EncuA.00440.a
Fructose-bisphosphate aldolase
CENTER ID:
EncuA.00440.a
ORGANISM:
Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE:
Microsporidiosis
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
False
NIH RISK GROUP:
2
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
I/IIIB
Ordering Clones & Proteins
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
EncuA.00440.a.A1.GE27206
Full length( EncuA.00440.a )
info
1
338
EncuA.00440.a.A1.GE27206
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
EncuA.00440.a.A1.PW27195
Full length( EncuA.00440.a )
info
1
338
PW27195
EncuA.00440.a.A1.PW27477
Full length( EncuA.00440.a )
info
1
338
PW27477
Structures
3MBD
DEPOSITED: 3/25/2010
DETERMINATION: XRay
CLONE: EncuA.00440.a.A1.GE27206
PROTEIN: EncuA.00440.a.A1.PW27477
3MBF
DEPOSITED: 3/25/2010
DETERMINATION: XRay
CLONE: EncuA.00440.a.A1.GE27206
PROTEIN: EncuA.00440.a.A1.PW27477
3QRH
DEPOSITED: 2/17/2011
DETERMINATION: XRay
CLONE: EncuA.00440.a.A1.GE27206
PROTEIN: EncuA.00440.a.A1.PW27477
Publications by SSGCID
Structure of fructose bisphosphate aldolase from Encephalitozoon cuniculi.
Bhandari J, Davies D, Gardberg A, Sankaran B, Staker B, Stewart L
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1055-9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMDCDHLLRL GMTAKKILEN GKGILAADET PKTLGRRFEK LGITNTEENR RKFREILFST KGIERYIGGV ILNQETFEQT SGSGVPLTEL LKKKGIEIGI KLDKGLIDYK EKEKISVGLE DLDLRCKSSA FKDATFAKWR SLFYFYDGIP SEDCINENCS ILAKYAIICQ KNGLVPIVEP EVFLEGDYSM KRSYEVTRQI LSTLMKYLNY ELVYIPGVLI KASYVTSGQL SNEKYTPKKV ATFTLRALLS TIPCGIPGIV FLSGGHGSED AIGFLNAINM ERGCRTWSLS FSFARALTDG VLETWRGDDS NIEEAQKILL ETSFKACRGA EGKLWDQE
NT Sequence
ATGATGGACT GTGATCATCT TTTAAGGCTT GGAATGACTG CAAAAAAGAT ATTGGAAAAC GGGAAAGGGA TTCTCGCGGC CGATGAAACC CCAAAGACGT TGGGCAGAAG ATTTGAAAAG CTTGGGATCA CGAATACCGA AGAGAATAGA AGGAAGTTCA GAGAAATACT ATTTAGCACT AAGGGAATTG AAAGGTACAT TGGCGGGGTT ATCCTAAATC AAGAAACATT CGAGCAGACA AGTGGAAGTG GAGTGCCACT AACAGAGCTG CTAAAGAAAA AGGGCATCGA AATAGGCATA AAGCTCGATA AAGGGCTGAT AGACTACAAG GAAAAGGAAA AGATCTCTGT AGGGCTTGAG GACTTGGACC TAAGGTGCAA GTCGTCGGCA TTCAAAGACG CCACATTTGC CAAGTGGAGG TCTCTGTTCT ACTTCTACGA CGGAATTCCC TCGGAAGACT GCATCAACGA AAACTGTTCC ATCCTGGCAA AATACGCAAT AATATGCCAG AAGAATGGGC TTGTCCCGAT TGTGGAGCCT GAGGTATTTC TTGAAGGGGA CTACAGCATG AAGAGATCAT ACGAGGTAAC CAGGCAGATA TTAAGCACTC TAATGAAGTA CTTAAACTAC GAATTGGTTT ACATCCCAGG AGTACTCATT AAGGCAAGCT ATGTCACGTC CGGACAACTT AGCAACGAAA AATATACGCC AAAGAAGGTT GCCACCTTCA CACTCAGGGC TCTTCTCTCA ACCATTCCCT GTGGAATTCC AGGCATCGTT TTTCTGAGTG GTGGACATGG CTCGGAAGAT GCAATAGGCT TTCTCAATGC AATAAACATG GAAAGAGGAT GCAGAACATG GAGCTTGAGC TTTAGCTTTG CGAGGGCGTT GACAGATGGA GTTCTAGAAA CATGGAGGGG AGATGACTCA AATATTGAAG AAGCACAGAA AATATTGCTA GAGACATCGT TCAAAGCTTG TAGGGGAGCA GAGGGAAAGC TGTGGGATCA GGAG
Details for EncuA.00440.a.A1.GE27206
HARVESTED ON:
7/31/2009
SEQUENCED ON:
8/12/2009
EXPECTED MW:
40kDa
OBSERVED MW:
40kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
pass
PERCENT IDENTITY:
99
PERCENT COVERAGE:
96
Details for EncuA.00440.a.A1.PW27195
PURIFICATION DATe:
8/18/2009
CONCENTRATION:
23.06mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
4
VIAL VOLUME:
50µl
PERCENT IDENTITY:
99
PERCENT COVERAGE:
96
Details for EncuA.00440.a.A1.PW27477
PURIFICATION DATe:
8/18/2009
CONCENTRATION:
24.72mg/ml
OBSERVED MW:
data unavailable
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
21
VIAL VOLUME:
200µl
PERCENT IDENTITY:
99
PERCENT COVERAGE:
96