EncuA.00234.a

20S PROTEASOME ALPHA-TYPE SUBUNIT

CENTER ID:	EncuA.00234.a
ORGANISM:	Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE:	Microsporidiosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EncuA.00234.a.A1.GE27190	Full length( EncuA.00234.a )		1	290

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	MicrosporidiaDB:ECU07_1420
OrthoMCL:	OG5_127834
RefSeq:	NP_586070.1
UniProt:	Q8SRH6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLSNKRGIHS ASGGIDLRTL REYCLFEDFD VCMPFNPMGV PGGKGNRGSD GAIRGMLPLG DAMGDLFTAG KAGSTKMEDK ERFDPYEDNS GTTICLKQGD FIVVAGDTRH SSSMVINSRE MSKIFQVGDF LLTGTGFYAD THEVYVKMVY EIRQYEVDDS INIHSAANLL SKILYSKRFF PYYSFCVLSG FEKGKPYVYS YDPIGSFGSV TCVCSGSGRS MIQPLLDSFI DKKNWNNAEE TQLSQEDCIR LVVKAFNSAA ERDVKTKDNL EVCVMRENQV MRETFPLRRD

NT Sequence

ATGCTGAGCA ACAAGAGAGG GATACATTCG GCGAGTGGAG GCATTGACCT GAGAACGCTG AGGGAGTACT GCCTGTTTGA GGATTTTGAT GTGTGCATGC CCTTCAATCC CATGGGCGTC CCGGGCGGCA AGGGGAACAG GGGCAGCGAT GGAGCGATCC GGGGAATGCT ACCTCTAGGA GACGCCATGG GAGATTTGTT TACTGCTGGA AAGGCTGGTT CAACAAAGAT GGAGGACAAG GAGAGATTTG ATCCATATGA AGACAACTCC GGGACAACTA TCTGCCTAAA GCAGGGCGAT TTTATTGTTG TTGCAGGAGA CACAAGGCAC TCGTCGAGCA TGGTTATCAA CTCCCGGGAG ATGTCCAAGA TATTTCAGGT CGGAGACTTT CTTCTTACCG GAACCGGATT CTATGCAGAC ACCCACGAGG TCTACGTGAA GATGGTGTAT GAAATACGGC AGTACGAGGT TGACGATTCA ATCAACATTC ACAGCGCAGC AAACCTGCTC TCAAAGATTC TGTACTCGAA GAGGTTTTTC CCATACTACT CGTTTTGTGT TCTAAGCGGG TTTGAGAAGG GAAAACCCTA TGTCTATTCA TACGATCCTA TCGGGTCCTT TGGGTCTGTG ACGTGTGTAT GTAGCGGAAG CGGTAGAAGC ATGATTCAAC CCCTGTTGGA CTCTTTCATC GACAAGAAGA ACTGGAACAA TGCAGAGGAG ACACAGCTGA GCCAGGAGGA CTGCATAAGA CTTGTTGTTA AGGCATTCAA TTCTGCAGCA GAAAGGGACG TGAAGACGAA GGACAATCTT GAGGTCTGTG TGATGAGAGA AAACCAAGTG ATGCGTGAGA CTTTCCCTCT AAGAAGAGAT

Details for EncuA.00234.a.A1.GE27190

HARVESTED ON:	7/31/2009
SEQUENCED ON:	8/12/2009
EXPECTED MW:	34kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLSNKRGIH SASGGIDLRT LREYCLFEDF DVCMPFNPMG VPGGKGNRGS DGAIRGMLPL GDAMGDLFTA GKAGSTKMED KERFDPYEDN SGTTICLKQG DFIVVAGDTR HSSSMVINSR EMSKIFQVGD FLLTGTGFYA DTHEVYVKMV YEIRQYEVDD SINIHSAANL LSKILYSKRF FPYYSFCVLS GFEKGKPYVY SYDPIGSFGS VTCVCSGSGR SMIQPLLDSF IDKKNWNNAE ETQLSQEDCI RLVVKAFNSA AERDVKTKDN LEVCVMRENQ VMRETFPLRR D

Validated NT Sequence

aatctcttct tagagggaaa gtctcacgca tcacttggtt ttctctcatc acacagacct caagattgtc cttcgtcttc acgtcccttt ctgctgcaga attgaatgcc ttaacaacaa gtcttatgca gtcctcctgg ctcagctgtg tctcctctgc attgttccag ttcttcttgt cgatgaaaga gtccaacagg ggttgaatca tgcttctacc gcttccgcta catacacacg tcacagaccc aaaggacccg ataggatcgt atgaatagac atagggtttt cccttctcaa acccgcttag aacacaaaac gagtagtatg ggaaaaacct cttcgagtac agaatctttg agagcaggtt tgctgcgctg tgaatgttga ttgaatcgtc aacctcgtac tgccgtattt catacaccat cttcacgtag acctcgtggg tgtctgcata gaatccggtt ccggtaagaa gaaagtctcc gacctgaaat atcttggaca tctcccggga gttgataacc atgctcgacg agtgccttgt gtctcctgca acaacaataa aatcgccctg ctttaggcag atagttgtcc cggagttgtc ttcatatgga tcaaatctct ccttgtcctc catctttgtt gaaccagcct ttccagcagt aaacaaatct cccatggcgt ctcctagagg tagcattccc cggatcgctc catcgctgcc cctgttcccc ttgccgcccg ggacgcccat gggattgaag ggcatgcaca catcaaaatc ctcaaacagg cagtactccc tcagcgttct caggtcaatg cctccactcg ccgaatgtat ccctctcttg ttgctcagca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgagcaac aagagaggga tacattcggc gagtggaggc attgacctga gaacgctgag ggagtactgc ctgtttgagg attttgatgt gtgcatgccc ttcaatccca tgggcgtccc gggcggcaag gggaacaggg gcagcgatgg agcgatccgg ggaatgctac ctctaggaga cgccatggga gatttgttta ctgctggaaa ggctggttca acaaagatgg aggacaagga gagatttgat ccatatgaag acaactccgg gacaactatc tgcctaaagc agggcgattt tattgttgtt gcaggagaca caaggcactc gtcgagcatg gttatcaact cccgggagat gtccaagata tttcaggtcg gagactttct tcttaccgga accggattct atgcagacac ccacgaggtc tacgtgaaga tggtgtatga aatacggcag tacgaggttg acgattcaat caacattcac agcgcagcaa acctgctctc aaagattctg tactcgaaga ggtttttccc atactactcg ttttgtgttc taagcgggtt tgagaaggga aaaccctatg tctattcata cgatcctatc gggtcctttg ggtctgtgac gtgtgtatgt agcggaagcg gtagaagcat gattcaaccc ctgttggact ctttcatcga caagaagaac tggaacaatg cagaggagac acagctgagc caggaggact gcataagact tgttgttaag gcattcaatt ctgcagcaga aagggacgtg aagacgaagg acaatcttga ggtctgtgtg atgagagaaa accaagtgat gcgtgagact ttccctctaa gaagagataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa