SSGCID: Target Page

EncuA.00411.a

Putative uncharacterized protein ECU08_1860

CENTER ID:	EncuA.00411.a
ORGANISM:	Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE:	Microsporidiosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EncuA.00411.a.A1.GE27205	Full length( EncuA.00411.a )		1	199

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	MicrosporidiaDB:ECU08_1860
RefSeq:	NP_597313.1
UniProt:	Q8SUJ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKIKELKIEL TKVRDFSKMS VGLEQYMTPP DIAASMVSVI HSTYGDIEGK SILDLCCGTG MLSFACSYFS PSYILGVDLC PVALEIFRQN SLEFQINADL LRCSIDDLIF INGRFDTAII NPPFGTKIRH ADTRAVDKAL ELCNVVYSLH KTSTREYMVK RYPGAEVLAE IRYELPRKHD FHKKDKRSVG VDFIRIERR

NT Sequence

ATGAAGATTA AGGAGCTGAA AATAGAACTT ACCAAGGTCA GAGACTTCAG CAAAATGAGT GTTGGCCTAG AGCAGTACAT GACTCCGCCA GACATAGCAG CGTCGATGGT TAGTGTTATA CATTCGACCT ATGGTGATAT TGAAGGAAAG TCCATCCTGG ACTTGTGCTG TGGCACAGGG ATGTTGTCTT TCGCATGCAG CTATTTTTCC CCTTCATACA TCCTGGGGGT TGATCTGTGT CCAGTAGCCC TAGAGATATT CAGACAGAAT AGTCTCGAGT TCCAGATCAA TGCAGATCTT TTAAGATGCT CTATCGACGA CCTTATCTTT ATCAATGGGA GATTCGACAC GGCAATAATC AATCCGCCCT TTGGGACGAA GATAAGGCAT GCAGACACAA GGGCTGTTGA CAAGGCGCTG GAGCTCTGTA ATGTAGTGTA TTCCCTTCAT AAGACAAGTA CCAGGGAGTA CATGGTCAAA AGATATCCAG GGGCAGAGGT TTTGGCAGAG ATCAGATATG AGCTCCCAAG AAAGCATGAC TTCCACAAAA AGGACAAGAG ATCTGTTGGC GTTGATTTTA TTCGAATCGA AAGACGC

Details for EncuA.00411.a.A1.GE27205

HARVESTED ON:	7/31/2009
SEQUENCED ON:	8/12/2009
EXPECTED MW:	24kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	84

Validated AA Sequence

Validated NT Sequence

accctggaag ctcagaccca gggtcctggt tcgatgaaga ttaaggagct gaaaatagaa cttaccaagg tcagagactt cagcaaaatg agtgttggcc tagagcagta catgactccg ccagacatag cagcgtcgat ggttagtgtt atacattcga cctatggtga tattgaagga aagtccatcc tggacttgtg ctgtggcaca gggatgttgt ctttcgcatg cagctatttt tccccttcat acatcctggg ggttgatctg tgtccagtag ccctagagat attcagacag aatagtctcg agttccagat caatgcagat cttttaagat gctctatcga cgaccttatc tttatcaatg ggagattcga cacggcaata atcaatccgc cctttgggac gaagataagg catgcagaca caagggctgt tgacaaggcg ctggagctct gtaatgtagt gtattccctt cataagacaa gtaccaggga gtacatggtc aaaagatatc caggggcaga ggttttggca gagatcagat atgagctccc aagaaagcat gacttccaca aaaaggacaa ga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKIKELKIE LTKVRDFSKM SVGLEQYMTP PDIAASMVSV IHSTYGDIEG KSILDLCCGT GMLSFACSYF SPSYILGVDL CPVALEIFRQ NSLEFQINAD LLRCSIDDLI FINGRFDTAI INPPFGTKIR HADTRAVDKA LELCNVVYSL HKTSTREYMV KRYPGAEVLA EIRYELPRKH DFHKKDKRSV GVDFIRIERR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagattaag gagctgaaaa tagaacttac caaggtcaga gacttcagca aaatgagtgt tggcctagag cagtacatga ctccgccaga catagcagcg tcgatggtta gtgttataca ttcgacctat ggtgatattg aaggaaagtc catcctggac ttgtgctgtg gcacagggat gttgtctttc gcatgcagct atttttcccc ttcatacatc ctgggggttg atctgtgtcc agtagcccta gagatattca gacagaatag tctcgagttc cagatcaatg cagatctttt aagatgctct atcgacgacc ttatctttat caatgggaga ttcgacacgg caataatcaa tccgcccttt gggacgaaga taaggcatgc agacacaagg gctgttgaca aggcgctgga gctctgtaat gtagtgtatt cccttcataa gacaagtacc agggagtaca tggtcaaaag atatccaggg gcagaggttt tggcagagat cagatatgag ctcccaagaa agcatgactt ccacaaaaag gacaagagat ctgttggcgt tgattttatt cgaatcgaaa gacgcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa