SSGCID: Target Page

ElanA.18889.a

Alanine--tRNA ligase

CENTER ID:	ElanA.18889.a
ORGANISM:	Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.18889.a.B1.GE42439	Full length(ElanA.18889.a)		1	867

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.18889.a.B1.PS38538	Full length(ElanA.18889.a)		1	867

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1338011.4.peg.2188
UniProt:	A0A077EE99

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSQEIRQRF LDFFKEKGHQ IVPSAPIVLK DDPTLMFSNS GMTQFKDFFL GYKEPSSLRI ADTQKCLRVS GKHNDLDDVG RDTYHHTMFE MLGNWSFGDY FKKEAIAWAW ELLTEVYKIS KDDLYVTIFE GDASENLERD QDAYNFWKEH IDESRIINGN KKDNFWEMGD TGPCGPCSEI HVDIRSAEEK AKVSGRELVN QDHPQVIEVW NLVFMEFLRK ADKSLEKLPK QNVDTGMGFE RLCMALQGKE SNYDTDVFTP LISKVEEISG KKYTGILENE KDIAIRVVVD HIRAVAFAIA DGQLPSNGGA GYVIRRILRR AISYAYRFLD MKEAFLYQLV VVLKDQMGSF FPEIVKQEKL VTEVIKEEEN SFLKTIEHGL VRLDAIIQAT INNNEKNLPG EQVFELYDTF GFPADLSRII AEEKGLSIDE AGFEEEMNKQ KQRSKQSSAS KTYDWVILEE KPENFVGYDL TENEVKITRY RKIENKDGEF YQIVLDETPF YAEGGGQVGD KGVLENAAES IEILDTKKEN NLNISLIATL PQDVKASFTA KVDLSKRKDT ENNHSATHLL HEALRNVLGT HVEQKGSFVG PDYLRFDFSH FSKMTEEEWA AVEQQVNEKI RANIALQEYR NIPIQEAMDK GAMALFGEKY GDNVRMIEFG TSKELCGGTH VKNTGEIGLF KIESEGSAAA GIRRIEAITG SKAREYFETL EKNYQEIAQF LKSKDVLKSV QKLVEENQSL KSEIESFKAE KAKQEALQWK NEYQDKGDKK LLVKKTSMDA GSIKDIVFQL KKEIPGSLTI ILSNAGDKPM ITIGVSDDLT QTYQAGALIK DLAKEIQGGG GGAPAFATAG GKNLDGLEKA FEKAQQL

NT Sequence

ATGACTTCAC AAGAGATACG CCAAAGGTTC TTAGATTTTT TTAAAGAAAA AGGACATCAG ATCGTTCCTT CTGCACCAAT TGTGCTAAAG GATGATCCTA CATTAATGTT TTCCAATTCG GGGATGACGC AATTCAAAGA TTTTTTCTTA GGCTACAAAG AGCCTTCAAG TCTTAGAATT GCCGATACAC AAAAATGTCT TCGTGTTTCC GGAAAACATA ATGACCTGGA TGATGTAGGC CGCGATACCT ACCACCACAC AATGTTCGAA ATGCTGGGCA ACTGGTCTTT CGGTGATTAT TTCAAAAAAG AAGCAATAGC CTGGGCGTGG GAACTTCTTA CCGAAGTATA TAAAATTTCC AAAGACGATT TGTATGTAAC TATTTTTGAA GGTGATGCTT CCGAAAATCT GGAAAGAGAT CAGGATGCTT ACAACTTCTG GAAAGAACAT ATAGATGAAT CCAGAATTAT TAATGGTAAC AAAAAAGATA ACTTCTGGGA AATGGGTGAT ACCGGACCAT GTGGACCATG TTCTGAAATT CACGTAGATA TTCGTAGTGC GGAAGAAAAA GCAAAAGTAT CGGGAAGAGA ATTAGTAAAT CAGGATCATC CTCAAGTAAT TGAAGTGTGG AATCTTGTAT TTATGGAATT CCTGAGAAAG GCTGACAAGT CTCTGGAGAA ATTACCGAAA CAGAATGTAG ACACCGGTAT GGGCTTCGAA CGTCTTTGTA TGGCTTTACA AGGAAAAGAG TCTAACTACG ATACAGATGT ATTCACACCA TTAATTTCCA AAGTAGAAGA AATTTCAGGT AAAAAATATA CAGGTATTCT GGAAAACGAA AAGGACATTG CCATTCGTGT AGTTGTAGAC CATATCCGTG CGGTAGCATT TGCTATAGCC GATGGACAAT TGCCATCCAA TGGTGGTGCC GGATATGTTA TCCGCAGAAT CCTAAGACGT GCTATTTCTT ATGCTTACAG ATTCCTGGAT ATGAAGGAAG CTTTCCTTTA TCAACTTGTA GTTGTACTAA AAGATCAGAT GGGATCTTTC TTCCCGGAAA TTGTAAAACA GGAGAAGTTA GTTACTGAAG TGATTAAGGA GGAGGAAAAT TCATTCCTGA AAACTATTGA ACACGGATTG GTAAGACTGG ATGCAATTAT CCAGGCTACC ATCAATAATA ACGAGAAAAA CCTCCCAGGT GAACAAGTAT TCGAATTATA TGATACTTTC GGATTCCCGG CTGACCTTTC CCGTATCATT GCTGAAGAAA AAGGCTTAAG TATAGATGAA GCCGGTTTTG AGGAGGAAAT GAACAAGCAA AAGCAACGTT CAAAACAATC TTCAGCTTCT AAAACTTATG ACTGGGTAAT ACTGGAAGAG AAACCTGAAA ATTTCGTAGG TTATGATCTT ACAGAAAATG AAGTAAAAAT AACCCGATAC AGAAAGATTG AGAATAAAGA TGGTGAGTTT TATCAAATCG TACTGGATGA GACTCCTTTC TATGCAGAAG GTGGTGGCCA GGTAGGTGAT AAAGGTGTGC TAGAAAATGC TGCCGAAAGC ATTGAAATTC TGGACACTAA AAAAGAGAAT AACCTTAACA TCTCTTTAAT TGCTACTCTT CCACAGGATG TAAAAGCAAG CTTTACAGCA AAGGTTGATC TGTCTAAAAG AAAGGATACG GAAAACAACC ACTCTGCTAC TCACCTTTTA CACGAAGCTT TAAGAAATGT ATTAGGAACT CACGTAGAAC AAAAGGGTTC TTTTGTAGGA CCAGATTATT TACGTTTCGA CTTTTCGCAT TTCAGCAAAA TGACAGAAGA GGAATGGGCT GCCGTAGAGC AACAGGTAAA TGAAAAAATC AGAGCTAATA TAGCACTTCA GGAATACAGA AACATTCCGA TTCAGGAAGC TATGGATAAA GGTGCTATGG CTTTATTTGG TGAGAAATAC GGTGACAATG TCCGCATGAT AGAATTTGGA ACATCTAAAG AATTATGTGG AGGAACACAC GTAAAGAACA CCGGAGAAAT AGGACTTTTC AAAATAGAAT CTGAAGGTTC GGCTGCGGCT GGAATCCGTA GAATTGAAGC AATCACCGGA TCTAAAGCAA GAGAATATTT CGAAACATTA GAAAAAAACT ACCAGGAAAT AGCACAGTTC CTGAAATCTA AAGATGTATT GAAGTCTGTT CAGAAACTGG TAGAAGAAAA CCAATCCTTA AAATCAGAAA TTGAAAGTTT TAAAGCTGAA AAAGCGAAAC AAGAAGCTTT ACAATGGAAA AATGAATACC AGGATAAAGG TGATAAAAAG CTATTGGTAA AGAAAACATC AATGGACGCA GGGTCTATTA AGGATATTGT GTTCCAGTTG AAAAAAGAGA TTCCGGGATC ATTAACGATT ATCTTATCTA ATGCTGGTGA CAAACCAATG ATTACAATCG GAGTTTCTGA TGACCTTACT CAGACTTACC AGGCCGGAGC TTTAATTAAA GATCTTGCTA AAGAGATACA AGGCGGCGGT GGCGGTGCTC CTGCATTTGC TACAGCCGGA GGTAAAAACC TTGACGGTTT AGAAAAAGCT TTTGAGAAAG CGCAACAGTT A

Details for ElanA.18889.a.B1.GE42439

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	99kDa
OBSERVED MW:	99kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT SQEIRQRFLD FFKEKGHQIV PSAPIVLKDD PTLMFSNSGM TQFKDFFLGY KEPSSLRIAD TQKCLRVSGK HNDLDDVGRD TYHHTMFEML GNWSFGDYFK KEAIAWAWEL LTEVYKISKD DLYVTIFEGD ASENLERDQD AYNFWKEHID ESRIINGNKK DNFWEMGDTG PCGPCSEIHV DIRSAEEKAK VSGRELVNQD HPQVIEVWNL VFMEFLRKAD KSLEKLPKQN VDTGMGFERL CMALQGKESN YDTDVFTPLI SKVEEISGKK YTGILENEKD IAIRVVVDHI RAVAFAIADG QLPSNGGAGY VIRRILRRAI SYAYRFLDMK EAFLYQLVVV LKDQMGSFFP EIVKQEKLVT EVIKEEENSF LKTIEHGLVR LDAIIQATIN NNEKNLPGEQ VFELYDTFGF PADLSRIIAE EKGLSIDEAG FEEEMNKQKQ RSKQSSASKT YDWVILEEKP ENFVGYDLTE NEVKITRYRK IENKDGEFYQ IVLDETPFYA EGGGQVGDKG VLENAAESIE ILDTKKENNL NISLIATLPQ DVKASFTAKV DLSKRKDTEN NHSATHLLHE ALRNVLGTHV EQKGSFVGPD YLRFDFSHFS KMTEEEWAAV EQQVNEKIRA NIALQEYRNI PIQEAMDKGA MALFGEKYGD NVRMIEFGTS KELCGGTHVK NTGEIGLFKI ESEGSAAAGI RRIEAITGSK AREYFETLEK NYQEIAQFLK SKDVLKSVQK LVEENQSLKS EIESFKAEKA KQEALQWKNE YQDKGDKKLL VKKTSMDAGS IKDIVFQLKK EIPGSLTIIL SNAGDKPMIT IGVSDDLTQT YQAGALIKDL AKEIQGGGGG APAFATAGGK NLDGLEKAFE KAQQL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactt cacaagagat acgccaaagg ttcttagatt tttttaaaga aaaaggacat cagatcgttc cttctgcacc aattgtgcta aaggatgatc ctacattaat gttttccaat tcggggatga cgcaattcaa agattttttc ttaggctaca aagagccttc aagtcttaga attgccgata cacaaaaatg tcttcgtgtt tccggaaaac ataatgacct ggatgatgta ggccgcgata cctaccacca cacaatgttc gaaatgctgg gcaactggtc tttcggtgat tatttcaaaa aagaagcaat agcctgggcg tgggaacttc ttaccgaagt atataaaatt tccaaagacg atttgtatgt aactattttt gaaggtgatg cttccgaaaa tctggaaaga gatcaggatg cttacaactt ctggaaagaa catatagatg aatccagaat tattaatggt aacaaaaaag ataacttctg ggaaatgggt gataccggac catgtggacc atgttctgaa attcacgtag atattcgtag tgcggaagaa aaagcaaaag tatcgggaag agaattagta aatcaggatc atcctcaagt aattgaagtg tggaatcttg tatttatgga attcctgaga aaggctgaca agtctctgga gaaattaccg aaacagaatg tagacaccgg tatgggcttc gaacgtcttt gtatggcttt acaaggaaaa gagtctaact acgatacaga tgtattcaca ccattaattt ccaaagtaga agaaatttca ggtaaaaaat atacaggtat tctggaaaac gaaaaggaca ttgccattcg tgtagttgta gaccatatcc gtgcggtagc atttgctata gccgatggac aattgccatc caatggtggt gccggatatg ttatccgcag aatcctaaga cgtgctattt cttatgctta cagattcctg gatatgaagg aagctttcct ttatcaactt gtagttgtac taaaagatca gatgggatct ttcttcccgg aaattgtaaa acaggagaag ttagttactg aagtgattaa ggaggaggaa aattcattcc tgaaaactat tgaacacgga ttggtaagac tggatgcaat tatccaggct accatcaata ataacgagaa aaacctccca ggtgaacaag tattcgaatt atatgatact ttcggattcc cggctgacct ttcccgtatc attgctgaag aaaaaggctt aagtatagat gaagccggtt ttgaggagga aatgaacaag caaaagcaac gttcaaaaca atcttcagct tctaaaactt atgactgggt aatactggaa gagaaacctg aaaatttcgt aggttatgat cttacagaaa atgaagtaaa aataacccga tacagaaaga ttgagaataa agatggtgag ttttatcaaa tcgtactgga tgagactcct ttctatgcag aaggtggtgg ccaggtaggt gataaaggtg tgctagaaaa tgctgccgaa agcattgaaa ttctggacac taaaaaagag aataacctta acatctcttt aattgctact cttccacagg atgtaaaagc aagctttaca gcaaaggttg atctgtctaa aagaaaggat acggaaaaca accactctgc tactcacctt ttacacgaag ctttaagaaa tgtattagga actcacgtag aacaaaaggg ttcttttgta ggaccagatt atttacgttt cgacttttcg catttcagca aaatgacaga agaggaatgg gctgccgtag agcaacaggt aaatgaaaaa atcagagcta atatagcact tcaggaatac agaaacattc cgattcagga agctatggat aaaggtgcta tggctttatt tggtgagaaa tacggtgaca atgtccgcat gatagaattt ggaacatcta aagaattatg tggaggaaca cacgtaaaga acaccggaga aataggactt ttcaaaatag aatctgaagg ttcggctgcg gctggaatcc gtagaattga agcaatcacc ggatctaaag caagagaata tttcgaaaca ttagaaaaaa actaccagga aatagcacag ttcctgaaat ctaaagatgt attgaagtct gttcagaaac tggtagaaga aaaccaatcc ttaaaatcag aaattgaaag ttttaaagct gaaaaagcga aacaagaagc tttacaatgg aaaaatgaat accaggataa aggtgataaa aagctattgg taaagaaaac atcaatggac gcagggtcta ttaaggatat tgtgttccag ttgaaaaaag agattccggg atcattaacg attatcttat ctaatgctgg tgacaaacca atgattacaa tcggagtttc tgatgacctt actcagactt accaggccgg agctttaatt aaagatcttg ctaaagagat acaaggcggc ggtggcggtg ctcctgcatt tgctacagcc ggaggtaaaa accttgacgg tttagaaaaa gcttttgaga aagcgcaaca gttatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for ElanA.18889.a.B1.PS38538

PURIFICATION DATe:	12/4/2018
CONCENTRATION:	12.15mg/ml
OBSERVED MW:	100kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTT CACAAGAGAT ACGCCAAAGG TTCTTAGATT TTTTTAAAGA AAAAGGACAT CAGATCGTTC CTTCTGCACC AATTGTGCTA AAGGATGATC CTACATTAAT GTTTTCCAAT TCGGGGATGA CGCAATTCAA AGATTTTTTC TTAGGCTACA AAGAGCCTTC AAGTCTTAGA ATTGCCGATA CACAAAAATG TCTTCGTGTT TCCGGAAAAC ATAATGACCT GGATGATGTA GGCCGCGATA CCTACCACCA CACAATGTTC GAAATGCTGG GCAACTGGTC TTTCGGTGAT TATTTCAAAA AAGAAGCAAT AGCCTGGGCG TGGGAACTTC TTACCGAAGT ATATAAAATT TCCAAAGACG ATTTGTATGT AACTATTTTT GAAGGTGATG CTTCCGAAAA TCTGGAAAGA GATCAGGATG CTTACAACTT CTGGAAAGAA CATATAGATG AATCCAGAAT TATTAATGGT AACAAAAAAG ATAACTTCTG GGAAATGGGT GATACCGGAC CATGTGGACC ATGTTCTGAA ATTCACGTAG ATATTCGTAG TGCGGAAGAA AAAGCAAAAG TATCGGGAAG AGAATTAGTA AATCAGGATC ATCCTCAAGT AATTGAAGTG TGGAATCTTG TATTTATGGA ATTCCTGAGA AAGGCTGACA AGTCTCTGGA GAAATTACCG AAACAGAATG TAGACACCGG TATGGGCTTC GAACGTCTTT GTATGGCTTT ACAAGGAAAA GAGTCTAACT ACGATACAGA TGTATTCACA CCATTAATTT CCAAAGTAGA AGAAATTTCA GGTAAAAAAT ATACAGGTAT TCTGGAAAAC GAAAAGGACA TTGCCATTCG TGTAGTTGTA GACCATATCC GTGCGGTAGC ATTTGCTATA GCCGATGGAC AATTGCCATC CAATGGTGGT GCCGGATATG TTATCCGCAG AATCCTAAGA CGTGCTATTT CTTATGCTTA CAGATTCCTG GATATGAAGG AAGCTTTCCT TTATCAACTT GTAGTTGTAC TAAAAGATCA GATGGGATCT TTCTTCCCGG AAATTGTAAA ACAGGAGAAG TTAGTTACTG AAGTGATTAA GGAGGAGGAA AATTCATTCC TGAAAACTAT TGAACACGGA TTGGTAAGAC TGGATGCAAT TATCCAGGCT ACCATCAATA ATAACGAGAA AAACCTCCCA GGTGAACAAG TATTCGAATT ATATGATACT TTCGGATTCC CGGCTGACCT TTCCCGTATC ATTGCTGAAG AAAAAGGCTT AAGTATAGAT GAAGCCGGTT TTGAGGAGGA AATGAACAAG CAAAAGCAAC GTTCAAAACA ATCTTCAGCT TCTAAAACTT ATGACTGGGT AATACTGGAA GAGAAACCTG AAAATTTCGT AGGTTATGAT CTTACAGAAA ATGAAGTAAA AATAACCCGA TACAGAAAGA TTGAGAATAA AGATGGTGAG TTTTATCAAA TCGTACTGGA TGAGACTCCT TTCTATGCAG AAGGTGGTGG CCAGGTAGGT GATAAAGGTG TGCTAGAAAA TGCTGCCGAA AGCATTGAAA TTCTGGACAC TAAAAAAGAG AATAACCTTA ACATCTCTTT AATTGCTACT CTTCCACAGG ATGTAAAAGC AAGCTTTACA GCAAAGGTTG ATCTGTCTAA AAGAAAGGAT ACGGAAAACA ACCACTCTGC TACTCACCTT TTACACGAAG CTTTAAGAAA TGTATTAGGA ACTCACGTAG AACAAAAGGG TTCTTTTGTA GGACCAGATT ATTTACGTTT CGACTTTTCG CATTTCAGCA AAATGACAGA AGAGGAATGG GCTGCCGTAG AGCAACAGGT AAATGAAAAA ATCAGAGCTA ATATAGCACT TCAGGAATAC AGAAACATTC CGATTCAGGA AGCTATGGAT AAAGGTGCTA TGGCTTTATT TGGTGAGAAA TACGGTGACA ATGTCCGCAT GATAGAATTT GGAACATCTA AAGAATTATG TGGAGGAACA CACGTAAAGA ACACCGGAGA AATAGGACTT TTCAAAATAG AATCTGAAGG TTCGGCTGCG GCTGGAATCC GTAGAATTGA AGCAATCACC GGATCTAAAG CAAGAGAATA TTTCGAAACA TTAGAAAAAA ACTACCAGGA AATAGCACAG TTCCTGAAAT CTAAAGATGT ATTGAAGTCT GTTCAGAAAC TGGTAGAAGA AAACCAATCC TTAAAATCAG AAATTGAAAG TTTTAAAGCT GAAAAAGCGA AACAAGAAGC TTTACAATGG AAAAATGAAT ACCAGGATAA AGGTGATAAA AAGCTATTGG TAAAGAAAAC ATCAATGGAC GCAGGGTCTA TTAAGGATAT TGTGTTCCAG TTGAAAAAAG AGATTCCGGG ATCATTAACG ATTATCTTAT CTAATGCTGG TGACAAACCA ATGATTACAA TCGGAGTTTC TGATGACCTT ACTCAGACTT ACCAGGCCGG AGCTTTAATT AAAGATCTTG CTAAAGAGAT ACAAGGCGGC GGTGGCGGTG CTCCTGCATT TGCTACAGCC GGAGGTAAAA ACCTTGACGG TTTAGAAAAA GCTTTTGAGA AAGCGCAACA GTTATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT