EncuA.00196.a

Serine/threonine protein phosphatase

CENTER ID: EncuA.00196.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00196.a.A1.GE27198 Full length( EncuA.00196.a ) 1 301
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU10_1280
RefSeq: NP_586243.1
UniProt: Q8SR19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKDHAEPKY LEILRRIKNS ILPARDEVLE LVGEVRKILE QEPNVLHIDT PVNVVGDVHG HFYDVLNMLD LMEDPSEKPY LFLGDYVDRG YNSVDLVLLL FVYKRLYRDR VHLLRGNHET RMLSSVYGFK EECMRKYDLV VYWRICETFQ YLPIAAVVLE KYFCVHGGMV PGMSLDFIMK HDRIGEITEL NDVLWSDPDE TPGFQKSQRG AGYLFGEDVL KSFLDEHGLS HFVRSHQLVN DGYKAYFDEK LYTVWGAPNY CYSSGNIACV MAIDEDGHDF RLFDRCEEQF KEAPVRHSFF D
NT Sequence
ATGACAAAGG ACCACGCTGA GCCAAAGTAT CTGGAAATTC TTCGAAGGAT CAAGAACTCC ATTCTTCCTG CAAGAGACGA GGTTCTGGAG TTGGTAGGGG AGGTCAGGAA GATACTCGAA CAGGAACCTA ATGTTCTGCA CATAGATACG CCGGTTAACG TTGTCGGAGA TGTGCATGGC CACTTCTATG ATGTACTGAA CATGCTGGAC CTGATGGAGG ATCCGTCAGA AAAGCCATAC CTATTCCTTG GAGACTATGT CGACAGGGGA TACAACTCTG TTGACCTTGT TCTTCTCCTG TTTGTATACA AGAGACTCTA TAGAGACAGG GTGCATCTCC TAAGGGGAAA TCACGAAACA CGCATGCTTT CCTCGGTTTA CGGCTTCAAG GAAGAGTGCA TGCGTAAGTA CGACCTTGTA GTGTACTGGA GGATTTGCGA GACCTTTCAG TATCTTCCGA TTGCCGCCGT GGTTCTGGAA AAGTACTTCT GTGTGCATGG CGGGATGGTG CCAGGGATGT CTCTGGACTT CATAATGAAA CATGACAGGA TAGGAGAGAT TACCGAGCTA AACGATGTCT TGTGGAGCGA TCCAGATGAG ACTCCCGGGT TTCAAAAAAG CCAAAGAGGT GCCGGGTATT TATTTGGAGA AGATGTCTTG AAAAGCTTTC TTGATGAACA TGGTCTATCT CATTTTGTTC GATCTCACCA GCTGGTTAAT GACGGCTACA AAGCATACTT TGACGAGAAG TTGTACACTG TGTGGGGAGC CCCGAACTAC TGCTATTCTT CTGGTAATAT TGCCTGTGTT ATGGCTATTG ACGAGGATGG TCATGATTTT AGACTGTTTG ATAGATGTGA AGAGCAGTTC AAAGAAGCTC CAGTAAGGCA TAGCTTCTTC GAC
Details for EncuA.00196.a.A1.GE27198
HARVESTED ON: 7/31/2009
SEQUENCED ON: 8/12/2009
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTKDHAEPK YLEILRRIKN SILPARGEVL ELVGEVRKIL EQEPNVLHID TPVNVVGDVH GHFYDVLNML DLMEDPSEKP YLFLGDYVDR GYNSVDLVLL LFVYKRLYRD RVHLLRGNHE TRMLSSVYGF KEECMRKYDL VVYWRICETF QYLPIAAVVL EKYFCVHGGM VPGMSLDFIM KHDRIGEITE LNDVLWSDPD ETPGFQKSQR GAGYLFGEDV SKSFLDEHGL SHFVRSHQLV NDGYKAYFDE KLYTVWGAPN YCYSSGNIAC VMAIDEDGHD FRLFDRCEEQ FKEAPVRHSF FD
Validated NT Sequence
ttattagtcg aagaagctat gccttactgg agcttctttg aactgctctt cacatctatc aaacagtcta aaatcatgac catcctcgtc aatagccata acacaggcaa tattaccaga agaatagcag tagttcgggg ctccccacac agtgtacaac ttctcgtcaa agtatgcttt gtagccgtca ttaaccagct ggtgagatcg aacaaaatga gatagaccat gttcatcaag aaagcttttc gagacatctt ctccaaataa atacccggca cctctttggc ttttttgaaa cccgggagtc tcatctggat cgctccacaa gacatcgttt agctcggtaa tctctcctat cctgtcatgt ttcattatga agtccagaga catccctggc accatcccgc catgcacaca gaagtacttt tccagaacca cggcggcaat cggaagatac tgaaaggtct cgcaaatcct ccagtacact acaaggtcgt acttacgcat gcactcttcc ttgaagccgt aaaccgagga aagcatgcgt gtttcgtgat ttccccttag gagatgcacc ctgtctctat agagtctctt gtatacaaac aggagaagaa caaggtcaac agagttgtat cccctgtcga catagtctcc aaggaatagg tatggctttt ctgacggatc ctccatcagg tccagcatgt tcagtacatc atagaagtgg ccatgcacat ctccgacaac gttaaccggc gtatctatgt gcagaacatt aggttcctgt tcgagtatct tcctgacctc ccctaccaac tccagaacct cgcctcttgc gggaagaatg gagttcttga tccttcgaag aatttccaga tactttggct cagcgtggtc ctttgtcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc atggtatatc tcctt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKDHAEPK YLEILRRIKN SILPARDEVL ELVGEVRKIL EQEPNVLHID TPVNVVGDVH GHFYDVLNML DLMEDPSEKP YLFLGDYVDR GYNSVDLVLL LFVYKRLYRD RVHLLRGNHE TRMLSSVYGF KEECMRKYDL VVYWRICETF QYLPIAAVVL EKYFCVHGGM VPGMSLDFIM KHDRIGEITE LNDVLWSDPD ETPGFQKSQR GAGYLFGEDV LKSFLDEHGL SHFVRSHQLV NDGYKAYFDE KLYTVWGAPN YCYSSGNIAC VMAIDEDGHD FRLFDRCEEQ FKEAPVRHSF FD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaaaggac cacgctgagc caaagtatct ggaaattctt cgaaggatca agaactccat tcttcctgca agagacgagg ttctggagtt ggtaggggag gtcaggaaga tactcgaaca ggaacctaat gttctgcaca tagatacgcc ggttaacgtt gtcggagatg tgcatggcca cttctatgat gtactgaaca tgctggacct gatggaggat ccgtcagaaa agccatacct attccttgga gactatgtcg acaggggata caactctgtt gaccttgttc ttctcctgtt tgtatacaag agactctata gagacagggt gcatctccta aggggaaatc acgaaacacg catgctttcc tcggtttacg gcttcaagga agagtgcatg cgtaagtacg accttgtagt gtactggagg atttgcgaga cctttcagta tcttccgatt gccgccgtgg ttctggaaaa gtacttctgt gtgcatggcg ggatggtgcc agggatgtct ctggacttca taatgaaaca tgacaggata ggagagatta ccgagctaaa cgatgtcttg tggagcgatc cagatgagac tcccgggttt caaaaaagcc aaagaggtgc cgggtattta tttggagaag atgtcttgaa aagctttctt gatgaacatg gtctatctca ttttgttcga tctcaccagc tggttaatga cggctacaaa gcatactttg acgagaagtt gtacactgtg tggggagccc cgaactactg ctattcttct ggtaatattg cctgtgttat ggctattgac gaggatggtc atgattttag actgtttgat agatgtgaag agcagttcaa agaagctcca gtaaggcata gcttcttcga caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa