EncuA.00320.a

40S RIBOSOMAL PROTEIN SA or P40

CENTER ID: EncuA.00320.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00320.a.A1.GE27856 Full length( EncuA.00320.a ) 1 252
EncuA.00320.a.A1.GE28308 Full length( EncuA.00320.a ) 1 252
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU04_0450
OrthoMCL: OG5_127014
RefSeq: NP_584728.1
UniProt: Q8SS60
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPQDNTRISD SIKIPDEFVK LLIVSQSHLG GTSTNKSFAR YLYGTRPRDR INIIDINATW EKLIIAARAF CGIKHPSSIA VVSTKTFGRK PVVKFCEAVG ATPITGRFIP GSFTNSEVKR VYDPRVLIVS DTYADKQAIL ESQYCNLPTI AFVNTDNSLV GVDIAIPMNN RSPSAIAAGF FILSRLINYM KTGAELVRDM KEVELFLFRD SVELEQLVEE QLLETTDSIL NVGKEGILSG IGTGNADEWN SF
NT Sequence
ATGCCACAAG ATAACACCAG GATCTCGGAT AGCATTAAAA TACCAGACGA GTTCGTTAAG CTGCTGATAG TCAGCCAGTC GCATCTTGGA GGAACCTCCA CGAACAAGAG CTTTGCCAGG TACCTCTATG GAACTAGGCC TCGCGACAGA ATCAACATCA TTGATATCAA TGCGACATGG GAAAAGCTCA TCATTGCAGC AAGAGCTTTC TGTGGCATAA AGCACCCCTC CAGCATCGCA GTGGTGTCTA CGAAGACGTT TGGGAGAAAG CCTGTTGTCA AGTTCTGCGA AGCGGTCGGA GCAACGCCGA TAACCGGAAG ATTTATTCCG GGGTCGTTTA CAAACTCGGA AGTCAAGCGA GTATATGACC CAAGAGTCCT TATTGTTTCT GACACATATG CTGACAAGCA GGCTATCTTG GAGTCGCAAT ACTGTAACCT TCCCACGATT GCATTTGTTA ACACAGACAA TTCTTTAGTG GGGGTAGACA TTGCAATTCC CATGAACAAC AGATCTCCAT CGGCAATTGC TGCAGGGTTT TTTATCTTGT CAAGGTTAAT TAATTACATG AAGACTGGGG CTGAGCTTGT TCGAGACATG AAGGAGGTTG AGCTCTTCCT GTTTAGAGAT AGCGTCGAGC TTGAGCAGCT TGTTGAGGAG CAGCTTCTTG AGACAACAGA CTCCATTCTT AATGTTGGAA AGGAGGGGAT TTTGAGTGGG ATAGGCACTG GGAATGCCGA CGAGTGGAAC AGCTTC
Details for EncuA.00320.a.A1.GE27856
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 30kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPQDNTRIS DSIKIPDEFV KLLIVSQSHL GGTSTNKSFA RYLYGTRPRD RINIIDINAT WEKLIIAARA FCGIKHPSSI AVVSTKTFGR KPVVKFCEAV GATPITGRFI PGSFTNSEVK RVYDPRVLIV SDTYADKQAI LESQYCNLPT IAFVNTDNSL VGVDIAIPMN NRSPSAIAAG FFILSRLINY MKTGAELVRD MKEVELFLFR DSVELEQLVE EQLLETTDSI LNVGKEGILS GIGTGNADEW NSF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccac aagataacac caggatctcg gatagcatta aaataccaga cgagttcgtt aagctgctga tagtcagcca gtcgcatctt ggaggaacct ccacgaacaa gagctttgcc aggtacctct atggaactag gcctcgcgac agaatcaaca tcattgatat caatgcgaca tgggaaaagc tcatcattgc agcaagagct ttctgtggca taaagcaccc ctccagcatc gcagtggtgt ctacgaagac gtttgggaga aagcctgttg tcaagttctg cgaagcggtc ggagcaacgc cgataaccgg aagatttatt ccggggtcgt ttacaaactc ggaagtcaag cgagtatatg acccaagagt ccttattgtt tctgacacat atgctgacaa gcaggctatc ttggagtcgc aatactgtaa ccttcccacg attgcatttg ttaacacaga caattcttta gtgggggtag acattgcaat tcccatgaac aacagatctc catcggcaat tgctgcaggg ttttttatct tgtcaaggtt aattaattac atgaagactg gggctgagct tgttcgagac atgaaggagg ttgagctctt cctgtttaga gatagcgtcg agcttgagca gcttgttgag gagcagcttc ttgagacaac agactccatt cttaatgttg gaaaggaggg gattttgagt gggataggca ctgggaatgc cgacgagtgg aacagcttct aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPQDNTRIS DSIKIPDEFV KLLIVSQSHL GGTSTNKSFA RYLYGTRPRD RINIIDINAT WEKLIIAARA FCGIKHPSSI AVVSTKTFGR KPVVKFCEAV GATPITGRFI PGSFTNSEVK RVYDPRVLIV SDTYADKQAI LESQYCNLPT IAFVNTDNSL VGVDIAIPMN NRSPSAIAAG FFILSRLINY MKTGAELVRD MKEVELFLFR DSVELEQLVE EQLLETTDSI LNVGKEGILS GIGTGNADEW NSF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccacaagat aacaccagga tctcggatag cattaaaata ccagacgagt tcgttaagct gctgatagtc agccagtcgc atcttggagg aacctccacg aacaagagct ttgccaggta cctctatgga actaggcctc gcgacagaat caacatcatt gatatcaatg cgacatggga aaagctcatc attgcagcaa gagctttctg tggcataaag cacccctcca gcatcgcagt ggtgtctacg aagacgtttg ggagaaagcc tgttgtcaag ttctgcgaag cggtcggagc aacgccgata accggaagat ttattccggg gtcgtttaca aactcggaag tcaagcgagt atatgaccca agagtcctta ttgtttctga cacatatgct gacaagcagg ctatcttgga gtcgcaatac tgtaaccttc ccacgattgc atttgttaac acagacaatt ctttagtggg ggtagacatt gcaattccca tgaacaacag atctccatcg gcaattgctg cagggttttt tatcttgtca aggttaatta attacatgaa gactggggct gagcttgttc gagacatgaa ggaggttgag ctcttcctgt ttagagatag cgtcgagctt gagcagcttg ttgaggagca gcttcttgag acaacagact ccattcttaa tgttggaaag gaggggattt tgagtgggat aggcactggg aatgccgacg agtggaacag cttcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for EncuA.00320.a.A1.GE28308
HARVESTED ON: 1/5/2010
SEQUENCED ON: 1/15/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPQDNTRIS DSIKIPDEFV KLLIVSQSHL GGTSTNKSFA RYLYGTRPRD RINIIDINAT WEKLIIAARA FCGIKHPSSI AVVSTKTFGR KPVVKFCEAV GATPITGRFI PGSFTNSEVK RVYDPRVLIV SDTYADKQAI LESQYCNLPT IAFVNTDNSL VGVDIAIPMN NRSPSAIAAG FFILSRLINY MKTGAELVRD MKEVELFLFR DSVELEQLVE EQLLETTDSI LNVGKEGILS GIGTGNADEW NSF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccac aagataacac caggatctcg gatagcatta aaataccaga cgagttcgtt aagctgctga tagtcagcca gtcgcatctt ggaggaacct ccacgaacaa gagctttgcc aggtacctct atggaactag gcctcgcgac agaatcaaca tcattgatat caatgcgaca tgggaaaagc tcatcattgc agcaagagct ttctgtggca taaagcaccc ctccagcatc gcagtggtgt ctacgaagac gtttgggaga aagcctgttg tcaagttctg cgaagcggtc ggagcaacgc cgataaccgg aagatttatt ccggggtcgt ttacaaactc ggaagtcaag cgagtatatg acccaagagt ccttattgtt tctgacacat atgctgacaa gcaggctatc ttggagtcgc aatactgtaa ccttcccacg attgcatttg ttaacacaga caattcttta gtgggggtag acattgcaat tcccatgaac aacagatctc catcggcaat tgctgcaggg ttttttatct tgtcaaggtt aattaattac atgaagactg gggctgagct tgttcgagac atgaaggagg ttgagctctt cctgtttaga gatagcgtcg agcttgagca gcttgttgag gagcagcttc ttgagacaac agactccatt cttaatgttg gaaaggaggg gattttgagt gggataggca ctgggaatgc cgacgagtgg aacagcttct aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPQDNTRIS DSIKIPDEFV KLLIVSQSHL GGTSTNKSFA RYLYGTRPRD RINIIDINAT WEKLIIAARA FCGIKHPSSI AVVSTKTFGR KPVVKFCEAV GATPITGRFI PGSFTNSEVK RVYDPRVLIV SDTYADKQAI LESQYCNLPT IAFVNTDNSL VGVDIAIPMN NRSPSAIAAG FFILSRLINY MKTGAELVRD MKEVELFLFR DSVELEQLVE EQLLETTDSI LNVGKEGILS GIGTGNADEW NSF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccacaagat aacaccagga tctcggatag cattaaaata ccagacgagt tcgttaagct gctgatagtc agccagtcgc atcttggagg aacctccacg aacaagagct ttgccaggta cctctatgga actaggcctc gcgacagaat caacatcatt gatatcaatg cgacatggga aaagctcatc attgcagcaa gagctttctg tggcataaag cacccctcca gcatcgcagt ggtgtctacg aagacgtttg ggagaaagcc tgttgtcaag ttctgcgaag cggtcggagc aacgccgata accggaagat ttattccggg gtcgtttaca aactcggaag tcaagcgagt atatgaccca agagtcctta ttgtttctga cacatatgct gacaagcagg ctatcttgga gtcgcaatac tgtaaccttc ccacgattgc atttgttaac acagacaatt ctttagtggg ggtagacatt gcaattccca tgaacaacag atctccatcg gcaattgctg cagggttttt tatcttgtca aggttaatta attacatgaa gactggggct gagcttgttc gagacatgaa ggaggttgag ctcttcctgt ttagagatag cgtcgagctt gagcagcttg ttgaggagca gcttcttgag acaacagact ccattcttaa tgttggaaag gaggggattt tgagtgggat aggcactggg aatgccgacg agtggaacag cttcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa