EncuA.00240.a

Uracil-DNA glycosylase

CENTER ID: EncuA.00240.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00240.a.A1.GE27195 Full length( EncuA.00240.a ) 1 286
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00240.a.A1.PW27184 Full length( EncuA.00240.a ) 1 286

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU10_0530
RefSeq: NP_586168.1
UniProt: Q8SR60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTMDREKKED LPGVFSVSES KGCHGKRQLS LDDMFSKRKT SAGTGPSKSL EAGWTYEGCA QPCEYCRLEE KIDPRWRMHL KPEFGKDYFR GVKRFLHENR NHLPPIDKIF TFSRFFPLEK TKVVIMGQDP YHNDNQAMGL SFSVPVGIPV PPSLKNIYLE ISSDIEGFEV PSHGDLSGWA ERGVLLLNDV LTVTKNRPNS HAGIGWREFT SCILQVINEK CTNVVFMLWG RHAQAKGKFI NRNKHLVLTC GHPSPLSSSH FFGCKHFSKA NKYLMDHGKS PVEWQV
NT Sequence
ATGACAATGG ATCGAGAGAA GAAAGAAGAT CTTCCTGGGG TCTTTTCCGT TTCTGAGAGC AAGGGATGCC ATGGAAAGAG GCAGCTAAGC CTAGACGATA TGTTTTCCAA AAGGAAGACT TCAGCAGGCA CAGGCCCAAG TAAGAGCTTG GAAGCAGGTT GGACCTACGA AGGATGTGCG CAGCCATGCG AATACTGTAG GCTTGAAGAG AAGATCGACC CGAGGTGGAG GATGCATCTG AAGCCTGAGT TTGGAAAGGA TTACTTCAGG GGAGTCAAGA GATTTCTCCA TGAAAACAGA AACCATTTGC CGCCCATCGA CAAGATATTT ACATTCTCGA GATTTTTCCC GCTGGAGAAG ACGAAGGTTG TTATAATGGG TCAAGACCCT TACCACAACG ATAACCAGGC GATGGGGCTG TCATTTTCTG TTCCGGTGGG GATACCTGTC CCGCCCAGTC TCAAGAACAT ATACTTGGAG ATTAGCTCTG ACATCGAGGG ATTTGAGGTT CCTTCCCATG GGGATCTGTC GGGATGGGCA GAAAGGGGAG TTCTGTTACT GAACGATGTT CTCACTGTCA CGAAGAACCG ACCAAACTCG CATGCCGGCA TCGGATGGAG GGAGTTCACA AGCTGTATTC TTCAGGTTAT AAACGAGAAA TGCACTAATG TTGTGTTCAT GCTGTGGGGG CGGCATGCAC AGGCAAAGGG CAAGTTCATC AACAGGAACA AACACCTGGT GCTAACCTGC GGACATCCAA GCCCGCTTAG CTCTAGCCAC TTCTTCGGCT GCAAGCATTT CTCAAAAGCC AACAAGTATC TCATGGACCA CGGCAAATCA CCAGTCGAGT GGCAAGTG
Details for EncuA.00240.a.A1.GE27195
HARVESTED ON: 7/31/2009
SEQUENCED ON: 8/12/2009
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMTMDREK KEDLPGVFSV SESKGCHGKR QLSLDDMFSK RKTSAGTGPS KSLEAGWTYE GCAQPCEYCR LEEKIDPRWR MHLKPEFGKD YFRGVKRFLH ENRNHLPPID KIFTFSRFFP LEKTKVVIMG QDPYHNDNQA MGLSFSVPVG IPVPPSLKNI YLEISSDIEG FEVPSHGDLS GWAERGVLLL NDVLTVTKNR PNSHAGIGWR EFTSCILQVI NEKCTNVVFM LWGRHAQAKG KFINRNKHLV LTCGHPSPLS SSHFFGCKHF SKANKYLMDH GKSPVE
Validated NT Sequence
ccactcgact ggtgatttgc cgtggtccat gagatacttg ttggcttttg agaaatgctt gcagccgaag aagtggctag agctaagcgg gcttggatgt ccgcaggtta gcaccaggtg tttgttcctg ttgatgaact tgccctttgc ctgtgcatgc cgcccccaca gcatgaacac aacattagtg catttctcgt ttataacctg aagaatacag cttgtgaact ccctccatcc gatgccggca tgcgagtttg gtcggttctt cgtgacagtg agaacatcgt tcagtaacag aactcccctt tctgcccatc ccgacagatc cccatgggaa ggaacctcaa atccctcgat gtcagagcta atctccaagt atatgttctt gagactgggc gggacaggta tccccaccgg aacagaaaat gacagcccca tcgcctggtt atcgttgtgg taagggtctt gacccattat aacaaccttc gtcttctcca gcgggaaaaa tctcgagaat gtaaatatct tgtcgatggg cggcaaatgg tttctgtttt catggagaaa tctcttgact cccctgaagt aatcctttcc aaactcaggc ttcagatgca tcctccacct cgggtcgatc ttctcttcaa gcctacagta ttcgcatggc tgcgcacatc cttcgtaggt ccaacctgct tccaagctct tacttgggcc tgtgcctgct gaagtcttcc ttttggaaaa catatcgtct aggcttagct gcctctttcc atggcatccc ttgctctcag aaacggaaaa gaccccagga agatcttctt tcttctctcg atccattgtc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTMDREKKE DLPGVFSVSE SKGCHGKRQL SLDDMFSKRK TSAGTGPSKS LEAGWTYEGC AQPCEYCRLE EKIDPRWRMH LKPEFGKDYF RGVKRFLHEN RNHLPPIDKI FTFSRFFPLE KTKVVIMGQD PYHNDNQAMG LSFSVPVGIP VPPSLKNIYL EISSDIEGFE VPSHGDLSGW AERGVLLLND VLTVTKNRPN SHAGIGWREF TSCILQVINE KCTNVVFMLW GRHAQAKGKF INRNKHLVLT CGHPSPLSSS HFFGCKHFSK ANKYLMDHGK SPVEWQV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaatggat cgagagaaga aagaagatct tcctggggtc ttttccgttt ctgagagcaa gggatgccat ggaaagaggc agctaagcct agacgatatg ttttccaaaa ggaagacttc agcaggcaca ggcccaagta agagcttgga agcaggttgg acctacgaag gatgtgcgca gccatgcgaa tactgtaggc ttgaagagaa gatcgacccg aggtggagga tgcatctgaa gcctgagttt ggaaaggatt acttcagggg agtcaagaga tttctccatg aaaacagaaa ccatttgccg cccatcgaca agatatttac attctcgaga tttttcccgc tggagaagac gaaggttgtt ataatgggtc aagaccctta ccacaacgat aaccaggcga tggggctgtc attttctgtt ccggtgggga tacctgtccc gcccagtctc aagaacatat acttggagat tagctctgac atcgagggat ttgaggttcc ttcccatggg gatctgtcgg gatgggcaga aaggggagtt ctgttactga acgatgttct cactgtcacg aagaaccgac caaactcgca tgccggcatc ggatggaggg agttcacaag ctgtattctt caggttataa acgagaaatg cactaatgtt gtgttcatgc tgtgggggcg gcatgcacag gcaaagggca agttcatcaa caggaacaaa cacctggtgc taacctgcgg acatccaagc ccgcttagct ctagccactt cttcggctgc aagcatttct caaaagccaa caagtatctc atggaccacg gcaaatcacc agtcgagtgg caagtgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for EncuA.00240.a.A1.PW27184
PURIFICATION DATe: 8/11/2009
CONCENTRATION: 25.98mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMTMDREK KEDLPGVFSV SESKGCHGKR QLSLDDMFSK RKTSAGTGPS KSLEAGWTYE GCAQPCEYCR LEEKIDPRWR MHLKPEFGKD YFRGVKRFLH ENRNHLPPID KIFTFSRFFP LEKTKVVIMG QDPYHNDNQA MGLSFSVPVG IPVPPSLKNI YLEISSDIEG FEVPSHGDLS GWAERGVLLL NDVLTVTKNR PNSHAGIGWR EFTSCILQVI NEKCTNVVFM LWGRHAQAKG KFINRNKHLV LTCGHPSPLS SSHFFGCKHF SKANKYLMDH GKSPVE
Validated NT Sequence
ccactcgact ggtgatttgc cgtggtccat gagatacttg ttggcttttg agaaatgctt gcagccgaag aagtggctag agctaagcgg gcttggatgt ccgcaggtta gcaccaggtg tttgttcctg ttgatgaact tgccctttgc ctgtgcatgc cgcccccaca gcatgaacac aacattagtg catttctcgt ttataacctg aagaatacag cttgtgaact ccctccatcc gatgccggca tgcgagtttg gtcggttctt cgtgacagtg agaacatcgt tcagtaacag aactcccctt tctgcccatc ccgacagatc cccatgggaa ggaacctcaa atccctcgat gtcagagcta atctccaagt atatgttctt gagactgggc gggacaggta tccccaccgg aacagaaaat gacagcccca tcgcctggtt atcgttgtgg taagggtctt gacccattat aacaaccttc gtcttctcca gcgggaaaaa tctcgagaat gtaaatatct tgtcgatggg cggcaaatgg tttctgtttt catggagaaa tctcttgact cccctgaagt aatcctttcc aaactcaggc ttcagatgca tcctccacct cgggtcgatc ttctcttcaa gcctacagta ttcgcatggc tgcgcacatc cttcgtaggt ccaacctgct tccaagctct tacttgggcc tgtgcctgct gaagtcttcc ttttggaaaa catatcgtct aggcttagct gcctctttcc atggcatccc ttgctctcag aaacggaaaa gaccccagga agatcttctt tcttctctcg atccattgtc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTMDREKKE DLPGVFSVSE SKGCHGKRQL SLDDMFSKRK TSAGTGPSKS LEAGWTYEGC AQPCEYCRLE EKIDPRWRMH LKPEFGKDYF RGVKRFLHEN RNHLPPIDKI FTFSRFFPLE KTKVVIMGQD PYHNDNQAMG LSFSVPVGIP VPPSLKNIYL EISSDIEGFE VPSHGDLSGW AERGVLLLND VLTVTKNRPN SHAGIGWREF TSCILQVINE KCTNVVFMLW GRHAQAKGKF INRNKHLVLT CGHPSPLSSS HFFGCKHFSK ANKYLMDHGK SPVEWQV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAATGGAT CGAGAGAAGA AAGAAGATCT TCCTGGGGTC TTTTCCGTTT CTGAGAGCAA GGGATGCCAT GGAAAGAGGC AGCTAAGCCT AGACGATATG TTTTCCAAAA GGAAGACTTC AGCAGGCACA GGCCCAAGTA AGAGCTTGGA AGCAGGTTGG ACCTACGAAG GATGTGCGCA GCCATGCGAA TACTGTAGGC TTGAAGAGAA GATCGACCCG AGGTGGAGGA TGCATCTGAA GCCTGAGTTT GGAAAGGATT ACTTCAGGGG AGTCAAGAGA TTTCTCCATG AAAACAGAAA CCATTTGCCG CCCATCGACA AGATATTTAC ATTCTCGAGA TTTTTCCCGC TGGAGAAGAC GAAGGTTGTT ATAATGGGTC AAGACCCTTA CCACAACGAT AACCAGGCGA TGGGGCTGTC ATTTTCTGTT CCGGTGGGGA TACCTGTCCC GCCCAGTCTC AAGAACATAT ACTTGGAGAT TAGCTCTGAC ATCGAGGGAT TTGAGGTTCC TTCCCATGGG GATCTGTCGG GATGGGCAGA AAGGGGAGTT CTGTTACTGA ACGATGTTCT CACTGTCACG AAGAACCGAC CAAACTCGCA TGCCGGCATC GGATGGAGGG AGTTCACAAG CTGTATTCTT CAGGTTATAA ACGAGAAATG CACTAATGTT GTGTTCATGC TGTGGGGGCG GCATGCACAG GCAAAGGGCA AGTTCATCAA CAGGAACAAA CACCTGGTGC TAACCTGCGG ACATCCAAGC CCGCTTAGCT CTAGCCACTT CTTCGGCTGC AAGCATTTCT CAAAAGCCAA CAAGTATCTC ATGGACCACG GCAAATCACC AGTCGAGTGG CAAGTGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA