SSGCID: Target Page

ElmeA.01238.a

Seryl-tRNA ligase

CENTER ID:	ElmeA.01238.a
ORGANISM:	Elizabethkingia meningoseptica ATCC 13253 (NBRC 12535)
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElmeA.01238.a.B1.GE42444	Full length(ElmeA.01238.a)		1	421

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
UniProt:	R9CQZ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLQVSFLRDE KARVLEGLQK RNFKHLELVD QAVSTDDQRK KIQFELDSQL SEMNKISKEI GALMKEGKKD EAEVAKQRTT DIKESIKELQ QELNETEKAL LNILYQIPNV PHELVKAGVS EEDNETVFQS CDVEGLGEGA IPHWELATKY NIINFELGVK VAGAGFPVYL GKGARLQRAL VQYFLDKNTN AGYIEVNPPH VVNEASGYGT GQLPDKEGQM YYINEDQLYL IPTAEVPVTN IYRDELLEEK QLPIMHTAFS QCYRREAGSY GAHVRGLNRL HQFEKVEIVR FEKPENSYAA LDGMVEHVKG ILNDLGLPYR ILRLCGGDTG FGAAMTYDFE VWSAAQEKWL EVSSVSNFET FQANRLKCRY KTDGKTQLVH TLNGSAMALP RIMAAILENN QTADGIAIPE VLRPYTGFDK I

NT Sequence

ATGTTACAAG TTAGTTTTTT GCGTGACGAA AAAGCGCGCG TTTTAGAAGG TTTACAAAAG AGAAATTTCA AACATCTTGA GTTGGTAGAT CAGGCTGTGT CCACAGATGA CCAACGTAAG AAAATTCAGT TTGAACTTGA CTCACAACTT TCCGAAATGA ATAAAATCTC GAAAGAGATT GGTGCTTTAA TGAAGGAAGG TAAAAAAGAT GAGGCAGAAG TTGCCAAGCA AAGAACAACA GATATTAAAG AAAGTATTAA AGAATTACAG CAGGAACTTA ACGAAACAGA AAAGGCTTTG TTGAATATTC TTTATCAGAT CCCTAATGTT CCGCATGAAC TTGTAAAGGC GGGTGTATCT GAAGAGGATA ATGAAACAGT ATTTCAGAGT TGCGATGTAG AAGGGCTTGG TGAAGGTGCT ATTCCACATT GGGAATTAGC AACGAAATAC AATATTATTA ACTTTGAGTT AGGTGTTAAG GTTGCCGGTG CCGGATTCCC TGTTTATTTG GGTAAAGGGG CGCGTTTACA GCGTGCATTA GTACAGTATT TCCTTGATAA GAATACAAAT GCCGGATATA TTGAAGTAAA CCCGCCCCAC GTGGTAAATG AAGCTTCAGG TTACGGAACA GGACAGTTAC CGGATAAAGA AGGCCAGATG TATTATATCA ACGAGGATCA GTTATACCTT ATTCCTACTG CAGAAGTTCC TGTAACAAAC ATCTACCGTG ATGAATTATT GGAAGAGAAG CAGCTGCCTA TTATGCATAC TGCATTTTCT CAGTGTTACA GAAGAGAAGC AGGTTCCTAC GGGGCACATG TAAGAGGGCT GAACAGATTA CATCAGTTTG AAAAAGTAGA AATCGTAAGA TTTGAAAAGC CGGAAAATTC TTATGCTGCG CTTGATGGAA TGGTGGAGCA TGTAAAGGGT ATTCTTAATG ATCTTGGACT TCCGTACCGT ATTTTAAGAT TATGCGGAGG AGATACAGGA TTTGGTGCTG CTATGACCTA TGACTTTGAG GTATGGAGTG CTGCACAGGA AAAATGGCTT GAAGTAAGTT CTGTATCTAA CTTCGAAACG TTTCAGGCAA ACCGTTTAAA GTGCCGTTAT AAGACTGATG GTAAAACACA GCTGGTACAT ACATTAAATG GCTCTGCAAT GGCTTTACCA AGAATTATGG CAGCTATCCT GGAGAACAAC CAGACAGCTG ACGGTATTGC TATTCCGGAA GTATTAAGAC CTTACACCGG TTTTGATAAG ATC

Details for ElmeA.01238.a.B1.GE42444

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	52
PERCENT COVERAGE:	12

Validated AA Sequence

MAHHHHHHML QVSFLRVFFT KLFRKVVLPV PALPVKKTLL FVCSSNSTSA SNE

Validated NT Sequence

ttgatggata aaggtttgcg ggtgttcgat tctttcgatt gattttattt ataaaaaata aattatggct ttaataaaag aattactggg gaaaacaccg gagtttggag agaatgttta tctggcagaa actgcaacga taattggtga tgttaaaatg ggaaaagact gcagcgtatg gtttaatgca gtgattagag gtgatgtaaa cagtataagg atcggtaatc aggtaaatat tcaggataat gcaatgattc actgtacttt cgaaaagact gaaactataa ttggtgacaa tgtttctatt ggccataatg caattgttca tggatgtcag gtacatgata atgttcttat tggaatggga gccattgtaa tggacgattg tgtcgtagaa aataattcga ttgttgctgc cggagccgtt gttactcaag ggacacacat taaagccgga gaaatctggg caggagtgcc ggcaaaaaaa ataaaatcgg tttctgtaga cttactggaa ggagaaattc acagaatatc gaataactac atcaaatact cttcgtggta taaagaagat taataattac tacttgttct atatgctttt ataatcggtg aaagcatata actgaaaatc taaaatacag aaaagcattt ccgtaaggat tgcttttcgc ttttttatgc cttaactttg atgaaacttt ttcattcatt ggaggcactc gtcgaattac ttgaacatac aaacagaagc gtctttttaa caggtaaagc aggaacaggt aagactacct ttctgaataa ttttgtaaag aatacacgca aaaaactaac ttgtaacata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHML QVSFLRDEKA RVLEGLQKRN FKHLELVDQA VSTDDQRKKI QFELDSQLSE MNKISKEIGA LMKEGKKDEA EVAKQRTTDI KESIKELQQE LNETEKALLN ILYQIPNVPH ELVKAGVSEE DNETVFQSCD VEGLGEGAIP HWELATKYNI INFELGVKVA GAGFPVYLGK GARLQRALVQ YFLDKNTNAG YIEVNPPHVV NEASGYGTGQ LPDKEGQMYY INEDQLYLIP TAEVPVTNIY RDELLEEKQL PIMHTAFSQC YRREAGSYGA HVRGLNRLHQ FEKVEIVRFE KPENSYAALD GMVEHVKGIL NDLGLPYRIL RLCGGDTGFG AAMTYDFEVW SAAQEKWLEV SSVSNFETFQ ANRLKCRYKT DGKTQLVHTL NGSAMALPRI MAAILENNQT ADGIAIPEVL RPYTGFDKI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttac aagttagttt tttgcgtgac gaaaaagcgc gcgttttaga aggtttacaa aagagaaatt tcaaacatct tgagttggta gatcaggctg tgtccacaga tgaccaacgt aagaaaattc agtttgaact tgactcacaa ctttccgaaa tgaataaaat ctcgaaagag attggtgctt taatgaagga aggtaaaaaa gatgaggcag aagttgccaa gcaaagaaca acagatatta aagaaagtat taaagaatta cagcaggaac ttaacgaaac agaaaaggct ttgttgaata ttctttatca gatccctaat gttccgcatg aacttgtaaa ggcgggtgta tctgaagagg ataatgaaac agtatttcag agttgcgatg tagaagggct tggtgaaggt gctattccac attgggaatt agcaacgaaa tacaatatta ttaactttga gttaggtgtt aaggttgccg gtgccggatt ccctgtttat ttgggtaaag gggcgcgttt acagcgtgca ttagtacagt atttccttga taagaataca aatgccggat atattgaagt aaacccgccc cacgtggtaa atgaagcttc aggttacgga acaggacagt taccggataa agaaggccag atgtattata tcaacgagga tcagttatac cttattccta ctgcagaagt tcctgtaaca aacatctacc gtgatgaatt attggaagag aagcagctgc ctattatgca tactgcattt tctcagtgtt acagaagaga agcaggttcc tacggggcac atgtaagagg gctgaacaga ttacatcagt ttgaaaaagt agaaatcgta agatttgaaa agccggaaaa ttcttatgct gcgcttgatg gaatggtgga gcatgtaaag ggtattctta atgatcttgg acttccgtac cgtattttaa gattatgcgg aggagataca ggatttggtg ctgctatgac ctatgacttt gaggtatgga gtgctgcaca ggaaaaatgg cttgaagtaa gttctgtatc taacttcgaa acgtttcagg caaaccgttt aaagtgccgt tataagactg atggtaaaac acagctggta catacattaa atggctctgc aatggcttta ccaagaatta tggcagctat cctggagaac aaccagacag ctgacggtat tgctattccg gaagtattaa gaccttacac cggttttgat aagatctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t