EncuA.00570.a

RAS-LIKE GTP-BINDING PROTEIN

CENTER ID: EncuA.00570.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00570.a.A1.GE27213 Full length( EncuA.00570.a ) 1 212
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00570.a.A1.PS00346 Full length( EncuA.00570.a ) 1 212

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU02_0410
RefSeq: NP_584568.1
UniProt: Q8SSH9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAETATADVS KQLTIIGSAT CGKTSILTRH VKRTFGTEIS PTVFNSVSNT YKHKDHVIEL RLWDTAGQDE YARFRHLALP SADYIMIVYA VDDRSSYAEV KNSLVGQVRE KAPQAKYFLV GAKTDLREDS ARLEELARNG DSPITTSEGL ELSQSIGAMG YFECSAKNNV GIDEIFEKIS EYALRAQQKE SMSFIGAMCA SLWNCLSCRQ IN
NT Sequence
ATGGCGGAGA CAGCCACAGC AGACGTATCC AAGCAGCTGA CCATCATCGG CTCTGCAACG TGTGGAAAAA CAAGCATCCT TACCAGGCAT GTAAAAAGGA CGTTTGGGAC GGAGATCAGC CCCACCGTAT TCAACTCTGT CAGCAACACA TATAAGCACA AGGACCATGT AATTGAGCTG AGGCTCTGGG ACACAGCAGG GCAGGACGAG TATGCCAGGT TCCGACACCT TGCATTGCCT TCGGCGGACT ATATTATGAT CGTGTATGCT GTTGACGACA GATCCTCCTA TGCCGAGGTT AAAAACAGCC TTGTAGGGCA AGTAAGGGAG AAGGCGCCCC AGGCCAAATA CTTCCTTGTT GGGGCGAAAA CCGATCTGAG GGAGGACAGT GCGAGACTCG AGGAACTTGC TAGGAACGGA GACTCTCCGA TAACCACTTC TGAGGGGCTG GAGCTTTCTC AGAGCATTGG GGCGATGGGA TACTTTGAGT GTTCTGCAAA GAACAATGTT GGAATCGACG AGATATTTGA GAAGATTTCT GAGTACGCTC TGAGGGCGCA GCAGAAGGAA AGCATGTCTT TCATAGGGGC GATGTGCGCT TCTCTGTGGA ACTGCCTTAG CTGCAGACAA ATAAAT
Details for EncuA.00570.a.A1.GE27213
HARVESTED ON: 7/31/2009
SEQUENCED ON: 8/12/2009
EXPECTED MW: 25kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAETATADV SKQLTIIGSA TCGKTSILTR HVKRTFGTEI SPTVFNSVSN TYKHKDHVIE LRLWDTAGQD EYARFRHLAL PSADYIMIVY AVDDRSSYAE VKNSLVGQVR EKAPQAKYFL VGAKTDLRED SARLEELARN GDSPITTSEG LELSQSIGAM GYFECSAKNN VGIDEIFEKI SEYALRAQQK ESMSFIGAMC ASLWNCLSCR QIN
Validated NT Sequence
ttattaattt atttgtctgc agctaaggca gttccacaga gaagcgcaca tcgcccctat gaaagacatg ctttccttct gctgcgccct cagagcgtac tcagaaatct tctcaaatat ctcgtcgatt ccaacattgt tctttgcaga acactcaaag tatcccatcg ccccaatgct ctgagaaagc tccagcccct cagaagtggt tatcggagag tctccgttcc tagcaagttc ctcgagtctc gcactgtcct ccctcagatc ggttttcgcc ccaacaagga agtatttggc ctggggcgcc ttctccctta cttgccctac aaggctgttt ttaacctcgg cataggagga tctgtcgtca acagcataca cgatcataat atagtccgcc gaaggcaatg caaggtgtcg gaacctggca tactcgtcct gccctgctgt gtcccagagc ctcagctcaa ttacatggtc cttgtgctta tatgtgttgc tgacagagtt gaatacggtg gggctgatct ccgtcccaaa cgtccttttt acatgcctgg taaggatgct tgtttttcca cacgttgcag agccgatgat ggtcagctgc ttggatacgt ctgctgtggc tgtctccgcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAETATADV SKQLTIIGSA TCGKTSILTR HVKRTFGTEI SPTVFNSVSN TYKHKDHVIE LRLWDTAGQD EYARFRHLAL PSADYIMIVY AVDDRSSYAE VKNSLVGQVR EKAPQAKYFL VGAKTDLRED SARLEELARN GDSPITTSEG LELSQSIGAM GYFECSAKNN VGIDEIFEKI SEYALRAQQK ESMSFIGAMC ASLWNCLSCR QIN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggagaca gccacagcag acgtatccaa gcagctgacc atcatcggct ctgcaacgtg tggaaaaaca agcatcctta ccaggcatgt aaaaaggacg tttgggacgg agatcagccc caccgtattc aactctgtca gcaacacata taagcacaag gaccatgtaa ttgagctgag gctctgggac acagcagggc aggacgagta tgccaggttc cgacaccttg cattgccttc ggcggactat attatgatcg tgtatgctgt tgacgacaga tcctcctatg ccgaggttaa aaacagcctt gtagggcaag taagggagaa ggcgccccag gccaaatact tccttgttgg ggcgaaaacc gatctgaggg aggacagtgc gagactcgag gaacttgcta ggaacggaga ctctccgata accacttctg aggggctgga gctttctcag agcattgggg cgatgggata ctttgagtgt tctgcaaaga acaatgttgg aatcgacgag atatttgaga agatttctga gtacgctctg agggcgcagc agaaggaaag catgtctttc ataggggcga tgtgcgcttc tctgtggaac tgccttagct gcagacaaat aaataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for EncuA.00570.a.A1.PS00346
PURIFICATION DATe: 9/3/2009
CONCENTRATION: 5.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAETATADV SKQLTIIGSA TCGKTSILTR HVKRTFGTEI SPTVFNSVSN TYKHKDHVIE LRLWDTAGQD EYARFRHLAL PSADYIMIVY AVDDRSSYAE VKNSLVGQVR EKAPQAKYFL VGAKTDLRED SARLEELARN GDSPITTSEG LELSQSIGAM GYFECSAKNN VGIDEIFEKI SEYALRAQQK ESMSFIGAMC ASLWNCLSCR QIN
Validated NT Sequence
ttattaattt atttgtctgc agctaaggca gttccacaga gaagcgcaca tcgcccctat gaaagacatg ctttccttct gctgcgccct cagagcgtac tcagaaatct tctcaaatat ctcgtcgatt ccaacattgt tctttgcaga acactcaaag tatcccatcg ccccaatgct ctgagaaagc tccagcccct cagaagtggt tatcggagag tctccgttcc tagcaagttc ctcgagtctc gcactgtcct ccctcagatc ggttttcgcc ccaacaagga agtatttggc ctggggcgcc ttctccctta cttgccctac aaggctgttt ttaacctcgg cataggagga tctgtcgtca acagcataca cgatcataat atagtccgcc gaaggcaatg caaggtgtcg gaacctggca tactcgtcct gccctgctgt gtcccagagc ctcagctcaa ttacatggtc cttgtgctta tatgtgttgc tgacagagtt gaatacggtg gggctgatct ccgtcccaaa cgtccttttt acatgcctgg taaggatgct tgtttttcca cacgttgcag agccgatgat ggtcagctgc ttggatacgt ctgctgtggc tgtctccgcc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAETATADV SKQLTIIGSA TCGKTSILTR HVKRTFGTEI SPTVFNSVSN TYKHKDHVIE LRLWDTAGQD EYARFRHLAL PSADYIMIVY AVDDRSSYAE VKNSLVGQVR EKAPQAKYFL VGAKTDLRED SARLEELARN GDSPITTSEG LELSQSIGAM GYFECSAKNN VGIDEIFEKI SEYALRAQQK ESMSFIGAMC ASLWNCLSCR QIN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGAGACA GCCACAGCAG ACGTATCCAA GCAGCTGACC ATCATCGGCT CTGCAACGTG TGGAAAAACA AGCATCCTTA CCAGGCATGT AAAAAGGACG TTTGGGACGG AGATCAGCCC CACCGTATTC AACTCTGTCA GCAACACATA TAAGCACAAG GACCATGTAA TTGAGCTGAG GCTCTGGGAC ACAGCAGGGC AGGACGAGTA TGCCAGGTTC CGACACCTTG CATTGCCTTC GGCGGACTAT ATTATGATCG TGTATGCTGT TGACGACAGA TCCTCCTATG CCGAGGTTAA AAACAGCCTT GTAGGGCAAG TAAGGGAGAA GGCGCCCCAG GCCAAATACT TCCTTGTTGG GGCGAAAACC GATCTGAGGG AGGACAGTGC GAGACTCGAG GAACTTGCTA GGAACGGAGA CTCTCCGATA ACCACTTCTG AGGGGCTGGA GCTTTCTCAG AGCATTGGGG CGATGGGATA CTTTGAGTGT TCTGCAAAGA ACAATGTTGG AATCGACGAG ATATTTGAGA AGATTTCTGA GTACGCTCTG AGGGCGCAGC AGAAGGAAAG CATGTCTTTC ATAGGGGCGA TGTGCGCTTC TCTGTGGAAC TGCCTTAGCT GCAGACAAAT AAATAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA