SSGCID: Target Page

ElmeA.00164.a

Arginine--tRNA ligase

CENTER ID:	ElmeA.00164.a
ORGANISM:	Elizabethkingia meningoseptica ATCC 13253 (NBRC 12535)
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElmeA.00164.a.B1.GE42441	Full length(ElmeA.00164.a)		1	587

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
UniProt:	R9CGA2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEIKKQIQDQ ITEIIQRKYQ LEGIALEVQQ NKTDFEGDFT VVIFPLVKQA RKSPDALGQE LGEELQATTD YIRSYQVVKG FLNLSLNNDF FINQVQQLGE DLGNLPVKGE TIMVEYSSPN TNKPLHLGHV RNNLLGYSVA QILKAAGYNV IKTQIINDRG IHICKSMLAW EKFGNGETPE STGLKGDKLV GNYYVRFDQE YKKQIEELKA QGLTEEDAKK QAPIMQEAQK MLLDWENGDE KVRELWEMMN NWVYKGFNAS YSRMGVDFDQ RQYESQTYIL GKDLIQEGLN KGILFRKDDG SVWIDLTEDG LDQKLLLRSD GTSVYMTQDL GTAVERFKDN DIQQLIYTVG NEQDYHFQVL FLILKKLGYS WAENLHHLSY GMVELPHGKM KSREGTVVDA DELMEEMYVT AKEKAQELGK MENLTEEEKE TSYETVGLGA LKYYILKIDP KKKILFNPAE SIDFNGNTGP FIQYTYARIQ SLLNRAEYQE KSVSDNYQLN ASEKELLVMT TQFNEVIAKA AETLSPAQVA NYVYDLVKSY NSFYQNNPIL NQDNEDAKQL RLKLSKVTGN IIRKALNLLG IGTVNRM

NT Sequence

ATGGAAATAA AAAAACAGAT TCAGGATCAG ATTACTGAAA TTATTCAGCG AAAATACCAG TTAGAAGGTA TTGCACTAGA AGTTCAGCAA AATAAAACCG ATTTTGAAGG AGATTTCACT GTAGTGATTT TTCCGCTTGT AAAGCAGGCG AGGAAAAGTC CGGATGCTTT GGGGCAGGAA TTGGGAGAAG AATTGCAGGC TACTACTGAT TATATCAGGT CTTATCAGGT GGTAAAAGGT TTCCTTAACC TTTCTCTGAA TAATGATTTT TTTATTAACC AGGTACAGCA ACTGGGTGAG GATTTAGGAA ATCTGCCTGT GAAGGGAGAA ACAATTATGG TGGAATATTC TTCACCAAAT ACAAATAAGC CGTTACACTT AGGACATGTA CGTAATAATC TGTTAGGATA TTCTGTTGCT CAGATTTTAA AAGCTGCGGG TTATAATGTG ATCAAAACAC AGATTATTAA TGACCGTGGA ATCCATATTT GTAAATCCAT GTTGGCCTGG GAAAAATTTG GTAACGGAGA AACGCCGGAA TCGACAGGTT TAAAAGGAGA TAAGTTGGTC GGGAATTACT ATGTAAGATT CGATCAGGAA TATAAAAAAC AGATTGAGGA GCTAAAAGCT CAGGGACTAA CAGAAGAGGA TGCTAAAAAG CAGGCTCCGA TTATGCAGGA AGCTCAGAAA ATGTTACTGG ACTGGGAAAA TGGAGATGAA AAAGTTCGTG AGCTGTGGGA AATGATGAAC AATTGGGTTT ACAAAGGATT TAATGCCAGC TATTCCCGTA TGGGAGTTGA TTTCGATCAG CGCCAGTACG AAAGTCAGAC TTACATTTTA GGAAAAGATC TGATTCAGGA AGGTTTGAAC AAAGGAATTC TTTTCCGTAA AGATGACGGT TCTGTATGGA TTGACCTTAC TGAAGACGGG TTGGACCAGA AGTTATTACT ACGCTCTGAT GGTACTTCTG TTTACATGAC GCAGGATTTA GGAACAGCAG TAGAGCGTTT TAAAGATAAC GATATTCAGC AGTTAATATA TACCGTAGGA AATGAACAGG ATTATCATTT TCAGGTATTA TTCCTGATCC TTAAAAAACT GGGCTATTCA TGGGCTGAAA ATCTGCATCA CTTATCTTAC GGAATGGTAG AACTTCCACA TGGTAAGATG AAATCACGTG AAGGTACTGT GGTTGATGCT GATGAGCTGA TGGAGGAAAT GTATGTTACT GCAAAAGAAA AAGCACAGGA ACTGGGCAAA ATGGAAAACC TTACAGAAGA AGAAAAAGAG ACGTCTTATG AAACAGTAGG ACTTGGTGCT TTGAAATATT ATATTCTGAA GATTGATCCA AAGAAAAAAA TATTGTTCAA TCCGGCTGAA AGTATTGACT TTAATGGAAA TACAGGTCCT TTTATCCAGT ATACTTATGC CCGTATTCAG TCACTTCTTA ACAGAGCGGA ATATCAGGAG AAATCTGTTT CAGATAACTA TCAACTCAAT GCTTCTGAAA AAGAATTATT GGTCATGACA ACTCAGTTTA ATGAAGTAAT TGCTAAAGCA GCTGAAACAT TAAGTCCTGC ACAGGTTGCC AATTATGTTT ATGACCTGGT AAAATCTTAT AATTCCTTTT ATCAGAACAA TCCTATCCTT AATCAGGACA ATGAAGATGC AAAACAGCTC CGTTTGAAGC TGTCCAAAGT CACGGGTAAT ATTATCCGGA AAGCATTAAA CCTTTTAGGA ATAGGTACAG TTAACAGAAT G

Details for ElmeA.00164.a.B1.GE42441

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	68kDa
OBSERVED MW:	68kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHME IKKQIQDQIT EIIQRKYQLE GIALEVQQNK TDFEGDFTVV IFPLVKQARK SPDALGQELG EELQATTDYI RSYQVVKGFL NLSLNNDFFI NQVQQLGEDL GNLPVKGETI MVEYSSPNTN KPLHLGHVRN NLLGYSVAQI LKAAGYNVIK TQIINDRGIH ICKSMLAWEK FGNGETPEST GLKGDKLVGN YYVRFDQEYK KQIEELKAQG LTEEDAKKQA PIMQEAQKML LDWENGDEKV RELWEMMNNW VYKGFNASYS RMGVDFDQRQ YESQTYILGK DLIQEGLNKG ILFRKDDGSV WIDLTEDGLD QKLLLRSDGT SVYMTQDLGT AVERFKDNDI QQLIYTVGNE QDYHFQVLFL ILKKLGYSWA ENLHHLSYGM VELPHGKMKS REGTVVDADE LMEEMYVTAK EKAQELGKME NLTEEEKETS YETVGLGALK YYILKIDPKK KILFNPAESI DFNGNTGPFI QYTYARIQSL LNRAEYQEKS VSDNYQLNAS EKELLVMTTQ FNEVIAKAAE TLSPAQVANY VYDLVKSYNS FYQNNPILNQ DNEDAKQLRL KLSKVTGNII RKALNLLGIG TVNRM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa taaaaaaaca gattcaggat cagattactg aaattattca gcgaaaatac cagttagaag gtattgcact agaagttcag caaaataaaa ccgattttga aggagatttc actgtagtga tttttccgct tgtaaagcag gcgaggaaaa gtccggatgc tttggggcag gaattgggag aagaattgca ggctactact gattatatca ggtcttatca ggtggtaaaa ggtttcctta acctttctct gaataatgat ttttttatta accaggtaca gcaactgggt gaggatttag gaaatctgcc tgtgaaggga gaaacaatta tggtggaata ttcttcacca aatacaaata agccgttaca cttaggacat gtacgtaata atctgttagg atattctgtt gctcagattt taaaagctgc gggttataat gtgatcaaaa cacagattat taatgaccgt ggaatccata tttgtaaatc catgttggcc tgggaaaaat ttggtaacgg agaaacgccg gaatcgacag gtttaaaagg agataagttg gtcgggaatt actatgtaag attcgatcag gaatataaaa aacagattga ggagctaaaa gctcagggac taacagaaga ggatgctaaa aagcaggctc cgattatgca ggaagctcag aaaatgttac tggactggga aaatggagat gaaaaagttc gtgagctgtg ggaaatgatg aacaattggg tttacaaagg atttaatgcc agctattccc gtatgggagt tgatttcgat cagcgccagt acgaaagtca gacttacatt ttaggaaaag atctgattca ggaaggtttg aacaaaggaa ttcttttccg taaagatgac ggttctgtat ggattgacct tactgaagac gggttggacc agaagttatt actacgctct gatggtactt ctgtttacat gacgcaggat ttaggaacag cagtagagcg ttttaaagat aacgatattc agcagttaat atataccgta ggaaatgaac aggattatca ttttcaggta ttattcctga tccttaaaaa actgggctat tcatgggctg aaaatctgca tcacttatct tacggaatgg tagaacttcc acatggtaag atgaaatcac gtgaaggtac tgtggttgat gctgatgagc tgatggagga aatgtatgtt actgcaaaag aaaaagcaca ggaactgggc aaaatggaaa accttacaga agaagaaaaa gagacgtctt atgaaacagt aggacttggt gctttgaaat attatattct gaagattgat ccaaagaaaa aaatattgtt caatccggct gaaagtattg actttaatgg aaatacaggt ccttttatcc agtatactta tgcccgtatt cagtcacttc ttaacagagc ggaatatcag gagaaatctg tttcagataa ctatcaactc aatgcttctg aaaaagaatt attggtcatg acaactcagt ttaatgaagt aattgctaaa gcagctgaaa cattaagtcc tgcacaggtt gccaattatg tttatgacct ggtaaaatct tataattcct tttatcagaa caatcctatc cttaatcagg acaatgaaga tgcaaaacag ctccgtttga agctgtccaa agtcacgggt aatattatcc ggaaagcatt aaacctttta ggaataggta cagttaacag aatgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat