EncuA.00440.a

Fructose-bisphosphate aldolase

CENTER ID: EncuA.00440.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
EncuA.00440.a.A1.GE27206 Full length( EncuA.00440.a ) 1 338
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
EncuA.00440.a.A1.PW27195 Full length( EncuA.00440.a ) 1 338
EncuA.00440.a.A1.PW27477 Full length( EncuA.00440.a ) 1 338

Structures

3MBD
DEPOSITED: 3/25/2010
DETERMINATION: XRay
CLONE: EncuA.00440.a.A1.GE27206
PROTEIN: EncuA.00440.a.A1.PW27477
3MBF
DEPOSITED: 3/25/2010
DETERMINATION: XRay
CLONE: EncuA.00440.a.A1.GE27206
PROTEIN: EncuA.00440.a.A1.PW27477
3QRH
DEPOSITED: 2/17/2011
DETERMINATION: XRay
CLONE: EncuA.00440.a.A1.GE27206
PROTEIN: EncuA.00440.a.A1.PW27477

Publications by SSGCID

Structure of fructose bisphosphate aldolase from Encephalitozoon cuniculi.
Bhandari J, Davies D, Gardberg A, Sankaran B, Staker B, Stewart L
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. - 2011
volume 67, issue Pt 9, pages 1055-9
PMID: 21904050; PMCID: PMC3169402
PDB: 3MBD, 3MBF

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU01_0240
OrthoMCL: OG5_127143
RefSeq: XP_965859.1
UniProt: Q8SSM8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMDCDHLLRL GMTAKKILEN GKGILAADET PKTLGRRFEK LGITNTEENR RKFREILFST KGIERYIGGV ILNQETFEQT SGSGVPLTEL LKKKGIEIGI KLDKGLIDYK EKEKISVGLE DLDLRCKSSA FKDATFAKWR SLFYFYDGIP SEDCINENCS ILAKYAIICQ KNGLVPIVEP EVFLEGDYSM KRSYEVTRQI LSTLMKYLNY ELVYIPGVLI KASYVTSGQL SNEKYTPKKV ATFTLRALLS TIPCGIPGIV FLSGGHGSED AIGFLNAINM ERGCRTWSLS FSFARALTDG VLETWRGDDS NIEEAQKILL ETSFKACRGA EGKLWDQE
NT Sequence
ATGATGGACT GTGATCATCT TTTAAGGCTT GGAATGACTG CAAAAAAGAT ATTGGAAAAC GGGAAAGGGA TTCTCGCGGC CGATGAAACC CCAAAGACGT TGGGCAGAAG ATTTGAAAAG CTTGGGATCA CGAATACCGA AGAGAATAGA AGGAAGTTCA GAGAAATACT ATTTAGCACT AAGGGAATTG AAAGGTACAT TGGCGGGGTT ATCCTAAATC AAGAAACATT CGAGCAGACA AGTGGAAGTG GAGTGCCACT AACAGAGCTG CTAAAGAAAA AGGGCATCGA AATAGGCATA AAGCTCGATA AAGGGCTGAT AGACTACAAG GAAAAGGAAA AGATCTCTGT AGGGCTTGAG GACTTGGACC TAAGGTGCAA GTCGTCGGCA TTCAAAGACG CCACATTTGC CAAGTGGAGG TCTCTGTTCT ACTTCTACGA CGGAATTCCC TCGGAAGACT GCATCAACGA AAACTGTTCC ATCCTGGCAA AATACGCAAT AATATGCCAG AAGAATGGGC TTGTCCCGAT TGTGGAGCCT GAGGTATTTC TTGAAGGGGA CTACAGCATG AAGAGATCAT ACGAGGTAAC CAGGCAGATA TTAAGCACTC TAATGAAGTA CTTAAACTAC GAATTGGTTT ACATCCCAGG AGTACTCATT AAGGCAAGCT ATGTCACGTC CGGACAACTT AGCAACGAAA AATATACGCC AAAGAAGGTT GCCACCTTCA CACTCAGGGC TCTTCTCTCA ACCATTCCCT GTGGAATTCC AGGCATCGTT TTTCTGAGTG GTGGACATGG CTCGGAAGAT GCAATAGGCT TTCTCAATGC AATAAACATG GAAAGAGGAT GCAGAACATG GAGCTTGAGC TTTAGCTTTG CGAGGGCGTT GACAGATGGA GTTCTAGAAA CATGGAGGGG AGATGACTCA AATATTGAAG AAGCACAGAA AATATTGCTA GAGACATCGT TCAAAGCTTG TAGGGGAGCA GAGGGAAAGC TGTGGGATCA GGAG
Details for EncuA.00440.a.A1.GE27206
HARVESTED ON: 7/31/2009
SEQUENCED ON: 8/12/2009
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMMDCDHL LRLGMTAKKI LENGKGILAA DETPKTLGRX FEKLGITNTE ENRRKFREIL FSTKGIERYI GGVILNQETF EQTSGSGVPL TELLKKKGIE IGIKLDKGLI DYKEKEKISV GLEDLDLRCK SSAFKDATFA KWRSLFYFYD GIPSEDCINE NCSILAKYAI ICQKNGLVPI VEPEVFLEGD YSMKRSYEVT RQILSTLMKY LNYELVYIPG VLIKASYVTS GQLSNEKYTP KKVATFTLRA LLSTIPCGIP GIVFLSGGHG SEDAIGFLNA INMERGCRTW SLSFSFARAL TDGVLETWRG DDSNIEEAQK ILLETSFKAC RGAEGKLW
Validated NT Sequence
atcccacagc tttccctctg ctcccctaca agctttgaac gatgtctcta gcaatatttt ctgtgcttct tcaatatttg agtcatctcc cctccatgtt tctagaactc catctgtcaa cgccctcgca aagctaaagc tcaagctcca tgttctgcat cctctttcca tgtttattgc attgagaaag cctattgcat cttccgagcc atgtccacca ctcagaaaaa cgatgcctgg aattccacag ggaatggttg agagaagagc cctgagtgtg aaggtggcaa ccttctttgg cgtatatttt tcgttgctaa gttgtccgga cgtgacatag cttgccttaa tgagtactcc tgggatgtaa accaattcgt agtttaagta cttcattaga gtgcttaata tctgcctggt tacctcgtat gatctcttca tgctgtagtc cccttcaaga aatacctcag gctccacaat cgggacaagc ccattcttct ggcatattat tgcgtatttt gccaggatgg aacagttttc gttgatgcag tcttccgagg gaattccgtc gtagaagtag aacagagacc tccacttggc aaatgtggcg tctttgaatg ccgacgactt gcaccttagg tccaagtcct caagccctac agagatcttt tccttttcct tgtagtctat cagcccttta tcgagcttta tgcctatttc gatgcccttt ttctttagca gctctgttag tggcactcca cttccacttg tctgctcgaa tgtttcttga tttaggataa ccccgccaat gtacctttca attcccttag tgctaaatag tatttctctg aacttccttc tattctcttc ggtattcgtg atcccaagct tttcaaatnt tctgcccaac gtctttgggg tttcatcggc cgcgagaatc cctttcccgt tttccaatat cttttttgca gtcattccaa gccttaaaag atgatcacag tccatcatcg aaccaggacc ctgggtctga gcttccaggg tacccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMDCDHLLR LGMTAKKILE NGKGILAADE TPKTLGRRFE KLGITNTEEN RRKFREILFS TKGIERYIGG VILNQETFEQ TSGSGVPLTE LLKKKGIEIG IKLDKGLIDY KEKEKISVGL EDLDLRCKSS AFKDATFAKW RSLFYFYDGI PSEDCINENC SILAKYAIIC QKNGLVPIVE PEVFLEGDYS MKRSYEVTRQ ILSTLMKYLN YELVYIPGVL IKASYVTSGQ LSNEKYTPKK VATFTLRALL STIPCGIPGI VFLSGGHGSE DAIGFLNAIN MERGCRTWSL SFSFARALTD GVLETWRGDD SNIEEAQKIL LETSFKACRG AEGKLWDQE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatggactgt gatcatcttt taaggcttgg aatgactgca aaaaagatat tggaaaacgg gaaagggatt ctcgcggccg atgaaacccc aaagacgttg ggcagaagat ttgaaaagct tgggatcacg aataccgaag agaatagaag gaagttcaga gaaatactat ttagcactaa gggaattgaa aggtacattg gcggggttat cctaaatcaa gaaacattcg agcagacaag tggaagtgga gtgccactaa cagagctgct aaagaaaaag ggcatcgaaa taggcataaa gctcgataaa gggctgatag actacaagga aaaggaaaag atctctgtag ggcttgagga cttggaccta aggtgcaagt cgtcggcatt caaagacgcc acatttgcca agtggaggtc tctgttctac ttctacgacg gaattccctc ggaagactgc atcaacgaaa actgttccat cctggcaaaa tacgcaataa tatgccagaa gaatgggctt gtcccgattg tggagcctga ggtatttctt gaaggggact acagcatgaa gagatcatac gaggtaacca ggcagatatt aagcactcta atgaagtact taaactacga attggtttac atcccaggag tactcattaa ggcaagctat gtcacgtccg gacaacttag caacgaaaaa tatacgccaa agaaggttgc caccttcaca ctcagggctc ttctctcaac cattccctgt ggaattccag gcatcgtttt tctgagtggt ggacatggct cggaagatgc aataggcttt ctcaatgcaa taaacatgga aagaggatgc agaacatgga gcttgagctt tagctttgcg agggcgttga cagatggagt tctagaaaca tggaggggag atgactcaaa tattgaagaa gcacagaaaa tattgctaga gacatcgttc aaagcttgta ggggagcaga gggaaagctg tgggatcagg agaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for EncuA.00440.a.A1.PW27195
PURIFICATION DATe: 8/18/2009
CONCENTRATION: 23.06mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 50µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMMDCDHL LRLGMTAKKI LENGKGILAA DETPKTLGRX FEKLGITNTE ENRRKFREIL FSTKGIERYI GGVILNQETF EQTSGSGVPL TELLKKKGIE IGIKLDKGLI DYKEKEKISV GLEDLDLRCK SSAFKDATFA KWRSLFYFYD GIPSEDCINE NCSILAKYAI ICQKNGLVPI VEPEVFLEGD YSMKRSYEVT RQILSTLMKY LNYELVYIPG VLIKASYVTS GQLSNEKYTP KKVATFTLRA LLSTIPCGIP GIVFLSGGHG SEDAIGFLNA INMERGCRTW SLSFSFARAL TDGVLETWRG DDSNIEEAQK ILLETSFKAC RGAEGKLW
Validated NT Sequence
atcccacagc tttccctctg ctcccctaca agctttgaac gatgtctcta gcaatatttt ctgtgcttct tcaatatttg agtcatctcc cctccatgtt tctagaactc catctgtcaa cgccctcgca aagctaaagc tcaagctcca tgttctgcat cctctttcca tgtttattgc attgagaaag cctattgcat cttccgagcc atgtccacca ctcagaaaaa cgatgcctgg aattccacag ggaatggttg agagaagagc cctgagtgtg aaggtggcaa ccttctttgg cgtatatttt tcgttgctaa gttgtccgga cgtgacatag cttgccttaa tgagtactcc tgggatgtaa accaattcgt agtttaagta cttcattaga gtgcttaata tctgcctggt tacctcgtat gatctcttca tgctgtagtc cccttcaaga aatacctcag gctccacaat cgggacaagc ccattcttct ggcatattat tgcgtatttt gccaggatgg aacagttttc gttgatgcag tcttccgagg gaattccgtc gtagaagtag aacagagacc tccacttggc aaatgtggcg tctttgaatg ccgacgactt gcaccttagg tccaagtcct caagccctac agagatcttt tccttttcct tgtagtctat cagcccttta tcgagcttta tgcctatttc gatgcccttt ttctttagca gctctgttag tggcactcca cttccacttg tctgctcgaa tgtttcttga tttaggataa ccccgccaat gtacctttca attcccttag tgctaaatag tatttctctg aacttccttc tattctcttc ggtattcgtg atcccaagct tttcaaatnt tctgcccaac gtctttgggg tttcatcggc cgcgagaatc cctttcccgt tttccaatat cttttttgca gtcattccaa gccttaaaag atgatcacag tccatcatcg aaccaggacc ctgggtctga gcttccaggg tacccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMDCDHLLR LGMTAKKILE NGKGILAADE TPKTLGRRFE KLGITNTEEN RRKFREILFS TKGIERYIGG VILNQETFEQ TSGSGVPLTE LLKKKGIEIG IKLDKGLIDY KEKEKISVGL EDLDLRCKSS AFKDATFAKW RSLFYFYDGI PSEDCINENC SILAKYAIIC QKNGLVPIVE PEVFLEGDYS MKRSYEVTRQ ILSTLMKYLN YELVYIPGVL IKASYVTSGQ LSNEKYTPKK VATFTLRALL STIPCGIPGI VFLSGGHGSE DAIGFLNAIN MERGCRTWSL SFSFARALTD GVLETWRGDD SNIEEAQKIL LETSFKACRG AEGKLWDQE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATGGACTGT GATCATCTTT TAAGGCTTGG AATGACTGCA AAAAAGATAT TGGAAAACGG GAAAGGGATT CTCGCGGCCG ATGAAACCCC AAAGACGTTG GGCAGAAGAT TTGAAAAGCT TGGGATCACG AATACCGAAG AGAATAGAAG GAAGTTCAGA GAAATACTAT TTAGCACTAA GGGAATTGAA AGGTACATTG GCGGGGTTAT CCTAAATCAA GAAACATTCG AGCAGACAAG TGGAAGTGGA GTGCCACTAA CAGAGCTGCT AAAGAAAAAG GGCATCGAAA TAGGCATAAA GCTCGATAAA GGGCTGATAG ACTACAAGGA AAAGGAAAAG ATCTCTGTAG GGCTTGAGGA CTTGGACCTA AGGTGCAAGT CGTCGGCATT CAAAGACGCC ACATTTGCCA AGTGGAGGTC TCTGTTCTAC TTCTACGACG GAATTCCCTC GGAAGACTGC ATCAACGAAA ACTGTTCCAT CCTGGCAAAA TACGCAATAA TATGCCAGAA GAATGGGCTT GTCCCGATTG TGGAGCCTGA GGTATTTCTT GAAGGGGACT ACAGCATGAA GAGATCATAC GAGGTAACCA GGCAGATATT AAGCACTCTA ATGAAGTACT TAAACTACGA ATTGGTTTAC ATCCCAGGAG TACTCATTAA GGCAAGCTAT GTCACGTCCG GACAACTTAG CAACGAAAAA TATACGCCAA AGAAGGTTGC CACCTTCACA CTCAGGGCTC TTCTCTCAAC CATTCCCTGT GGAATTCCAG GCATCGTTTT TCTGAGTGGT GGACATGGCT CGGAAGATGC AATAGGCTTT CTCAATGCAA TAAACATGGA AAGAGGATGC AGAACATGGA GCTTGAGCTT TAGCTTTGCG AGGGCGTTGA CAGATGGAGT TCTAGAAACA TGGAGGGGAG ATGACTCAAA TATTGAAGAA GCACAGAAAA TATTGCTAGA GACATCGTTC AAAGCTTGTA GGGGAGCAGA GGGAAAGCTG TGGGATCAGG AGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
Details for EncuA.00440.a.A1.PW27477
PURIFICATION DATe: 8/18/2009
CONCENTRATION: 24.72mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 21
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMMDCDHL LRLGMTAKKI LENGKGILAA DETPKTLGRX FEKLGITNTE ENRRKFREIL FSTKGIERYI GGVILNQETF EQTSGSGVPL TELLKKKGIE IGIKLDKGLI DYKEKEKISV GLEDLDLRCK SSAFKDATFA KWRSLFYFYD GIPSEDCINE NCSILAKYAI ICQKNGLVPI VEPEVFLEGD YSMKRSYEVT RQILSTLMKY LNYELVYIPG VLIKASYVTS GQLSNEKYTP KKVATFTLRA LLSTIPCGIP GIVFLSGGHG SEDAIGFLNA INMERGCRTW SLSFSFARAL TDGVLETWRG DDSNIEEAQK ILLETSFKAC RGAEGKLW
Validated NT Sequence
atcccacagc tttccctctg ctcccctaca agctttgaac gatgtctcta gcaatatttt ctgtgcttct tcaatatttg agtcatctcc cctccatgtt tctagaactc catctgtcaa cgccctcgca aagctaaagc tcaagctcca tgttctgcat cctctttcca tgtttattgc attgagaaag cctattgcat cttccgagcc atgtccacca ctcagaaaaa cgatgcctgg aattccacag ggaatggttg agagaagagc cctgagtgtg aaggtggcaa ccttctttgg cgtatatttt tcgttgctaa gttgtccgga cgtgacatag cttgccttaa tgagtactcc tgggatgtaa accaattcgt agtttaagta cttcattaga gtgcttaata tctgcctggt tacctcgtat gatctcttca tgctgtagtc cccttcaaga aatacctcag gctccacaat cgggacaagc ccattcttct ggcatattat tgcgtatttt gccaggatgg aacagttttc gttgatgcag tcttccgagg gaattccgtc gtagaagtag aacagagacc tccacttggc aaatgtggcg tctttgaatg ccgacgactt gcaccttagg tccaagtcct caagccctac agagatcttt tccttttcct tgtagtctat cagcccttta tcgagcttta tgcctatttc gatgcccttt ttctttagca gctctgttag tggcactcca cttccacttg tctgctcgaa tgtttcttga tttaggataa ccccgccaat gtacctttca attcccttag tgctaaatag tatttctctg aacttccttc tattctcttc ggtattcgtg atcccaagct tttcaaatnt tctgcccaac gtctttgggg tttcatcggc cgcgagaatc cctttcccgt tttccaatat cttttttgca gtcattccaa gccttaaaag atgatcacag tccatcatcg aaccaggacc ctgggtctga gcttccaggg tacccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMMDCDHLLR LGMTAKKILE NGKGILAADE TPKTLGRRFE KLGITNTEEN RRKFREILFS TKGIERYIGG VILNQETFEQ TSGSGVPLTE LLKKKGIEIG IKLDKGLIDY KEKEKISVGL EDLDLRCKSS AFKDATFAKW RSLFYFYDGI PSEDCINENC SILAKYAIIC QKNGLVPIVE PEVFLEGDYS MKRSYEVTRQ ILSTLMKYLN YELVYIPGVL IKASYVTSGQ LSNEKYTPKK VATFTLRALL STIPCGIPGI VFLSGGHGSE DAIGFLNAIN MERGCRTWSL SFSFARALTD GVLETWRGDD SNIEEAQKIL LETSFKACRG AEGKLWDQE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATGGACTGT GATCATCTTT TAAGGCTTGG AATGACTGCA AAAAAGATAT TGGAAAACGG GAAAGGGATT CTCGCGGCCG ATGAAACCCC AAAGACGTTG GGCAGAAGAT TTGAAAAGCT TGGGATCACG AATACCGAAG AGAATAGAAG GAAGTTCAGA GAAATACTAT TTAGCACTAA GGGAATTGAA AGGTACATTG GCGGGGTTAT CCTAAATCAA GAAACATTCG AGCAGACAAG TGGAAGTGGA GTGCCACTAA CAGAGCTGCT AAAGAAAAAG GGCATCGAAA TAGGCATAAA GCTCGATAAA GGGCTGATAG ACTACAAGGA AAAGGAAAAG ATCTCTGTAG GGCTTGAGGA CTTGGACCTA AGGTGCAAGT CGTCGGCATT CAAAGACGCC ACATTTGCCA AGTGGAGGTC TCTGTTCTAC TTCTACGACG GAATTCCCTC GGAAGACTGC ATCAACGAAA ACTGTTCCAT CCTGGCAAAA TACGCAATAA TATGCCAGAA GAATGGGCTT GTCCCGATTG TGGAGCCTGA GGTATTTCTT GAAGGGGACT ACAGCATGAA GAGATCATAC GAGGTAACCA GGCAGATATT AAGCACTCTA ATGAAGTACT TAAACTACGA ATTGGTTTAC ATCCCAGGAG TACTCATTAA GGCAAGCTAT GTCACGTCCG GACAACTTAG CAACGAAAAA TATACGCCAA AGAAGGTTGC CACCTTCACA CTCAGGGCTC TTCTCTCAAC CATTCCCTGT GGAATTCCAG GCATCGTTTT TCTGAGTGGT GGACATGGCT CGGAAGATGC AATAGGCTTT CTCAATGCAA TAAACATGGA AAGAGGATGC AGAACATGGA GCTTGAGCTT TAGCTTTGCG AGGGCGTTGA CAGATGGAGT TCTAGAAACA TGGAGGGGAG ATGACTCAAA TATTGAAGAA GCACAGAAAA TATTGCTAGA GACATCGTTC AAAGCTTGTA GGGGAGCAGA GGGAAAGCTG TGGGATCAGG AGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA