ElanA.19249.a

Methylmalonyl-CoA mutase

CENTER ID: ElanA.19249.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.19249.a.B1.GE40677 Full length(ElanA.19249.a) 1 707
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.311
UniProt: A0A077EJ96

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRKHITTPKP DFDIKPSAFE NYNFEKDGLQ MKTSYSADDA RALIDKEYSA GIAPYLRGPY TTMYVQKPWT IRQYAGFSTA EESNAFYRRN LAAGQKGLSV AFDLATHRGY DSDHARVVGD VGKAGVAIDS VEDMKILFNE IPLDQISVSM TMNGAVLPIL AFYIVAAEEQ GVNQELLSGT IQNDILKEFM VRNTYIYPPA PSMKIIADIF EYTSQNIPKF NSISISGYHM QEAGATPVLE MAYTLADGLE YVRTGIQAGM NIDDFAPRLS FFWAIGMNHF MEIAKMRAAR YIWSELIAQF KPQNSKSLAL RTHSQTSGWS LTEQEPFNNI TRTAIEALSS ALGGTQSLHT NALDEAIALP TDYSAKIARN TQIILQQESK ICDVVDPMGG SYLVESLTQQ MIDEAMKYID EVEKEGGMTK AIEAGIPKMR IEEAAAKKQA KIDSSEEFII GVNSFRTQQK QPQFEILDID NSLVRQKQIE RLNTIKTERD NQAVEQILDE IKECAKTGKG NLLALSIEAA RRRATLGEIS DALEGEFGRY KAQIKTIQGV YAMNASKNEY FAKAVELTQK FEEYEGRRPR LMVVKMGQDG HDRGAKVVAT AFADMGFDVD VAPLFQTPEE VAKQAVENDI HILGVSSLAA GHKTLVPQVV EELKKLGAED ITIVVGGVIP QQDYEFLYAN GADHIFGPGT NLPQCACEIL EKLIVTE
NT Sequence
ATGCGAAAAC ATATAACGAC GCCGAAACCT GATTTTGATA TTAAGCCTTC AGCATTTGAA AATTATAATT TTGAGAAGGA CGGACTTCAG ATGAAGACTT CGTATTCTGC AGACGATGCC CGGGCTTTGA TTGATAAAGA ATATTCGGCA GGGATTGCTC CTTATCTCAG AGGTCCATAT ACAACAATGT ATGTTCAGAA ACCATGGACA ATCAGGCAAT ATGCAGGATT CTCTACTGCA GAAGAATCCA ATGCATTTTA CCGTAGAAAT CTTGCAGCAG GTCAAAAAGG ACTTTCTGTA GCATTTGATC TTGCAACGCA CAGAGGATAT GACTCGGATC ATGCAAGAGT TGTTGGTGAT GTTGGAAAAG CCGGAGTGGC AATAGATTCA GTAGAGGACA TGAAGATTCT CTTCAATGAA ATTCCTCTGG ATCAGATTTC CGTTTCCATG ACCATGAACG GTGCTGTGTT GCCTATTTTG GCATTTTATA TTGTAGCGGC AGAAGAGCAA GGCGTAAATC AGGAGCTTCT TTCGGGAACC ATCCAGAATG ATATTCTGAA AGAGTTTATG GTGAGAAATA CCTATATCTA TCCACCAGCA CCATCTATGA AGATTATTGC CGATATATTC GAATATACTT CACAGAATAT TCCTAAATTC AATTCTATTT CTATTTCAGG TTACCATATG CAGGAAGCAG GAGCGACACC TGTACTGGAA ATGGCTTATA CACTGGCTGA CGGGCTGGAG TATGTAAGAA CCGGAATACA GGCCGGAATG AATATTGATG ATTTTGCACC ACGCTTATCA TTCTTTTGGG CTATTGGTAT GAATCATTTT ATGGAGATTG CCAAAATGCG TGCGGCCAGA TATATTTGGT CCGAACTGAT TGCGCAGTTC AAACCTCAAA ATTCAAAATC TCTTGCTCTA AGAACGCATT CCCAAACTTC GGGATGGAGC TTAACAGAAC AAGAACCGTT TAATAATATT ACCCGTACTG CTATCGAAGC ATTATCTTCA GCATTAGGCG GGACACAATC TTTGCATACC AATGCACTGG ATGAGGCTAT TGCTTTGCCT ACAGATTATT CTGCTAAAAT TGCCAGAAAT ACACAGATTA TATTGCAGCA GGAAAGCAAA ATATGTGATG TGGTAGATCC GATGGGTGGA AGTTATCTTG TAGAAAGTCT TACCCAGCAG ATGATTGATG AAGCCATGAA GTATATTGAT GAGGTAGAGA AAGAAGGTGG GATGACTAAG GCTATTGAAG CCGGTATTCC AAAAATGAGA ATCGAGGAAG CGGCTGCTAA AAAGCAGGCA AAGATTGACA GTAGTGAAGA ATTTATCATC GGGGTAAACA GCTTCAGAAC TCAGCAGAAG CAACCACAAT TTGAAATTCT GGATATTGAT AATTCATTGG TGCGCCAGAA GCAGATTGAA AGACTGAATA CTATAAAAAC TGAAAGAGAT AATCAGGCCG TAGAGCAGAT TCTCGATGAG ATTAAAGAAT GTGCAAAAAC GGGAAAAGGA AATCTCCTGG CGCTGAGTAT AGAAGCTGCA AGAAGAAGAG CTACTTTAGG CGAAATTTCT GATGCTTTGG AAGGTGAATT CGGAAGATAT AAAGCACAAA TAAAAACTAT TCAGGGAGTT TACGCTATGA ATGCATCTAA AAATGAATAT TTTGCTAAAG CTGTTGAGCT GACACAAAAG TTTGAAGAAT ACGAAGGACG CAGGCCGCGT TTAATGGTTG TGAAAATGGG GCAGGATGGC CACGATAGAG GTGCAAAAGT AGTAGCTACA GCTTTTGCAG ATATGGGCTT TGATGTGGAT GTTGCACCAT TATTCCAGAC ACCGGAAGAA GTGGCGAAAC AGGCAGTGGA AAATGATATT CATATTCTGG GTGTCTCTTC ACTTGCTGCA GGACACAAAA CGCTGGTTCC ACAGGTAGTG GAAGAATTGA AAAAATTAGG TGCAGAAGAT ATTACCATTG TTGTAGGTGG TGTTATCCCT CAACAGGATT ATGAATTCTT ATACGCAAAC GGAGCGGATC ATATATTTGG TCCCGGAACA AACCTGCCGC AATGTGCATG TGAGATTCTG GAGAAACTGA TTGTTACGGA A
Details for ElanA.19249.a.B1.GE40677
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 79kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMR KHITTPKPDF DIKPSAFENY NFEKDGLQMK TSYSADDARA LIDKEYSAGI APYLRGPYTT MYVQKPWTIR QYAGFSTAEE SNAFYRRNLA AGQKGLSVAF DLATHRGYDS DHARVVGDVG KAGVAIDSVE DMKILFNEIP LDQISVSMTM NGAVLPILAF YIVAAEEQGV NQELLSGTIQ NDILKEFMVR NTYIYPPAPS MKIIADIFEY TSQNIPKFNS ISISGYHMQE AGATPVLEMA YTLADGLEYV RTGIQAGMNI DDFAPRLSFF WAIGMNHFME IAKMRAARYI WSELIAQFKP QNSKSLALRT HSQTSGWSLT EQEPFNNITR TAIEALSSAL GGTQSLHTNA LDEAIALPTD YSAKIARNTQ IILQQESKIC DVVDPMGGSY LVESLTQQMI DEAMKYIDEV EKEGGMTKAI EAGIPKMRIE EAAAKKQAKI DSSEEFIIGV NSFRTQQKQP QFEILDIDNS LVRQKQIERL NTIKTERDNQ AVEQILDEIK ECAKTGKGNL LALSIEAARR RATLGEISDA LEGEFGRYKA QIKTIQGVYA MNASKNEYFA KAVELTQKFE EYEGRRPRLM VVKMGQDGHD RGAKVVATAF ADMGFDVDVA PLFQTPEEVA KQAVENDIHI LGVSSLAAGH KTLVPQVVEE LKKLGAEDIT IVVGGVIPQQ DYEFLYANGA DHIFGPGTNL PQCACEILEK LIVTE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgaa aacatataac gacgccgaaa cctgattttg atattaagcc ttcagcattt gaaaattata attttgagaa ggacggactt cagatgaaga cttcgtattc tgcagacgat gcccgggctt tgattgataa agaatattcg gcagggattg ctccttatct cagaggtcca tatacaacaa tgtatgttca gaaaccatgg acaatcaggc aatatgcagg attctctact gcagaagaat ccaatgcatt ttaccgtaga aatcttgcag caggtcaaaa aggactttct gtagcatttg atcttgcaac gcacagagga tatgactcgg atcatgcaag agttgttggt gatgttggaa aagccggagt ggcaatagat tcagtagagg acatgaagat tctcttcaat gaaattcctc tggatcagat ttccgtttcc atgaccatga acggtgctgt gttgcctatt ttggcatttt atattgtagc ggcagaagag caaggcgtaa atcaggagct tctttcggga accatccaga atgatattct gaaagagttt atggtgagaa atacctatat ctatccacca gcaccatcta tgaagattat tgccgatata ttcgaatata cttcacagaa tattcctaaa ttcaattcta tttctatttc aggttaccat atgcaggaag caggagcgac acctgtactg gaaatggctt atacactggc tgacgggctg gagtatgtaa gaaccggaat acaggccgga atgaatattg atgattttgc accacgctta tcattctttt gggctattgg tatgaatcat tttatggaga ttgccaaaat gcgtgcggcc agatatattt ggtccgaact gattgcgcag ttcaaacctc aaaattcaaa atctcttgct ctaagaacgc attcccaaac ttcgggatgg agcttaacag aacaagaacc gtttaataat attacccgta ctgctatcga agcattatct tcagcattag gcgggacaca atctttgcat accaatgcac tggatgaggc tattgctttg cctacagatt attctgctaa aattgccaga aatacacaga ttatattgca gcaggaaagc aaaatatgtg atgtggtaga tccgatgggt ggaagttatc ttgtagaaag tcttacccag cagatgattg atgaagccat gaagtatatt gatgaggtag agaaagaagg tgggatgact aaggctattg aagccggtat tccaaaaatg agaatcgagg aagcggctgc taaaaagcag gcaaagattg acagtagtga agaatttatc atcggggtaa acagcttcag aactcagcag aagcaaccac aatttgaaat tctggatatt gataattcat tggtgcgcca gaagcagatt gaaagactga atactataaa aactgaaaga gataatcagg ccgtagagca gattctcgat gagattaaag aatgtgcaaa aacgggaaaa ggaaatctcc tggcgctgag tatagaagct gcaagaagaa gagctacttt aggcgaaatt tctgatgctt tggaaggtga attcggaaga tataaagcac aaataaaaac tattcaggga gtttacgcta tgaatgcatc taaaaatgaa tattttgcta aagctgttga gctgacacaa aagtttgaag aatacgaagg acgcaggccg cgtttaatgg ttgtgaaaat ggggcaggat ggccacgata gaggtgcaaa agtagtagct acagcttttg cagatatggg ctttgatgtg gatgttgcac cattattcca gacaccggaa gaagtggcga aacaggcagt ggaaaatgat attcatattc tgggtgtctc ttcacttgct gcaggacaca aaacgctggt tccacaggta gtggaagaat tgaaaaaatt aggtgcagaa gatattacca ttgttgtagg tggtgttatc cctcaacagg attatgaatt cttatacgca aacggagcgg atcatatatt tggtcccgga acaaacctgc cgcaatgtgc atgtgagatt ctggagaaac tgattgttac ggaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat