EncuA.00490.a

Probable hydroxyacylglutathione hydrolase ECU02_0580

CENTER ID: EncuA.00490.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00490.a.A1.GE30861 Full length( EncuA.00490.a ) 1 263
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00490.a.A1.PS00981 Full length( EncuA.00490.a ) 1 263

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU02_0580
RefSeq: NP_584585.1
UniProt: Q8SSH0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MILYYKKTTK VPLNMGIEVI SILVGGGNNY MHLFYDDDAA FCVDASNPRI LLKALGINFK KSIYDEKEIF SMGEDRKKRR ELVYLFTTHK HLDHSAGIRC ISEESPNTKT ISGFSGDICK SGDKFRFKDV EIECFHTPCH TVDSFCFYVG EKYLATGDTL LFLGCGKFFG GTPGQMIKNI EEIKKRVNHD AVLLYGHDYS EQNIRFTEEF YHVPEEIKKK KFLKLAEEIK YNPFFNLKKV SIEGSEEEVM GKLRERKNKF SKE
NT Sequence
ATGATCCTGT ACTATAAAAA AACAACAAAA GTACCCTTAA ACATGGGCAT TGAAGTTATA AGCATACTTG TAGGGGGAGG AAACAACTAC ATGCATCTAT TCTACGACGA TGATGCTGCA TTCTGTGTGG ATGCAAGCAA CCCAAGGATA CTTCTGAAGG CCCTAGGGAT CAACTTCAAG AAGAGTATCT ATGACGAAAA GGAGATTTTC TCTATGGGGG AGGACCGGAA GAAGAGAAGA GAGCTTGTTT ACCTGTTCAC CACGCACAAG CACCTGGACC ACTCTGCGGG GATTCGATGT ATATCAGAGG AAAGCCCAAA CACCAAGACG ATATCCGGGT TTTCGGGAGA CATATGCAAG TCCGGGGACA AGTTCCGCTT CAAAGATGTG GAGATAGAGT GTTTCCACAC GCCCTGCCAT ACAGTGGACA GCTTCTGCTT CTATGTTGGC GAGAAATACC TTGCTACGGG AGACACCTTA CTCTTCCTCG GATGCGGAAA GTTCTTCGGA GGAACTCCTG GACAAATGAT CAAAAACATT GAAGAGATAA AGAAAAGGGT AAACCATGAT GCTGTGCTAC TATATGGGCA TGACTATAGC GAGCAGAACA TCCGCTTTAC TGAGGAGTTT TACCATGTGC CCGAGGAGAT AAAGAAGAAG AAGTTCCTGA AGTTGGCGGA GGAAATCAAG TACAACCCAT TCTTTAACCT GAAGAAGGTT TCCATAGAGG GCTCGGAAGA AGAGGTAATG GGGAAACTCC GGGAGAGGAA GAACAAGTTT AGTAAGGAA
Details for EncuA.00490.a.A1.GE30861
HARVESTED ON: 11/16/2010
SEQUENCED ON: 11/23/2010
EXPECTED MW: 32kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 91
Validated AA Sequence
MILYYKKTTK VPLNMGIEVI SILVGGGNNY MHLFYDDDAA FCVDASNPRI LLKALGINFK KSIYDEKEIF SMGEDRKKRR ELVYLFTTHK HLDHSAGIRC ISEESPNTKT ISGFSGDICK SGDKFRFKDV EIECFHTPCH TVDSFCFYVG EKYLATGDTL LFLGCGKFFG GTPGQMIKNI EEIKKRVNHD AVLLYGHDYS EQNIRFTEEF YHVPEEIKKK KFLKLAEEIK YNPFFNLKKV SIEGSEEEVM GKLRERKNKF S
Validated NT Sequence
tactaaactt gttcttcctc tcccggagtt tccccattac ctcttcttcc gagccctcta tggaaacctt cttcaggtta aagaatgggt tgtacttgat ttcctccgcc aacttcagga acttcttctt ctttatctcc tcgggcacat ggtaaaactc ctcagtaaag cggatgttct gctcgctata gtcatgccca tatagtagca cagcatcatg gtttaccctt ttctttatct cttcaatgtt tttgatcatt tgtccaggag ttcctccgaa gaactttccg catccgagga agagtaaggt gtctcccgta gcaaggtatt tctcgccaac atagaagcag aagctgtcca ctgtatggca gggcgtgtgg aaacactcta tctccacatc tttgaagcgg aacttgtccc cggacttgca tatgtctccc gaaaacccgg atatcgtctt ggtgtttggg ctttcctctg atatacatcg aatccccgca gagtggtcca ggtgcttgtg cgtggtgaac aggtaaacaa gctctcttct cttcttccgg tcctccccca tagagaaaat ctccttttcg tcatagatac tcttcttgaa gttgatccct agggccttca gaagtatcct tgggttgctt gcatccacac agaatgcagc atcatcgtcg tagaatagat gcatgtagtt gtttcctccc cctacaagta tgcttataac ttcaatgccc atgtttaagg gtacttttgt tgttttttta tagtacagga tcatcgaacc aggaccctgg gtctgagctt ccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMILYYKKTT KVPLNMGIEV ISILVGGGNN YMHLFYDDDA AFCVDASNPR ILLKALGINF KKSIYDEKEI FSMGEDRKKR RELVYLFTTH KHLDHSAGIR CISEESPNTK TISGFSGDIC KSGDKFRFKD VEIECFHTPC HTVDSFCFYV GEKYLATGDT LLFLGCGKFF GGTPGQMIKN IEEIKKRVNH DAVLLYGHDY SEQNIRFTEE FYHVPEEIKK KKFLKLAEEI KYNPFFNLKK VSIEGSEEEV MGKLRERKNK FSKE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcctgtac tataaaaaaa caacaaaagt acccttaaac atgggcattg aagttataag catacttgta gggggaggaa acaactacat gcatctattc tacgacgatg atgctgcatt ctgtgtggat gcaagcaacc caaggatact tctgaaggcc ctagggatca acttcaagaa gagtatctat gacgaaaagg agattttctc tatgggggag gaccggaaga agagaagaga gcttgtttac ctgttcacca cgcacaagca cctggaccac tctgcgggga ttcgatgtat atcagaggaa agcccaaaca ccaagacgat atccgggttt tcgggagaca tatgcaagtc cggggacaag ttccgcttca aagatgtgga gatagagtgt ttccacacgc cctgccatac agtggacagc ttctgcttct atgttggcga gaaatacctt gctacgggag acaccttact cttcctcgga tgcggaaagt tcttcggagg aactcctgga caaatgatca aaaacattga agagataaag aaaagggtaa accatgatgc tgtgctacta tatgggcatg actatagcga gcagaacatc cgctttactg aggagtttta ccatgtgccc gaggagataa agaagaagaa gttcctgaag ttggcggagg aaatcaagta caacccattc tttaacctga agaaggtttc catagagggc tcggaagaag aggtaatggg gaaactccgg gagaggaaga acaagtttag taaggaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for EncuA.00490.a.A1.PS00981
PURIFICATION DATe: 2/22/2011
CONCENTRATION: 21.66mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 91
Protocol Notes
notes unavailable
Validated AA Sequence
MILYYKKTTK VPLNMGIEVI SILVGGGNNY MHLFYDDDAA FCVDASNPRI LLKALGINFK KSIYDEKEIF SMGEDRKKRR ELVYLFTTHK HLDHSAGIRC ISEESPNTKT ISGFSGDICK SGDKFRFKDV EIECFHTPCH TVDSFCFYVG EKYLATGDTL LFLGCGKFFG GTPGQMIKNI EEIKKRVNHD AVLLYGHDYS EQNIRFTEEF YHVPEEIKKK KFLKLAEEIK YNPFFNLKKV SIEGSEEEVM GKLRERKNKF S
Validated NT Sequence
tactaaactt gttcttcctc tcccggagtt tccccattac ctcttcttcc gagccctcta tggaaacctt cttcaggtta aagaatgggt tgtacttgat ttcctccgcc aacttcagga acttcttctt ctttatctcc tcgggcacat ggtaaaactc ctcagtaaag cggatgttct gctcgctata gtcatgccca tatagtagca cagcatcatg gtttaccctt ttctttatct cttcaatgtt tttgatcatt tgtccaggag ttcctccgaa gaactttccg catccgagga agagtaaggt gtctcccgta gcaaggtatt tctcgccaac atagaagcag aagctgtcca ctgtatggca gggcgtgtgg aaacactcta tctccacatc tttgaagcgg aacttgtccc cggacttgca tatgtctccc gaaaacccgg atatcgtctt ggtgtttggg ctttcctctg atatacatcg aatccccgca gagtggtcca ggtgcttgtg cgtggtgaac aggtaaacaa gctctcttct cttcttccgg tcctccccca tagagaaaat ctccttttcg tcatagatac tcttcttgaa gttgatccct agggccttca gaagtatcct tgggttgctt gcatccacac agaatgcagc atcatcgtcg tagaatagat gcatgtagtt gtttcctccc cctacaagta tgcttataac ttcaatgccc atgtttaagg gtacttttgt tgttttttta tagtacagga tcatcgaacc aggaccctgg gtctgagctt ccaggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMILYYKKTT KVPLNMGIEV ISILVGGGNN YMHLFYDDDA AFCVDASNPR ILLKALGINF KKSIYDEKEI FSMGEDRKKR RELVYLFTTH KHLDHSAGIR CISEESPNTK TISGFSGDIC KSGDKFRFKD VEIECFHTPC HTVDSFCFYV GEKYLATGDT LLFLGCGKFF GGTPGQMIKN IEEIKKRVNH DAVLLYGHDY SEQNIRFTEE FYHVPEEIKK KKFLKLAEEI KYNPFFNLKK VSIEGSEEEV MGKLRERKNK FSKE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATCCTGTAC TATAAAAAAA CAACAAAAGT ACCCTTAAAC ATGGGCATTG AAGTTATAAG CATACTTGTA GGGGGAGGAA ACAACTACAT GCATCTATTC TACGACGATG ATGCTGCATT CTGTGTGGAT GCAAGCAACC CAAGGATACT TCTGAAGGCC CTAGGGATCA ACTTCAAGAA GAGTATCTAT GACGAAAAGG AGATTTTCTC TATGGGGGAG GACCGGAAGA AGAGAAGAGA GCTTGTTTAC CTGTTCACCA CGCACAAGCA CCTGGACCAC TCTGCGGGGA TTCGATGTAT ATCAGAGGAA AGCCCAAACA CCAAGACGAT ATCCGGGTTT TCGGGAGACA TATGCAAGTC CGGGGACAAG TTCCGCTTCA AAGATGTGGA GATAGAGTGT TTCCACACGC CCTGCCATAC AGTGGACAGC TTCTGCTTCT ATGTTGGCGA GAAATACCTT GCTACGGGAG ACACCTTACT CTTCCTCGGA TGCGGAAAGT TCTTCGGAGG AACTCCTGGA CAAATGATCA AAAACATTGA AGAGATAAAG AAAAGGGTAA ACCATGATGC TGTGCTACTA TATGGGCATG ACTATAGCGA GCAGAACATC CGCTTTACTG AGGAGTTTTA CCATGTGCCC GAGGAGATAA AGAAGAAGAA GTTCCTGAAG TTGGCGGAGG AAATCAAGTA CAACCCATTC TTTAACCTGA AGAAGGTTTC CATAGAGGGC TCGGAAGAAG AGGTAATGGG GAAACTCCGG GAGAGGAAGA ACAAGTTTAG TAAGGAATAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA