EncuA.00307.a

26S protease regulatory subunit 6B homolog

CENTER ID: EncuA.00307.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00307.a.A1.GE27196 Full length( EncuA.00307.a ) 1 387
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU08_1970
OrthoMCL: OG5_127831
RefSeq: NP_586128.1
UniProt: Q8SQI9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSARLKGGLF VRYKQMERKL RLLEIREGYV RKEIETLKRE ERHSREELDR VKSVPLIMGQ FLEPIDSSTA IVGSTAGSNF VVRILSTVDR ELLKPNTTVA LHRHSSAIVG VLPPEVDSTI PVMGESEKPS VTYGDVGGLD VQKQEIKETV ELPLLQSDLY RQIGIDPPQG VLLYGPPGTG KTMLVKAVAN HTKATFIRVN GSEFVQKYLG EGPRMVRDVF RLAREKAPSI VFIDEVDSIA TKRFDASTSA DREVQRVLIE LLNQMDGFDP AANVKVIMAT NRADTIDPAL LRPGRLDRKI EFPLPDRRQK RLVFNAITSK MSLNDSVDIE SLVCRPEKIS CADINSICQE AGMLAVRASR YMVTQRDFEE AYSKVVERSG TQPAFYN
NT Sequence
ATGTCTGCAA GGCTCAAGGG CGGTCTGTTT GTCAGATACA AACAAATGGA AAGGAAGCTC AGGCTCCTGG AGATAAGAGA GGGGTATGTG CGCAAGGAGA TCGAGACTCT CAAGAGGGAG GAGAGGCACA GCAGGGAGGA GCTTGACCGG GTCAAGAGTG TTCCGCTGAT AATGGGGCAG TTTCTTGAGC CGATTGACAG CTCGACGGCG ATTGTGGGGT CGACTGCGGG AAGCAACTTT GTTGTCAGGA TTCTGTCGAC TGTGGACAGG GAGCTGCTGA AGCCAAACAC CACTGTTGCG CTGCACAGGC ACTCGAGCGC GATTGTCGGG GTGCTCCCGC CGGAGGTCGA CAGCACGATT CCAGTGATGG GGGAGTCCGA GAAGCCCTCT GTGACGTACG GAGATGTGGG GGGCCTTGAT GTGCAGAAGC AAGAGATAAA GGAGACTGTG GAGCTCCCGC TGTTGCAGTC TGACCTGTAC AGGCAGATTG GAATTGACCC TCCGCAGGGC GTTCTGCTCT ATGGGCCGCC CGGAACGGGG AAGACGATGC TTGTGAAGGC TGTGGCGAAC CACACGAAGG CCACGTTTAT CAGGGTGAAC GGGTCAGAGT TCGTGCAGAA GTATCTTGGA GAGGGCCCGC GGATGGTCAG GGATGTGTTC CGGCTGGCAA GGGAAAAGGC GCCGTCGATT GTGTTTATCG ACGAGGTGGA CTCGATTGCA ACGAAGAGGT TTGATGCATC GACCTCGGCA GACAGAGAGG TCCAGCGTGT GCTGATAGAG CTGCTGAACC AGATGGACGG GTTCGACCCG GCGGCAAATG TCAAGGTGAT TATGGCCACG AACAGGGCGG ACACGATTGA CCCTGCACTC CTCAGGCCTG GGAGGCTGGA CAGAAAGATA GAGTTTCCGC TCCCCGACAG GAGGCAGAAG AGACTTGTGT TCAATGCGAT TACATCGAAG ATGAGCCTGA ATGACTCTGT GGACATTGAA AGCCTTGTGT GCAGGCCCGA GAAGATAAGC TGTGCAGACA TCAACTCGAT CTGCCAGGAG GCGGGGATGC TTGCTGTTCG GGCCAGCAGA TACATGGTTA CGCAGAGGGA CTTTGAGGAG GCATACTCGA AGGTTGTTGA GAGGAGCGGC ACACAGCCTG CATTCTATAA T
Details for EncuA.00307.a.A1.GE27196
HARVESTED ON: 7/31/2009
SEQUENCED ON: 8/12/2009
EXPECTED MW: 45kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 87
Validated AA Sequence
MERKLRLLEI REGYVRKEIE TLKREERHSR EELDRVKSVP LIMGQFLEPI DSSTAIVGST AGSNFVVRIL STVDRELLKP NTTVALHRHS SAIVGVLPPE VDSTIPVMGE SEKPSVTYGD VGGLDVQKQE IKETVELPLL QSDLYRQIGI DPPQGVLLYG PPGTGKTMLV KAVANHTKAT FIRVNGSEFV QKYLGEGPRM VRDVFRLARE KAPSIVFIDE VDSIATKRFD ASTSADREVQ RVLIELLNQM DGFDPAANVK VIMATNRADT IDPALLRPGR LDRKIEFPLP DRRQKRLVFN AITSKMSLND SVDIESLVCR PEKISCADIN SICQEAGMLA VRASRYMVTQ RDFEE
Validated NT Sequence
caagggcggt ctgtttgtca gatacaaaca aatggaaagg aagctcaggc tcctggagat aagagagggg tatgtgcgca aggagatcga gactctcaag agggaggaga ggcacagcag ggaggagctt gaccgggtca agagtgttcc gctgataatg gggcagtttc ttgagccgat tgacagctcg acggcgattg tggggtcgac tgcgggaagc aactttgttg tcaggattct gtcgactgtg gacagggagc tgctgaagcc aaacaccact gttgcgctgc acaggcactc gagcgcgatt gtcggggtgc tcccgccgga ggtcgacagc acgattccag tgatggggga gtccgagaag ccctctgtga cgtacggaga tgtggggggc cttgatgtgc agaagcaaga gataaaggag actgtggagc tcccgctgtt gcagtctgac ctgtacaggc agattggaat tgaccctccg cagggcgttc tgctctatgg gccgcccgga acggggaaga cgatgcttgt gaaggctgtg gcgaaccaca cgaaggccac gtttatcagg gtgaacgggt cagagttcgt gcagaagtat cttggagagg gcccgcggat ggtcagggat gtgttccggc tggcaaggga aaaggcgccg tcgattgtgt ttatcgacga ggtggactcg attgcaacga agaggtttga tgcatcgacc tcggcagaca gagaggtcca gcgtgtgctg atagagctgc tgaaccagat ggacgggttc gacccggcgg caaatgtcaa ggtgattatg gccacgaaca gggcggacac gattgaccct gcactcctca ggcctgggag gctggacaga aagatagagt ttccgctccc cgacaggagg cagaagagac ttgtgttcaa tgcgattaca tcgaagatga gcctgaatga ctctgtggac attgaaagcc ttgtgtgcag gcccgagaag ataagctgtg cagacatcaa ctcgatctgc caggaggcgg ggatgcttgc tgttcgggcc agcagataca tggttacgca gagggacttt gaggaggc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSARLKGGL FVRYKQMERK LRLLEIREGY VRKEIETLKR EERHSREELD RVKSVPLIMG QFLEPIDSST AIVGSTAGSN FVVRILSTVD RELLKPNTTV ALHRHSSAIV GVLPPEVDST IPVMGESEKP SVTYGDVGGL DVQKQEIKET VELPLLQSDL YRQIGIDPPQ GVLLYGPPGT GKTMLVKAVA NHTKATFIRV NGSEFVQKYL GEGPRMVRDV FRLAREKAPS IVFIDEVDSI ATKRFDASTS ADREVQRVLI ELLNQMDGFD PAANVKVIMA TNRADTIDPA LLRPGRLDRK IEFPLPDRRQ KRLVFNAITS KMSLNDSVDI ESLVCRPEKI SCADINSICQ EAGMLAVRAS RYMVTQRDFE EAYSKVVERS GTQPAFYN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctgcaagg ctcaagggcg gtctgtttgt cagatacaaa caaatggaaa ggaagctcag gctcctggag ataagagagg ggtatgtgcg caaggagatc gagactctca agagggagga gaggcacagc agggaggagc ttgaccgggt caagagtgtt ccgctgataa tggggcagtt tcttgagccg attgacagct cgacggcgat tgtggggtcg actgcgggaa gcaactttgt tgtcaggatt ctgtcgactg tggacaggga gctgctgaag ccaaacacca ctgttgcgct gcacaggcac tcgagcgcga ttgtcggggt gctcccgccg gaggtcgaca gcacgattcc agtgatgggg gagtccgaga agccctctgt gacgtacgga gatgtggggg gccttgatgt gcagaagcaa gagataaagg agactgtgga gctcccgctg ttgcagtctg acctgtacag gcagattgga attgaccctc cgcagggcgt tctgctctat gggccgcccg gaacggggaa gacgatgctt gtgaaggctg tggcgaacca cacgaaggcc acgtttatca gggtgaacgg gtcagagttc gtgcagaagt atcttggaga gggcccgcgg atggtcaggg atgtgttccg gctggcaagg gaaaaggcgc cgtcgattgt gtttatcgac gaggtggact cgattgcaac gaagaggttt gatgcatcga cctcggcaga cagagaggtc cagcgtgtgc tgatagagct gctgaaccag atggacgggt tcgacccggc ggcaaatgtc aaggtgatta tggccacgaa cagggcggac acgattgacc ctgcactcct caggcctggg aggctggaca gaaagataga gtttccgctc cccgacagga ggcagaagag acttgtgttc aatgcgatta catcgaagat gagcctgaat gactctgtgg acattgaaag ccttgtgtgc aggcccgaga agataagctg tgcagacatc aactcgatct gccaggaggc ggggatgctt gctgttcggg ccagcagata catggttacg cagagggact ttgaggaggc atactcgaag gttgttgaga ggagcggcac acagcctgca ttctataata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a