SSGCID: Target Page

ElanA.18849.a

Valine--tRNA ligase

CENTER ID:	ElanA.18849.a
ORGANISM:	Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.18849.a.B1.GE42438	Full length(ElanA.18849.a)		1	871

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.18849.a.B1.PS38552	Full length(ElanA.18849.a)		1	871

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1338011.4.peg.3415
UniProt:	A0A077ELP4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEISDKYNPQ QAEQKWYQYW QENKFFHSEP DEREPYTVVI PPPNVTGILH MGHMLNNTLQ DVLVRRARMR GYNACWVPGT DHASIATEAK VVAKLKAEGI SKQDIGREKF LEHAWDWTHQ YGGTILEQLK KLGCSCDWDR TRFTMEDDLS KAVIKVFVDL YNKGLVYRGY KMVNWDPEAK TNISDEEVIY KEQNGKLYFL KYQIVGSDEF LTVATTRPET IFGDTAVCIN PNDERYTHLK GKKVIVPIVG REVPIIEDDY VDIEFGTGAL KITPAHDIND YEIGQRHQLE IIDSMDDNAV LNENGKHYQG KDRFTVRKEI AKELEEKGLL LKSEDYLNKV GTSERTGAVI EPKISVQWFL KMSELAKPAL DVVMDDEVKF YPEKFKNTYR HWMENVHDWN ISRQLWWGHR IPAYYYASGE NDFVVAETIE AALELAKQKT GNAELTTDNL RQDEDALDTW FSAWLWPMSV FDGITAPDNK DINYYYPTSD LVTGPDIIFF WVARMIMAGL EFKNQVPFKN VYFTGIVRDK QRRKMSKSLG NSPDPIELMD KYGSDGVRVG ILLSSAAGND LMFDEDLMLQ GRNFATKIWN AFKLTQSWKK EDKEISEENA QAIEWFEAEF NKTVAAINDQ FEKFRISDAL HLLYKLVWDD FCSWYLEIVK PNFGEAISQQ VYDKTIEFFG NLMKFLHPFM PFLTEEIWQA IAERQTSEAL IITQQNKETA FNADILKNFE QTKDIVSGVR NYRQSKGISP REEVEVYTNV SQFENEAVIK KLANVSEIHY AQKTDKPSFT FLVGAVECSI PLSENLDLGE EKTKTEEELK YLKGFLISVE KKLSNEKFMA GAPQQVVDNE LKKQKDAQDK ITLLEEKLKT L

NT Sequence

ATGGAAATTT CCGACAAGTA CAATCCGCAA CAAGCAGAAC AAAAGTGGTA CCAATATTGG CAGGAAAATA AATTTTTTCA TTCCGAGCCA GATGAAAGAG AGCCATATAC TGTCGTCATA CCACCTCCAA ATGTGACAGG GATTTTGCAC ATGGGGCATA TGCTTAATAA TACATTGCAG GATGTACTGG TTCGCCGTGC ACGTATGCGT GGTTATAATG CATGTTGGGT TCCCGGGACC GATCATGCCT CTATTGCTAC AGAAGCTAAA GTAGTAGCTA AATTGAAAGC TGAAGGAATT TCTAAGCAGG ATATCGGACG CGAGAAGTTT TTAGAACATG CATGGGACTG GACGCACCAA TATGGTGGAA CAATTCTGGA GCAGCTAAAA AAATTAGGAT GCTCTTGTGA TTGGGACAGA ACCCGTTTCA CGATGGAAGA TGATCTGTCT AAAGCGGTTA TCAAAGTATT CGTGGACCTT TATAATAAAG GACTTGTTTA CCGTGGTTAT AAAATGGTAA ACTGGGACCC GGAGGCAAAG ACCAATATCT CAGACGAGGA GGTAATCTAT AAAGAGCAGA ACGGTAAATT ATATTTCCTT AAATATCAGA TCGTAGGTTC AGATGAGTTC CTGACTGTTG CTACAACACG TCCGGAAACA ATATTCGGAG ATACTGCTGT ATGTATCAAT CCTAACGATG AACGTTACAC ACACCTGAAA GGTAAAAAAG TAATCGTGCC AATTGTAGGG CGAGAAGTTC CTATTATTGA AGACGATTAT GTTGATATAG AATTTGGTAC CGGAGCATTG AAGATTACTC CTGCTCATGA TATCAATGAC TACGAAATTG GTCAACGTCA CCAGTTAGAG ATTATAGACT CTATGGACGA TAATGCTGTT CTTAATGAAA ATGGAAAGCA CTATCAAGGC AAAGACAGAT TTACAGTAAG AAAAGAAATT GCTAAAGAAC TTGAAGAGAA AGGTCTTTTG TTAAAATCTG AAGACTATCT GAATAAAGTA GGTACATCCG AAAGAACAGG GGCTGTGATA GAGCCGAAAA TCTCTGTACA GTGGTTCCTG AAGATGAGTG AGCTGGCAAA GCCTGCATTG GATGTTGTAA TGGATGATGA AGTTAAATTC TATCCGGAAA AATTCAAGAA TACATACAGA CACTGGATGG AGAACGTACA CGACTGGAAT ATATCTCGCC AGCTATGGTG GGGGCACAGA ATTCCGGCCT ATTACTATGC ATCTGGTGAG AACGATTTTG TAGTTGCTGA AACGATTGAA GCAGCATTAG AGCTTGCAAA ACAAAAAACG GGTAATGCAG AACTAACAAC TGATAACCTA AGACAGGATG AAGATGCATT AGATACATGG TTCTCTGCAT GGTTATGGCC AATGTCAGTA TTCGATGGTA TTACGGCACC AGATAATAAA GATATCAATT ACTATTATCC AACTTCGGAT TTGGTAACTG GACCAGATAT TATCTTCTTC TGGGTAGCGC GTATGATTAT GGCTGGATTA GAATTCAAAA ACCAGGTTCC GTTTAAAAAT GTATATTTTA CCGGAATTGT AAGAGATAAA CAGCGTCGTA AGATGTCTAA ATCTTTAGGA AACTCTCCGG ACCCGATTGA ATTAATGGAC AAATATGGCT CCGACGGAGT AAGAGTAGGG ATTTTGTTAA GCTCAGCGGC AGGTAATGAT CTTATGTTCG ATGAAGATTT AATGCTTCAG GGACGTAATT TCGCAACCAA AATATGGAAT GCATTCAAGC TGACACAAAG TTGGAAGAAA GAGGATAAAG AGATTTCTGA AGAAAACGCA CAAGCTATTG AATGGTTTGA AGCAGAATTT AATAAGACTG TGGCTGCAAT TAATGACCAG TTTGAAAAGT TCAGAATTTC TGACGCATTA CATTTGTTGT ATAAATTAGT TTGGGACGAT TTCTGTTCAT GGTATCTGGA AATTGTAAAA CCAAACTTTG GCGAGGCTAT CAGTCAGCAG GTATATGACA AAACAATAGA ATTCTTCGGA AATCTAATGA AGTTCTTGCA TCCTTTCATG CCATTCCTGA CAGAAGAAAT TTGGCAGGCG ATTGCAGAGA GACAAACTTC TGAAGCGTTA ATTATTACCC AACAAAATAA GGAAACAGCT TTTAACGCTG ATATTCTTAA AAACTTCGAG CAAACGAAGG ACATCGTTTC AGGAGTAAGA AACTACCGCC AGAGCAAAGG TATTTCTCCA AGAGAAGAAG TTGAGGTTTA TACTAATGTT TCTCAGTTCG AAAATGAAGC TGTCATCAAA AAATTGGCTA ATGTTTCGGA GATCCATTAT GCACAAAAAA CGGATAAACC AAGCTTCACA TTCCTTGTAG GTGCAGTGGA ATGCTCTATT CCATTGAGTG AGAATCTTGA TTTGGGTGAG GAGAAAACCA AAACAGAAGA AGAACTGAAG TATCTGAAAG GTTTCCTTAT CTCTGTTGAA AAGAAGCTTA GTAATGAAAA ATTCATGGCT GGAGCTCCAC AACAAGTTGT AGATAATGAG CTGAAAAAAC AAAAAGATGC TCAGGATAAA ATAACATTAT TGGAAGAGAA ACTGAAAACT TTA

Details for ElanA.18849.a.B1.GE42438

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	102kDa
OBSERVED MW:	102kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHME ISDKYNPQQA EQKWYQYWQE NKFFHSEPDE REPYTVVIPP PNVTGILHMG HMLNNTLQDV LVRRARMRGY NACWVPGTDH ASIATEAKVV AKLKAEGISK QDIGREKFLE HAWDWTHQYG GTILEQLKKL GCSCDWDRTR FTMEDDLSKA VIKVFVDLYN KGLVYRGYKM VNWDPEAKTN ISDEEVIYKE QNGKLYFLKY QIVGSDEFLT VATTRPETIF GDTAVCINPN DERYTHLKGK KVIVPIVGRE VPIIEDDYVD IEFGTGALKI TPAHDINDYE IGQRHQLEII DSMDDNAVLN ENGKHYQGKD RFTVRKEIAK ELEEKGLLLK SEDYLNKVGT SERTGAVIEP KISVQWFLKM SELAKPALDV VMDDEVKFYP EKFKNTYRHW MENVHDWNIS RQLWWGHRIP AYYYASGEND FVVAETIEAA LELAKQKTGN AELTTDNLRQ DEDALDTWFS AWLWPMSVFD GITAPDNKDI NYYYPTSDLV TGPDIIFFWV ARMIMAGLEF KNQVPFKNVY FTGIVRDKQR RKMSKSLGNS PDPIELMDKY GSDGVRVGIL LSSAAGNDLM FDEDLMLQGR NFATKIWNAF KLTQSWKKED KEISEENAQA IEWFEAEFNK TVAAINDQFE KFRISDALHL LYKLVWDDFC SWYLEIVKPN FGEAISQQVY DKTIEFFGNL MKFLHPFMPF LTEEIWQAIA ERQTSEALII TQQNKETAFN ADILKNFEQT KDIVSGVRNY RQSKGISPRE EVEVYTNVSQ FENEAVIKKL ANVSEIHYAQ KTDKPSFTFL VGAVECSIPL SENLDLGEEK TKTEEELKYL KGFLISVEKK LSNEKFMAGA PQQVVDNELK KQKDAQDKIT LLEEKLKTL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa tttccgacaa gtacaatccg caacaagcag aacaaaagtg gtaccaatat tggcaggaaa ataaattttt tcattccgag ccagatgaaa gagagccata tactgtcgtc ataccacctc caaatgtgac agggattttg cacatggggc atatgcttaa taatacattg caggatgtac tggttcgccg tgcacgtatg cgtggttata atgcatgttg ggttcccggg accgatcatg cctctattgc tacagaagct aaagtagtag ctaaattgaa agctgaagga atttctaagc aggatatcgg acgcgagaag tttttagaac atgcatggga ctggacgcac caatatggtg gaacaattct ggagcagcta aaaaaattag gatgctcttg tgattgggac agaacccgtt tcacgatgga agatgatctg tctaaagcgg ttatcaaagt attcgtggac ctttataata aaggacttgt ttaccgtggt tataaaatgg taaactggga cccggaggca aagaccaata tctcagacga ggaggtaatc tataaagagc agaacggtaa attatatttc cttaaatatc agatcgtagg ttcagatgag ttcctgactg ttgctacaac acgtccggaa acaatattcg gagatactgc tgtatgtatc aatcctaacg atgaacgtta cacacacctg aaaggtaaaa aagtaatcgt gccaattgta gggcgagaag ttcctattat tgaagacgat tatgttgata tagaatttgg taccggagca ttgaagatta ctcctgctca tgatatcaat gactacgaaa ttggtcaacg tcaccagtta gagattatag actctatgga cgataatgct gttcttaatg aaaatggaaa gcactatcaa ggcaaagaca gatttacagt aagaaaagaa attgctaaag aacttgaaga gaaaggtctt ttgttaaaat ctgaagacta tctgaataaa gtaggtacat ccgaaagaac aggggctgtg atagagccga aaatctctgt acagtggttc ctgaagatga gtgagctggc aaagcctgca ttggatgttg taatggatga tgaagttaaa ttctatccgg aaaaattcaa gaatacatac agacactgga tggagaacgt acacgactgg aatatatctc gccagctatg gtgggggcac agaattccgg cctattacta tgcatctggt gagaacgatt ttgtagttgc tgaaacgatt gaagcagcat tagagcttgc aaaacaaaaa acgggtaatg cagaactaac aactgataac ctaagacagg atgaagatgc attagataca tggttctctg catggttatg gccaatgtca gtattcgatg gtattacggc accagataat aaagatatca attactatta tccaacttcg gatttggtaa ctggaccaga tattatcttc ttctgggtag cgcgtatgat tatggctgga ttagaattca aaaaccaggt tccgtttaaa aatgtatatt ttaccggaat tgtaagagat aaacagcgtc gtaagatgtc taaatcttta ggaaactctc cggacccgat tgaattaatg gacaaatatg gctccgacgg agtaagagta gggattttgt taagctcagc ggcaggtaat gatcttatgt tcgatgaaga tttaatgctt cagggacgta atttcgcaac caaaatatgg aatgcattca agctgacaca aagttggaag aaagaggata aagagatttc tgaagaaaac gcacaagcta ttgaatggtt tgaagcagaa tttaataaga ctgtggctgc aattaatgac cagtttgaaa agttcagaat ttctgacgca ttacatttgt tgtataaatt agtttgggac gatttctgtt catggtatct ggaaattgta aaaccaaact ttggcgaggc tatcagtcag caggtatatg acaaaacaat agaattcttc ggaaatctaa tgaagttctt gcatcctttc atgccattcc tgacagaaga aatttggcag gcgattgcag agagacaaac ttctgaagcg ttaattatta cccaacaaaa taaggaaaca gcttttaacg ctgatattct taaaaacttc gagcaaacga aggacatcgt ttcaggagta agaaactacc gccagagcaa aggtatttct ccaagagaag aagttgaggt ttatactaat gtttctcagt tcgaaaatga agctgtcatc aaaaaattgg ctaatgtttc ggagatccat tatgcacaaa aaacggataa accaagcttc acattccttg taggtgcagt ggaatgctct attccattga gtgagaatct tgatttgggt gaggagaaaa ccaaaacaga agaagaactg aagtatctga aaggtttcct tatctctgtt gaaaagaagc ttagtaatga aaaattcatg gctggagctc cacaacaagt tgtagataat gagctgaaaa aacaaaaaga tgctcaggat aaaataacat tattggaaga gaaactgaaa actttatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for ElanA.18849.a.B1.PS38552

PURIFICATION DATe:	1/8/2019
CONCENTRATION:	18.44mg/ml
OBSERVED MW:	100kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAA TTTCCGACAA GTACAATCCG CAACAAGCAG AACAAAAGTG GTACCAATAT TGGCAGGAAA ATAAATTTTT TCATTCCGAG CCAGATGAAA GAGAGCCATA TACTGTCGTC ATACCACCTC CAAATGTGAC AGGGATTTTG CACATGGGGC ATATGCTTAA TAATACATTG CAGGATGTAC TGGTTCGCCG TGCACGTATG CGTGGTTATA ATGCATGTTG GGTTCCCGGG ACCGATCATG CCTCTATTGC TACAGAAGCT AAAGTAGTAG CTAAATTGAA AGCTGAAGGA ATTTCTAAGC AGGATATCGG ACGCGAGAAG TTTTTAGAAC ATGCATGGGA CTGGACGCAC CAATATGGTG GAACAATTCT GGAGCAGCTA AAAAAATTAG GATGCTCTTG TGATTGGGAC AGAACCCGTT TCACGATGGA AGATGATCTG TCTAAAGCGG TTATCAAAGT ATTCGTGGAC CTTTATAATA AAGGACTTGT TTACCGTGGT TATAAAATGG TAAACTGGGA CCCGGAGGCA AAGACCAATA TCTCAGACGA GGAGGTAATC TATAAAGAGC AGAACGGTAA ATTATATTTC CTTAAATATC AGATCGTAGG TTCAGATGAG TTCCTGACTG TTGCTACAAC ACGTCCGGAA ACAATATTCG GAGATACTGC TGTATGTATC AATCCTAACG ATGAACGTTA CACACACCTG AAAGGTAAAA AAGTAATCGT GCCAATTGTA GGGCGAGAAG TTCCTATTAT TGAAGACGAT TATGTTGATA TAGAATTTGG TACCGGAGCA TTGAAGATTA CTCCTGCTCA TGATATCAAT GACTACGAAA TTGGTCAACG TCACCAGTTA GAGATTATAG ACTCTATGGA CGATAATGCT GTTCTTAATG AAAATGGAAA GCACTATCAA GGCAAAGACA GATTTACAGT AAGAAAAGAA ATTGCTAAAG AACTTGAAGA GAAAGGTCTT TTGTTAAAAT CTGAAGACTA TCTGAATAAA GTAGGTACAT CCGAAAGAAC AGGGGCTGTG ATAGAGCCGA AAATCTCTGT ACAGTGGTTC CTGAAGATGA GTGAGCTGGC AAAGCCTGCA TTGGATGTTG TAATGGATGA TGAAGTTAAA TTCTATCCGG AAAAATTCAA GAATACATAC AGACACTGGA TGGAGAACGT ACACGACTGG AATATATCTC GCCAGCTATG GTGGGGGCAC AGAATTCCGG CCTATTACTA TGCATCTGGT GAGAACGATT TTGTAGTTGC TGAAACGATT GAAGCAGCAT TAGAGCTTGC AAAACAAAAA ACGGGTAATG CAGAACTAAC AACTGATAAC CTAAGACAGG ATGAAGATGC ATTAGATACA TGGTTCTCTG CATGGTTATG GCCAATGTCA GTATTCGATG GTATTACGGC ACCAGATAAT AAAGATATCA ATTACTATTA TCCAACTTCG GATTTGGTAA CTGGACCAGA TATTATCTTC TTCTGGGTAG CGCGTATGAT TATGGCTGGA TTAGAATTCA AAAACCAGGT TCCGTTTAAA AATGTATATT TTACCGGAAT TGTAAGAGAT AAACAGCGTC GTAAGATGTC TAAATCTTTA GGAAACTCTC CGGACCCGAT TGAATTAATG GACAAATATG GCTCCGACGG AGTAAGAGTA GGGATTTTGT TAAGCTCAGC GGCAGGTAAT GATCTTATGT TCGATGAAGA TTTAATGCTT CAGGGACGTA ATTTCGCAAC CAAAATATGG AATGCATTCA AGCTGACACA AAGTTGGAAG AAAGAGGATA AAGAGATTTC TGAAGAAAAC GCACAAGCTA TTGAATGGTT TGAAGCAGAA TTTAATAAGA CTGTGGCTGC AATTAATGAC CAGTTTGAAA AGTTCAGAAT TTCTGACGCA TTACATTTGT TGTATAAATT AGTTTGGGAC GATTTCTGTT CATGGTATCT GGAAATTGTA AAACCAAACT TTGGCGAGGC TATCAGTCAG CAGGTATATG ACAAAACAAT AGAATTCTTC GGAAATCTAA TGAAGTTCTT GCATCCTTTC ATGCCATTCC TGACAGAAGA AATTTGGCAG GCGATTGCAG AGAGACAAAC TTCTGAAGCG TTAATTATTA CCCAACAAAA TAAGGAAACA GCTTTTAACG CTGATATTCT TAAAAACTTC GAGCAAACGA AGGACATCGT TTCAGGAGTA AGAAACTACC GCCAGAGCAA AGGTATTTCT CCAAGAGAAG AAGTTGAGGT TTATACTAAT GTTTCTCAGT TCGAAAATGA AGCTGTCATC AAAAAATTGG CTAATGTTTC GGAGATCCAT TATGCACAAA AAACGGATAA ACCAAGCTTC ACATTCCTTG TAGGTGCAGT GGAATGCTCT ATTCCATTGA GTGAGAATCT TGATTTGGGT GAGGAGAAAA CCAAAACAGA AGAAGAACTG AAGTATCTGA AAGGTTTCCT TATCTCTGTT GAAAAGAAGC TTAGTAATGA AAAATTCATG GCTGGAGCTC CACAACAAGT TGTAGATAAT GAGCTGAAAA AACAAAAAGA TGCTCAGGAT AAAATAACAT TATTGGAAGA GAAACTGAAA ACTTTATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T