ElanA.18222.a

Glutamine--tRNA ligase

CENTER ID: ElanA.18222.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.18222.a.B1.GE41093 Full length(ElanA.18222.a) 1 556
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.3692
UniProt: A0A077EGR2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEEEKKSLNF IEQIIEEDLA NGLEKDKIRF RFPPEPNGYL HIGHTKAICI NFGLGEKYNA PVNLRFDDTN PEKEEQEFVD SIKKDVEWLG FKWDKELYAS DYFQQLYDWA VQMIKEGKAY VDEQPSEVIT EQRKNPAEPG VESPYRNRPV EESLDLFEKM KNGEFESGSM SLRAKIDMIS PNMNMRDPVM YRILNKPHHR TGTTWKIYPM YDWAHGESDY LEQVSHSLCS LEFENHRPLY NWYLEQVRDE NKVAPKQREF ARMNVTYMIT SKRKLQKLIA ENVVTGWDDP RMPTVSGLRR KGYTAEAIRN FIERIGVAKR ENLIDIQLLE FFVREDLNKK AKRVMAVVDP VKLIIENYPE GQEEWVETEN NPEDENAGTR QIPFSREIYI EREDFKEEAN NKFFRLKLGG EVRLKSAYII KAERVEKDEN GEITTIYATY DDKSKSGSGT EESLRKVKGT LHWVSAPHAI PVEARLYERL FTVEQPDAEK DADFLQYVNP ESLNTAHGFA EPSLKDVEIG EPLQFQRIGY FTKDRDSSDE KLVFNRTVTL KDSYKP
NT Sequence
ATGGAAGAAG AAAAAAAATC TCTCAATTTT ATTGAGCAAA TTATAGAGGA AGATCTGGCA AACGGTCTTG AAAAAGATAA GATCCGTTTC CGTTTCCCAC CAGAACCGAA TGGTTATTTA CACATTGGCC ACACAAAGGC TATTTGTATC AACTTTGGTT TGGGTGAAAA ATATAATGCT CCGGTTAACC TGCGTTTTGA TGACACAAAT CCTGAAAAGG AAGAGCAGGA ATTTGTAGAT TCTATTAAGA AGGATGTAGA GTGGCTTGGC TTTAAGTGGG ACAAGGAACT TTATGCGTCC GACTATTTCC AACAATTATA TGACTGGGCT GTACAAATGA TTAAAGAAGG AAAGGCTTAT GTAGATGAGC AACCTTCCGA GGTAATCACT GAACAACGTA AAAATCCTGC TGAACCAGGA GTAGAATCAC CATATAGAAA CAGACCTGTG GAAGAATCTC TGGATTTATT TGAAAAAATG AAAAACGGAG AATTCGAAAG CGGATCAATG TCACTTCGTG CTAAAATCGA TATGATTTCG CCAAATATGA ACATGCGTGA CCCTGTTATG TATCGTATTC TGAATAAACC ACACCACAGA ACAGGAACTA CATGGAAAAT TTATCCGATG TATGACTGGG CTCATGGTGA ATCCGATTAT CTGGAGCAGG TATCACACTC ACTTTGTTCT TTGGAATTCG AAAATCACAG ACCATTATAC AACTGGTATC TTGAACAGGT AAGAGATGAA AATAAAGTAG CACCAAAGCA AAGAGAATTT GCCAGAATGA ATGTTACTTA TATGATTACC TCTAAAAGAA AGCTACAGAA GCTTATTGCA GAAAATGTAG TCACTGGATG GGACGATCCA AGAATGCCAA CTGTTTCCGG ATTGAGAAGA AAAGGTTATA CTGCAGAAGC TATACGTAAC TTTATCGAAC GTATTGGTGT TGCAAAACGC GAAAACCTTA TCGATATTCA GTTACTGGAA TTCTTCGTAC GTGAAGATCT GAACAAAAAG GCTAAAAGAG TAATGGCTGT TGTAGATCCC GTAAAACTGA TCATTGAAAA CTATCCGGAA GGACAGGAAG AATGGGTGGA GACTGAAAAT AATCCGGAAG ATGAAAATGC CGGAACCAGA CAAATTCCTT TCTCCAGAGA AATATACATT GAAAGAGAAG ACTTTAAAGA AGAAGCTAAT AATAAATTCT TCCGTCTGAA ACTAGGTGGA GAGGTTCGTC TAAAGTCTGC TTACATTATT AAAGCGGAGC GTGTAGAGAA AGATGAAAAT GGTGAAATCA CTACAATCTA TGCGACTTAC GATGATAAGA GTAAATCAGG CAGTGGTACA GAAGAAAGCC TGAGAAAAGT AAAAGGTACG TTACACTGGG TATCTGCACC TCATGCTATT CCTGTAGAAG CTCGTTTATA TGAACGCTTG TTTACTGTAG AGCAGCCGGA TGCAGAAAAG GATGCAGATT TCTTACAATA CGTTAACCCT GAGTCATTAA ATACAGCTCA TGGTTTTGCT GAGCCAAGCC TTAAAGATGT AGAAATCGGA GAACCTTTAC AGTTCCAGAG AATTGGTTAT TTCACTAAAG ATCGTGACTC TTCAGATGAA AAACTTGTTT TCAACAGAAC AGTAACTTTA AAAGATTCTT ACAAACCA
Details for ElanA.18222.a.B1.GE41093
HARVESTED ON: data unavailable
SEQUENCED ON: 2/13/2017
EXPECTED MW: 66kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHME EEKKSLNFIE QIIEEDLANG LEKDKIRFRF PPEPNGYLHI GHTKAICINF GLGEKYNAPV NLRFDDTNPE KEEQEFVDSI KKDVEWLGFK WDKELYASDY FQQLYDWAVQ MIKEGKAYVD EQPSEVITEQ RKNPAEPGVE SPYRNRPVEE SLDLFEKMKN GEFESGSMSL RAKIDMISPN MNMRDPVMYR ILNKPHHRTG TTWKIYPMYD WAHGESDYLE QVSHSLCSLE FENXRPLXXW YLEQVRDENK VAPKQRXXXX MNVTYXXXXK RKXXXLIAEN VVTGWDDPXX XTVSGLXXKG YTAEAIRNFI ERIGVAKREN LIDIQLLEFF VREDLNKKAK RVMAVVDPVK LIIENYPEGQ EEWVETENNP EDENAGTRQI PFSREIYIER EDFKEEANNK FFRLKLGGEV RLKSAYIIKA ERVEKDENGE ITTIYATYDD KSKSGSGTEE SLRKVKGTLH WVSAPHAIPV EARLYERLFT VEQPDAEKDA DFLQYVNPES LNTAHGFAEP SLKDVEIGEP LQFQRIGYFT KDRDSSDEKL VFNRTV
Validated NT Sequence
ttactgttct gttgaaaaca agtttttcat ctgaagagtc acgatcttta gtgaaataac caattctctg gaactgtaaa ggttctccga tttctacatc tttaaggctt ggctcagcaa aaccatgagc tgtatttaat gactcagggt taacgtattg taagaaatct gcatcctttt ctgcatccgg ctgctctaca gtaaacaagc gttcatataa acgagcttct acaggaatag catgaggtgc agatacccag tgtaacgtac cttttacttt tctcaggctt tcttctgtac cactgcctga tttactctta tcatcgtaag tcgcatagat tgtagtgatt tcaccatttt catctttctc tacacgctcc gctttaataa tgtaagcaga ctttagacga acctctccac ctagtttcag acggaagaat ttattattag cttcttcttt aaagtcttct ctttcaatgt atatttctct ggagaaagga atttgtctgg ttccggcatt ttcatcttcc ggattatttt cagtctccac ccattcttcc tgtccttccg gatagttttc aatgatcagt tttacgggat ctacaacagc cattactctt ttagcctttt tgttcagatc ttcacgtacg aagaattcca gtaactgaat atcgataagg ttttcgcgtt ttgcaacacc aatacgttcg ataaagttac gtatagcttc tgcagtataa ccttttcnnc tcaatccgga aacagttgnn nnnnntggat cgtcccatcc agtgactaca ttttctgcaa taagnnnnnn nnnctttctt ttnnnggnnn nnatataagt aacattcatt cnnncnnnnt ctctttgctt tggtgctact ttattttcat ctcttacctg ttcaagatac cagtnnnata atggtctnng attttcgaat tccaaagaac aaagtgagtg tgatacctgc tccagataat cggattcacc atgagcccag tcatacatcg gataaatttt ccatgtagtt cctgttctgt ggtgtggttt attcagaata cgatacataa cagggtcacg catgttcata tttggcgaaa tcatatcgat tttagcacga agtgacattg atccgctttc gaattctccg tttttcattt tttcaaataa atccagagat tcttccacag gtctgtttct atatggtgat tctactcctg gttcagcagg atttttacgt tgttcagtga ttacctcgga aggttgctca tctacataag cctttccttc tttaatcatt tgtacagccc agtcatataa ttgttggaaa tagtcggacg cataaagttc cttgtcccac ttaaagccaa gccactctac atccttctta atagaatcta caaattcctg ctcttccttt tcaggatttg tgtcatcaaa acgcaggtta accggagcat tatatttttc acccaaacca aagttgatac aaatagcctt tgtgtggcca atgtgtaaat aaccattcgg ttctggtggg aaacggaaac ggatcttatc tttttcaaga ccgtttgcca gatcttcctc tataatttgc tcaataaaat tgagagattt tttttcttct tccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHME EEKKSLNFIE QIIEEDLANG LEKDKIRFRF PPEPNGYLHI GHTKAICINF GLGEKYNAPV NLRFDDTNPE KEEQEFVDSI KKDVEWLGFK WDKELYASDY FQQLYDWAVQ MIKEGKAYVD EQPSEVITEQ RKNPAEPGVE SPYRNRPVEE SLDLFEKMKN GEFESGSMSL RAKIDMISPN MNMRDPVMYR ILNKPHHRTG TTWKIYPMYD WAHGESDYLE QVSHSLCSLE FENHRPLYNW YLEQVRDENK VAPKQREFAR MNVTYMITSK RKLQKLIAEN VVTGWDDPRM PTVSGLRRKG YTAEAIRNFI ERIGVAKREN LIDIQLLEFF VREDLNKKAK RVMAVVDPVK LIIENYPEGQ EEWVETENNP EDENAGTRQI PFSREIYIER EDFKEEANNK FFRLKLGGEV RLKSAYIIKA ERVEKDENGE ITTIYATYDD KSKSGSGTEE SLRKVKGTLH WVSAPHAIPV EARLYERLFT VEQPDAEKDA DFLQYVNPES LNTAHGFAEP SLKDVEIGEP LQFQRIGYFT KDRDSSDEKL VFNRTVTLKD SYKP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag aagaaaaaaa atctctcaat tttattgagc aaattataga ggaagatctg gcaaacggtc ttgaaaaaga taagatccgt ttccgtttcc caccagaacc gaatggttat ttacacattg gccacacaaa ggctatttgt atcaactttg gtttgggtga aaaatataat gctccggtta acctgcgttt tgatgacaca aatcctgaaa aggaagagca ggaatttgta gattctatta agaaggatgt agagtggctt ggctttaagt gggacaagga actttatgcg tccgactatt tccaacaatt atatgactgg gctgtacaaa tgattaaaga aggaaaggct tatgtagatg agcaaccttc cgaggtaatc actgaacaac gtaaaaatcc tgctgaacca ggagtagaat caccatatag aaacagacct gtggaagaat ctctggattt atttgaaaaa atgaaaaacg gagaattcga aagcggatca atgtcacttc gtgctaaaat cgatatgatt tcgccaaata tgaacatgcg tgaccctgtt atgtatcgta ttctgaataa accacaccac agaacaggaa ctacatggaa aatttatccg atgtatgact gggctcatgg tgaatccgat tatctggagc aggtatcaca ctcactttgt tctttggaat tcgaaaatca cagaccatta tacaactggt atcttgaaca ggtaagagat gaaaataaag tagcaccaaa gcaaagagaa tttgccagaa tgaatgttac ttatatgatt acctctaaaa gaaagctaca gaagcttatt gcagaaaatg tagtcactgg atgggacgat ccaagaatgc caactgtttc cggattgaga agaaaaggtt atactgcaga agctatacgt aactttatcg aacgtattgg tgttgcaaaa cgcgaaaacc ttatcgatat tcagttactg gaattcttcg tacgtgaaga tctgaacaaa aaggctaaaa gagtaatggc tgttgtagat cccgtaaaac tgatcattga aaactatccg gaaggacagg aagaatgggt ggagactgaa aataatccgg aagatgaaaa tgccggaacc agacaaattc ctttctccag agaaatatac attgaaagag aagactttaa agaagaagct aataataaat tcttccgtct gaaactaggt ggagaggttc gtctaaagtc tgcttacatt attaaagcgg agcgtgtaga gaaagatgaa aatggtgaaa tcactacaat ctatgcgact tacgatgata agagtaaatc aggcagtggt acagaagaaa gcctgagaaa agtaaaaggt acgttacact gggtatctgc acctcatgct attcctgtag aagctcgttt atatgaacgc ttgtttactg tagagcagcc ggatgcagaa aaggatgcag atttcttaca atacgttaac cctgagtcat taaatacagc tcatggtttt gctgagccaa gccttaaaga tgtagaaatc ggagaacctt tacagttcca gagaattggt tatttcacta aagatcgtga ctcttcagat gaaaaacttg ttttcaacag aacagtaact ttaaaagatt cttacaaacc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat