggataca ataaggaaga aaatcatcgc atatgcctat tcgggaggca ggaagacgct ggaggagcac agagaaaagg ggggagacat tgacgtggat gtcccgtttg aatatctgaa atacttcctg gacgatgacc aggagcttga gaagtatcgc tcgggataca tcaagggaga gattactagt aaggaaatga aggaaaaatg cgttgttgta atacaggagt ttgtgtcgag gtaccaagag tccaggaaaa gagtcaccga cgacgatttg agggcattca tcgatatcaa ta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEQRITPW DVEVVSTDEV PVAIDYDKII NQFGCEKFNQ ALADRLEKLS GKPAHYFFRR GIVFAHRDFN LLLDEIANNR PFYLYTGRGP SSKTMHIGHT IPFLLCKYMQ DAFKIRLVIQ ITDDEKFLWK SMRLEDAMAY GRENIKDIVA LGFDPKLTYI FSNVEASHHF EENILKISKT INLNEAIKVF GFDMSSNIGQ VGFPAKEIAP CFSSSFRFIG KGAMCLVPAA VDQDPFFRLA RDKAKALGEK KPSSIYVSLL PDLKGVNRKM SASDPNSSIY LDDAQDTIRK KIIAYAYSGG RKTLEEHREK GGDIDVDVPF EYLKYFLDDD QELEKYRSGY IKGEITSKEM KEKCVVVIQE FVSRYQESRK RVTDDDLRAF IDINKF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagagcag agaatcacgc cgtgggacgt cgaggttgtg tctacagacg aagtgcctgt ggccattgat tacgacaaga taatcaacca gtttgggtgt gagaagttta accaggccct tgcggaccgt ttggaaaagc tatcaggcaa gccggcacac tattttttta gaagaggcat agtctttgca catagagact tcaaccttct tcttgacgag atagccaaca acaggccgtt ctatctgtac accggaagag gccccagctc gaaaaccatg catatcgggc acaccattcc ctttcttcta tgcaagtata tgcaggatgc cttcaagata cgccttgtga tccagataac ggacgacgaa aagttcctgt ggaaaagcat gaggctcgag gacgccatgg catatggaag agaaaacatc aaggacattg tggcacttgg gtttgatcca aagctgacat acatattctc caatgtcgag gcctcccacc acttcgagga aaacattttg aagatatcaa agacaatcaa tctcaacgag gcgatcaagg tctttggatt cgatatgtcg agcaacatag gacaggtggg gtttccggca aaggaaatag cgccctgctt ctcaagctcc ttcagattca tcggaaaggg ggccatgtgc ctcgttcctg cagcagtcga tcaggacccg ttctttaggc tggcaagaga caaagcaaag gcgctaggag agaagaagcc ctcgtccata tatgtatcac tcctcccgga tcttaaggga gtgaacagaa agatgtctgc cagcgatcca aacagttcca tatatctgga tgatgcccag gatacaataa ggaagaaaat catcgcatat gcctattcgg gaggcaggaa gacgctggag gagcacagag aaaagggggg agacattgac gtggatgtcc cgtttgaata tctgaaatac ttcctggacg atgaccagga gcttgagaag tatcgctcgg gatacatcaa gggagagatt actagtaagg aaatgaagga aaaatgcgtt gttgtaatac aggagtttgt gtcgaggtac caagagtcca ggaaaagagt caccgacgac gatttgaggg cattcatcga tatcaataaa ttcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for EncuA.00600.a.A1.PS00344
| PURIFICATION DATe: | 9/4/2009 |
| CONCENTRATION: | 23.46mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMAEQRIT PWDVEVVSTD EVPVAIDYDK IINQFGCEKF NQALADRLEK LSGKPAHYFF RRGIVFAHRD FNLLLDEIAN NRPFYLYTGR GPSSKTMHIG HTIPFLLCKY MQDAFKIRLV IQITDDEKFL WKSMRLEDAM AYGRENIKDI VTLGFDPKLT YIFSNVEASH HFEENILKIS KTINLNEAIK VFGFDMSSNI GQVGFPAKEI APCFSSSFRF IGKGAMCLVP AAVDQDPFFR LARDKAKALG EKKPSSIYVS LLPDLKGVNR KMSASDPNSS IYLDDAQDTI RKKIIAYAYS GGRKTLEEHR EKGGDIDVDV PFEYLKYFLD DDQELEKYRS GYIKGEITSK EMKEKCVVVI QEFVSRYQES RKRVTDDDLR AFIDIN
Validated NT Sequence
ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcaga gcagagaatc acgccgtggg acgtcgaggt tgtgtctaca gacgaagtgc ctgtggccat tgattacgac aagataatca accagtttgg gtgtgagaag tttaaccagg cccttgcgga ccgtttggaa aagctatcag gcaagccggc acactatttt tttagaagag gcatagtctt tgcacataga gacttcaacc ttcttcttga cgagatagcc aacaacaggc cgttctatct gtacaccgga agaggcccca gctcgaaaac catgcatatc gggcacacca ttccctttct tctatgcaag tatatgcagg atgccttcaa gatacgcctt gtgatccaga taacggacga cgaaaagttc ctgtggaaaa gcatgaggct cgaggacgcc atggcatatg gaagagaaaa catcaaggac attgtgacac ttgggtttga tccaaagctg acatacatat tctccaatgt cgaggcctcc caccacttcg aggaaaacat tttgaagata tcaaagacaa tcaatctcaa cgaggcgatc aaggtctttg gattcgatat gtcgagcaac ataggacagg tggggtttcc ggcaaaggaa atagcgccct gcttctcaag ctccttcaga ttcatcggaa agggggccat gtgcctcgtt cctgcagcag tcgatcagga cccgttcttt aggctggcaa gagacaaagc aaaggcgcta ggagagaaga agccctcgtc catatatgta tcactcctcc cggatcttaa gggagtgaac agaaagatgt ctgccagcga tccaaacagt tccatatatc tggatgatgc ccaggataca ataaggaaga aaatcatcgc atatgcctat tcgggaggca ggaagacgct ggaggagcac agagaaaagg ggggagacat tgacgtggat gtcccgtttg aatatctgaa atacttcctg gacgatgacc aggagcttga gaagtatcgc tcgggataca tcaagggaga gattactagt aaggaaatga aggaaaaatg cgttgttgta atacaggagt ttgtgtcgag gtaccaagag tccaggaaaa gagtcaccga cgacgatttg agggcattca tcgatatcaa ta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEQRITPW DVEVVSTDEV PVAIDYDKII NQFGCEKFNQ ALADRLEKLS GKPAHYFFRR GIVFAHRDFN LLLDEIANNR PFYLYTGRGP SSKTMHIGHT IPFLLCKYMQ DAFKIRLVIQ ITDDEKFLWK SMRLEDAMAY GRENIKDIVA LGFDPKLTYI FSNVEASHHF EENILKISKT INLNEAIKVF GFDMSSNIGQ VGFPAKEIAP CFSSSFRFIG KGAMCLVPAA VDQDPFFRLA RDKAKALGEK KPSSIYVSLL PDLKGVNRKM SASDPNSSIY LDDAQDTIRK KIIAYAYSGG RKTLEEHREK GGDIDVDVPF EYLKYFLDDD QELEKYRSGY IKGEITSKEM KEKCVVVIQE FVSRYQESRK RVTDDDLRAF IDINKF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCAGAGCAG AGAATCACGC CGTGGGACGT CGAGGTTGTG TCTACAGACG AAGTGCCTGT GGCCATTGAT TACGACAAGA TAATCAACCA GTTTGGGTGT GAGAAGTTTA ACCAGGCCCT TGCGGACCGT TTGGAAAAGC TATCAGGCAA GCCGGCACAC TATTTTTTTA GAAGAGGCAT AGTCTTTGCA CATAGAGACT TCAACCTTCT TCTTGACGAG ATAGCCAACA ACAGGCCGTT CTATCTGTAC ACCGGAAGAG GCCCCAGCTC GAAAACCATG CATATCGGGC ACACCATTCC CTTTCTTCTA TGCAAGTATA TGCAGGATGC CTTCAAGATA CGCCTTGTGA TCCAGATAAC GGACGACGAA AAGTTCCTGT GGAAAAGCAT GAGGCTCGAG GACGCCATGG CATATGGAAG AGAAAACATC AAGGACATTG TGGCACTTGG GTTTGATCCA AAGCTGACAT ACATATTCTC CAATGTCGAG GCCTCCCACC ACTTCGAGGA AAACATTTTG AAGATATCAA AGACAATCAA TCTCAACGAG GCGATCAAGG TCTTTGGATT CGATATGTCG AGCAACATAG GACAGGTGGG GTTTCCGGCA AAGGAAATAG CGCCCTGCTT CTCAAGCTCC TTCAGATTCA TCGGAAAGGG GGCCATGTGC CTCGTTCCTG CAGCAGTCGA TCAGGACCCG TTCTTTAGGC TGGCAAGAGA CAAAGCAAAG GCGCTAGGAG AGAAGAAGCC CTCGTCCATA TATGTATCAC TCCTCCCGGA TCTTAAGGGA GTGAACAGAA AGATGTCTGC CAGCGATCCA AACAGTTCCA TATATCTGGA TGATGCCCAG GATACAATAA GGAAGAAAAT CATCGCATAT GCCTATTCGG GAGGCAGGAA GACGCTGGAG GAGCACAGAG AAAAGGGGGG AGACATTGAC GTGGATGTCC CGTTTGAATA TCTGAAATAC TTCCTGGACG ATGACCAGGA GCTTGAGAAG TATCGCTCGG GATACATCAA GGGAGAGATT ACTAGTAAGG AAATGAAGGA AAAATGCGTT GTTGTAATAC AGGAGTTTGT GTCGAGGTAC CAAGAGTCCA GGAAAAGAGT CACCGACGAC GATTTGAGGG CATTCATCGA TATCAATAAA TTCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA