SSGCID: Target Page

EncuA.00319.a

RAS-RELATED PROTEIN GTP-BINDING PROTEIN

CENTER ID:	EncuA.00319.a
ORGANISM:	Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE:	Microsporidiosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EncuA.00319.a.A1.GE27197	Full length( EncuA.00319.a )		1	203

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	MicrosporidiaDB:ECU09_1450
RefSeq:	XP_955698.1
UniProt:	Q8SQL3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAALRIDYRY KIIVLGESDV GKTCLLHKYK EDDFKQSLMT TIGVDTVSKS IVLNGKNIML NIWDTAGQER FFSITKSYYR NADGILLIFD ISDERTFSCV DRWFGRIREE TGNVPLFLVG NKRDKVDSAM YHEMEGRFKN KADEMRARYY TTSAKTGENV NNIFEDMAMV LLKKQTLGQK WEAGSLYDPS VGGPNRKTNG RCC

NT Sequence

ATGGCTGCCC TGAGGATAGA CTATCGCTAC AAGATAATAG TCCTTGGAGA GAGCGACGTG GGAAAGACAT GCCTTCTGCA CAAGTACAAG GAGGATGACT TCAAGCAGTC TCTGATGACA ACGATCGGGG TGGACACCGT CTCAAAAAGC ATAGTTCTGA ACGGAAAGAA TATCATGCTC AACATCTGGG ACACGGCAGG TCAAGAAAGG TTCTTTTCGA TAACAAAGAG CTACTATAGA AATGCAGACG GGATACTCCT CATCTTCGAC ATCAGCGACG AGAGGACCTT TTCATGTGTG GATAGATGGT TTGGGCGCAT AAGAGAAGAG ACAGGAAATG TCCCTCTGTT CCTTGTTGGA AACAAGAGGG ATAAGGTGGA TAGCGCTATG TACCATGAGA TGGAGGGCAG ATTCAAGAAC AAGGCAGATG AAATGCGGGC CAGGTATTAC ACGACAAGCG CCAAGACGGG TGAGAATGTA AACAATATCT TCGAGGATAT GGCAATGGTC CTCCTCAAGA AGCAGACCCT GGGGCAGAAG TGGGAGGCGG GAAGTCTGTA CGACCCGTCG GTAGGCGGGC CAAATAGAAA GACCAATGGC AGGTGCTGC

Details for EncuA.00319.a.A1.GE27197

HARVESTED ON:	7/31/2009
SEQUENCED ON:	8/12/2009
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAALRIDYR YKIIVLGESD VGKTCLLHKY KEDDFKQSLM TTIGVDTVSK SIVLNGKNIM LNIWDTAGQE RFFSITKSYY RNADGILLIF DISDERTFSC VDRWFGRIRE ETGNVPLFLV GNKRDKVDSA MYHEMEGRFK NKADEMRARY YTTSAKTGEN VNNIFEDMAM VLLKKQTLGQ KWEAGSLYDP SVGGPNRKTN GRCC

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctg ccctgaggat agactatcgc tacaagataa tagtccttgg agagagcgac gtgggaaaga catgccttct gcacaagtac aaggaggatg acttcaagca gtctctgatg acaacgatcg gggtggacac cgtctcaaaa agcatagttc tgaacggaaa gaatatcatg ctcaacatct gggacacggc aggtcaagaa aggttctttt cgataacaaa gagctactat agaaatgcag acgggatact cctcatcttc gacatcagcg acgagaggac cttttcatgt gtggatagat ggtttgggcg cataagagaa gagacaggaa atgtccctct gttccttgtt ggaaacaaga gggataaggt ggatagcgct atgtaccatg agatggaggg cagattcaag aacaaggcag atgaaatgcg ggccaggtat tacacgacaa gcgccaagac gggtgagaat gtaaacaata tcttcgagga tatggcaatg gtcctcctca agaagcagac cctggggcag aagtgggagg cgggaagtct gtacgacccg tcggtaggcg ggccaaatag aaagaccaat ggcaggtgct gctaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgccctg aggatagact atcgctacaa gataatagtc cttggagaga gcgacgtggg aaagacatgc cttctgcaca agtacaagga ggatgacttc aagcagtctc tgatgacaac gatcggggtg gacaccgtct caaaaagcat agttctgaac ggaaagaata tcatgctcaa catctgggac acggcaggtc aagaaaggtt cttttcgata acaaagagct actatagaaa tgcagacggg atactcctca tcttcgacat cagcgacgag aggacctttt catgtgtgga tagatggttt gggcgcataa gagaagagac aggaaatgtc cctctgttcc ttgttggaaa caagagggat aaggtggata gcgctatgta ccatgagatg gagggcagat tcaagaacaa ggcagatgaa atgcgggcca ggtattacac gacaagcgcc aagacgggtg agaatgtaaa caatatcttc gaggatatgg caatggtcct cctcaagaag cagaccctgg ggcagaagtg ggaggcggga agtctgtacg acccgtcggt aggcgggcca aatagaaaga ccaatggcag gtgctgcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa