ElmeA.00612.a

Lysine--tRNA ligase

CENTER ID: ElmeA.00612.a
ORGANISM: Elizabethkingia meningoseptica ATCC 13253 (NBRC 12535)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElmeA.00612.a.B1.GE42442 Full length(ElmeA.00612.a) 1 566
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElmeA.00612.a.B1.PW38527 Full length(ElmeA.00612.a) 1 566

External Resources

RESOURCE REFERENCE ID
UniProt: R9CGY9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQLTEQEIIR REKLQQLRQM GIEPYPAAEF EVNTNTQEIK QKYEDGKKVK LAGRLMSVRI MGKASFAELQ DSEGRVQIYV SRDDISSSEE AVEYNTVFKK LLDIGDFIGI EGHLFKTQVG EISVHVTKLA LLSKTLRPLP VVKTDEDGKV HDAFVDPELR YRMRYVDLAV NPHVKDIFIK RTKLFNAMRG FFNDKGYFEV ETPVLQSIPG GAAARPFITH HNALDIPLYL RIANELYLKR LIVGGFDGVY EFSKNFRNEG MDRTHNPEFT AMEIYVAYKD YNWMMDFTEK LLEHCAIAVN GTSKANFGEY EIDFKAPYPR VSMTEAIQKY TGYDITGKTE EELRAFAKSI GLDVDETMGK GKLIDEIFGE KCEGNFIQPT FITDYPVEMS PLTKKHRSKE GLTERFELMV CGKEIANAYS ELNDPIDQRA RFEDQLKLSE KGDDEAGQFI DEDFLRALEY GMPPTSGLGI GMDRLIMFLT NNPSIQEVLF FPQMRPEKTE PKIELNEDEK AIIEIVGAQE EPMQLSEVKD KSQLSGKKWD KSIKNLTKNN LVKVEKVDEV LLIALV
NT Sequence
ATGCAACTTA CAGAGCAAGA AATCATCCGC AGGGAAAAAC TGCAACAACT GAGACAGATG GGAATCGAGC CTTATCCGGC AGCAGAATTT GAAGTGAATA CAAATACGCA GGAGATAAAG CAGAAGTACG AGGACGGGAA GAAGGTGAAG TTGGCAGGGC GCTTAATGAG TGTGCGTATT ATGGGGAAAG CTTCATTTGC TGAACTTCAG GATAGCGAAG GACGTGTACA GATTTATGTA TCACGCGATG ATATTTCCAG TTCTGAGGAA GCTGTAGAAT ATAACACTGT ATTCAAAAAA CTTTTAGATA TTGGTGACTT TATCGGGATT GAAGGACACC TGTTTAAAAC ACAGGTAGGA GAGATCTCTG TTCATGTAAC CAAACTTGCG CTTCTTTCTA AAACATTACG CCCGTTACCG GTAGTTAAAA CGGATGAGGA CGGAAAAGTA CACGATGCTT TTGTTGATCC GGAATTGCGT TACCGTATGC GTTATGTAGA CTTAGCAGTT AATCCGCATG TTAAAGATAT CTTCATTAAA AGAACAAAAT TATTCAACGC TATGCGTGGT TTCTTTAATG ATAAAGGTTA CTTCGAAGTA GAAACACCGG TATTACAATC TATTCCCGGT GGCGCTGCAG CAAGACCTTT CATTACACAC CACAATGCAT TGGATATTCC ATTATACCTT CGTATTGCAA ACGAATTATA TCTGAAAAGA TTGATCGTAG GTGGTTTTGA TGGTGTTTAT GAGTTTTCCA AGAACTTCCG TAATGAAGGG ATGGACAGAA CACACAATCC GGAATTTACC GCAATGGAAA TTTATGTAGC TTATAAAGAC TACAACTGGA TGATGGACTT TACTGAAAAG TTATTGGAAC ATTGTGCTAT TGCTGTTAAC GGAACTTCTA AAGCGAACTT CGGAGAATAC GAAATTGATT TTAAAGCACC ATACCCAAGA GTTTCTATGA CGGAAGCTAT TCAGAAATAT ACGGGCTATG ATATTACCGG GAAAACTGAA GAAGAACTTC GTGCTTTTGC GAAGTCAATT GGTCTTGATG TGGATGAAAC AATGGGTAAA GGAAAACTTA TTGATGAAAT CTTCGGAGAA AAATGTGAAG GAAACTTTAT TCAGCCAACG TTTATTACCG ATTATCCAGT AGAAATGTCT CCGCTAACTA AAAAACACAG AAGCAAAGAA GGTTTAACAG AGCGTTTCGA GCTTATGGTT TGTGGTAAGG AGATTGCAAA TGCTTATTCA GAGCTTAATG ACCCGATTGA TCAGAGAGCG CGTTTTGAAG ATCAGCTAAA GCTTTCTGAA AAAGGAGATG ATGAAGCAGG ACAATTTATT GATGAAGATT TCCTTAGAGC GTTGGAATAC GGAATGCCAC CAACATCCGG TTTAGGAATC GGTATGGACA GATTGATTAT GTTCTTAACG AACAACCCAT CTATTCAGGA AGTATTATTC TTCCCGCAGA TGCGTCCGGA GAAAACTGAA CCTAAGATTG AGCTTAATGA AGATGAGAAG GCAATTATTG AAATTGTAGG CGCTCAGGAA GAACCAATGC AGCTTTCGGA AGTGAAAGAT AAAAGCCAGT TATCCGGTAA GAAATGGGAT AAGTCTATAA AGAATCTTAC TAAAAATAAT CTGGTAAAAG TTGAGAAAGT AGATGAGGTT TTACTAATTG CATTAGTA
Details for ElmeA.00612.a.B1.GE42442
HARVESTED ON: 5/25/2018
SEQUENCED ON: 5/31/2018
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 95
PERCENT COVERAGE: 51
Validated AA Sequence
MXRXXXPEXX AMEIYVXXKD YXWMMXXTEK LLXHCAIAVN GTSKANFGEY EIDFKAPYPR VSMTEAIQKY TGYDITGKTE EELRAFAKSI GLDVDETMGK GKLIDEIFGE KCEGNFIQPT FITDYPVEMS PLTKKHRSKE GLTERFELMV CGKEIANAYS ELNDPIDQRA RFEDQLKLSE KGDDEAGQFI DEDFLRALEY GMPPTSGLGI GMDRLIMFLT NNPSIQEVLF FPQMRPEKTE PKIELNEDEK AIIEIVGAQE EPMQLSEVKD KSQLSGKKWD KSIKNLTKNN LVKVEKVD
Validated NT Sequence
tcatctactt tctcaacttt taccagatta tttttagtaa gattctttat agacttatcc catttcttac cggataactg gcttttatct ttcacttccg aaagctgcat tggttcttcc tgagcgccta caatttcaat aattgccttc tcatcttcat taagctcaat cttaggttca gttttctccg gacgcatctg cgggaagaat aatacttcct gaatagatgg gttgttcgtt aagaacataa tcaatctgtc cataccgatt cctaaaccgg atgttggtgg cattccgtat tccaacgctc taaggaaatc ttcatcaata aattgtcctg cttcatcatc tcctttttca gaaagcttta gctgatcttc aaaacgcgct ctctgatcaa tcgggtcatt aagctctgaa taagcatttg caatctcctt accacaaacc ataagctcga aacgctctgt taaaccttct ttgcttctgt gttttttagt tagcggagac atttctactg gataatcggt aataaacgtt ggctgaataa agtttccttc acatttttct ccgaagattt catcaataag ttttccttta cccattgttt catccacatc aagaccaatt gacttcgcaa aagcacgaag ttcttcttca gttttcccgg taatatcata gcccgtatat ttctgaatag cttccgtcat agaaactctt gggtatggtg ctttaaaatc aatttcgtat tctccgaagt tcgctttaga agttccgtta acagcaatag cacaatgntc caataacttt tcagtaannn ncatcatcca gntgtagtct ttannnncta cataaatttc cattgcnnnn aattccggan nnnnnnttct gtncatccct tcatnannnn nnnnnttgnn aaactcataa acaccatcaa aaccacctan nntcaatctt tncnnntata a
Expected Protein Sequence
MAHHHHHHMQ LTEQEIIRRE KLQQLRQMGI EPYPAAEFEV NTNTQEIKQK YEDGKKVKLA GRLMSVRIMG KASFAELQDS EGRVQIYVSR DDISSSEEAV EYNTVFKKLL DIGDFIGIEG HLFKTQVGEI SVHVTKLALL SKTLRPLPVV KTDEDGKVHD AFVDPELRYR MRYVDLAVNP HVKDIFIKRT KLFNAMRGFF NDKGYFEVET PVLQSIPGGA AARPFITHHN ALDIPLYLRI ANELYLKRLI VGGFDGVYEF SKNFRNEGMD RTHNPEFTAM EIYVAYKDYN WMMDFTEKLL EHCAIAVNGT SKANFGEYEI DFKAPYPRVS MTEAIQKYTG YDITGKTEEE LRAFAKSIGL DVDETMGKGK LIDEIFGEKC EGNFIQPTFI TDYPVEMSPL TKKHRSKEGL TERFELMVCG KEIANAYSEL NDPIDQRARF EDQLKLSEKG DDEAGQFIDE DFLRALEYGM PPTSGLGIGM DRLIMFLTNN PSIQEVLFFP QMRPEKTEPK IELNEDEKAI IEIVGAQEEP MQLSEVKDKS QLSGKKWDKS IKNLTKNNLV KVEKVDEVLL IALV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac ttacagagca agaaatcatc cgcagggaaa aactgcaaca actgagacag atgggaatcg agccttatcc ggcagcagaa tttgaagtga atacaaatac gcaggagata aagcagaagt acgaggacgg gaagaaggtg aagttggcag ggcgcttaat gagtgtgcgt attatgggga aagcttcatt tgctgaactt caggatagcg aaggacgtgt acagatttat gtatcacgcg atgatatttc cagttctgag gaagctgtag aatataacac tgtattcaaa aaacttttag atattggtga ctttatcggg attgaaggac acctgtttaa aacacaggta ggagagatct ctgttcatgt aaccaaactt gcgcttcttt ctaaaacatt acgcccgtta ccggtagtta aaacggatga ggacggaaaa gtacacgatg cttttgttga tccggaattg cgttaccgta tgcgttatgt agacttagca gttaatccgc atgttaaaga tatcttcatt aaaagaacaa aattattcaa cgctatgcgt ggtttcttta atgataaagg ttacttcgaa gtagaaacac cggtattaca atctattccc ggtggcgctg cagcaagacc tttcattaca caccacaatg cattggatat tccattatac cttcgtattg caaacgaatt atatctgaaa agattgatcg taggtggttt tgatggtgtt tatgagtttt ccaagaactt ccgtaatgaa gggatggaca gaacacacaa tccggaattt accgcaatgg aaatttatgt agcttataaa gactacaact ggatgatgga ctttactgaa aagttattgg aacattgtgc tattgctgtt aacggaactt ctaaagcgaa cttcggagaa tacgaaattg attttaaagc accataccca agagtttcta tgacggaagc tattcagaaa tatacgggct atgatattac cgggaaaact gaagaagaac ttcgtgcttt tgcgaagtca attggtcttg atgtggatga aacaatgggt aaaggaaaac ttattgatga aatcttcgga gaaaaatgtg aaggaaactt tattcagcca acgtttatta ccgattatcc agtagaaatg tctccgctaa ctaaaaaaca cagaagcaaa gaaggtttaa cagagcgttt cgagcttatg gtttgtggta aggagattgc aaatgcttat tcagagctta atgacccgat tgatcagaga gcgcgttttg aagatcagct aaagctttct gaaaaaggag atgatgaagc aggacaattt attgatgaag atttccttag agcgttggaa tacggaatgc caccaacatc cggtttagga atcggtatgg acagattgat tatgttctta acgaacaacc catctattca ggaagtatta ttcttcccgc agatgcgtcc ggagaaaact gaacctaaga ttgagcttaa tgaagatgag aaggcaatta ttgaaattgt aggcgctcag gaagaaccaa tgcagctttc ggaagtgaaa gataaaagcc agttatccgg taagaaatgg gataagtcta taaagaatct tactaaaaat aatctggtaa aagttgagaa agtagatgag gttttactaa ttgcattagt atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for ElmeA.00612.a.B1.PW38527
PURIFICATION DATe: 9/20/2018
CONCENTRATION: 12.95mg/ml
OBSERVED MW: 66kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 32
VIAL VOLUME: 200µl
PERCENT IDENTITY: 95
PERCENT COVERAGE: 51
Protocol Notes
notes unavailable
Validated AA Sequence
MXRXXXPEXX AMEIYVXXKD YXWMMXXTEK LLXHCAIAVN GTSKANFGEY EIDFKAPYPR VSMTEAIQKY TGYDITGKTE EELRAFAKSI GLDVDETMGK GKLIDEIFGE KCEGNFIQPT FITDYPVEMS PLTKKHRSKE GLTERFELMV CGKEIANAYS ELNDPIDQRA RFEDQLKLSE KGDDEAGQFI DEDFLRALEY GMPPTSGLGI GMDRLIMFLT NNPSIQEVLF FPQMRPEKTE PKIELNEDEK AIIEIVGAQE EPMQLSEVKD KSQLSGKKWD KSIKNLTKNN LVKVEKVD
Validated NT Sequence
tcatctactt tctcaacttt taccagatta tttttagtaa gattctttat agacttatcc catttcttac cggataactg gcttttatct ttcacttccg aaagctgcat tggttcttcc tgagcgccta caatttcaat aattgccttc tcatcttcat taagctcaat cttaggttca gttttctccg gacgcatctg cgggaagaat aatacttcct gaatagatgg gttgttcgtt aagaacataa tcaatctgtc cataccgatt cctaaaccgg atgttggtgg cattccgtat tccaacgctc taaggaaatc ttcatcaata aattgtcctg cttcatcatc tcctttttca gaaagcttta gctgatcttc aaaacgcgct ctctgatcaa tcgggtcatt aagctctgaa taagcatttg caatctcctt accacaaacc ataagctcga aacgctctgt taaaccttct ttgcttctgt gttttttagt tagcggagac atttctactg gataatcggt aataaacgtt ggctgaataa agtttccttc acatttttct ccgaagattt catcaataag ttttccttta cccattgttt catccacatc aagaccaatt gacttcgcaa aagcacgaag ttcttcttca gttttcccgg taatatcata gcccgtatat ttctgaatag cttccgtcat agaaactctt gggtatggtg ctttaaaatc aatttcgtat tctccgaagt tcgctttaga agttccgtta acagcaatag cacaatgntc caataacttt tcagtaannn ncatcatcca gntgtagtct ttannnncta cataaatttc cattgcnnnn aattccggan nnnnnnttct gtncatccct tcatnannnn nnnnnttgnn aaactcataa acaccatcaa aaccacctan nntcaatctt tncnnntata a
Expressed Protein Sequence
MAHHHHHHMQ LTEQEIIRRE KLQQLRQMGI EPYPAAEFEV NTNTQEIKQK YEDGKKVKLA GRLMSVRIMG KASFAELQDS EGRVQIYVSR DDISSSEEAV EYNTVFKKLL DIGDFIGIEG HLFKTQVGEI SVHVTKLALL SKTLRPLPVV KTDEDGKVHD AFVDPELRYR MRYVDLAVNP HVKDIFIKRT KLFNAMRGFF NDKGYFEVET PVLQSIPGGA AARPFITHHN ALDIPLYLRI ANELYLKRLI VGGFDGVYEF SKNFRNEGMD RTHNPEFTAM EIYVAYKDYN WMMDFTEKLL EHCAIAVNGT SKANFGEYEI DFKAPYPRVS MTEAIQKYTG YDITGKTEEE LRAFAKSIGL DVDETMGKGK LIDEIFGEKC EGNFIQPTFI TDYPVEMSPL TKKHRSKEGL TERFELMVCG KEIANAYSEL NDPIDQRARF EDQLKLSEKG DDEAGQFIDE DFLRALEYGM PPTSGLGIGM DRLIMFLTNN PSIQEVLFFP QMRPEKTEPK IELNEDEKAI IEIVGAQEEP MQLSEVKDKS QLSGKKWDKS IKNLTKNNLV KVEKVDEVLL IALV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAC TTACAGAGCA AGAAATCATC CGCAGGGAAA AACTGCAACA ACTGAGACAG ATGGGAATCG AGCCTTATCC GGCAGCAGAA TTTGAAGTGA ATACAAATAC GCAGGAGATA AAGCAGAAGT ACGAGGACGG GAAGAAGGTG AAGTTGGCAG GGCGCTTAAT GAGTGTGCGT ATTATGGGGA AAGCTTCATT TGCTGAACTT CAGGATAGCG AAGGACGTGT ACAGATTTAT GTATCACGCG ATGATATTTC CAGTTCTGAG GAAGCTGTAG AATATAACAC TGTATTCAAA AAACTTTTAG ATATTGGTGA CTTTATCGGG ATTGAAGGAC ACCTGTTTAA AACACAGGTA GGAGAGATCT CTGTTCATGT AACCAAACTT GCGCTTCTTT CTAAAACATT ACGCCCGTTA CCGGTAGTTA AAACGGATGA GGACGGAAAA GTACACGATG CTTTTGTTGA TCCGGAATTG CGTTACCGTA TGCGTTATGT AGACTTAGCA GTTAATCCGC ATGTTAAAGA TATCTTCATT AAAAGAACAA AATTATTCAA CGCTATGCGT GGTTTCTTTA ATGATAAAGG TTACTTCGAA GTAGAAACAC CGGTATTACA ATCTATTCCC GGTGGCGCTG CAGCAAGACC TTTCATTACA CACCACAATG CATTGGATAT TCCATTATAC CTTCGTATTG CAAACGAATT ATATCTGAAA AGATTGATCG TAGGTGGTTT TGATGGTGTT TATGAGTTTT CCAAGAACTT CCGTAATGAA GGGATGGACA GAACACACAA TCCGGAATTT ACCGCAATGG AAATTTATGT AGCTTATAAA GACTACAACT GGATGATGGA CTTTACTGAA AAGTTATTGG AACATTGTGC TATTGCTGTT AACGGAACTT CTAAAGCGAA CTTCGGAGAA TACGAAATTG ATTTTAAAGC ACCATACCCA AGAGTTTCTA TGACGGAAGC TATTCAGAAA TATACGGGCT ATGATATTAC CGGGAAAACT GAAGAAGAAC TTCGTGCTTT TGCGAAGTCA ATTGGTCTTG ATGTGGATGA AACAATGGGT AAAGGAAAAC TTATTGATGA AATCTTCGGA GAAAAATGTG AAGGAAACTT TATTCAGCCA ACGTTTATTA CCGATTATCC AGTAGAAATG TCTCCGCTAA CTAAAAAACA CAGAAGCAAA GAAGGTTTAA CAGAGCGTTT CGAGCTTATG GTTTGTGGTA AGGAGATTGC AAATGCTTAT TCAGAGCTTA ATGACCCGAT TGATCAGAGA GCGCGTTTTG AAGATCAGCT AAAGCTTTCT GAAAAAGGAG ATGATGAAGC AGGACAATTT ATTGATGAAG ATTTCCTTAG AGCGTTGGAA TACGGAATGC CACCAACATC CGGTTTAGGA ATCGGTATGG ACAGATTGAT TATGTTCTTA ACGAACAACC CATCTATTCA GGAAGTATTA TTCTTCCCGC AGATGCGTCC GGAGAAAACT GAACCTAAGA TTGAGCTTAA TGAAGATGAG AAGGCAATTA TTGAAATTGT AGGCGCTCAG GAAGAACCAA TGCAGCTTTC GGAAGTGAAA GATAAAAGCC AGTTATCCGG TAAGAAATGG GATAAGTCTA TAAAGAATCT TACTAAAAAT AATCTGGTAA AAGTTGAGAA AGTAGATGAG GTTTTACTAA TTGCATTAGT ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT