SSGCID: Target Page

ElanA.18755.a

Glycine--tRNA ligase

CENTER ID:	ElanA.18755.a
ORGANISM:	Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.18755.a.B1.GE41096	Full length(ElanA.18755.a)		1	513

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.18755.a.B1.PW38249	Full length(ElanA.18755.a)		1	513

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1338011.4.peg.250
UniProt:	A0A077EJT4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKQEDVFKK VISHAKEYGF IFPSSEIYDG LSAIYDYGQN GAELKNNIKQ YWWKAMVQLN ENIVGIDSAI FMHPTIWKAS GHVDAFNDPL IDNKDSKKRF RADVLVEDYC AKLEDKAQKE IDKAAKRFGE AFDKAQFEAT NPRVIEYREK QKTILSRLAK SLENEDLADV KSLIEELEIA DPDTGSKNWT EVRQFNLMFG TKLGATTETA MDLYLRPETA QGIFVNYLNV QKTSRHKLPF GIAQIGKAFR NEIVARQFIF RMREFEQMEM QFFVPPGTEL EYYEKWKQTR LKWHLALGLG EENYKFHDHE KLAHYANAAA DIEFKFPFGF KELEGIHSRT DFDLSAHEKF SGKKLQYFDP ERNESYVPYV VETSVGLDRL FLSILSHCLK DEVLEDGSER TVLSLPPALA PVKAAILPLM KKDGLGEYAE KIFNDLKFDF NLIYEDKDSI GKRYRRNDAL GTPFCITIDH DSLNDHTVTL RDRDTMQQER VPVSELRRII DDKVNFRNLL SKI

NT Sequence

ATGGCTAAAC AGGAAGACGT TTTCAAGAAG GTGATTTCTC ACGCGAAAGA GTATGGTTTT ATATTTCCGT CAAGTGAAAT TTATGACGGG CTTTCTGCAA TCTATGACTA CGGACAGAAC GGTGCTGAGC TGAAAAACAA TATCAAGCAG TATTGGTGGA AAGCAATGGT ACAACTGAAC GAAAATATTG TGGGAATCGA TTCAGCAATC TTTATGCACC CTACAATCTG GAAGGCTTCC GGACACGTGG ACGCTTTCAA TGATCCATTA ATTGATAATA AAGATTCTAA AAAACGTTTC CGTGCAGATG TTCTGGTAGA AGACTATTGT GCGAAATTAG AAGATAAAGC TCAGAAAGAG ATTGATAAAG CAGCAAAACG TTTTGGAGAA GCTTTTGATA AAGCTCAGTT TGAAGCTACA AACCCAAGAG TAATAGAATA CAGAGAGAAG CAGAAAACAA TTCTGTCTCG TTTGGCAAAA TCTCTTGAAA ATGAAGATCT GGCAGATGTA AAATCTCTGA TTGAAGAATT GGAAATTGCA GATCCGGATA CAGGTTCTAA AAACTGGACA GAAGTAAGAC AATTTAACCT GATGTTCGGT ACTAAGCTTG GTGCTACTAC AGAAACGGCA ATGGATCTTT ATCTTCGTCC GGAAACAGCA CAAGGTATTT TTGTAAATTA CCTGAACGTT CAGAAAACAT CTCGTCATAA GCTGCCTTTT GGTATTGCCC AAATAGGTAA AGCATTCCGT AACGAAATTG TTGCCAGACA ATTTATCTTC AGAATGCGTG AATTCGAACA AATGGAAATG CAGTTCTTCG TACCTCCTGG AACAGAGCTT GAATACTATG AAAAATGGAA ACAAACCCGT TTGAAATGGC ACCTGGCTTT AGGCCTTGGT GAAGAGAACT ACAAATTCCA CGATCATGAG AAACTAGCGC ATTATGCAAA TGCTGCGGCT GATATAGAAT TTAAATTCCC ATTTGGATTC AAAGAATTGG AAGGTATTCA CAGCAGAACT GATTTTGACC TTTCTGCACA TGAGAAATTC TCTGGTAAAA AACTACAATA TTTTGATCCG GAAAGAAATG AAAGCTATGT GCCATACGTA GTGGAAACTT CTGTAGGTTT GGACAGATTA TTCCTTTCTA TCCTTTCTCA TTGCCTGAAA GATGAAGTAC TAGAGGATGG ATCTGAAAGA ACTGTTCTTT CTCTTCCTCC GGCTTTAGCA CCAGTAAAAG CTGCTATTCT TCCGTTAATG AAAAAAGACG GTTTAGGTGA GTATGCTGAA AAAATCTTTA ACGATTTGAA ATTTGATTTC AATCTTATTT ATGAAGATAA AGATAGTATT GGTAAGCGTT ACAGAAGAAA TGATGCTTTA GGAACGCCAT TCTGTATTAC AATTGATCAT GATTCATTAA ACGACCATAC CGTAACACTC AGAGATAGGG ATACAATGCA GCAGGAAAGA GTTCCGGTTT CTGAATTAAG AAGAATCATT GATGATAAAG TTAACTTTAG AAACCTTTTG AGTAAAATA

Details for ElanA.18755.a.B1.GE41096

HARVESTED ON:	2/8/2017
SEQUENCED ON:	2/13/2017
EXPECTED MW:	60kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMA KQEDVFKKVI SHAKEYGFIF PSSEIYDGLS AIYDYGQNGA ELKNNIKQYW WKAMVQLNEN IVGIDSAIFM HPTIWKASGH VDAFNDPLID NKDSKKRFRA DVLVEDYCAK LEDKAQKEID KAAKRFGEAF DKAQFEATNP RVIEYREKQK TILSRLAKSL ENEDLADVKS LIEELEIADP DTGSKNWTEV RQFNLMFGTK LGATTETAMD LYLRPETAQG IFVNXLNVQK TSRHKLPFGI AXXGKAFRNX XVARQFXFRM REFEQMEMQF FVPPGTELEY YEKWKQTRLK WHLALGLGEE NYKFHDHEKL AHYANAAADI EFKFPFGFKE LEGIHSRTDF DLSAHEKFSG KKLQYFDPER NESYVPYVVE TSVGLDRLFL SILSHCLKDE VLEDGSERTV LSLPPALAPV KAAILPLMKK DGLGEYAEKI FNDLKFDFNL IYEDKDSIGK RYRRNDALGT PFCITIDHDS LNDHTVTLRD RDTMQQERVP VSELRRIIDD KVN

Validated NT Sequence

aaagttaact ttatcatcaa tgattcttct taattcagaa accggaactc tttcctgctg cattgtatcc ctatctctga gtgttacggt atggtcgttt aatgaatcat gatcaattgt aatacagaat ggcgttccta aagcatcatt tcttctgtaa cgcttaccaa tactatcttt atcttcataa ataagattga aatcaaattt caaatcgtta aagatttttt cagcatactc acctaaaccg tcttttttca ttaacggaag aatagcagct tttactggtg ctaaagccgg aggaagagaa agaacagttc tttcagatcc atcctctagt acttcatctt tcaggcaatg agaaaggata gaaaggaata atctgtccaa acctacagaa gtttccacta cgtatggcac atagctttca tttctttccg gatcaaaata ttgtagtttt ttaccagaga atttctcatg tgcagaaagg tcaaaatcag ttctgctgtg aataccttcc aattctttga atccaaatgg gaatttaaat tctatatcag ccgcagcatt tgcataatgc gctagtttct catgatcgtg gaatttgtag ttctcttcac caaggcctaa agccaggtgc catttcaaac gggtttgttt ccatttttca tagtattcaa gctctgttcc aggaggtacg aagaactgca tttccatttg ttcgaattca cgcattctga agntaaattg tctggcaacn nnntcgttac ggaatgcttt acctannnng gcaataccaa aaggcagctt atgacgagat gttttctgaa cgttcaggna atttacaaaa ataccttgtg ctgtttccgg acgaagataa agatccattg ccgtttctgt agtagcacca agcttagtac cgaacatcag gttaaattgt cttacttctg tccagttttt agaacctgta tccggatctg caatttccaa ttcttcaatc agagatttta catctgccag atcttcattt tcaagagatt ttgccaaacg agacagaatt gttttctgct tctctctgta ttctattact cttgggtttg tagcttcaaa ctgagcttta tcaaaagctt ctccaaaacg ttttgctgct ttatcaatct ctttctgagc tttatcttct aatttcgcac aatagtcttc taccagaaca tctgcacgga aacgtttttt agaatcttta ttatcaatta atggatcatt gaaagcgtcc acgtgtccgg aagccttcca gattgtaggg tgcataaaga ttgctgaatc gattcccaca atattttcgt tcagttgtac cattgctttc caccaatact gcttgatatt gtttttcagc tcagcaccgt tctgtccgta gtcatagatt gcagaaagcc cgtcataaat ttcacttgac ggaaatataa aaccatactc tttcgcgtga gaaatcacct tcttgaaaac gtcttcctgt ttagccatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHMA KQEDVFKKVI SHAKEYGFIF PSSEIYDGLS AIYDYGQNGA ELKNNIKQYW WKAMVQLNEN IVGIDSAIFM HPTIWKASGH VDAFNDPLID NKDSKKRFRA DVLVEDYCAK LEDKAQKEID KAAKRFGEAF DKAQFEATNP RVIEYREKQK TILSRLAKSL ENEDLADVKS LIEELEIADP DTGSKNWTEV RQFNLMFGTK LGATTETAMD LYLRPETAQG IFVNYLNVQK TSRHKLPFGI AQIGKAFRNE IVARQFIFRM REFEQMEMQF FVPPGTELEY YEKWKQTRLK WHLALGLGEE NYKFHDHEKL AHYANAAADI EFKFPFGFKE LEGIHSRTDF DLSAHEKFSG KKLQYFDPER NESYVPYVVE TSVGLDRLFL SILSHCLKDE VLEDGSERTV LSLPPALAPV KAAILPLMKK DGLGEYAEKI FNDLKFDFNL IYEDKDSIGK RYRRNDALGT PFCITIDHDS LNDHTVTLRD RDTMQQERVP VSELRRIIDD KVNFRNLLSK I

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta aacaggaaga cgttttcaag aaggtgattt ctcacgcgaa agagtatggt tttatatttc cgtcaagtga aatttatgac gggctttctg caatctatga ctacggacag aacggtgctg agctgaaaaa caatatcaag cagtattggt ggaaagcaat ggtacaactg aacgaaaata ttgtgggaat cgattcagca atctttatgc accctacaat ctggaaggct tccggacacg tggacgcttt caatgatcca ttaattgata ataaagattc taaaaaacgt ttccgtgcag atgttctggt agaagactat tgtgcgaaat tagaagataa agctcagaaa gagattgata aagcagcaaa acgttttgga gaagcttttg ataaagctca gtttgaagct acaaacccaa gagtaataga atacagagag aagcagaaaa caattctgtc tcgtttggca aaatctcttg aaaatgaaga tctggcagat gtaaaatctc tgattgaaga attggaaatt gcagatccgg atacaggttc taaaaactgg acagaagtaa gacaatttaa cctgatgttc ggtactaagc ttggtgctac tacagaaacg gcaatggatc tttatcttcg tccggaaaca gcacaaggta tttttgtaaa ttacctgaac gttcagaaaa catctcgtca taagctgcct tttggtattg cccaaatagg taaagcattc cgtaacgaaa ttgttgccag acaatttatc ttcagaatgc gtgaattcga acaaatggaa atgcagttct tcgtacctcc tggaacagag cttgaatact atgaaaaatg gaaacaaacc cgtttgaaat ggcacctggc tttaggcctt ggtgaagaga actacaaatt ccacgatcat gagaaactag cgcattatgc aaatgctgcg gctgatatag aatttaaatt cccatttgga ttcaaagaat tggaaggtat tcacagcaga actgattttg acctttctgc acatgagaaa ttctctggta aaaaactaca atattttgat ccggaaagaa atgaaagcta tgtgccatac gtagtggaaa cttctgtagg tttggacaga ttattccttt ctatcctttc tcattgcctg aaagatgaag tactagagga tggatctgaa agaactgttc tttctcttcc tccggcttta gcaccagtaa aagctgctat tcttccgtta atgaaaaaag acggtttagg tgagtatgct gaaaaaatct ttaacgattt gaaatttgat ttcaatctta tttatgaaga taaagatagt attggtaagc gttacagaag aaatgatgct ttaggaacgc cattctgtat tacaattgat catgattcat taaacgacca taccgtaaca ctcagagata gggatacaat gcagcaggaa agagttccgg tttctgaatt aagaagaatc attgatgata aagttaactt tagaaacctt ttgagtaaaa tatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for ElanA.18755.a.B1.PW38249

PURIFICATION DATe:	4/10/2017
CONCENTRATION:	9.5mg/ml
OBSERVED MW:	58kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	32
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMA KQEDVFKKVI SHAKEYGFIF PSSEIYDGLS AIYDYGQNGA ELKNNIKQYW WKAMVQLNEN IVGIDSAIFM HPTIWKASGH VDAFNDPLID NKDSKKRFRA DVLVEDYCAK LEDKAQKEID KAAKRFGEAF DKAQFEATNP RVIEYREKQK TILSRLAKSL ENEDLADVKS LIEELEIADP DTGSKNWTEV RQFNLMFGTK LGATTETAMD LYLRPETAQG IFVNYLNVQK TSRHKLPFGI AQIGKAFRNE IVARQFIFRM REFEQMEMQF FVPPGTELEY YEKWKQTRLK WHLALGLGEE NYKFHDHEKL AHYANAAADI EFKFPFGFKE LEGIHSRTDF DLSAHEKFSG KKLQYFDPER NESYVPYVVE TSVGLDRLFL SILSHCLKDE VLEDGSERTV LSLPPALAPV KAAILPLMKK DGLGEYAEKI FNDLKFDFNL IYEDKDSIGK RYRRNDALGT PFCITIDHDS LNDHTVTLRD RDTMQQERVP VSELRRIIDD KVNFRNLLSK I

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTA AACAGGAAGA CGTTTTCAAG AAGGTGATTT CTCACGCGAA AGAGTATGGT TTTATATTTC CGTCAAGTGA AATTTATGAC GGGCTTTCTG CAATCTATGA CTACGGACAG AACGGTGCTG AGCTGAAAAA CAATATCAAG CAGTATTGGT GGAAAGCAAT GGTACAACTG AACGAAAATA TTGTGGGAAT CGATTCAGCA ATCTTTATGC ACCCTACAAT CTGGAAGGCT TCCGGACACG TGGACGCTTT CAATGATCCA TTAATTGATA ATAAAGATTC TAAAAAACGT TTCCGTGCAG ATGTTCTGGT AGAAGACTAT TGTGCGAAAT TAGAAGATAA AGCTCAGAAA GAGATTGATA AAGCAGCAAA ACGTTTTGGA GAAGCTTTTG ATAAAGCTCA GTTTGAAGCT ACAAACCCAA GAGTAATAGA ATACAGAGAG AAGCAGAAAA CAATTCTGTC TCGTTTGGCA AAATCTCTTG AAAATGAAGA TCTGGCAGAT GTAAAATCTC TGATTGAAGA ATTGGAAATT GCAGATCCGG ATACAGGTTC TAAAAACTGG ACAGAAGTAA GACAATTTAA CCTGATGTTC GGTACTAAGC TTGGTGCTAC TACAGAAACG GCAATGGATC TTTATCTTCG TCCGGAAACA GCACAAGGTA TTTTTGTAAA TTACCTGAAC GTTCAGAAAA CATCTCGTCA TAAGCTGCCT TTTGGTATTG CCCAAATAGG TAAAGCATTC CGTAACGAAA TTGTTGCCAG ACAATTTATC TTCAGAATGC GTGAATTCGA ACAAATGGAA ATGCAGTTCT TCGTACCTCC TGGAACAGAG CTTGAATACT ATGAAAAATG GAAACAAACC CGTTTGAAAT GGCACCTGGC TTTAGGCCTT GGTGAAGAGA ACTACAAATT CCACGATCAT GAGAAACTAG CGCATTATGC AAATGCTGCG GCTGATATAG AATTTAAATT CCCATTTGGA TTCAAAGAAT TGGAAGGTAT TCACAGCAGA ACTGATTTTG ACCTTTCTGC ACATGAGAAA TTCTCTGGTA AAAAACTACA ATATTTTGAT CCGGAAAGAA ATGAAAGCTA TGTGCCATAC GTAGTGGAAA CTTCTGTAGG TTTGGACAGA TTATTCCTTT CTATCCTTTC TCATTGCCTG AAAGATGAAG TACTAGAGGA TGGATCTGAA AGAACTGTTC TTTCTCTTCC TCCGGCTTTA GCACCAGTAA AAGCTGCTAT TCTTCCGTTA ATGAAAAAAG ACGGTTTAGG TGAGTATGCT GAAAAAATCT TTAACGATTT GAAATTTGAT TTCAATCTTA TTTATGAAGA TAAAGATAGT ATTGGTAAGC GTTACAGAAG AAATGATGCT TTAGGAACGC CATTCTGTAT TACAATTGAT CATGATTCAT TAAACGACCA TACCGTAACA CTCAGAGATA GGGATACAAT GCAGCAGGAA AGAGTTCCGG TTTCTGAATT AAGAAGAATC ATTGATGATA AAGTTAACTT TAGAAACCTT TTGAGTAAAA TATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT