ElanA.18549.a

Phenylalanyl-tRNA synthetase alpha chain

CENTER ID: ElanA.18549.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.18549.a.B1.GE41094 Full length(ElanA.18549.a) 1 344
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.18549.a.B1.PW38392 Full length(ElanA.18549.a) 1 344

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.3002
UniProt: A0A077EH75

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNNISNLINE KRSKKLHNNK LHPVCQLKEK IQLYFNDFSV FDDLNEIVSI KDNFDDLLIP LDHPARSTND TYYADNDHVL RTQTSAHQNM LLKAGHKRFI ASGDVYRKDT IDKTHYPVFH QMEGVKILPA GADALDDLKK TLEGLIQYLY PGKEYRFLDD YFPFTEPSLQ IEVLQNKEWM EVLGAGVIHP QILKNCNIEG TGWAFGLGLD RLLLSYCNIP DIRYLWTDDL RFISQFENGL TEFHQYSKYP PVYKDISFWV NEYTENKEGN WNEYNNLCEV IREEGNDLVE SIDLLDKFCK ENKTSLAYRI MYRSNERTLL NDEINEKQER IRATLSENFD IILR
NT Sequence
ATGAACAATA TATCAAATCT AATAAATGAA AAGCGCAGTA AAAAACTGCA TAACAATAAA CTACACCCTG TATGCCAGTT AAAAGAAAAG ATACAATTAT ACTTCAATGA TTTTTCAGTA TTTGATGATT TAAATGAAAT TGTAAGTATT AAGGACAACT TTGATGATTT ACTGATTCCG TTGGATCATC CTGCCCGATC TACAAATGAT ACATATTATG CAGATAATGA TCATGTCCTT AGAACACAAA CTTCTGCACA CCAGAATATG CTGCTTAAGG CTGGCCATAA AAGATTCATT GCTTCGGGCG ATGTATACCG CAAAGACACT ATTGACAAAA CTCATTATCC GGTTTTCCAT CAGATGGAAG GTGTAAAAAT TTTACCCGCC GGAGCTGATG CTCTGGATGA TTTAAAGAAA ACACTGGAAG GATTGATTCA GTATTTATAT CCCGGAAAAG AATATAGATT TTTAGATGAT TATTTTCCTT TTACAGAACC TTCATTACAA ATAGAAGTAT TACAAAATAA AGAATGGATG GAAGTATTGG GAGCAGGTGT AATACATCCT CAAATTCTAA AAAACTGCAA TATAGAAGGT ACCGGCTGGG CTTTTGGGTT AGGTCTGGAT CGTTTGCTGC TTTCTTATTG TAATATCCCC GATATACGGT ATTTATGGAC AGACGATTTA CGTTTCATTT CACAATTCGA AAATGGATTG ACCGAATTTC ACCAATATTC AAAATATCCA CCTGTGTATA AAGACATATC ATTCTGGGTA AACGAATATA CTGAGAATAA AGAAGGCAAC TGGAATGAAT ATAATAATCT CTGTGAAGTT ATACGAGAAG AAGGTAATGA TCTTGTAGAA AGCATTGACC TGCTGGACAA ATTTTGTAAA GAAAACAAAA CATCTTTAGC TTATCGCATT ATGTACAGGT CTAATGAAAG AACCTTGCTA AATGATGAAA TTAATGAAAA ACAGGAACGT ATCAGAGCTA CATTGTCTGA AAATTTTGAC ATTATACTAA GA
Details for ElanA.18549.a.B1.GE41094
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 41kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMN NISNLINEKR SKKLHNNKLH PVCQLKEKIQ LYFNDFSVFD DLNEIVSIKD NFDDLLIPLD HPARSTNDTY YADNDHVLRT QTSAHQNMLL KAGHKRFIAS GDVYRKDTID KTHYPVFHQM EGVKILPAGA DALDDLKKTL EGLIQYLYPG KEYRFLDDYF PFTEPSLQIE VLQNKEWMEV LGAGVIHPQI LKNCNIEGTG WAFGLGLDRL LLSYCNIPDI RYLWTDDLRF ISQFENGLTE FHQYSKYPPV YKDISFWVNE YTENKEGNWN EYNNLCEVIR EEGNDLVESI DLLDKFCKEN KTSLAYRIMY RSNERTLLND EINEKQERIR ATLS
Validated NT Sequence
ttcagacaat gtagctctga tacgttcctg tttttcatta atttcatcat ttagcaaggt tctttcatta gacctgtaca taatgcgata agctaaagat gttttgtttt ctttacaaaa tttgtccagc aggtcaatgc tttctacaag atcattacct tcttctcgta taacttcaca gagattatta tattcattcc agttgccttc tttattctca gtatattcgt ttacccagaa tgatatgtct ttatacacag gtggatattt tgaatattgg tgaaattcgg tcaatccatt ttcgaattgt gaaatgaaac gtaaatcgtc tgtccataaa taccgtatat cggggatatt acaataagaa agcagcaaac gatccagacc taacccaaaa gcccagccgg taccttctat attgcagttt tttagaattt gaggatgtat tacacctgct cccaatactt ccatccattc tttattttgt aatacttcta tttgtaatga aggttctgta aaaggaaaat aatcatctaa aaatctatat tcttttccgg gatataaata ctgaatcaat ccttccagtg ttttctttaa atcatccaga gcatcagctc cggcgggtaa aatttttaca ccttccatct gatggaaaac cggataatga gttttgtcaa tagtgtcttt gcggtataca tcgcccgaag caatgaatct tttatggcca gccttaagca gcatattctg gtgtgcagaa gtttgtgttc taaggacatg atcattatct gcataatatg tatcatttgt agatcgggca ggatgatcca acggaatcag taaatcatca aagttgtcct taatacttac aatttcattt aaatcatcaa atactgaaaa atcattgaag tataattgta tcttttcttt taactggcat acagggtgta gtttattgtt atgcagtttt ttactgcgct tttcatttat tagatttgat atattgttca tatggtggtg gtggtggtga gccatggtat at
Expected Protein Sequence
MAHHHHHHMN NISNLINEKR SKKLHNNKLH PVCQLKEKIQ LYFNDFSVFD DLNEIVSIKD NFDDLLIPLD HPARSTNDTY YADNDHVLRT QTSAHQNMLL KAGHKRFIAS GDVYRKDTID KTHYPVFHQM EGVKILPAGA DALDDLKKTL EGLIQYLYPG KEYRFLDDYF PFTEPSLQIE VLQNKEWMEV LGAGVIHPQI LKNCNIEGTG WAFGLGLDRL LLSYCNIPDI RYLWTDDLRF ISQFENGLTE FHQYSKYPPV YKDISFWVNE YTENKEGNWN EYNNLCEVIR EEGNDLVESI DLLDKFCKEN KTSLAYRIMY RSNERTLLND EINEKQERIR ATLSENFDII LR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca atatatcaaa tctaataaat gaaaagcgca gtaaaaaact gcataacaat aaactacacc ctgtatgcca gttaaaagaa aagatacaat tatacttcaa tgatttttca gtatttgatg atttaaatga aattgtaagt attaaggaca actttgatga tttactgatt ccgttggatc atcctgcccg atctacaaat gatacatatt atgcagataa tgatcatgtc cttagaacac aaacttctgc acaccagaat atgctgctta aggctggcca taaaagattc attgcttcgg gcgatgtata ccgcaaagac actattgaca aaactcatta tccggttttc catcagatgg aaggtgtaaa aattttaccc gccggagctg atgctctgga tgatttaaag aaaacactgg aaggattgat tcagtattta tatcccggaa aagaatatag atttttagat gattattttc cttttacaga accttcatta caaatagaag tattacaaaa taaagaatgg atggaagtat tgggagcagg tgtaatacat cctcaaattc taaaaaactg caatatagaa ggtaccggct gggcttttgg gttaggtctg gatcgtttgc tgctttctta ttgtaatatc cccgatatac ggtatttatg gacagacgat ttacgtttca tttcacaatt cgaaaatgga ttgaccgaat ttcaccaata ttcaaaatat ccacctgtgt ataaagacat atcattctgg gtaaacgaat atactgagaa taaagaaggc aactggaatg aatataataa tctctgtgaa gttatacgag aagaaggtaa tgatcttgta gaaagcattg acctgctgga caaattttgt aaagaaaaca aaacatcttt agcttatcgc attatgtaca ggtctaatga aagaaccttg ctaaatgatg aaattaatga aaaacaggaa cgtatcagag ctacattgtc tgaaaatttt gacattatac taagatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for ElanA.18549.a.B1.PW38392
PURIFICATION DATe: 11/15/2017
CONCENTRATION: 34.16mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
UV lamp died no SEC profile
Validated AA Sequence
MAHHHHHHMN NISNLINEKR SKKLHNNKLH PVCQLKEKIQ LYFNDFSVFD DLNEIVSIKD NFDDLLIPLD HPARSTNDTY YADNDHVLRT QTSAHQNMLL KAGHKRFIAS GDVYRKDTID KTHYPVFHQM EGVKILPAGA DALDDLKKTL EGLIQYLYPG KEYRFLDDYF PFTEPSLQIE VLQNKEWMEV LGAGVIHPQI LKNCNIEGTG WAFGLGLDRL LLSYCNIPDI RYLWTDDLRF ISQFENGLTE FHQYSKYPPV YKDISFWVNE YTENKEGNWN EYNNLCEVIR EEGNDLVESI DLLDKFCKEN KTSLAYRIMY RSNERTLLND EINEKQERIR ATLS
Validated NT Sequence
ttcagacaat gtagctctga tacgttcctg tttttcatta atttcatcat ttagcaaggt tctttcatta gacctgtaca taatgcgata agctaaagat gttttgtttt ctttacaaaa tttgtccagc aggtcaatgc tttctacaag atcattacct tcttctcgta taacttcaca gagattatta tattcattcc agttgccttc tttattctca gtatattcgt ttacccagaa tgatatgtct ttatacacag gtggatattt tgaatattgg tgaaattcgg tcaatccatt ttcgaattgt gaaatgaaac gtaaatcgtc tgtccataaa taccgtatat cggggatatt acaataagaa agcagcaaac gatccagacc taacccaaaa gcccagccgg taccttctat attgcagttt tttagaattt gaggatgtat tacacctgct cccaatactt ccatccattc tttattttgt aatacttcta tttgtaatga aggttctgta aaaggaaaat aatcatctaa aaatctatat tcttttccgg gatataaata ctgaatcaat ccttccagtg ttttctttaa atcatccaga gcatcagctc cggcgggtaa aatttttaca ccttccatct gatggaaaac cggataatga gttttgtcaa tagtgtcttt gcggtataca tcgcccgaag caatgaatct tttatggcca gccttaagca gcatattctg gtgtgcagaa gtttgtgttc taaggacatg atcattatct gcataatatg tatcatttgt agatcgggca ggatgatcca acggaatcag taaatcatca aagttgtcct taatacttac aatttcattt aaatcatcaa atactgaaaa atcattgaag tataattgta tcttttcttt taactggcat acagggtgta gtttattgtt atgcagtttt ttactgcgct tttcatttat tagatttgat atattgttca tatggtggtg gtggtggtga gccatggtat at
Expressed Protein Sequence
MAHHHHHHMN NISNLINEKR SKKLHNNKLH PVCQLKEKIQ LYFNDFSVFD DLNEIVSIKD NFDDLLIPLD HPARSTNDTY YADNDHVLRT QTSAHQNMLL KAGHKRFIAS GDVYRKDTID KTHYPVFHQM EGVKILPAGA DALDDLKKTL EGLIQYLYPG KEYRFLDDYF PFTEPSLQIE VLQNKEWMEV LGAGVIHPQI LKNCNIEGTG WAFGLGLDRL LLSYCNIPDI RYLWTDDLRF ISQFENGLTE FHQYSKYPPV YKDISFWVNE YTENKEGNWN EYNNLCEVIR EEGNDLVESI DLLDKFCKEN KTSLAYRIMY RSNERTLLND EINEKQERIR ATLSENFDII LR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACA ATATATCAAA TCTAATAAAT GAAAAGCGCA GTAAAAAACT GCATAACAAT AAACTACACC CTGTATGCCA GTTAAAAGAA AAGATACAAT TATACTTCAA TGATTTTTCA GTATTTGATG ATTTAAATGA AATTGTAAGT ATTAAGGACA ACTTTGATGA TTTACTGATT CCGTTGGATC ATCCTGCCCG ATCTACAAAT GATACATATT ATGCAGATAA TGATCATGTC CTTAGAACAC AAACTTCTGC ACACCAGAAT ATGCTGCTTA AGGCTGGCCA TAAAAGATTC ATTGCTTCGG GCGATGTATA CCGCAAAGAC ACTATTGACA AAACTCATTA TCCGGTTTTC CATCAGATGG AAGGTGTAAA AATTTTACCC GCCGGAGCTG ATGCTCTGGA TGATTTAAAG AAAACACTGG AAGGATTGAT TCAGTATTTA TATCCCGGAA AAGAATATAG ATTTTTAGAT GATTATTTTC CTTTTACAGA ACCTTCATTA CAAATAGAAG TATTACAAAA TAAAGAATGG ATGGAAGTAT TGGGAGCAGG TGTAATACAT CCTCAAATTC TAAAAAACTG CAATATAGAA GGTACCGGCT GGGCTTTTGG GTTAGGTCTG GATCGTTTGC TGCTTTCTTA TTGTAATATC CCCGATATAC GGTATTTATG GACAGACGAT TTACGTTTCA TTTCACAATT CGAAAATGGA TTGACCGAAT TTCACCAATA TTCAAAATAT CCACCTGTGT ATAAAGACAT ATCATTCTGG GTAAACGAAT ATACTGAGAA TAAAGAAGGC AACTGGAATG AATATAATAA TCTCTGTGAA GTTATACGAG AAGAAGGTAA TGATCTTGTA GAAAGCATTG ACCTGCTGGA CAAATTTTGT AAAGAAAACA AAACATCTTT AGCTTATCGC ATTATGTACA GGTCTAATGA AAGAACCTTG CTAAATGATG AAATTAATGA AAAACAGGAA CGTATCAGAG CTACATTGTC TGAAAATTTT GACATTATAC TAAGATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT