ElanA.18022.a

Prolyl endopeptidase

CENTER ID: ElanA.18022.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.18022.a.B2.GE40670 Mature protein(ElanA.18022.a) 20 705
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.18022.a.B2.PW38263 Mature protein(ElanA.18022.a) 20 705

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.81
UniProt: A0A077ECJ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKYKKLSVAV AAFAFAAVSA QNSNSLKYPE TKKVNHTDTY FGNQVADPYR WLEDDRAEDT KAWVQQEVKF TQDYLAQIPF REQIKKQLLD IWNYEKISAP FKKGKYTYFY KNDGLQAQSV LYRKDASGKT GVFLDPNKFS DKGTTSLANL SFNKKGTLVA YSISEGGSDW NKIIILDAET KKQIDETLID VKFSGISWLG DEGFFYSSYD KPKDGSVLSG MTDKHKVYFH KLGTKQSQDE LIIGGDKFPR RYLSGYVTED QRYLVVSAAN ATNGNELYIK DLKNKTDFIP IVTGFDSNVG LVDTDGDTLF LHTDKNAPNM RMVKTTIQNP KPETWKDVIA ETSEPMRVNS GGGYFFATYM KDALSQIKQY DKTGKLVREI KLPGNGTAGG FGGEKTEKEL YYSFTNYITP PTIFKFNVDS GKSEVYQKPK VKFNPENYVS EQVFYTSADG TKIPMMISYK KGLKKDGKNP TILYSYGGFN ISLQPAFSVV NAIWMENGGI YAVPNIRGGG EYGKKWHDAG TKQQKKNVFN DFIAAGEYLQ KNGYTSKDYM ALSGRSNGGL LVGATMTMRP DLAKVAFPGV GVLDMLRYNK FTAGAGWAYD YGTAEDSKEM FEYLKSYSPV HNVKAGTCYP STMVITSDHD DRVVPAHSFK FGAELQAKQA CKNPVLIRIE TNAGHGAGRS TEQVVMENAD LLSFSLYEMG IKKLK
NT Sequence
ATGAAGTATA AAAAACTATC TGTGGCAGTT GCAGCTTTTG CTTTTGCAGC TGTTTCAGCA CAAAATTCTA ACTCATTGAA GTATCCCGAA ACAAAGAAAG TAAATCATAC CGATACCTAT TTTGGTAATC AGGTAGCAGA CCCATACCGT TGGCTGGAAG ATGACAGAGC TGAAGATACC AAAGCATGGG TACAGCAGGA GGTAAAATTT ACGCAGGATT ATCTTGCACA GATTCCTTTC CGCGAACAGA TTAAAAAGCA ACTTTTAGAT ATCTGGAATT ATGAAAAGAT TTCAGCTCCA TTTAAAAAAG GTAAATACAC TTATTTCTAC AAAAATGATG GACTGCAAGC ACAGTCTGTG TTATACAGAA AAGATGCTTC CGGAAAAACA GGAGTCTTTT TAGATCCGAA TAAATTTTCG GATAAAGGAA CAACCTCTTT GGCTAATCTT TCATTCAACA AAAAAGGAAC ACTAGTCGCT TACAGCATTT CTGAAGGAGG CTCTGACTGG AATAAGATTA TCATTCTGGA TGCGGAAACT AAAAAACAGA TCGACGAGAC ACTTATTGAT GTAAAATTTA GTGGAATTTC CTGGTTAGGA GATGAAGGCT TCTTCTATTC CAGCTATGAT AAACCAAAAG ACGGAAGTGT ACTTTCCGGA ATGACGGATA AGCACAAGGT ATATTTTCAC AAGCTTGGAA CAAAGCAGTC TCAGGATGAG CTGATTATTG GAGGAGATAA ATTCCCGAGA AGATATTTAA GTGGTTATGT GACAGAAGAT CAGAGGTATC TTGTTGTTTC TGCAGCTAAC GCAACCAACG GGAACGAACT CTATATTAAA GATCTTAAGA ATAAAACAGA TTTTATCCCA ATTGTTACAG GTTTTGATAG CAATGTAGGT TTGGTTGATA CAGATGGAGA TACACTGTTC CTGCATACTG ATAAAAATGC GCCTAATATG CGCATGGTAA AAACAACAAT TCAGAATCCG AAGCCGGAAA CCTGGAAAGA TGTTATCGCT GAAACATCTG AACCTATGCG TGTTAACTCA GGCGGAGGTT ATTTCTTTGC GACTTACATG AAGGATGCGC TAAGCCAGAT TAAACAATAC GATAAAACCG GTAAGCTGGT TAGGGAAATC AAACTGCCGG GTAACGGTAC TGCTGGTGGC TTTGGTGGTG AAAAAACAGA AAAAGAACTG TATTATTCAT TTACCAACTA CATTACGCCT CCAACGATCT TTAAATTTAA TGTAGATTCA GGAAAATCTG AGGTTTACCA AAAGCCAAAA GTAAAATTCA ATCCGGAAAA TTATGTTTCT GAGCAGGTAT TCTATACATC TGCAGACGGC ACTAAAATTC CAATGATGAT CAGTTATAAA AAAGGACTGA AAAAAGACGG GAAAAATCCA ACAATACTAT ACAGCTACGG AGGATTTAAT ATCAGTCTAC AACCTGCATT CTCTGTGGTA AATGCTATCT GGATGGAAAA TGGCGGAATC TATGCTGTTC CGAATATCCG TGGTGGCGGT GAATACGGAA AAAAATGGCA TGATGCAGGA ACCAAACAGC AAAAGAAAAA TGTTTTTAAT GACTTTATAG CTGCTGGGGA ATATTTACAG AAGAACGGTT ATACTTCCAA AGATTATATG GCACTTTCAG GACGTTCGAA TGGGGGATTG CTTGTTGGTG CTACAATGAC AATGCGCCCG GATCTAGCTA AAGTAGCTTT CCCTGGTGTT GGTGTACTGG ATATGCTGCG TTATAATAAA TTTACAGCAG GTGCCGGATG GGCATATGAC TACGGTACAG CCGAGGATAG TAAAGAAATG TTTGAGTATC TGAAATCTTA TTCACCTGTA CACAATGTAA AAGCTGGTAC ATGTTACCCT TCTACAATGG TAATTACGAG TGATCATGAT GACAGAGTTG TTCCTGCGCA TTCATTTAAA TTTGGTGCAG AATTACAGGC AAAACAAGCA TGTAAGAACC CGGTTCTTAT CCGTATTGAG ACTAATGCGG GACACGGAGC AGGACGCTCT ACTGAACAAG TAGTGATGGA AAATGCAGAT TTGCTTTCAT TCTCATTATA TGAAATGGGT ATTAAAAAGC TGAAG
Details for ElanA.18022.a.B2.GE40670
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 78kDa
OBSERVED MW: 77kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHAQ NSNSLKYPET KKVNHTDTYF GNQVADPYRW LEDDRAEDTK AWVQQEVKFT QDYLAQIPFR EQIKKQLLDI WNYEKISAPF KKGKYTYFYK NDGLQAQSVL YRKDASGKTG VFLDPNKFSD KGTTSLANLS FNKKGTLVAY SISEGGSDWN KIIILDAETK KQIDETLIDV KFSGISWLGD EGFFYSSYDK PKDGSVLSGM TDKHKVYFHK LGTKQSQDEL IIGGDKFPRR YLSGYVTEDQ RYLVVSAANA TNGNELYIKD LKNKTDFIPI VTGFDSNVGL VDTDGDTLFL HTDKNAPNMR MVKTTIQNPK PETWKDVIAE TSEPMRVNSG GGYFFATYMK DALSQIKQYD KTGKLVREIK LPGNGTAGGF GGEKTEKELY YSFTNYITPP TIFKFNVDSG KSEVYQKPKV KFNPENYVSE QVFYTSADGT KIPMMISYKK GLKKDGKNPT ILYSYGGFNI SLQPAFSVVN AIWMENGGIY AVPNIRGGGE YGKKWHDAGT KQQKKNVFND FIAAGEYLQK NGYTSKDYMA LSGRSNGGLL VGATMTMRPD LAKVAFPGVG VLDMLRYNKF TAGAGWAYDY GTAEDSKEMF EYLKSYSPVH NVKAGTCYPS TMVITSDHDD RVVPAHSFKF GAELQAKQAC KNPVLIRIET NAGHGAGRST EQVVMENADL LSFSLYEMGI KKLK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcacaaa attctaactc attgaagtat cccgaaacaa agaaagtaaa tcataccgat acctattttg gtaatcaggt agcagaccca taccgttggc tggaagatga cagagctgaa gataccaaag catgggtaca gcaggaggta aaatttacgc aggattatct tgcacagatt cctttccgcg aacagattaa aaagcaactt ttagatatct ggaattatga aaagatttca gctccattta aaaaaggtaa atacacttat ttctacaaaa atgatggact gcaagcacag tctgtgttat acagaaaaga tgcttccgga aaaacaggag tctttttaga tccgaataaa ttttcggata aaggaacaac ctctttggct aatctttcat tcaacaaaaa aggaacacta gtcgcttaca gcatttctga aggaggctct gactggaata agattatcat tctggatgcg gaaactaaaa aacagatcga cgagacactt attgatgtaa aatttagtgg aatttcctgg ttaggagatg aaggcttctt ctattccagc tatgataaac caaaagacgg aagtgtactt tccggaatga cggataagca caaggtatat tttcacaagc ttggaacaaa gcagtctcag gatgagctga ttattggagg agataaattc ccgagaagat atttaagtgg ttatgtgaca gaagatcaga ggtatcttgt tgtttctgca gctaacgcaa ccaacgggaa cgaactctat attaaagatc ttaagaataa aacagatttt atcccaattg ttacaggttt tgatagcaat gtaggtttgg ttgatacaga tggagataca ctgttcctgc atactgataa aaatgcgcct aatatgcgca tggtaaaaac aacaattcag aatccgaagc cggaaacctg gaaagatgtt atcgctgaaa catctgaacc tatgcgtgtt aactcaggcg gaggttattt ctttgcgact tacatgaagg atgcgctaag ccagattaaa caatacgata aaaccggtaa gctggttagg gaaatcaaac tgccgggtaa cggtactgct ggtggctttg gtggtgaaaa aacagaaaaa gaactgtatt attcatttac caactacatt acgcctccaa cgatctttaa atttaatgta gattcaggaa aatctgaggt ttaccaaaag ccaaaagtaa aattcaatcc ggaaaattat gtttctgagc aggtattcta tacatctgca gacggcacta aaattccaat gatgatcagt tataaaaaag gactgaaaaa agacgggaaa aatccaacaa tactatacag ctacggagga tttaatatca gtctacaacc tgcattctct gtggtaaatg ctatctggat ggaaaatggc ggaatctatg ctgttccgaa tatccgtggt ggcggtgaat acggaaaaaa atggcatgat gcaggaacca aacagcaaaa gaaaaatgtt tttaatgact ttatagctgc tggggaatat ttacagaaga acggttatac ttccaaagat tatatggcac tttcaggacg ttcgaatggg ggattgcttg ttggtgctac aatgacaatg cgcccggatc tagctaaagt agctttccct ggtgttggtg tactggatat gctgcgttat aataaattta cagcaggtgc cggatgggca tatgactacg gtacagccga ggatagtaaa gaaatgtttg agtatctgaa atcttattca cctgtacaca atgtaaaagc tggtacatgt tacccttcta caatggtaat tacgagtgat catgatgaca gagttgttcc tgcgcattca tttaaatttg gtgcagaatt acaggcaaaa caagcatgta agaacccggt tcttatccgt attgagacta atgcgggaca cggagcagga cgctctactg aacaagtagt gatggaaaat gcagatttgc tttcattctc attatatgaa atgggtatta aaaagctgaa gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for ElanA.18022.a.B2.PW38263
PURIFICATION DATe: 5/1/2017
CONCENTRATION: 29mg/ml
OBSERVED MW: 82kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHAQ NSNSLKYPET KKVNHTDTYF GNQVADPYRW LEDDRAEDTK AWVQQEVKFT QDYLAQIPFR EQIKKQLLDI WNYEKISAPF KKGKYTYFYK NDGLQAQSVL YRKDASGKTG VFLDPNKFSD KGTTSLANLS FNKKGTLVAY SISEGGSDWN KIIILDAETK KQIDETLIDV KFSGISWLGD EGFFYSSYDK PKDGSVLSGM TDKHKVYFHK LGTKQSQDEL IIGGDKFPRR YLSGYVTEDQ RYLVVSAANA TNGNELYIKD LKNKTDFIPI VTGFDSNVGL VDTDGDTLFL HTDKNAPNMR MVKTTIQNPK PETWKDVIAE TSEPMRVNSG GGYFFATYMK DALSQIKQYD KTGKLVREIK LPGNGTAGGF GGEKTEKELY YSFTNYITPP TIFKFNVDSG KSEVYQKPKV KFNPENYVSE QVFYTSADGT KIPMMISYKK GLKKDGKNPT ILYSYGGFNI SLQPAFSVVN AIWMENGGIY AVPNIRGGGE YGKKWHDAGT KQQKKNVFND FIAAGEYLQK NGYTSKDYMA LSGRSNGGLL VGATMTMRPD LAKVAFPGVG VLDMLRYNKF TAGAGWAYDY GTAEDSKEMF EYLKSYSPVH NVKAGTCYPS TMVITSDHDD RVVPAHSFKF GAELQAKQAC KNPVLIRIET NAGHGAGRST EQVVMENADL LSFSLYEMGI KKLK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCACAAA ATTCTAACTC ATTGAAGTAT CCCGAAACAA AGAAAGTAAA TCATACCGAT ACCTATTTTG GTAATCAGGT AGCAGACCCA TACCGTTGGC TGGAAGATGA CAGAGCTGAA GATACCAAAG CATGGGTACA GCAGGAGGTA AAATTTACGC AGGATTATCT TGCACAGATT CCTTTCCGCG AACAGATTAA AAAGCAACTT TTAGATATCT GGAATTATGA AAAGATTTCA GCTCCATTTA AAAAAGGTAA ATACACTTAT TTCTACAAAA ATGATGGACT GCAAGCACAG TCTGTGTTAT ACAGAAAAGA TGCTTCCGGA AAAACAGGAG TCTTTTTAGA TCCGAATAAA TTTTCGGATA AAGGAACAAC CTCTTTGGCT AATCTTTCAT TCAACAAAAA AGGAACACTA GTCGCTTACA GCATTTCTGA AGGAGGCTCT GACTGGAATA AGATTATCAT TCTGGATGCG GAAACTAAAA AACAGATCGA CGAGACACTT ATTGATGTAA AATTTAGTGG AATTTCCTGG TTAGGAGATG AAGGCTTCTT CTATTCCAGC TATGATAAAC CAAAAGACGG AAGTGTACTT TCCGGAATGA CGGATAAGCA CAAGGTATAT TTTCACAAGC TTGGAACAAA GCAGTCTCAG GATGAGCTGA TTATTGGAGG AGATAAATTC CCGAGAAGAT ATTTAAGTGG TTATGTGACA GAAGATCAGA GGTATCTTGT TGTTTCTGCA GCTAACGCAA CCAACGGGAA CGAACTCTAT ATTAAAGATC TTAAGAATAA AACAGATTTT ATCCCAATTG TTACAGGTTT TGATAGCAAT GTAGGTTTGG TTGATACAGA TGGAGATACA CTGTTCCTGC ATACTGATAA AAATGCGCCT AATATGCGCA TGGTAAAAAC AACAATTCAG AATCCGAAGC CGGAAACCTG GAAAGATGTT ATCGCTGAAA CATCTGAACC TATGCGTGTT AACTCAGGCG GAGGTTATTT CTTTGCGACT TACATGAAGG ATGCGCTAAG CCAGATTAAA CAATACGATA AAACCGGTAA GCTGGTTAGG GAAATCAAAC TGCCGGGTAA CGGTACTGCT GGTGGCTTTG GTGGTGAAAA AACAGAAAAA GAACTGTATT ATTCATTTAC CAACTACATT ACGCCTCCAA CGATCTTTAA ATTTAATGTA GATTCAGGAA AATCTGAGGT TTACCAAAAG CCAAAAGTAA AATTCAATCC GGAAAATTAT GTTTCTGAGC AGGTATTCTA TACATCTGCA GACGGCACTA AAATTCCAAT GATGATCAGT TATAAAAAAG GACTGAAAAA AGACGGGAAA AATCCAACAA TACTATACAG CTACGGAGGA TTTAATATCA GTCTACAACC TGCATTCTCT GTGGTAAATG CTATCTGGAT GGAAAATGGC GGAATCTATG CTGTTCCGAA TATCCGTGGT GGCGGTGAAT ACGGAAAAAA ATGGCATGAT GCAGGAACCA AACAGCAAAA GAAAAATGTT TTTAATGACT TTATAGCTGC TGGGGAATAT TTACAGAAGA ACGGTTATAC TTCCAAAGAT TATATGGCAC TTTCAGGACG TTCGAATGGG GGATTGCTTG TTGGTGCTAC AATGACAATG CGCCCGGATC TAGCTAAAGT AGCTTTCCCT GGTGTTGGTG TACTGGATAT GCTGCGTTAT AATAAATTTA CAGCAGGTGC CGGATGGGCA TATGACTACG GTACAGCCGA GGATAGTAAA GAAATGTTTG AGTATCTGAA ATCTTATTCA CCTGTACACA ATGTAAAAGC TGGTACATGT TACCCTTCTA CAATGGTAAT TACGAGTGAT CATGATGACA GAGTTGTTCC TGCGCATTCA TTTAAATTTG GTGCAGAATT ACAGGCAAAA CAAGCATGTA AGAACCCGGT TCTTATCCGT ATTGAGACTA ATGCGGGACA CGGAGCAGGA CGCTCTACTG AACAAGTAGT GATGGAAAAT GCAGATTTGC TTTCATTCTC ATTATATGAA ATGGGTATTA AAAAGCTGAA GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT