ElanA.19255.a

Aconitate hydratase

CENTER ID: ElanA.19255.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.19255.a.B1.GE40678 Full length(ElanA.19255.a) 1 754
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.19255.a.B1.PS38407 Full length(ElanA.19255.a) 1 754

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.369
UniProt: A0A077EMD2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTFDLDMIKK VYSDFDNRVN AAREFMGRPL TYSEKILCSH LFSTQQPEAF KRGESYVDFA PDRVAMQDAT AQMALLQFMQ AGKKKVAVPS TVHADHLIQA RVGADKDLQE AENKNNEVYQ FLQSVSNKYG IGFWKPGAGI IHQVVLENYA FPGGLMIGTD SHTVNAGGLG MVAIGVGGAD AVDVMAGMAW ELKFPKMIGV KLTGKLSGWC SPKDVILKVA GILTVKGGTG AIVEYFGEGA ESLSCTGKGT ICNMGAEIGA TTSIFEFDKN MAKYLRATGR EAVAAGAEAI ADNLRADKEV YADPAKYFDQ VIEINLDELE PALNGPFTPD LYTPISQMKE LAEKNGWPTK VEVGLIGSCT NSSYEDISRA ASIAKQAIDK NLVTAAEYTI TPGSELVRYT VERDGFLDTF AQIGGKVFAN ACGPCIGQWA REGSEKQEKN TIVHSFNRNF SKRADGNPNT YAFVGSPELV TALAIAGKLD FDPRTDKLIN KDGVEVLLDE PQGYELPIKG FDVEDAGYIA PAEDGSNVEV IVAPTSDRLQ LLEAFTPWDG KNITGAKLLI KAFGKCTTDH ISMAGPWLKY RGHLDNISNN MLIGAVNAYN METNNVKNEL NGEHMEVPAS ARAYKAAGVP TIVVGDENYG EGSSREHAAM EPRHLGVRAV LVKSFARIHE TNLKKQGMLG LTFADKADYD KIQEDDTINF LDLDQFAPGK PLTLEFVHAD GSKDIILANH TYNAGQIEWF KAGSALNLIK QQGK
NT Sequence
ATGACCTTTG ATTTGGATAT GATCAAAAAA GTTTATTCTG ATTTCGACAA CAGAGTAAAC GCAGCAAGAG AGTTTATGGG AAGACCTCTT ACTTATTCAG AGAAAATTTT ATGTTCCCAC TTATTTTCCA CTCAACAGCC AGAAGCTTTC AAACGCGGAG AATCTTATGT AGATTTCGCT CCGGACAGAG TTGCTATGCA GGACGCTACA GCACAGATGG CTTTACTACA GTTTATGCAA GCTGGTAAGA AGAAAGTTGC GGTTCCTTCT ACGGTACACG CAGACCACCT TATCCAGGCT AGAGTTGGAG CAGACAAAGA CCTTCAAGAA GCAGAAAATA AAAACAACGA AGTTTATCAA TTCCTTCAGT CAGTTTCTAA TAAATATGGT ATCGGATTCT GGAAGCCGGG AGCTGGTATT ATTCACCAGG TAGTTTTGGA AAACTATGCA TTCCCTGGAG GATTGATGAT TGGTACTGAC TCTCATACTG TAAATGCAGG TGGTTTAGGT ATGGTTGCAA TCGGTGTTGG TGGTGCTGAT GCTGTGGATG TGATGGCCGG AATGGCATGG GAGCTTAAAT TCCCTAAAAT GATCGGTGTA AAATTAACCG GTAAACTTAG CGGATGGTGT TCTCCTAAAG ATGTAATTCT GAAAGTTGCT GGTATCCTTA CTGTAAAAGG TGGTACTGGT GCTATTGTAG AATACTTCGG AGAAGGTGCT GAATCACTTT CTTGTACTGG TAAAGGTACA ATCTGTAACA TGGGTGCAGA GATCGGAGCA ACAACTTCTA TCTTTGAATT CGATAAAAAC ATGGCTAAAT ACCTAAGAGC TACAGGAAGA GAAGCTGTTG CTGCAGGTGC TGAGGCTATT GCTGATAACC TAAGAGCTGA TAAAGAAGTT TATGCAGATC CTGCTAAATA CTTTGATCAG GTTATTGAAA TCAACCTGGA TGAATTAGAG CCGGCATTAA ACGGTCCTTT CACTCCGGAT TTATATACTC CAATTTCTCA GATGAAAGAA CTTGCTGAGA AAAACGGATG GCCGACTAAA GTTGAAGTTG GTTTGATCGG TTCTTGTACA AACTCTTCTT ATGAAGATAT CTCAAGAGCT GCTTCTATCG CTAAGCAAGC AATTGATAAA AACCTGGTAA CTGCTGCTGA ATATACTATT ACTCCGGGAT CCGAATTAGT AAGATATACA GTAGAAAGAG ATGGCTTCCT GGATACTTTT GCTCAAATTG GTGGTAAAGT ATTCGCTAAT GCTTGTGGAC CATGTATCGG TCAATGGGCT CGTGAAGGTT CAGAAAAACA AGAGAAAAAC ACAATCGTTC ACTCTTTCAA CAGAAACTTC TCTAAGAGAG CAGACGGTAA CCCGAATACT TATGCATTCG TAGGTTCTCC TGAATTGGTA ACTGCACTTG CTATTGCTGG TAAACTAGAT TTCGATCCAA GAACAGACAA ATTAATTAAT AAAGATGGTG TTGAAGTTCT TCTAGACGAG CCTCAAGGTT ATGAATTACC AATTAAAGGT TTCGACGTTG AAGACGCTGG TTATATCGCT CCTGCTGAAG ACGGTTCAAA TGTTGAGGTT ATTGTAGCTC CTACATCCGA CAGACTTCAG TTATTAGAGG CTTTTACTCC TTGGGATGGT AAAAACATTA CAGGTGCTAA ATTATTGATC AAAGCATTTG GTAAGTGTAC AACTGACCAC ATCTCTATGG CTGGTCCATG GTTGAAGTAC AGAGGTCACT TAGATAATAT TTCTAACAAC ATGTTGATCG GAGCTGTTAA TGCTTACAAC ATGGAAACTA ACAATGTGAA GAACGAATTA AACGGAGAGC ATATGGAAGT TCCTGCATCT GCAAGAGCTT ACAAGGCTGC CGGAGTTCCA ACAATTGTTG TTGGTGATGA AAACTACGGT GAAGGTTCTT CAAGAGAGCA CGCTGCAATG GAGCCTCGTC ACTTAGGTGT TAGAGCTGTA TTAGTTAAAT CTTTCGCTCG TATCCACGAG ACTAACCTTA AAAAACAAGG TATGTTAGGA TTAACTTTTG CTGACAAAGC AGATTATGAC AAAATTCAGG AAGATGATAC AATCAACTTC TTAGATCTTG ATCAGTTTGC TCCAGGTAAG CCATTAACAT TAGAGTTTGT TCACGCTGAT GGTTCTAAAG ATATTATTCT TGCTAACCAT ACTTACAACG CAGGACAAAT CGAGTGGTTC AAAGCTGGAT CTGCGCTTAA CCTAATCAAG CAACAAGGGA AA
Details for ElanA.19255.a.B1.GE40678
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 83kDa
OBSERVED MW: 84kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMT FDLDMIKKVY SDFDNRVNAA REFMGRPLTY SEKILCSHLF STQQPEAFKR GESYVDFAPD RVAMQDATAQ MALLQFMQAG KKKVAVPSTV HADHLIQARV GADKDLQEAE NKNNEVYQFL QSVSNKYGIG FWKPGAGIIH QVVLENYAFP GGLMIGTDSH TVNAGGLGMV AIGVGGADAV DVMAGMAWEL KFPKMIGVKL TGKLSGWCSP KDVILKVAGI LTVKGGTGAI VEYFGEGAES LSCTGKGTIC NMGAEIGATT SIFEFDKNMA KYLRATGREA VAAGAEAIAD NLRADKEVYA DPAKYFDQVI EINLDELEPA LNGPFTPDLY TPISQMKELA EKNGWPTKVE VGLIGSCTNS SYEDISRAAS IAKQAIDKNL VTAAEYTITP GSELVRYTVE RDGFLDTFAQ IGGKVFANAC GPCIGQWARE GSEKQEKNTI VHSFNRNFSK RADGNPNTYA FVGSPELVTA LAIAGKLDFD PRTDKLINKD GVEVLLDEPQ GYELPIKGFD VEDAGYIAPA EDGSNVEVIV APTSDRLQLL EAFTPWDGKN ITGAKLLIKA FGKCTTDHIS MAGPWLKYRG HLDNISNNML IGAVNAYNME TNNVKNELNG EHMEVPASAR AYKAAGVPTI VVGDENYGEG SSREHAAMEP RHLGVRAVLV KSFARIHETN LKKQGMLGLT FADKADYDKI QEDDTINFLD LDQFAPGKPL TLEFVHADGS KDIILANHTY NAGQIEWFKA GSALNLIKQQ GK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacct ttgatttgga tatgatcaaa aaagtttatt ctgatttcga caacagagta aacgcagcaa gagagtttat gggaagacct cttacttatt cagagaaaat tttatgttcc cacttatttt ccactcaaca gccagaagct ttcaaacgcg gagaatctta tgtagatttc gctccggaca gagttgctat gcaggacgct acagcacaga tggctttact acagtttatg caagctggta agaagaaagt tgcggttcct tctacggtac acgcagacca ccttatccag gctagagttg gagcagacaa agaccttcaa gaagcagaaa ataaaaacaa cgaagtttat caattccttc agtcagtttc taataaatat ggtatcggat tctggaagcc gggagctggt attattcacc aggtagtttt ggaaaactat gcattccctg gaggattgat gattggtact gactctcata ctgtaaatgc aggtggttta ggtatggttg caatcggtgt tggtggtgct gatgctgtgg atgtgatggc cggaatggca tgggagctta aattccctaa aatgatcggt gtaaaattaa ccggtaaact tagcggatgg tgttctccta aagatgtaat tctgaaagtt gctggtatcc ttactgtaaa aggtggtact ggtgctattg tagaatactt cggagaaggt gctgaatcac tttcttgtac tggtaaaggt acaatctgta acatgggtgc agagatcgga gcaacaactt ctatctttga attcgataaa aacatggcta aatacctaag agctacagga agagaagctg ttgctgcagg tgctgaggct attgctgata acctaagagc tgataaagaa gtttatgcag atcctgctaa atactttgat caggttattg aaatcaacct ggatgaatta gagccggcat taaacggtcc tttcactccg gatttatata ctccaatttc tcagatgaaa gaacttgctg agaaaaacgg atggccgact aaagttgaag ttggtttgat cggttcttgt acaaactctt cttatgaaga tatctcaaga gctgcttcta tcgctaagca agcaattgat aaaaacctgg taactgctgc tgaatatact attactccgg gatccgaatt agtaagatat acagtagaaa gagatggctt cctggatact tttgctcaaa ttggtggtaa agtattcgct aatgcttgtg gaccatgtat cggtcaatgg gctcgtgaag gttcagaaaa acaagagaaa aacacaatcg ttcactcttt caacagaaac ttctctaaga gagcagacgg taacccgaat acttatgcat tcgtaggttc tcctgaattg gtaactgcac ttgctattgc tggtaaacta gatttcgatc caagaacaga caaattaatt aataaagatg gtgttgaagt tcttctagac gagcctcaag gttatgaatt accaattaaa ggtttcgacg ttgaagacgc tggttatatc gctcctgctg aagacggttc aaatgttgag gttattgtag ctcctacatc cgacagactt cagttattag aggcttttac tccttgggat ggtaaaaaca ttacaggtgc taaattattg atcaaagcat ttggtaagtg tacaactgac cacatctcta tggctggtcc atggttgaag tacagaggtc acttagataa tatttctaac aacatgttga tcggagctgt taatgcttac aacatggaaa ctaacaatgt gaagaacgaa ttaaacggag agcatatgga agttcctgca tctgcaagag cttacaaggc tgccggagtt ccaacaattg ttgttggtga tgaaaactac ggtgaaggtt cttcaagaga gcacgctgca atggagcctc gtcacttagg tgttagagct gtattagtta aatctttcgc tcgtatccac gagactaacc ttaaaaaaca aggtatgtta ggattaactt ttgctgacaa agcagattat gacaaaattc aggaagatga tacaatcaac ttcttagatc ttgatcagtt tgctccaggt aagccattaa cattagagtt tgttcacgct gatggttcta aagatattat tcttgctaac catacttaca acgcaggaca aatcgagtgg ttcaaagctg gatctgcgct taacctaatc aagcaacaag ggaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for ElanA.19255.a.B1.PS38407
PURIFICATION DATe: 1/30/2018
CONCENTRATION: 16.18mg/ml
OBSERVED MW: 82kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMT FDLDMIKKVY SDFDNRVNAA REFMGRPLTY SEKILCSHLF STQQPEAFKR GESYVDFAPD RVAMQDATAQ MALLQFMQAG KKKVAVPSTV HADHLIQARV GADKDLQEAE NKNNEVYQFL QSVSNKYGIG FWKPGAGIIH QVVLENYAFP GGLMIGTDSH TVNAGGLGMV AIGVGGADAV DVMAGMAWEL KFPKMIGVKL TGKLSGWCSP KDVILKVAGI LTVKGGTGAI VEYFGEGAES LSCTGKGTIC NMGAEIGATT SIFEFDKNMA KYLRATGREA VAAGAEAIAD NLRADKEVYA DPAKYFDQVI EINLDELEPA LNGPFTPDLY TPISQMKELA EKNGWPTKVE VGLIGSCTNS SYEDISRAAS IAKQAIDKNL VTAAEYTITP GSELVRYTVE RDGFLDTFAQ IGGKVFANAC GPCIGQWARE GSEKQEKNTI VHSFNRNFSK RADGNPNTYA FVGSPELVTA LAIAGKLDFD PRTDKLINKD GVEVLLDEPQ GYELPIKGFD VEDAGYIAPA EDGSNVEVIV APTSDRLQLL EAFTPWDGKN ITGAKLLIKA FGKCTTDHIS MAGPWLKYRG HLDNISNNML IGAVNAYNME TNNVKNELNG EHMEVPASAR AYKAAGVPTI VVGDENYGEG SSREHAAMEP RHLGVRAVLV KSFARIHETN LKKQGMLGLT FADKADYDKI QEDDTINFLD LDQFAPGKPL TLEFVHADGS KDIILANHTY NAGQIEWFKA GSALNLIKQQ GK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCT TTGATTTGGA TATGATCAAA AAAGTTTATT CTGATTTCGA CAACAGAGTA AACGCAGCAA GAGAGTTTAT GGGAAGACCT CTTACTTATT CAGAGAAAAT TTTATGTTCC CACTTATTTT CCACTCAACA GCCAGAAGCT TTCAAACGCG GAGAATCTTA TGTAGATTTC GCTCCGGACA GAGTTGCTAT GCAGGACGCT ACAGCACAGA TGGCTTTACT ACAGTTTATG CAAGCTGGTA AGAAGAAAGT TGCGGTTCCT TCTACGGTAC ACGCAGACCA CCTTATCCAG GCTAGAGTTG GAGCAGACAA AGACCTTCAA GAAGCAGAAA ATAAAAACAA CGAAGTTTAT CAATTCCTTC AGTCAGTTTC TAATAAATAT GGTATCGGAT TCTGGAAGCC GGGAGCTGGT ATTATTCACC AGGTAGTTTT GGAAAACTAT GCATTCCCTG GAGGATTGAT GATTGGTACT GACTCTCATA CTGTAAATGC AGGTGGTTTA GGTATGGTTG CAATCGGTGT TGGTGGTGCT GATGCTGTGG ATGTGATGGC CGGAATGGCA TGGGAGCTTA AATTCCCTAA AATGATCGGT GTAAAATTAA CCGGTAAACT TAGCGGATGG TGTTCTCCTA AAGATGTAAT TCTGAAAGTT GCTGGTATCC TTACTGTAAA AGGTGGTACT GGTGCTATTG TAGAATACTT CGGAGAAGGT GCTGAATCAC TTTCTTGTAC TGGTAAAGGT ACAATCTGTA ACATGGGTGC AGAGATCGGA GCAACAACTT CTATCTTTGA ATTCGATAAA AACATGGCTA AATACCTAAG AGCTACAGGA AGAGAAGCTG TTGCTGCAGG TGCTGAGGCT ATTGCTGATA ACCTAAGAGC TGATAAAGAA GTTTATGCAG ATCCTGCTAA ATACTTTGAT CAGGTTATTG AAATCAACCT GGATGAATTA GAGCCGGCAT TAAACGGTCC TTTCACTCCG GATTTATATA CTCCAATTTC TCAGATGAAA GAACTTGCTG AGAAAAACGG ATGGCCGACT AAAGTTGAAG TTGGTTTGAT CGGTTCTTGT ACAAACTCTT CTTATGAAGA TATCTCAAGA GCTGCTTCTA TCGCTAAGCA AGCAATTGAT AAAAACCTGG TAACTGCTGC TGAATATACT ATTACTCCGG GATCCGAATT AGTAAGATAT ACAGTAGAAA GAGATGGCTT CCTGGATACT TTTGCTCAAA TTGGTGGTAA AGTATTCGCT AATGCTTGTG GACCATGTAT CGGTCAATGG GCTCGTGAAG GTTCAGAAAA ACAAGAGAAA AACACAATCG TTCACTCTTT CAACAGAAAC TTCTCTAAGA GAGCAGACGG TAACCCGAAT ACTTATGCAT TCGTAGGTTC TCCTGAATTG GTAACTGCAC TTGCTATTGC TGGTAAACTA GATTTCGATC CAAGAACAGA CAAATTAATT AATAAAGATG GTGTTGAAGT TCTTCTAGAC GAGCCTCAAG GTTATGAATT ACCAATTAAA GGTTTCGACG TTGAAGACGC TGGTTATATC GCTCCTGCTG AAGACGGTTC AAATGTTGAG GTTATTGTAG CTCCTACATC CGACAGACTT CAGTTATTAG AGGCTTTTAC TCCTTGGGAT GGTAAAAACA TTACAGGTGC TAAATTATTG ATCAAAGCAT TTGGTAAGTG TACAACTGAC CACATCTCTA TGGCTGGTCC ATGGTTGAAG TACAGAGGTC ACTTAGATAA TATTTCTAAC AACATGTTGA TCGGAGCTGT TAATGCTTAC AACATGGAAA CTAACAATGT GAAGAACGAA TTAAACGGAG AGCATATGGA AGTTCCTGCA TCTGCAAGAG CTTACAAGGC TGCCGGAGTT CCAACAATTG TTGTTGGTGA TGAAAACTAC GGTGAAGGTT CTTCAAGAGA GCACGCTGCA ATGGAGCCTC GTCACTTAGG TGTTAGAGCT GTATTAGTTA AATCTTTCGC TCGTATCCAC GAGACTAACC TTAAAAAACA AGGTATGTTA GGATTAACTT TTGCTGACAA AGCAGATTAT GACAAAATTC AGGAAGATGA TACAATCAAC TTCTTAGATC TTGATCAGTT TGCTCCAGGT AAGCCATTAA CATTAGAGTT TGTTCACGCT GATGGTTCTA AAGATATTAT TCTTGCTAAC CATACTTACA ACGCAGGACA AATCGAGTGG TTCAAAGCTG GATCTGCGCT TAACCTAATC AAGCAACAAG GGAAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT