EncuA.00523.a

KINESIN-LIKE PROTEIN

CENTER ID: EncuA.00523.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00523.a.A1.GE27212 Full length( EncuA.00523.a ) 1 420
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU08_0890
OrthoMCL: OG5_130652
RefSeq: NP_597219.1
UniProt: Q8SRB7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLENVRTYLR IKPQAIPEDI VVGGDSVIVD GSSFCFDRVF RGSTQQEVFQ EISKSLLVEC MQGYNCTLFA YGQTGSGKTY TIQGNAHSIG IVQRSLSLLH RNADLRISLS FVEIYNETML DLFDPEAILS IREDPLCGVV VDGLTIVDCD SYEKSMEMYS RGTRTRRTSS TSMNKESSRS HSVFTVYLKS SGEVMSRSSR VCFVDLAGSE RLREREVEET KMKETANINK SLLYLGKVIN KLSNGEEGHI GYRESKLTFL LKDSLGGNCK LTVIGNVSLE SRSDSINTMN FLQRSKMIRN LAVVNSDVNG ELEEVKSRLK TLDSENQSLK ARIALIDSQI QEESRALPSY HYDIKRVKDA VDDIMSTVLD LGETVGRVSG VEFDKNRRLL LSIHECFMSM HRRQEDAESS TKRKRRDAKE
NT Sequence
ATGCTTGAAA ATGTCAGGAC ATATCTGAGG ATCAAGCCAC AGGCAATTCC AGAGGACATA GTTGTTGGCG GAGACTCAGT AATCGTGGAT GGAAGCAGCT TCTGCTTCGA CAGGGTTTTC AGAGGCTCCA CGCAGCAAGA GGTTTTCCAG GAGATATCAA AGAGTCTTCT GGTGGAGTGC ATGCAGGGAT ACAACTGCAC GCTGTTTGCA TATGGACAAA CAGGGAGCGG GAAGACCTAC ACCATACAGG GAAACGCCCA CAGCATTGGG ATTGTTCAGA GGTCGCTGAG CCTCCTGCAT AGGAATGCAG ATCTCAGGAT TTCCCTTTCC TTTGTTGAGA TATACAACGA GACGATGCTG GACCTGTTTG ACCCCGAGGC TATCCTATCG ATACGGGAAG ACCCGCTTTG TGGGGTGGTT GTTGACGGCC TGACTATTGT GGACTGCGAC AGCTACGAGA AGAGCATGGA GATGTATTCC AGAGGGACAA GGACAAGGAG GACGTCCTCC ACGTCGATGA ACAAGGAGAG CAGCAGAAGC CACTCTGTTT TCACTGTGTA TCTCAAGAGC AGCGGGGAAG TGATGTCGAG GTCGTCGAGG GTGTGCTTTG TTGATCTTGC AGGGTCTGAG AGGCTGAGGG AGAGAGAGGT GGAGGAGACG AAAATGAAGG AGACTGCCAA CATAAACAAG TCTCTCCTGT ACCTTGGAAA GGTGATCAAC AAGCTCAGCA ACGGTGAGGA GGGGCACATA GGATATAGAG AGTCCAAGCT TACTTTCCTA CTCAAGGACT CGCTGGGGGG AAACTGCAAG CTGACGGTCA TCGGAAATGT CTCTCTGGAG AGTAGAAGCG ACAGCATAAA CACAATGAAC TTTCTTCAGA GATCCAAGAT GATAAGGAAC CTTGCGGTTG TGAACTCCGA TGTAAATGGA GAGTTGGAAG AGGTGAAGAG CAGGCTGAAG ACCCTGGACA GCGAGAACCA GAGCCTGAAG GCCCGCATTG CTCTTATAGA CAGCCAGATC CAGGAGGAGT CTAGGGCTCT TCCTTCGTAC CATTATGACA TCAAAAGGGT GAAGGATGCA GTCGACGACA TCATGAGCAC TGTTCTGGAT CTAGGAGAGA CCGTTGGGAG GGTATCAGGG GTCGAGTTTG ACAAGAACAG AAGGCTTCTT CTCAGCATCC ACGAGTGCTT CATGTCGATG CACAGAAGGC AAGAGGATGC AGAAAGCTCC ACGAAGAGAA AGAGGAGGGA CGCGAAGGAG
Details for EncuA.00523.a.A1.GE27212
HARVESTED ON: data unavailable
SEQUENCED ON: 8/12/2009
EXPECTED MW: 49kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 93
Validated AA Sequence
MLENVRTYLR IKPQAIPEDI VVGGDSVIVD GSSFCFDRVF RGSTQQEVFQ EISKSLLVEC MQGYNCTLFA YGQTGSGKTY TIQGNAHSIG IVQRSLSLLH RNADLRISLS FVEIYNETML DLFDPEAILS IREDPLCGVV VDGLTIVDCD SYEXSMEMYS RGTRTRRTSS TSMNKESSRS HSVFTVYLKS SGEVMSRSSR VCFVDLAGSE RLREREVEET KMKETANINK SLLYLGKVIN KLSNGEEGHI GYRESKLTFL LKDSLGGNCK LTVIGNVSLE SRSDSINTMN FLQRSKMIRN LAVVNSDVNG ELEEVKSRLK TLDSENQSLK ARIALIDSQI QEESRALPSY HYDIKRVKDA VDDIMSTVLD LGETVGRVSG VEFDKNRRLL LSIHECFMSM HRRQEDAESS TKRK
Validated NT Sequence
ccctggaagc tcnnacccag ggtcctggtt cgatgcttga aaatgtcagg acatatctga ggatcaagcc acaggcaatt ccagaggaca tagttgttgg cggagactca gtaatcgtgg atggaagcag cttctgcttc gacagggttt tcagaggctc cacgcagcaa gaggttttcc aggagatatc aaagagtctt ctggtggagt gcatgcaggg atacaactgc acgctgtttg catatggaca aacagggagc gggaagacct acaccataca gggaaacgcc cacagcattg ggattgttca gaggtcgctg agcctcctgc ataggaatgc agatctcagg atttcccttt cctttgttga gatatacaac gagacgatgc tggacctgtt tgaccccgag gctatcctat cgatacggga agacccgctt tgtggggtgg ttgttgacgg cctgactatt gtggactgcg acagctacga gangagcatg gagatgtatt ccagagggac aaggacaagg aggacgtcct ccacgtcgat gaacaaggag agcagcagaa gccactctgt tttcactgtg tatctcaaga gcagcgggga agtgatgtcg aggtcgtcga gggtgtgctt tgttgatctt gcagggtctg agaggctgag ggagagagag gtggaggaga cgaaaatgaa ggagactgcc aacataaaca agtctctcct gtaccttgga aaggtgatca acaagctcag caacggtgag gaggggcaca taggatatag agagtccaag cttactttcc tactcaagga ctcgctgggg ggaaactgca agctgacggt catcggaaat gtctctctgg agagtagaag cgacagcata aacacaatga actttcttca gagatccaag atgataagga accttgcggt tgtgaactcc gatgtaaatg gagagttgga agaggtgaag agcaggctga agaccctgga cagcgagaac cagagcctga aggcccgcat tgctcttata gacagccaga tccaggagga gtctagggct cttccttcgt accattatga catcaaaagg gtgaaggatg cagtngacga catcatgagc actgttctgg atctaggaga gaccgttggg agggtatcag gggtcgagtt tgacaagaac agaaggcttc ttctcagcat ccacgagtgc ttcatgtcga tgcacagaag gcaagaggat gcagaaagct ccacgaagag aaagagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLENVRTYL RIKPQAIPED IVVGGDSVIV DGSSFCFDRV FRGSTQQEVF QEISKSLLVE CMQGYNCTLF AYGQTGSGKT YTIQGNAHSI GIVQRSLSLL HRNADLRISL SFVEIYNETM LDLFDPEAIL SIREDPLCGV VVDGLTIVDC DSYEKSMEMY SRGTRTRRTS STSMNKESSR SHSVFTVYLK SSGEVMSRSS RVCFVDLAGS ERLREREVEE TKMKETANIN KSLLYLGKVI NKLSNGEEGH IGYRESKLTF LLKDSLGGNC KLTVIGNVSL ESRSDSINTM NFLQRSKMIR NLAVVNSDVN GELEEVKSRL KTLDSENQSL KARIALIDSQ IQEESRALPS YHYDIKRVKD AVDDIMSTVL DLGETVGRVS GVEFDKNRRL LLSIHECFMS MHRRQEDAES STKRKRRDAK E
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcttgaaaat gtcaggacat atctgaggat caagccacag gcaattccag aggacatagt tgttggcgga gactcagtaa tcgtggatgg aagcagcttc tgcttcgaca gggttttcag aggctccacg cagcaagagg ttttccagga gatatcaaag agtcttctgg tggagtgcat gcagggatac aactgcacgc tgtttgcata tggacaaaca gggagcggga agacctacac catacaggga aacgcccaca gcattgggat tgttcagagg tcgctgagcc tcctgcatag gaatgcagat ctcaggattt ccctttcctt tgttgagata tacaacgaga cgatgctgga cctgtttgac cccgaggcta tcctatcgat acgggaagac ccgctttgtg gggtggttgt tgacggcctg actattgtgg actgcgacag ctacgagaag agcatggaga tgtattccag agggacaagg acaaggagga cgtcctccac gtcgatgaac aaggagagca gcagaagcca ctctgttttc actgtgtatc tcaagagcag cggggaagtg atgtcgaggt cgtcgagggt gtgctttgtt gatcttgcag ggtctgagag gctgagggag agagaggtgg aggagacgaa aatgaaggag actgccaaca taaacaagtc tctcctgtac cttggaaagg tgatcaacaa gctcagcaac ggtgaggagg ggcacatagg atatagagag tccaagctta ctttcctact caaggactcg ctggggggaa actgcaagct gacggtcatc ggaaatgtct ctctggagag tagaagcgac agcataaaca caatgaactt tcttcagaga tccaagatga taaggaacct tgcggttgtg aactccgatg taaatggaga gttggaagag gtgaagagca ggctgaagac cctggacagc gagaaccaga gcctgaaggc ccgcattgct cttatagaca gccagatcca ggaggagtct agggctcttc cttcgtacca ttatgacatc aaaagggtga aggatgcagt cgacgacatc atgagcactg ttctggatct aggagagacc gttgggaggg tatcaggggt cgagtttgac aagaacagaa ggcttcttct cagcatccac gagtgcttca tgtcgatgca cagaaggcaa gaggatgcag aaagctccac gaagagaaag aggagggacg cgaaggagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa