EncuA.00500.a

SOMA FERRITIN

CENTER ID: EncuA.00500.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00500.a.A1.GE28315 Full length( EncuA.00500.a ) 1 176
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU06_1380
RefSeq: NP_585894.1
UniProt: Q8SRN5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDPKTKQASS DEWNVEAGLL LSSQLLLEYN AYYFYSACAA HFSRSDVCLK GLASFFRKKS LDENAQAQKI IGFMNMRELD IEFRAIEAPD IKKYGKTSGV LKACKEFEQT VLSNISTISE IARKAGDGAI VQFLEDFIAE QVRSISELSD LCANCARCGD GGVGLFLFDQ SLISQH
NT Sequence
ATGGATCCAA AGACAAAGCA GGCTTCATCT GACGAGTGGA ATGTAGAGGC CGGATTGCTT CTCTCCTCTC AGCTGCTGCT CGAATACAAT GCATACTATT TCTATTCAGC ATGTGCAGCC CACTTCAGCA GGTCTGACGT CTGCCTTAAG GGACTGGCCA GCTTCTTCAG GAAGAAAAGC CTTGATGAGA ATGCCCAGGC CCAAAAGATT ATTGGCTTCA TGAATATGAG AGAGTTAGAT ATTGAGTTCA GAGCCATCGA GGCGCCAGAT ATCAAGAAGT ACGGAAAGAC GAGCGGCGTT CTCAAGGCTT GCAAGGAATT CGAGCAAACT GTGCTCAGTA ACATATCGAC CATTTCTGAA ATAGCCAGAA AAGCTGGAGA CGGGGCCATC GTCCAGTTCC TCGAGGACTT CATCGCCGAG CAGGTGAGGT CTATTTCGGA GCTTAGTGAT CTATGCGCCA ACTGTGCAAG GTGTGGGGAC GGTGGCGTGG GGCTATTCCT CTTTGACCAA TCACTGATAA GCCAACAT
Details for EncuA.00500.a.A1.GE28315
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 21kDa
OBSERVED MW: 19kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDPKTKQAS SDEWNVEAGL LLSSQLLLEY NAYYFYSACA AHFSRSDVCL KGLASFFRKK SLDENAQAQK IIGFMNMREL DIEFRAIEAP DIKKYGKTSG VLKACKEFEQ TVLSNISTIS EIARKAGDGA IVQFLEDFIA EQVRSISELS DLCANCARCG DGGVGLFLFD QSLI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggatc caaagacaaa gcaggcttca tctgacgagt ggaatgtaga ggccggattg cttctctcct ctcagctgct gctcgaatac aatgcatact atttctattc agcatgtgca gcccacttca gcaggtctga cgtctgcctt aagggactgg ccagcttctt caggaagaaa agccttgatg agaatgccca ggcccaaaag attattggct tcatgaatat gagagagtta gatattgagt tcagagccat cgaggcgcca gatatcaaga agtacggaaa gacgagcggc gttctcaagg cttgcaagga attcgagcaa actgtgctca gtaacatatc gaccatttct gaaatagcca gaaaagctgg agacggggcc atcgtccagt tcctcgagga cttcatcgcc gagcaggtga ggtctatttc ggagcttagt gatctatgcg ccaactgtgc aaggtgtggg gacggtggcg tggggctatt cctctttgac caatcactga taag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDPKTKQAS SDEWNVEAGL LLSSQLLLEY NAYYFYSACA AHFSRSDVCL KGLASFFRKK SLDENAQAQK IIGFMNMREL DIEFRAIEAP DIKKYGKTSG VLKACKEFEQ TVLSNISTIS EIARKAGDGA IVQFLEDFIA EQVRSISELS DLCANCARCG DGGVGLFLFD QSLISQH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatccaaag acaaagcagg cttcatctga cgagtggaat gtagaggccg gattgcttct ctcctctcag ctgctgctcg aatacaatgc atactatttc tattcagcat gtgcagccca cttcagcagg tctgacgtct gccttaaggg actggccagc ttcttcagga agaaaagcct tgatgagaat gcccaggccc aaaagattat tggcttcatg aatatgagag agttagatat tgagttcaga gccatcgagg cgccagatat caagaagtac ggaaagacga gcggcgttct caaggcttgc aaggaattcg agcaaactgt gctcagtaac atatcgacca tttctgaaat agccagaaaa gctggagacg gggccatcgt ccagttcctc gaggacttca tcgccgagca ggtgaggtct atttcggagc ttagtgatct atgcgccaac tgtgcaaggt gtggggacgg tggcgtgggg ctattcctct ttgaccaatc actgataagc caacataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa