ElanA.18890.a

Phenylalanine--tRNA ligase beta subunit

CENTER ID: ElanA.18890.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.18890.a.B1.GE41097 Full length(ElanA.18890.a) 1 799
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.1990
UniProt: A0A077EC82

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKISDKWLRT HLETDLSAEK LGIILTDLGL EVEGIDPYES VKGSLNGVVV GKVLTCEQHP NADKLKVTTV DINTGTPLHI VCGAPNVAAE QKVAVATIGT TIHLSNGDSF KIAKSKLRGE VSEGMLCAED ELGLGESHAG IMVLPEDYEI GKPLSDYIKV EQDEVYEIGL TPNRTDAMSH YGVARDLQAY LSLNKLKSTF NKLQAPVING EGSHNFKLEV EDTVLTPRYL GAVIEDIKVE ESPEWLKNRL KAIGLGPINN VVDITNYILH SYGQPLHAFD AAKINGSVVK VGVAPEGTKF KTLDGTERTL NGTEIMIKDG DNTPMCIAGV FGGENSGVSE GTTSVFLESA YFNPVAVRKA AKAHGLNTDA SFRFERGVDP NITKDALLKA IEMIQEIAGG KLKGEILESF PKKVDYFPVI LRYSKVDQIL GIKIHRETIK EILKSLDILV LNEIKDGLEI SVPPYRADVT REIDVIEEIL RVYGYNKVSS QDKIAFTPVR LTLDDQDSLE NFWARMLQSN GFNEVMNNSL TSLKEEKADA VTLLNPLSNE LSTMRQSLLE GLLQNADYNI KRKNQDIKFF ELGKIYFKKE KFEERKQLAI LVSGNNNPEN WMLSKSPTDF FILKGYVQLL LDKLGLNTTE KALEDARFSD ALEIVAEGKT IARIGIVSKA LLKEADLSQP AYYAEIELEA CQALRSTTNF KFVDIPKFNK IRRDLALLVD KTVSYNDLLQ ASNGISANLK KIQLFDVYEG KNLPEGKKSY ALSFELLNTE KTLEEAEIAN IMNKLIKKYQ KEFNAELRS
NT Sequence
ATGAAAATTT CTGATAAGTG GCTTAGAACT CACCTTGAAA CAGATTTGTC AGCTGAAAAG CTTGGAATAA TTCTTACAGA TTTAGGTCTG GAAGTGGAAG GAATAGATCC TTATGAATCT GTGAAAGGAA GTCTTAATGG AGTAGTGGTT GGAAAAGTGC TTACTTGTGA GCAGCATCCT AATGCAGATA AACTGAAAGT AACTACTGTA GACATTAATA CCGGAACACC TTTACATATC GTTTGTGGTG CACCGAATGT TGCTGCAGAA CAAAAGGTAG CTGTTGCTAC AATAGGTACT ACAATTCATC TGAGTAATGG TGATTCCTTT AAAATTGCTA AATCCAAACT TAGAGGAGAG GTATCTGAAG GAATGCTTTG CGCTGAAGAC GAATTAGGTC TTGGAGAAAG CCATGCCGGA ATTATGGTAT TGCCGGAAGA TTATGAAATT GGAAAACCTT TATCCGATTA TATTAAGGTA GAGCAGGATG AGGTTTATGA AATCGGACTT ACACCCAACA GAACAGATGC GATGTCGCAT TATGGTGTTG CCAGAGACCT TCAGGCTTAT TTATCTTTAA ATAAATTAAA ATCTACATTC AACAAGCTTC AGGCTCCTGT AATTAATGGA GAAGGAAGTC ATAACTTTAA ACTTGAAGTT GAAGATACAG TGTTAACACC TCGTTATTTA GGCGCTGTTA TCGAAGATAT TAAAGTTGAG GAATCTCCGG AGTGGTTAAA GAACCGTCTG AAAGCTATTG GCCTTGGACC AATTAATAAT GTTGTAGATA TTACCAACTA TATTTTACAT TCTTATGGGC AGCCTTTACA TGCTTTTGAT GCTGCAAAGA TCAATGGAAG TGTTGTAAAG GTAGGAGTGG CTCCTGAAGG AACGAAATTC AAAACTCTGG ATGGTACAGA GCGTACTTTA AATGGTACTG AAATTATGAT TAAAGACGGA GATAATACAC CAATGTGTAT TGCCGGAGTC TTTGGTGGTG AAAATTCAGG AGTTTCAGAA GGAACAACTT CAGTGTTCTT AGAAAGTGCA TATTTCAACC CTGTAGCAGT ACGTAAAGCA GCGAAGGCAC ATGGTCTAAA TACAGATGCT TCTTTCCGTT TCGAAAGAGG TGTAGATCCT AATATTACTA AAGATGCTTT ATTAAAAGCA ATAGAGATGA TTCAGGAGAT TGCAGGTGGA AAACTAAAAG GAGAAATCCT TGAATCTTTT CCTAAAAAAG TTGATTATTT TCCTGTTATC CTTAGGTATT CCAAAGTAGA CCAGATTCTG GGGATTAAGA TTCACAGAGA AACAATAAAA GAAATCCTGA AATCTCTTGA TATATTAGTT CTGAATGAGA TTAAAGACGG ATTGGAAATT TCTGTTCCGC CATACAGAGC CGATGTAACC AGAGAAATTG ATGTTATAGA AGAAATTCTT CGTGTTTACG GATATAACAA AGTAAGCTCT CAGGATAAAA TAGCATTTAC ACCAGTTCGT TTAACATTAG ATGATCAGGA TTCTCTGGAA AATTTCTGGG CAAGAATGTT ACAGTCTAAT GGCTTCAATG AAGTAATGAA TAACTCTCTG ACTTCTCTTA AAGAAGAGAA AGCAGATGCT GTTACCTTAC TGAATCCTTT GAGTAATGAA CTTAGCACAA TGCGCCAGTC TTTATTAGAA GGATTATTAC AGAATGCTGA TTACAATATC AAGCGTAAGA ATCAGGATAT TAAATTCTTT GAGCTTGGGA AAATCTACTT CAAAAAAGAG AAATTTGAAG AGCGCAAACA ATTGGCAATT CTTGTTTCCG GTAATAATAA TCCTGAAAAC TGGATGCTGA GTAAGTCTCC AACCGACTTT TTCATCCTGA AAGGTTATGT TCAGTTATTA CTGGATAAAT TAGGACTAAA TACGACTGAA AAAGCTTTAG AAGATGCACG CTTCTCAGAT GCATTAGAAA TTGTTGCTGA AGGAAAAACT ATTGCCAGAA TAGGTATTGT AAGCAAGGCT TTATTAAAAG AGGCAGATCT TTCTCAACCT GCTTATTATG CAGAAATAGA ATTAGAAGCT TGTCAGGCTT TAAGAAGCAC GACTAACTTT AAATTCGTAG ATATTCCGAA GTTTAATAAG ATAAGAAGGG ATTTAGCGTT ATTAGTAGAT AAAACTGTTT CTTATAACGA TTTGCTTCAG GCATCTAACG GTATTTCAGC TAATCTTAAA AAGATTCAGT TATTCGATGT TTACGAAGGT AAAAACCTTC CGGAAGGTAA AAAATCTTAT GCCCTAAGCT TTGAATTATT AAATACCGAG AAGACATTAG AAGAAGCAGA GATTGCAAAT ATTATGAATA AGCTGATCAA GAAGTATCAG AAAGAATTTA ATGCTGAACT GCGTTCA
Details for ElanA.18890.a.B1.GE41097
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 90kDa
OBSERVED MW: 90kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK ISDKWLRTHL ETDLSAEKLG IILTDLGLEV EGIDPYESVK GSLNGVVVGK VLTCEQHPNA DKLKVTTVDI NTGTPLHIVC GAPNVAAEQK VAVATIGTTI HLSNGDSFKI AKSKLRGEVS EGMLCAEDEL GLGESHAGIM VLPEDYEIGK PLSDYIKVEQ DEVYEIGLTP NRTDAMSHYG VARDLQAYLS LNKLKSTFNK LQAPVINGEG SHNFKLEVED TVLTPRYLGA VIEDIKVEES PEWLKNRLKA IGLGPINNVV DITNYILHSY GQPLHAFDAA KINGSVVKVG VAPEGTKFKT LDGTERTLNG TEIMIKDGDN TPMCIAGVFG GENSGVSEGT TSVFLESAYF NPVAVRKAAK AHGLNTDASF RFERGVDPNI TKDALLKAIE MIQEIAGGKL KGEILESFPK KVDYFPVILR YSKVDQILGI KIHRETIKEI LKSLDILVLN EIKDGLEISV PPYRADVTRE IDVIEEILRV YGYNKVSSQD KIAFTPVRLT LDDQDSLENF WARMLQSNGF NEVMNNSLTS LKEEKADAVT LLNPLSNELS TMRQSLLEGL LQNADYNIKR KNQDIKFFEL GKIYFKKEKF EERKQLAILV SGNNNPENWM LSKSPTDFFI LKGYVQLLLD KLGLNTTEKA LEDARFSDAL EIVAEGKTIA RIGIVSKALL KEADLSQPAY YAEIELEACQ ALRSTTNFKF VDIPKFNKIR RDLALLVDKT VSYNDLLQAS NGISANLKKI QLFDVYEGKN LPEGKKSYAL SFELLNTEKT LEEAEIANIM NKLIKKYQKE FNAELRS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa tttctgataa gtggcttaga actcaccttg aaacagattt gtcagctgaa aagcttggaa taattcttac agatttaggt ctggaagtgg aaggaataga tccttatgaa tctgtgaaag gaagtcttaa tggagtagtg gttggaaaag tgcttacttg tgagcagcat cctaatgcag ataaactgaa agtaactact gtagacatta ataccggaac acctttacat atcgtttgtg gtgcaccgaa tgttgctgca gaacaaaagg tagctgttgc tacaataggt actacaattc atctgagtaa tggtgattcc tttaaaattg ctaaatccaa acttagagga gaggtatctg aaggaatgct ttgcgctgaa gacgaattag gtcttggaga aagccatgcc ggaattatgg tattgccgga agattatgaa attggaaaac ctttatccga ttatattaag gtagagcagg atgaggttta tgaaatcgga cttacaccca acagaacaga tgcgatgtcg cattatggtg ttgccagaga ccttcaggct tatttatctt taaataaatt aaaatctaca ttcaacaagc ttcaggctcc tgtaattaat ggagaaggaa gtcataactt taaacttgaa gttgaagata cagtgttaac acctcgttat ttaggcgctg ttatcgaaga tattaaagtt gaggaatctc cggagtggtt aaagaaccgt ctgaaagcta ttggccttgg accaattaat aatgttgtag atattaccaa ctatatttta cattcttatg ggcagccttt acatgctttt gatgctgcaa agatcaatgg aagtgttgta aaggtaggag tggctcctga aggaacgaaa ttcaaaactc tggatggtac agagcgtact ttaaatggta ctgaaattat gattaaagac ggagataata caccaatgtg tattgccgga gtctttggtg gtgaaaattc aggagtttca gaaggaacaa cttcagtgtt cttagaaagt gcatatttca accctgtagc agtacgtaaa gcagcgaagg cacatggtct aaatacagat gcttctttcc gtttcgaaag aggtgtagat cctaatatta ctaaagatgc tttattaaaa gcaatagaga tgattcagga gattgcaggt ggaaaactaa aaggagaaat ccttgaatct tttcctaaaa aagttgatta ttttcctgtt atccttaggt attccaaagt agaccagatt ctggggatta agattcacag agaaacaata aaagaaatcc tgaaatctct tgatatatta gttctgaatg agattaaaga cggattggaa atttctgttc cgccatacag agccgatgta accagagaaa ttgatgttat agaagaaatt cttcgtgttt acggatataa caaagtaagc tctcaggata aaatagcatt tacaccagtt cgtttaacat tagatgatca ggattctctg gaaaatttct gggcaagaat gttacagtct aatggcttca atgaagtaat gaataactct ctgacttctc ttaaagaaga gaaagcagat gctgttacct tactgaatcc tttgagtaat gaacttagca caatgcgcca gtctttatta gaaggattat tacagaatgc tgattacaat atcaagcgta agaatcagga tattaaattc tttgagcttg ggaaaatcta cttcaaaaaa gagaaatttg aagagcgcaa acaattggca attcttgttt ccggtaataa taatcctgaa aactggatgc tgagtaagtc tccaaccgac tttttcatcc tgaaaggtta tgttcagtta ttactggata aattaggact aaatacgact gaaaaagctt tagaagatgc acgcttctca gatgcattag aaattgttgc tgaaggaaaa actattgcca gaataggtat tgtaagcaag gctttattaa aagaggcaga tctttctcaa cctgcttatt atgcagaaat agaattagaa gcttgtcagg ctttaagaag cacgactaac tttaaattcg tagatattcc gaagtttaat aagataagaa gggatttagc gttattagta gataaaactg tttcttataa cgatttgctt caggcatcta acggtatttc agctaatctt aaaaagattc agttattcga tgtttacgaa ggtaaaaacc ttccggaagg taaaaaatct tatgccctaa gctttgaatt attaaatacc gagaagacat tagaagaagc agagattgca aatattatga ataagctgat caagaagtat cagaaagaat ttaatgctga actgcgttca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat