EncuA.00177.a

Heat shock protein homolog SSE1

CENTER ID: EncuA.00177.a
ORGANISM: Encephalitozoon cuniculi GB-M1
ASSOCIATED DISEASE: Microsporidiosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/IIIB

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EncuA.00177.a.A1.PW30748 Full length( EncuA.00177.a ) 1 664

External Resources

RESOURCE REFERENCE ID
EuPathDB: MicrosporidiaDB:ECU11_1830
RefSeq: NP_586489.1
UniProt: Q8SQR8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVTLALMEFV GIDIGNYKTV IASSKENGKV YGDEQGKRSI RTVMELSTPV RRFGNGVTND VEQSLDLRSR SFRDALEDKK GWGNLAMFMK YIDRVVKKNT PTHPPICMAV PAYFKERERR ILVDIANTMD FKLEGLITDI SAIAMFACVR RENMPSEFLL FDFGFSKTTA GLFSFEKNVL KPLYMKTVRV GSMQFDEKLI DIIVEKHSLE KSRLVREKIK RNLDKIKTTL NSTKCCNIQL FITENPLEVI ITQEEYRNAV KSYLSDLDSF VSSVIKETEF NGLVEVVGGN SISFLIKEML RDKVEYQVTL DVSDSTAIGA ALGMACMSLR TRYSLHDIVG REISIRIQGE DVSPTVIFKS TELVEGNPKI VTYNRKESFV LEILEDGEVI STLNVVKGET KETKAIHVSF SIGKFGTVCV NSVECEESVD YEYKPFRISD IDLDDIKALE MKYRDGELGL ERIGTMRNEL ETMAVGLGDA LYNKFGKITN DEELNTVREV AMDLFDMPQS ETVGQEEEVR NTILSKLEFI SKKLSDYRDA AVEDLKKHKD MINEFRKEYG SVFTPSFYKL QGLLYKVDEY LKDFDLNLFN VKLFDESFIM EIKGDIQKYL EKAKLEIEEK RKEEERKSKK ENAQEGTSSK PESKEESEAK EDNDEESDVA SIDE
NT Sequence
ATGGTGACTC TGGCCCTAAT GGAGTTTGTC GGAATTGATA TCGGCAACTA TAAAACAGTG ATTGCCTCTT CCAAGGAGAA TGGGAAGGTA TATGGCGACG AGCAAGGGAA AAGGTCTATA AGGACTGTAA TGGAGCTTAG TACGCCAGTG CGCAGGTTTG GAAATGGGGT GACCAACGAC GTTGAGCAAT CACTTGACTT GAGGAGTAGG AGCTTCCGGG ATGCCCTAGA GGACAAGAAG GGATGGGGGA ACCTTGCGAT GTTCATGAAG TATATTGATA GGGTTGTGAA GAAGAACACT CCCACCCATC CTCCAATATG CATGGCCGTT CCTGCTTACT TCAAAGAGAG AGAAAGAAGA ATCTTGGTGG ACATTGCAAA TACGATGGAC TTTAAGCTTG AGGGGCTTAT TACGGACATA TCTGCAATAG CGATGTTTGC ATGCGTTAGA AGAGAAAATA TGCCAAGTGA GTTCCTTCTG TTTGATTTCG GGTTTTCAAA GACTACTGCT GGGCTTTTTA GCTTTGAAAA GAATGTCCTT AAGCCGCTGT ATATGAAGAC TGTGAGGGTG GGATCTATGC AATTTGATGA AAAGCTGATT GACATCATTG TTGAGAAGCA CTCTCTAGAG AAAAGCCGCC TCGTCCGAGA AAAGATCAAG AGGAATCTTG ACAAGATTAA GACAACATTG AACTCCACGA AGTGTTGCAA TATCCAACTG TTCATTACGG AGAATCCTCT GGAGGTGATT ATCACGCAGG AGGAGTATAG GAATGCAGTT AAGAGCTATC TAAGCGATTT GGACTCGTTT GTTAGTTCGG TTATAAAGGA AACTGAATTC AATGGGCTGG TGGAGGTTGT TGGAGGGAAC TCGATTTCGT TCCTTATTAA GGAGATGCTG AGGGATAAGG TTGAATATCA GGTAACGTTG GATGTTTCAG ATTCTACTGC AATTGGTGCG GCACTTGGGA TGGCATGCAT GTCTTTAAGG ACCAGATATT CGCTTCATGA TATTGTGGGA AGGGAGATTT CGATCCGAAT CCAGGGGGAG GATGTCAGCC CAACGGTGAT ATTCAAGTCG ACGGAGCTGG TTGAGGGAAA TCCAAAGATA GTTACATACA ACAGAAAGGA AAGCTTTGTG TTGGAGATTC TGGAGGACGG AGAAGTGATT TCGACTCTAA ACGTTGTGAA AGGTGAGACA AAGGAGACAA AGGCGATCCA TGTGTCTTTT TCGATAGGAA AGTTTGGAAC GGTTTGTGTG AACTCTGTGG AATGTGAAGA GTCTGTCGAC TATGAATACA AGCCCTTCAG GATATCTGAT ATTGATCTGG ATGATATCAA GGCATTGGAG ATGAAGTACA GAGACGGAGA GCTGGGGCTC GAAAGAATAG GAACGATGAG GAATGAGCTA GAGACTATGG CTGTAGGGCT GGGAGATGCT CTGTATAACA AGTTCGGTAA GATTACAAAC GACGAAGAAT TGAATACAGT TAGGGAAGTA GCTATGGATT TGTTTGATAT GCCACAGTCT GAAACTGTAG GACAAGAAGA GGAGGTCCGA AATACTATTC TGTCGAAGTT GGAGTTCATA TCCAAGAAGC TTAGCGATTA TAGGGACGCT GCTGTTGAAG ACCTTAAGAA GCACAAGGAT ATGATCAACG AGTTTAGGAA GGAGTACGGT AGTGTTTTTA CGCCTAGCTT CTACAAGCTG CAAGGCCTTC TCTACAAAGT GGATGAGTAT CTTAAGGACT TCGACCTGAA TCTATTCAAT GTGAAATTGT TTGATGAAAG CTTTATAATG GAGATTAAGG GCGACATTCA GAAGTATCTA GAAAAAGCCA AGCTCGAGAT TGAAGAGAAA AGAAAAGAGG AAGAAAGGAA AAGTAAGAAG GAGAATGCTC AGGAAGGCAC TAGCAGCAAG CCTGAGTCTA AAGAGGAATC AGAAGCCAAG GAAGACAATG ATGAGGAATC TGACGTTGCA AGCATTGACG AA
Details for EncuA.00177.a.A1.PW30748
PURIFICATION DATe: 2/5/2011
CONCENTRATION: 35.45mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 51
Protocol Notes
notes unavailable
Validated AA Sequence
KSTXLVEXNP KIVTYNRKES FVLEILEDGE VISTLNVVKG ETKETKAIHV SFSIGKFGTV CVNSVECEES VDYEYKPFRI SDIDLDDIKA LEMKYRDGEL GLERIGTMRN ELETMAVGLG DALYNKFGKI TNDEELNTVR EVAMDLFDMP QSETVGQEEE VRNTILSKLE FISKKLSDYR DAAVEDLKKH KDMINEFRKE YGSVFTPSFY KLQGLLYKVD EYLKDFDLNL FNVKLFDESF IMEIKGDIQK YLEKAKLEIE EKRKEEERKS KKENAQEGTS SKPESKEESE AKEDNDEESD VASIDE
Validated NT Sequence
nnnnnnnnnn nnncntnnnn nnnnntgtta gcagccggat cctcgagaag cttggctgca gaacttgttc gtgctgttta ttattcgtca atgcttgcaa cgtcagattc ctcatcattg tcttccttgg cttctgattc ctctttagac tcaggcttgc tgctagtgcc ttcctgagca ttctccttct tacttttcct ttcttcctct tttcttttct cttcaatctc gagcttggct ttttctagat acttctgaat gtcgccctta atctccatta taaagctttc atcaaacaat ttcacattga atagattcag gtcgaagtcc ttaagatact catccacttt gtagagaagg ccttgcagct tgtagaagct aggcgtaaaa acactaccgt actccttcct aaactcgttg atcatatcct tgtgcttctt aaggtcttca acagcagcgt ccctataatc gctaagcttc ttggatatga actccaactt cgacagaata gtatttcgga cctcctcttc ttgtcctaca gtttcagact gtggcatatc aaacaaatcc atagctactt ccctaactgt attcaattct tcgtcgtttg taatcttacc gaacttgtta tacagagcat ctcccagccc tacagccata gtctctagct cattcctcat cgttcctatt ctttcgagcc ccagctctcc gtctctgtac ttcatctcca atgccttgat atcatccaga tcaatatcag atatcctgaa gggcttgtat tcatagtcga cagactcttc acattccaca gagttcacac aaaccgttcc aaactttcct atcgaaaaag acacatggat cgcctttgtc tcctttgtct cacctttcac aacgtttaga gtcgaaatca cttctccgtc ctccagaatc tccaacacaa agctttcctt tctgttgtat gtaactatct ttggatttnc ctcaaccagc tncgtcgact tgannatcan cgnngggntg annncncccc cnngnnnnnn atcnaantct nccnnnccnn nnatcatnnn nnananctng nnnnnnannn anatgcannn nnccnnnnnn nnnnnnnnng nnnnnnnnnn tnnaanntcn annnnannnn nnnnnnnnnn nnnnnnncnn tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnn
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTLALMEF VGIDIGNYKT VIASSKENGK VYGDEQGKRS IRTVMELSTP VRRFGNGVTN DVEQSLDLRS RSFRDALEDK KGWGNLAMFM KYIDRVVKKN TPTHPPICMA VPAYFKERER RILVDIANTM DFKLEGLITD ISAIAMFACV RRENMPSEFL LFDFGFSKTT AGLFSFEKNV LKPLYMKTVR VGSMQFDEKL IDIIVEKHSL EKSRLVREKI KRNLDKIKTT LNSTKCCNIQ LFITENPLEV IITQEEYRNA VKSYLSDLDS FVSSVIKETE FNGLVEVVGG NSISFLIKEM LRDKVEYQVT LDVSDSTAIG AALGMACMSL RTRYSLHDIV GREISIRIQG EDVSPTVIFK STELVEGNPK IVTYNRKESF VLEILEDGEV ISTLNVVKGE TKETKAIHVS FSIGKFGTVC VNSVECEESV DYEYKPFRIS DIDLDDIKAL EMKYRDGELG LERIGTMRNE LETMAVGLGD ALYNKFGKIT NDEELNTVRE VAMDLFDMPQ SETVGQEEEV RNTILSKLEF ISKKLSDYRD AAVEDLKKHK DMINEFRKEY GSVFTPSFYK LQGLLYKVDE YLKDFDLNLF NVKLFDESFI MEIKGDIQKY LEKAKLEIEE KRKEEERKSK KENAQEGTSS KPESKEESEA KEDNDEESDV ASIDE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACTCTG GCCCTAATGG AGTTTGTCGG AATTGATATC GGCAACTATA AAACAGTGAT TGCCTCTTCC AAGGAGAATG GGAAGGTATA TGGCGACGAG CAAGGGAAAA GGTCTATAAG GACTGTAATG GAGCTTAGTA CGCCAGTGCG CAGGTTTGGA AATGGGGTGA CCAACGACGT TGAGCAATCA CTTGACTTGA GGAGTAGGAG CTTCCGGGAT GCCCTAGAGG ACAAGAAGGG ATGGGGGAAC CTTGCGATGT TCATGAAGTA TATTGATAGG GTTGTGAAGA AGAACACTCC CACCCATCCT CCAATATGCA TGGCCGTTCC TGCTTACTTC AAAGAGAGAG AAAGAAGAAT CTTGGTGGAC ATTGCAAATA CGATGGACTT TAAGCTTGAG GGGCTTATTA CGGACATATC TGCAATAGCG ATGTTTGCAT GCGTTAGAAG AGAAAATATG CCAAGTGAGT TCCTTCTGTT TGATTTCGGG TTTTCAAAGA CTACTGCTGG GCTTTTTAGC TTTGAAAAGA ATGTCCTTAA GCCGCTGTAT ATGAAGACTG TGAGGGTGGG ATCTATGCAA TTTGATGAAA AGCTGATTGA CATCATTGTT GAGAAGCACT CTCTAGAGAA AAGCCGCCTC GTCCGAGAAA AGATCAAGAG GAATCTTGAC AAGATTAAGA CAACATTGAA CTCCACGAAG TGTTGCAATA TCCAACTGTT CATTACGGAG AATCCTCTGG AGGTGATTAT CACGCAGGAG GAGTATAGGA ATGCAGTTAA GAGCTATCTA AGCGATTTGG ACTCGTTTGT TAGTTCGGTT ATAAAGGAAA CTGAATTCAA TGGGCTGGTG GAGGTTGTTG GAGGGAACTC GATTTCGTTC CTTATTAAGG AGATGCTGAG GGATAAGGTT GAATATCAGG TAACGTTGGA TGTTTCAGAT TCTACTGCAA TTGGTGCGGC ACTTGGGATG GCATGCATGT CTTTAAGGAC CAGATATTCG CTTCATGATA TTGTGGGAAG GGAGATTTCG ATCCGAATCC AGGGGGAGGA TGTCAGCCCA ACGGTGATAT TCAAGTCGAC GGAGCTGGTT GAGGGAAATC CAAAGATAGT TACATACAAC AGAAAGGAAA GCTTTGTGTT GGAGATTCTG GAGGACGGAG AAGTGATTTC GACTCTAAAC GTTGTGAAAG GTGAGACAAA GGAGACAAAG GCGATCCATG TGTCTTTTTC GATAGGAAAG TTTGGAACGG TTTGTGTGAA CTCTGTGGAA TGTGAAGAGT CTGTCGACTA TGAATACAAG CCCTTCAGGA TATCTGATAT TGATCTGGAT GATATCAAGG CATTGGAGAT GAAGTACAGA GACGGAGAGC TGGGGCTCGA AAGAATAGGA ACGATGAGGA ATGAGCTAGA GACTATGGCT GTAGGGCTGG GAGATGCTCT GTATAACAAG TTCGGTAAGA TTACAAACGA CGAAGAATTG AATACAGTTA GGGAAGTAGC TATGGATTTG TTTGATATGC CACAGTCTGA AACTGTAGGA CAAGAAGAGG AGGTCCGAAA TACTATTCTG TCGAAGTTGG AGTTCATATC CAAGAAGCTT AGCGATTATA GGGACGCTGC TGTTGAAGAC CTTAAGAAGC ACAAGGATAT GATCAACGAG TTTAGGAAGG AGTACGGTAG TGTTTTTACG CCTAGCTTCT ACAAGCTGCA AGGCCTTCTC TACAAAGTGG ATGAGTATCT TAAGGACTTC GACCTGAATC TATTCAATGT GAAATTGTTT GATGAAAGCT TTATAATGGA GATTAAGGGC GACATTCAGA AGTATCTAGA AAAAGCCAAG CTCGAGATTG AAGAGAAAAG AAAAGAGGAA GAAAGGAAAA GTAAGAAGGA GAATGCTCAG GAAGGCACTA GCAGCAAGCC TGAGTCTAAA GAGGAATCAG AAGCCAAGGA AGACAATGAT GAGGAATCTG ACGTTGCAAG CATTGACGAA TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA