ElanA.18013.a

Elongation factor G

CENTER ID: ElanA.18013.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.18013.a.B1.GE40669 Full length(ElanA.18013.a) 1 705
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.18013.a.B1.PW38262 Full length(ElanA.18013.a) 1 705

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.2876
UniProt: A0A077EIY1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRDLKYTRN IGIAAHIDAG KTTTTERILF YTGKTHKIGE VHEGASTMDW MEQEAERGIT ITSAATTCSW NFPTDQGKSL PETKGYHFNI IDTPGHVDFT VEVNRSLRVL DGLVFLFSAV DGVEPQSETN WRLADNYKVA RMGFVNKMDR QGADFLNVVN QVKEMLGSNA VPIVLPIGAE EDFKGVVDLI KNRAIVWDEA GQGATFEVVP IPEDMKAEVL EYREKLVEAV ADYDETLMEK FFEDPDSISE DEINEALRKA TIDLSIIPMT CGSSFKNKGV QFMLDAVCKY LPSPMDKDDI KGTDPRTEEE ITRKPDVKEP FAALAFKIAT DPFVGRLAFF RAYSGRLDAG SYVLNTRSGN KERISRIYQM HANKQNPVEY IEAGDIGAAV GFKDIKTGDT LSDEKSPIVL ESMIFPDPVI GIAVEPKTKA DQDKLGNALA KLAEEDPTFQ VKTDEASGQT IISGMGELHL DIIVDRLRRE FKVEVNQGQP QVEYKESLTS TANHREVYKK QTGGRGKFAD IVFEIGPAED GKLGLEFINE IKGGNIPREF VPSVEKGFKE AMKNGPLAGF EIEGIKVTLK DGSFHPVDSD QLSFELAAKL GFKEAGRAAK PVIMEPIMKL EVVTPEEYMG DIVGDLNRRR GTVNGMDDRN NAKVIKAFVP LSEMFGYVTS LRTLSSGRAT SSMEFEKYEP APQNVAEDVI AKARG
NT Sequence
ATGAGTAGAG ATCTTAAATA CACAAGAAAT ATTGGTATCG CTGCACACAT CGATGCTGGT AAAACTACTA CAACTGAGCG TATCCTTTTC TATACAGGAA AAACTCACAA GATTGGTGAA GTTCACGAAG GTGCTTCTAC CATGGACTGG ATGGAGCAGG AAGCTGAAAG AGGTATTACC ATTACTTCTG CTGCTACAAC TTGTAGCTGG AACTTCCCTA CAGACCAAGG TAAATCTTTA CCAGAAACTA AAGGATACCA CTTCAATATC ATCGATACTC CGGGACACGT TGACTTCACT GTAGAGGTAA ACCGTTCTTT AAGAGTATTA GACGGGTTAG TATTCTTATT CTCTGCAGTA GACGGAGTAG AGCCTCAGTC AGAAACCAAC TGGAGACTTG CTGACAACTA CAAAGTTGCG AGAATGGGAT TCGTAAACAA AATGGACAGA CAAGGTGCTG ACTTCCTTAA CGTGGTGAAC CAGGTTAAAG AAATGTTAGG ATCTAACGCA GTTCCAATTG TATTACCTAT CGGTGCTGAA GAAGACTTCA AAGGTGTTGT AGACCTTATT AAGAACAGAG CAATCGTATG GGATGAGGCT GGACAAGGTG CTACTTTCGA AGTAGTTCCA ATTCCTGAGG ATATGAAAGC TGAAGTTCTT GAATACAGAG AGAAATTAGT AGAAGCAGTT GCTGACTACG ATGAAACTTT GATGGAGAAA TTCTTCGAAG ATCCAGATTC AATCTCTGAG GACGAAATCA ACGAAGCTTT AAGAAAAGCT ACAATTGATC TTTCTATTAT TCCAATGACT TGTGGATCAT CTTTCAAAAA TAAAGGTGTT CAGTTCATGC TAGATGCTGT ATGTAAATAT CTTCCTTCTC CAATGGATAA AGACGATATC AAAGGTACAG ATCCAAGAAC TGAAGAAGAA ATTACAAGAA AACCAGACGT TAAAGAACCA TTCGCAGCTT TAGCATTTAA AATTGCTACT GACCCATTCG TAGGTCGTTT AGCATTCTTC AGAGCTTACT CTGGTAGACT AGATGCAGGT TCTTATGTTC TTAACACAAG ATCTGGTAAC AAAGAAAGAA TTTCAAGAAT CTATCAGATG CACGCTAACA AGCAAAACCC TGTTGAGTAT ATCGAGGCAG GAGATATCGG TGCAGCTGTA GGTTTCAAGG ATATTAAAAC TGGTGATACC CTATCTGATG AAAAGAGCCC TATCGTTCTT GAATCTATGA TCTTCCCAGA TCCGGTAATC GGTATCGCTG TTGAGCCTAA AACTAAAGCA GACCAGGATA AATTGGGTAA CGCATTAGCT AAGCTTGCTG AAGAAGATCC AACTTTCCAG GTTAAAACTG ATGAGGCTTC TGGCCAAACA ATTATCTCTG GTATGGGTGA GCTTCACTTA GATATCATTG TAGACCGTTT AAGAAGAGAA TTCAAAGTTG AAGTTAACCA AGGTCAGCCT CAGGTTGAGT ACAAAGAATC TCTTACCTCT ACTGCTAACC ACAGAGAAGT TTACAAAAAA CAAACTGGCG GTCGTGGTAA ATTCGCAGAT ATCGTATTTG AAATCGGTCC TGCAGAAGAT GGTAAGTTAG GTCTTGAGTT CATCAACGAA ATTAAAGGTG GTAACATTCC AAGAGAATTT GTTCCTTCTG TAGAAAAAGG TTTCAAAGAG GCTATGAAAA ATGGTCCTTT AGCTGGATTC GAAATTGAAG GTATTAAAGT AACTCTTAAG GATGGATCTT TCCACCCGGT TGACTCCGAT CAGTTATCTT TCGAATTAGC TGCTAAGCTA GGTTTCAAAG AAGCTGGTAG AGCTGCTAAG CCTGTAATCA TGGAACCTAT TATGAAACTT GAAGTTGTAA CTCCTGAGGA GTACATGGGG GACATCGTAG GTGACCTTAA CAGAAGAAGA GGTACAGTAA ACGGTATGGA TGACAGAAAT AACGCTAAAG TTATCAAAGC ATTCGTTCCA TTATCAGAAA TGTTCGGTTA TGTAACTTCA TTAAGAACAT TATCTTCTGG TAGAGCTACA TCTTCTATGG AATTTGAGAA ATACGAACCA GCTCCGCAAA ACGTTGCTGA GGATGTAATC GCAAAAGCAA GAGGT
Details for ElanA.18013.a.B1.GE40669
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 79kDa
OBSERVED MW: 79kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS RDLKYTRNIG IAAHIDAGKT TTTERILFYT GKTHKIGEVH EGASTMDWME QEAERGITIT SAATTCSWNF PTDQGKSLPE TKGYHFNIID TPGHVDFTVE VNRSLRVLDG LVFLFSAVDG VEPQSETNWR LADNYKVARM GFVNKMDRQG ADFLNVVNQV KEMLGSNAVP IVLPIGAEED FKGVVDLIKN RAIVWDEAGQ GATFEVVPIP EDMKAEVLEY REKLVEAVAD YDETLMEKFF EDPDSISEDE INEALRKATI DLSIIPMTCG SSFKNKGVQF MLDAVCKYLP SPMDKDDIKG TDPRTEEEIT RKPDVKEPFA ALAFKIATDP FVGRLAFFRA YSGRLDAGSY VLNTRSGNKE RISRIYQMHA NKQNPVEYIE AGDIGAAVGF KDIKTGDTLS DEKSPIVLES MIFPDPVIGI AVEPKTKADQ DKLGNALAKL AEEDPTFQVK TDEASGQTII SGMGELHLDI IVDRLRREFK VEVNQGQPQV EYKESLTSTA NHREVYKKQT GGRGKFADIV FEIGPAEDGK LGLEFINEIK GGNIPREFVP SVEKGFKEAM KNGPLAGFEI EGIKVTLKDG SFHPVDSDQL SFELAAKLGF KEAGRAAKPV IMEPIMKLEV VTPEEYMGDI VGDLNRRRGT VNGMDDRNNA KVIKAFVPLS EMFGYVTSLR TLSSGRATSS MEFEKYEPAP QNVAEDVIAK ARG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta gagatcttaa atacacaaga aatattggta tcgctgcaca catcgatgct ggtaaaacta ctacaactga gcgtatcctt ttctatacag gaaaaactca caagattggt gaagttcacg aaggtgcttc taccatggac tggatggagc aggaagctga aagaggtatt accattactt ctgctgctac aacttgtagc tggaacttcc ctacagacca aggtaaatct ttaccagaaa ctaaaggata ccacttcaat atcatcgata ctccgggaca cgttgacttc actgtagagg taaaccgttc tttaagagta ttagacgggt tagtattctt attctctgca gtagacggag tagagcctca gtcagaaacc aactggagac ttgctgacaa ctacaaagtt gcgagaatgg gattcgtaaa caaaatggac agacaaggtg ctgacttcct taacgtggtg aaccaggtta aagaaatgtt aggatctaac gcagttccaa ttgtattacc tatcggtgct gaagaagact tcaaaggtgt tgtagacctt attaagaaca gagcaatcgt atgggatgag gctggacaag gtgctacttt cgaagtagtt ccaattcctg aggatatgaa agctgaagtt cttgaataca gagagaaatt agtagaagca gttgctgact acgatgaaac tttgatggag aaattcttcg aagatccaga ttcaatctct gaggacgaaa tcaacgaagc tttaagaaaa gctacaattg atctttctat tattccaatg acttgtggat catctttcaa aaataaaggt gttcagttca tgctagatgc tgtatgtaaa tatcttcctt ctccaatgga taaagacgat atcaaaggta cagatccaag aactgaagaa gaaattacaa gaaaaccaga cgttaaagaa ccattcgcag ctttagcatt taaaattgct actgacccat tcgtaggtcg tttagcattc ttcagagctt actctggtag actagatgca ggttcttatg ttcttaacac aagatctggt aacaaagaaa gaatttcaag aatctatcag atgcacgcta acaagcaaaa ccctgttgag tatatcgagg caggagatat cggtgcagct gtaggtttca aggatattaa aactggtgat accctatctg atgaaaagag ccctatcgtt cttgaatcta tgatcttccc agatccggta atcggtatcg ctgttgagcc taaaactaaa gcagaccagg ataaattggg taacgcatta gctaagcttg ctgaagaaga tccaactttc caggttaaaa ctgatgaggc ttctggccaa acaattatct ctggtatggg tgagcttcac ttagatatca ttgtagaccg tttaagaaga gaattcaaag ttgaagttaa ccaaggtcag cctcaggttg agtacaaaga atctcttacc tctactgcta accacagaga agtttacaaa aaacaaactg gcggtcgtgg taaattcgca gatatcgtat ttgaaatcgg tcctgcagaa gatggtaagt taggtcttga gttcatcaac gaaattaaag gtggtaacat tccaagagaa tttgttcctt ctgtagaaaa aggtttcaaa gaggctatga aaaatggtcc tttagctgga ttcgaaattg aaggtattaa agtaactctt aaggatggat ctttccaccc ggttgactcc gatcagttat ctttcgaatt agctgctaag ctaggtttca aagaagctgg tagagctgct aagcctgtaa tcatggaacc tattatgaaa cttgaagttg taactcctga ggagtacatg ggggacatcg taggtgacct taacagaaga agaggtacag taaacggtat ggatgacaga aataacgcta aagttatcaa agcattcgtt ccattatcag aaatgttcgg ttatgtaact tcattaagaa cattatcttc tggtagagct acatcttcta tggaatttga gaaatacgaa ccagctccgc aaaacgttgc tgaggatgta atcgcaaaag caagaggttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for ElanA.18013.a.B1.PW38262
PURIFICATION DATe: 5/1/2017
CONCENTRATION: 31.5mg/ml
OBSERVED MW: 85kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS RDLKYTRNIG IAAHIDAGKT TTTERILFYT GKTHKIGEVH EGASTMDWME QEAERGITIT SAATTCSWNF PTDQGKSLPE TKGYHFNIID TPGHVDFTVE VNRSLRVLDG LVFLFSAVDG VEPQSETNWR LADNYKVARM GFVNKMDRQG ADFLNVVNQV KEMLGSNAVP IVLPIGAEED FKGVVDLIKN RAIVWDEAGQ GATFEVVPIP EDMKAEVLEY REKLVEAVAD YDETLMEKFF EDPDSISEDE INEALRKATI DLSIIPMTCG SSFKNKGVQF MLDAVCKYLP SPMDKDDIKG TDPRTEEEIT RKPDVKEPFA ALAFKIATDP FVGRLAFFRA YSGRLDAGSY VLNTRSGNKE RISRIYQMHA NKQNPVEYIE AGDIGAAVGF KDIKTGDTLS DEKSPIVLES MIFPDPVIGI AVEPKTKADQ DKLGNALAKL AEEDPTFQVK TDEASGQTII SGMGELHLDI IVDRLRREFK VEVNQGQPQV EYKESLTSTA NHREVYKKQT GGRGKFADIV FEIGPAEDGK LGLEFINEIK GGNIPREFVP SVEKGFKEAM KNGPLAGFEI EGIKVTLKDG SFHPVDSDQL SFELAAKLGF KEAGRAAKPV IMEPIMKLEV VTPEEYMGDI VGDLNRRRGT VNGMDDRNNA KVIKAFVPLS EMFGYVTSLR TLSSGRATSS MEFEKYEPAP QNVAEDVIAK ARG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTA GAGATCTTAA ATACACAAGA AATATTGGTA TCGCTGCACA CATCGATGCT GGTAAAACTA CTACAACTGA GCGTATCCTT TTCTATACAG GAAAAACTCA CAAGATTGGT GAAGTTCACG AAGGTGCTTC TACCATGGAC TGGATGGAGC AGGAAGCTGA AAGAGGTATT ACCATTACTT CTGCTGCTAC AACTTGTAGC TGGAACTTCC CTACAGACCA AGGTAAATCT TTACCAGAAA CTAAAGGATA CCACTTCAAT ATCATCGATA CTCCGGGACA CGTTGACTTC ACTGTAGAGG TAAACCGTTC TTTAAGAGTA TTAGACGGGT TAGTATTCTT ATTCTCTGCA GTAGACGGAG TAGAGCCTCA GTCAGAAACC AACTGGAGAC TTGCTGACAA CTACAAAGTT GCGAGAATGG GATTCGTAAA CAAAATGGAC AGACAAGGTG CTGACTTCCT TAACGTGGTG AACCAGGTTA AAGAAATGTT AGGATCTAAC GCAGTTCCAA TTGTATTACC TATCGGTGCT GAAGAAGACT TCAAAGGTGT TGTAGACCTT ATTAAGAACA GAGCAATCGT ATGGGATGAG GCTGGACAAG GTGCTACTTT CGAAGTAGTT CCAATTCCTG AGGATATGAA AGCTGAAGTT CTTGAATACA GAGAGAAATT AGTAGAAGCA GTTGCTGACT ACGATGAAAC TTTGATGGAG AAATTCTTCG AAGATCCAGA TTCAATCTCT GAGGACGAAA TCAACGAAGC TTTAAGAAAA GCTACAATTG ATCTTTCTAT TATTCCAATG ACTTGTGGAT CATCTTTCAA AAATAAAGGT GTTCAGTTCA TGCTAGATGC TGTATGTAAA TATCTTCCTT CTCCAATGGA TAAAGACGAT ATCAAAGGTA CAGATCCAAG AACTGAAGAA GAAATTACAA GAAAACCAGA CGTTAAAGAA CCATTCGCAG CTTTAGCATT TAAAATTGCT ACTGACCCAT TCGTAGGTCG TTTAGCATTC TTCAGAGCTT ACTCTGGTAG ACTAGATGCA GGTTCTTATG TTCTTAACAC AAGATCTGGT AACAAAGAAA GAATTTCAAG AATCTATCAG ATGCACGCTA ACAAGCAAAA CCCTGTTGAG TATATCGAGG CAGGAGATAT CGGTGCAGCT GTAGGTTTCA AGGATATTAA AACTGGTGAT ACCCTATCTG ATGAAAAGAG CCCTATCGTT CTTGAATCTA TGATCTTCCC AGATCCGGTA ATCGGTATCG CTGTTGAGCC TAAAACTAAA GCAGACCAGG ATAAATTGGG TAACGCATTA GCTAAGCTTG CTGAAGAAGA TCCAACTTTC CAGGTTAAAA CTGATGAGGC TTCTGGCCAA ACAATTATCT CTGGTATGGG TGAGCTTCAC TTAGATATCA TTGTAGACCG TTTAAGAAGA GAATTCAAAG TTGAAGTTAA CCAAGGTCAG CCTCAGGTTG AGTACAAAGA ATCTCTTACC TCTACTGCTA ACCACAGAGA AGTTTACAAA AAACAAACTG GCGGTCGTGG TAAATTCGCA GATATCGTAT TTGAAATCGG TCCTGCAGAA GATGGTAAGT TAGGTCTTGA GTTCATCAAC GAAATTAAAG GTGGTAACAT TCCAAGAGAA TTTGTTCCTT CTGTAGAAAA AGGTTTCAAA GAGGCTATGA AAAATGGTCC TTTAGCTGGA TTCGAAATTG AAGGTATTAA AGTAACTCTT AAGGATGGAT CTTTCCACCC GGTTGACTCC GATCAGTTAT CTTTCGAATT AGCTGCTAAG CTAGGTTTCA AAGAAGCTGG TAGAGCTGCT AAGCCTGTAA TCATGGAACC TATTATGAAA CTTGAAGTTG TAACTCCTGA GGAGTACATG GGGGACATCG TAGGTGACCT TAACAGAAGA AGAGGTACAG TAAACGGTAT GGATGACAGA AATAACGCTA AAGTTATCAA AGCATTCGTT CCATTATCAG AAATGTTCGG TTATGTAACT TCATTAAGAA CATTATCTTC TGGTAGAGCT ACATCTTCTA TGGAATTTGA GAAATACGAA CCAGCTCCGC AAAACGTTGC TGAGGATGTA ATCGCAAAAG CAAGAGGTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT