ElanA.18132.a

5-methyltetrahydrofolate--homocysteine methyltransferase

CENTER ID: ElanA.18132.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.18132.a.B1.GE40673 Full length(ElanA.18132.a) 1 336
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.2952
UniProt: A0A077EP83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNIEALYRA LNERILILDG AMGTMLQRHK FEEEDFRGDR FKDWPSPLKG NNDLLSLTQP QAIEDVHRQY FEAGADIIET NTFSGTTIAM ADYGMEELVT ELNYESAKIA RKVADEYTAK TPDKPRFVAG AIGPTNKTAS LSPDVNDPGY RAITFDELKE AYKQQAKALL DGGSDILLVE TIFDTLNAKA ALFAIDEIQD ERNIRVPIMV SGTITDASGR TLSGQTAEAF LISVSHLNLL SVGFNCALGA RQLTPYLEVL SHNTDFAVSA YPNAGLPNAF GQYDETPEDM AEQIKEFVDK GLINIIGGCC GTTPEHIKAI ADLAKNYKPR VTSVKA
NT Sequence
ATGAGTAACA TAGAAGCATT ATATAGAGCA TTGAACGAGC GAATCCTTAT TCTGGATGGC GCTATGGGGA CAATGCTACA GCGACACAAA TTTGAAGAAG AAGATTTCAG AGGAGATCGC TTTAAAGACT GGCCAAGTCC TTTAAAAGGG AATAATGATC TGCTTTCTTT AACACAGCCA CAAGCTATTG AAGATGTGCA CAGACAATAT TTTGAAGCTG GTGCAGATAT TATCGAGACC AATACCTTTT CCGGGACTAC AATTGCAATG GCAGATTATG GGATGGAAGA GCTGGTAACA GAGCTTAACT ATGAATCAGC AAAAATAGCC AGAAAAGTTG CAGATGAGTA TACTGCAAAA ACACCAGATA AGCCTCGATT TGTTGCGGGA GCTATTGGTC CTACTAATAA AACCGCAAGC CTTTCTCCGG ATGTGAATGA TCCGGGATAT CGTGCCATTA CCTTTGACGA ACTTAAAGAG GCTTATAAAC AACAGGCTAA AGCTTTATTG GATGGAGGTT CTGATATTTT ACTGGTAGAA ACTATATTCG ATACGCTGAA TGCTAAAGCA GCACTTTTTG CGATCGACGA GATTCAGGAT GAAAGAAATA TCCGTGTACC AATTATGGTG AGTGGGACAA TTACGGATGC TTCCGGAAGA ACGCTTTCAG GGCAAACTGC TGAAGCTTTC CTTATTTCGG TTTCGCATCT TAATTTGCTA AGTGTTGGTT TCAATTGTGC TTTAGGAGCT CGGCAGCTAA CACCTTATCT GGAAGTATTA TCACATAATA CAGATTTTGC AGTTTCTGCT TATCCAAATG CAGGTTTACC GAACGCATTC GGGCAATATG ACGAAACACC GGAGGATATG GCAGAACAAA TTAAAGAGTT TGTAGATAAG GGATTAATCA ACATTATTGG AGGATGCTGC GGAACAACAC CAGAGCATAT TAAAGCCATT GCAGATTTAG CCAAAAACTA TAAGCCAAGA GTAACATCAG TTAAGGCT
Details for ElanA.18132.a.B1.GE40673
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS NIEALYRALN ERILILDGAM GTMLQRHKFE EEDFRGDRFK DWPSPLKGNN DLLSLTQPQA IEDVHRQYFE AGADIIETNT FSGTTIAMAD YGMEELVTEL NYESAKIARK VADEYTAKTP DKPRFVAGAI GPTNKTASLS PDVNDPGYRA ITFDELKEAY KQQAKALLDG GSDILLVETI FDTLNAKAAL FAIDEIQDER NIRVPIMVSG TITDASGRTL SGQTAEAFLI SVSHLNLLSV GFNCALGARQ LTPYLEVLSH NTDFAVSAYP NAGLPNAFGQ YDETPEDMAE QIKEFVDKGL INIIGGCCGT TPEHIKAIAD LAKNYK
Validated NT Sequence
atggctcacc accaccacca ccatatgagt aacatagaag cattatatag agcattgaac gagcgaatcc ttattctgga tggcgctatg gggacaatgc tacagcgaca caaatttgaa gaagaagatt tcagaggaga tcgctttaaa gactggccaa gtcctttaaa agggaataat gatctgcttt ctttaacaca gccacaagct attgaagatg tgcacagaca atattttgaa gctggtgcag atattatcga gaccaatacc ttttccggga ctacaattgc aatggcagat tatgggatgg aagagctggt aacagagctt aactatgaat cagcaaaaat agccagaaaa gttgcagatg agtatactgc aaaaacacca gataagcctc gatttgttgc gggagctatt ggtcctacta ataaaaccgc aagcctttct ccggatgtga atgatccggg atatcgtgcc attacctttg acgaacttaa agaggcttat aaacaacagg ctaaagcttt attggatgga ggttctgata ttttactggt agaaactata ttcgatacgc tgaatgctaa agcagcactt tttgcgatcg acgagattca ggatgaaaga aatatccgtg taccaattat ggtgagtggg acaattacgg atgcttccgg aagaacgctt tcagggcaaa ctgctgaagc tttccttatt tcggtttcgc atcttaattt gctaagtgtt ggtttcaatt gtgctttagg agctcggcag ctaacacctt atctggaagt attatcacat aatacagatt ttgcagtttc tgcttatcca aatgcaggtt taccgaacgc attcgggcaa tatgacgaaa caccggagga tatggcagaa caaattaaag agtttgtaga taagggatta atcaacatta ttggaggatg ctgcggaaca acaccagagc atattaaagc cattgcagat ttagccaaaa actataagcc a
Expected Protein Sequence
MAHHHHHHMS NIEALYRALN ERILILDGAM GTMLQRHKFE EEDFRGDRFK DWPSPLKGNN DLLSLTQPQA IEDVHRQYFE AGADIIETNT FSGTTIAMAD YGMEELVTEL NYESAKIARK VADEYTAKTP DKPRFVAGAI GPTNKTASLS PDVNDPGYRA ITFDELKEAY KQQAKALLDG GSDILLVETI FDTLNAKAAL FAIDEIQDER NIRVPIMVSG TITDASGRTL SGQTAEAFLI SVSHLNLLSV GFNCALGARQ LTPYLEVLSH NTDFAVSAYP NAGLPNAFGQ YDETPEDMAE QIKEFVDKGL INIIGGCCGT TPEHIKAIAD LAKNYKPRVT SVKA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta acatagaagc attatataga gcattgaacg agcgaatcct tattctggat ggcgctatgg ggacaatgct acagcgacac aaatttgaag aagaagattt cagaggagat cgctttaaag actggccaag tcctttaaaa gggaataatg atctgctttc tttaacacag ccacaagcta ttgaagatgt gcacagacaa tattttgaag ctggtgcaga tattatcgag accaatacct tttccgggac tacaattgca atggcagatt atgggatgga agagctggta acagagctta actatgaatc agcaaaaata gccagaaaag ttgcagatga gtatactgca aaaacaccag ataagcctcg atttgttgcg ggagctattg gtcctactaa taaaaccgca agcctttctc cggatgtgaa tgatccggga tatcgtgcca ttacctttga cgaacttaaa gaggcttata aacaacaggc taaagcttta ttggatggag gttctgatat tttactggta gaaactatat tcgatacgct gaatgctaaa gcagcacttt ttgcgatcga cgagattcag gatgaaagaa atatccgtgt accaattatg gtgagtggga caattacgga tgcttccgga agaacgcttt cagggcaaac tgctgaagct ttccttattt cggtttcgca tcttaatttg ctaagtgttg gtttcaattg tgctttagga gctcggcagc taacacctta tctggaagta ttatcacata atacagattt tgcagtttct gcttatccaa atgcaggttt accgaacgca ttcgggcaat atgacgaaac accggaggat atggcagaac aaattaaaga gtttgtagat aagggattaa tcaacattat tggaggatgc tgcggaacaa caccagagca tattaaagcc attgcagatt tagccaaaaa ctataagcca agagtaacat cagttaaggc ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat