EncuA.00253.a

ATP-dependent RNA helicase eIF4A

CENTER ID: EncuA.00253.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00253.a.A1.PW28202 Full length( EncuA.00253.a ) 1 425
External Resources
RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU09_1200
OrthoMCL: OG5_126984
RefSeq: XP_955671.1
UniProt: Q8SQM5
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKQVTEQAED FVDTRSSGTE IREFEDLRSD SSQIRMFDTW EDYGLKEDLL KGIYSIGFET PSFIQKAAIQ PIIDGRDIRA QAQSGTGKTG AFAVAALQIC DMSQDVTQIL VLASTREIAA QNAARFEDLG CFMGARVALL SGGSPIAADK VALEKKPHIV VGTPGRVEHM ININELSMDN IKLFVIDEAD EMLKAGFQEQ VKSIFRRITN KDEVQIAMFS ATYDEEELRV SEEILINPVI IDLRYNDQTL KGIRQYFIDL RKEPPFRKGR EDYLLPKLVT LYDIFRKQRL GQSIVFINSK EDARIVYDWL IRHEWECELI SAELTQAERE RTLNRFRGGT GRCLISSGLL SRGIDIQNLS VVFCLDVPSF ERKSTYIHRI GRSGRYGRKG IAINIVYEHE LKNLKAIERF YNTTIKELPA DFSFQ
NT Sequence
ATGAAACAAG TAACAGAACA AGCAGAAGAC TTTGTGGATA CGAGGAGCAG TGGTACGGAG ATCAGGGAGT TTGAGGATCT CCGGAGCGAC TCATCTCAGA TCAGGATGTT TGACACATGG GAGGACTACG GGCTGAAGGA GGATCTACTG AAGGGGATCT ACAGCATAGG ATTTGAGACG CCTTCCTTTA TTCAGAAGGC GGCGATCCAG CCGATAATAG ACGGGAGAGA CATAAGAGCG CAGGCACAGA GTGGTACTGG GAAGACTGGG GCATTTGCGG TTGCGGCGCT TCAGATATGC GATATGAGCC AGGACGTGAC ACAGATTCTT GTTCTTGCAT CGACCAGGGA GATTGCAGCA CAGAATGCAG CGAGGTTTGA GGATCTTGGA TGTTTTATGG GGGCAAGGGT CGCCCTTCTT TCTGGAGGAA GCCCGATTGC AGCAGACAAG GTTGCACTTG AAAAGAAGCC GCACATTGTT GTTGGGACGC CCGGGAGGGT TGAGCACATG ATCAATATAA ATGAATTGAG CATGGACAAC ATTAAGTTAT TTGTGATTGA TGAGGCAGAC GAGATGCTGA AGGCCGGATT CCAGGAGCAG GTGAAGTCTA TATTCCGAAG GATCACAAAC AAGGACGAGG TGCAGATAGC AATGTTCTCT GCAACATACG ACGAGGAGGA GCTCCGTGTC AGTGAGGAGA TTCTTATCAA CCCTGTGATA ATTGACCTGA GATACAACGA CCAGACCCTG AAGGGAATAA GACAGTACTT CATTGATCTG AGGAAGGAGC CTCCATTCCG GAAGGGAAGA GAGGACTATC TGCTTCCAAA GCTTGTGACG CTGTATGACA TCTTCCGCAA GCAACGCCTG GGGCAATCGA TTGTGTTTAT CAACAGCAAG GAGGATGCCA GGATAGTGTA TGACTGGCTG ATCAGGCACG AGTGGGAGTG TGAGCTTATA TCTGCAGAAC TAACACAGGC GGAGAGGGAG AGGACGCTGA ATAGGTTTCG AGGAGGGACT GGCAGATGCC TGATCTCCTC TGGACTGCTT TCAAGGGGAA TCGACATCCA GAATCTCTCT GTGGTGTTCT GCCTGGATGT TCCATCCTTC GAGAGAAAGA GCACCTATAT CCACAGGATA GGACGATCCG GAAGATATGG GAGAAAGGGA ATAGCCATAA ACATTGTCTA TGAGCATGAG CTGAAAAACC TGAAGGCGAT CGAGAGGTTC TACAACACCA CGATCAAGGA GCTTCCTGCA GATTTTAGTT TTCAA
Details for EncuA.00253.a.A1.PW28202
PURIFICATION DATe: 4/5/2010
CONCENTRATION: 28.62mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 19
VIAL VOLUME: 100µl
PERCENT IDENTITY: 76
PERCENT COVERAGE: 87
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKQVTEQAE DFVDTRSSGT EIREFEDLRS DSSQIRMFDT WEDYGLKEDL LKGIYSIGFE TPSFIQKAAI QPIIDGRDIR AQAQSGTGKT GAFAVAALQI CDMSQDVTQI LVLASTREIA AQNAARFEDL GCFMGARVAL LSGGSPIAAD KVALEKKPHI VVGTPGRVEH MININELSMD NIKLFVIDEA DEMLKAGFQE QVKSIFRRIT NKDEVQIAMF SATYDEEELR VSEEILINPV IIDLRYNDQT LKGIRQYFID LRXEPPFRKG REDYLLPKLG RCMTSSASNX WGNRXXXXQX XXQXXXXLXD XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
aattttgnnn nntttaagaa ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgaaa caagtaacag aacaagcaga agactttgtg gatacgagga gcagtggtac ggagatcagg gagtttgagg atctccggag cgactcatct cagatcagga tgtttgacac atgggaggac tacgggctga aggaggatct actgaagggg atctacagca taggatttga gacgccttcc tttattcaga aggcggcgat ccagccgata atagacggga gagacataag agcgcaggca cagagtggta ctgggaagac tggggcattt gcggttgcgg cgcttcagat atgcgatatg agccaggacg tgacacagat tcttgttctt gcatcgacca gggagattgc agcacagaat gcagcgaggt ttgaggatct tggatgtttt atgggggcaa gggtcgccct tctttctgga ggaagcccga ttgcagcaga caaggttgca cttgaaaaga agccgcacat tgttgttggg acgcccggga gggttgagca catgatcaat ataaatgaat tgagcatgga caacattaag ttatttgtga ttgatgaggc agacgagatg ctgaaggccg gattccagga gcaggtgaag tctatattcc gaaggatcac aaacaaggac gaggtgcaga tagcaatgtt ctctgcaaca tacgacgagg aggagctccg tgtcagtgag gagattctta tcaaccctgt gataattgac ctgagataca acgaccagac cctgaaggga ataagacagt acttcattga tctgaggang gagcctccnt tccggaaggg aagagaggac tatctgcttc caaagcttgg acgntgtatg acntcntccg caagcaacgn ctggggcaat cgatngngtt nnnancagcn nnnnngccag natagngnnn gactggntga tcngnncgan tngnanngnn nanctnnnnn nntgnnnant annnnnnnnn nnngnnnnnt nannnntncn nnnnngnnnn nnnatncnga nnnnnnngnn tnntnnnngn nnnnnnnnnn nannnnnnnn nnnntnnnnn ngnnnnnnnn nnnnnnnnnn nnnncnnnnn nnnnnnnnnn nnnnc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKQVTEQAE DFVDTRSSGT EIREFEDLRS DSSQIRMFDT WEDYGLKEDL LKGIYSIGFE TPSFIQKAAI QPIIDGRDIR AQAQSGTGKT GAFAVAALQI CDMSQDVTQI LVLASTREIA AQNAARFEDL GCFMGARVAL LSGGSPIAAD KVALEKKPHI VVGTPGRVEH MININELSMD NIKLFVIDEA DEMLKAGFQE QVKSIFRRIT NKDEVQIAMF SATYDEEELR VSEEILINPV IIDLRYNDQT LKGIRQYFID LRKEPPFRKG REDYLLPKLV TLYDIFRKQR LGQSIVFINS KEDARIVYDW LIRHEWECEL ISAELTQAER ERTLNRFRGG TGRCLISSGL LSRGIDIQNL SVVFCLDVPS FERKSTYIHR IGRSGRYGRK GIAINIVYEH ELKNLKAIER FYNTTIKELP ADFSFQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAACAAGTA ACAGAACAAG CAGAAGACTT TGTGGATACG AGGAGCAGTG GTACGGAGAT CAGGGAGTTT GAGGATCTCC GGAGCGACTC ATCTCAGATC AGGATGTTTG ACACATGGGA GGACTACGGG CTGAAGGAGG ATCTACTGAA GGGGATCTAC AGCATAGGAT TTGAGACGCC TTCCTTTATT CAGAAGGCGG CGATCCAGCC GATAATAGAC GGGAGAGACA TAAGAGCGCA GGCACAGAGT GGTACTGGGA AGACTGGGGC ATTTGCGGTT GCGGCGCTTC AGATATGCGA TATGAGCCAG GACGTGACAC AGATTCTTGT TCTTGCATCG ACCAGGGAGA TTGCAGCACA GAATGCAGCG AGGTTTGAGG ATCTTGGATG TTTTATGGGG GCAAGGGTCG CCCTTCTTTC TGGAGGAAGC CCGATTGCAG CAGACAAGGT TGCACTTGAA AAGAAGCCGC ACATTGTTGT TGGGACGCCC GGGAGGGTTG AGCACATGAT CAATATAAAT GAATTGAGCA TGGACAACAT TAAGTTATTT GTGATTGATG AGGCAGACGA GATGCTGAAG GCCGGATTCC AGGAGCAGGT GAAGTCTATA TTCCGAAGGA TCACAAACAA GGACGAGGTG CAGATAGCAA TGTTCTCTGC AACATACGAC GAGGAGGAGC TCCGTGTCAG TGAGGAGATT CTTATCAACC CTGTGATAAT TGACCTGAGA TACAACGACC AGACCCTGAA GGGAATAAGA CAGTACTTCA TTGATCTGAG GAAGGAGCCT CCATTCCGGA AGGGAAGAGA GGACTATCTG CTTCCAAAGC TTGTGACGCT GTATGACATC TTCCGCAAGC AACGCCTGGG GCAATCGATT GTGTTTATCA ACAGCAAGGA GGATGCCAGG ATAGTGTATG ACTGGCTGAT CAGGCACGAG TGGGAGTGTG AGCTTATATC TGCAGAACTA ACACAGGCGG AGAGGGAGAG GACGCTGAAT AGGTTTCGAG GAGGGACTGG CAGATGCCTG ATCTCCTCTG GACTGCTTTC AAGGGGAATC GACATCCAGA ATCTCTCTGT GGTGTTCTGC CTGGATGTTC CATCCTTCGA GAGAAAGAGC ACCTATATCC ACAGGATAGG ACGATCCGGA AGATATGGGA GAAAGGGAAT AGCCATAAAC ATTGTCTATG AGCATGAGCT GAAAAACCTG AAGGCGATCG AGAGGTTCTA CAACACCACG ATCAAGGAGC TTCCTGCAGA TTTTAGTTTT CAAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA