ElanA.17987.a
DNA polymerase III subunit beta (EC 2.7.7.7)
| CENTER ID: | ElanA.17987.a |
| ORGANISM: | Elizabethkingia anophelis NUHP1 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ElanA.17987.a.AE1.GE44056 | full length | 1 | 376 | ||
| ElanA.17987.a.B1.GE41092 | full length | 1 | 376 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ElanA.17987.a.AE1.PS38659 | full length | 1 | 376 |
Structures
8DT6
DEPOSITED: 7/25/2022
DETERMINATION: XRay
CLONE: ElanA.17987.a.AE1.GE44056
PROTEIN: ElanA.17987.a.AE1.PS38659
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1338011.4.peg.1991 |
| UniProt: | A0A077EHW1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFIVASGEL QKALQTVSGV ISGSQSRPIL ENFLFELEND NLKITASDGE TTLITSIPVK SENNGRMAVP AKMFLDVIKS FGDQPLTFVE KESENGIGSL LEILDEKDNY FVALDNAEDY PELPEFDASK SVKIQAGILS EALVNTLFAT SNDSLRPVMT GVLFQFNENE ANFVSTDSHR LVVYNRKDVM NVDNIEFIMP KKPLAIIKNI LSNTDDEVLI EFNENMAKFS FQDNIWICRL IDGKYPNYTA VIPKENPNVL TINRNLLLSS IRRASIFSNK STNQVRFKLS GNLLHLHAED TEYANKADMQ IPCEYNGEDI NIGFSSKFLT EMLSVLGSDD ITMKMSQPNR PGIIEPVDGL EENESILMLS MPVIGL
NT Sequence
atgaaattta ttgttgccag cggcgagtta caaaaagcct tgcagacggt aagcggagta atttctggtt cccagtcaag accgatttta gagaattttc tttttgaact ggaaaatgat aatctaaaaa ttactgcttc cgacggtgaa acaactttaa taacttctat tcctgtaaag tctgaaaaca acgggagaat ggccgttccg gctaaaatgt ttctggatgt aattaaatca ttcggagatc agcctcttac ttttgtagaa aaagaatctg aaaacggaat tggcagtctt cttgagattt tagacgagaa ggataattac tttgtagcat tagataatgc tgaggattat cctgaattac cagaattcga tgcctctaaa agcgtgaaaa ttcaggcagg aatattgtca gaagcattgg ttaacactct gtttgcaaca agtaacgact ctctaagacc tgtaatgaca ggtgttttgt tccagtttaa tgagaacgaa gctaactttg tgtctaccga ctctcaccgt ttggtagtat acaacagaaa agatgtaatg aatgttgata atattgaatt cattatgccg aagaagcctt tggctatcat caagaatatt ctgtctaata cagatgatga agttcttatc gagttcaatg aaaatatggc taagttcagc ttccaggata atatctggat ctgtcgtctt atcgatggta aatatcctaa ttatacagct gttatcccga aagagaatcc taacgtacta acgattaaca gaaacttact actaagttct atcagaagag catctatctt ctctaataaa tcgactaatc aagtaagatt caagctttca ggaaatttac ttcatcttca tgctgaagat actgaatatg caaataaagc agatatgcag attccgtgtg agtataatgg agaagatatt aatatcggat tcagctccaa gtttttaaca gaaatgcttt ctgttttagg atcagatgat attacgatga agatgtcaca accaaaccgt ccgggaataa tagaaccggt agacggtctt gaagaaaacg agagcatcct tatgctttcg atgcctgtaa ttggcctata a
Details for ElanA.17987.a.AE1.GE44056
| HARVESTED ON: | 1/7/2021 |
| SEQUENCED ON: | 1/8/2021 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 19 |
| PERCENT COVERAGE: | 33 |
Validated AA Sequence
MIAKGFXGIX NXILSXFITS FLLXTTXXXX SXHXKLASXS SNXXXHPVXT XLQSRYLXXX VXTNASDIXL XEVCXXEXXR ILXTXDSPXI IXXXXSXYPS SSXISXXCXX RFXXXXXXKX XADRPMX
Validated NT Sequence
cnnnnnnnnn nnnnnngtta gcngccggat ctcnntggng nnggtggtgg tgctcgagtg cggccgcaag cntgncnacg gagctcgaat tcggatccgt taatgatgat gatggtggtg cccagctagg ccaattacag gcatcgaaag cataaggatg ctctcgtttt cttcaagacc gtctaccgnt tctattattg ccggacggtt tggttgtgac atcttcgtcg naatatcatc tgatcctaaa agagaaagct tttctgttaa aaacttggag ctgaatccga tattaatatc ttctccncta tactcacacg gaatctgcat atctgcttta tgtgcttatt gnggatcttc ngcatgaaga tgaaataaat ttcctganag cttgaatctt acttgattag tcgatttatt agagaagata gatgctcttc tgatagaact tagtactaaa tttctgataa tcgctagacg tnangattct ctctccggat aacagctgta taattacgat atttaccatc gaaaagacga cagattcaga tattatcctg gaagctgaac ttaaccatat tttcattgaa ctcgataaga acttnntcnt ctgtattana cnnaatattc ttgatgatag ccaaaggctt cntcggcata ntgaattnna tattatcnnc attcattaca tcttttctgt tgnatactac canncngngn nantcnnnac acncnaagct agcttcnntc tcntcaaact gnancanaca tcctgtcatn acangcttac agagtcgtta cttgnngcna cnagtgntna ccaatgcttc tgacatantc ctgnctgaag tntgccnnnt tgagannnnt cgaattctgg naactcngga tagtccncnc attatcnanc nnntacnnag tnattatcct tcctcgtcta naatctcaga nnactgccan tnccgtttcn nantcttntt ncnacnaaag tannaggcng atcgtccgat ganttagnta cnntccaaan catttngccg nacnncnnnt cccgtngttt ncanannnnn nngantanaa antataangg tgggttcc
Expected Protein Sequence
MKFIVASGEL QKALQTVSGV ISGSQSRPIL ENFLFELEND NLKITASDGE TTLITSIPVK SENNGRMAVP AKMFLDVIKS FGDQPLTFVE KESENGIGSL LEILDEKDNY FVALDNAEDY PELPEFDASK SVKIQAGILS EALVNTLFAT SNDSLRPVMT GVLFQFNENE ANFVSTDSHR LVVYNRKDVM NVDNIEFIMP KKPLAIIKNI LSNTDDEVLI EFNENMAKFS FQDNIWICRL IDGKYPNYTA VIPKENPNVL TINRNLLLSS IRRASIFSNK STNQVRFKLS GNLLHLHAED TEYANKADMQ IPCEYNGEDI NIGFSSKFLT EMLSVLGSDD ITMKMSQPNR PGIIEPVDGL EENESILMLS MPVIGLGHHH HHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gaaatttatt gttgccagcg gcgagttaca aaaagccttg cagacggtaa gcggagtaat ttctggttcc cagtcaagac cgattttaga gaattttctt tttgaactgg aaaatgataa tctaaaaatt actgcttccg acggtgaaac aactttaata acttctattc ctgtaaagtc tgaaaacaac gggagaatgg ccgttccggc taaaatgttt ctggatgtaa ttaaatcatt cggagatcag cctcttactt ttgtagaaaa agaatctgaa aacggaattg gcagtcttct tgagatttta gacgagaagg ataattactt tgtagcatta gataatgctg aggattatcc tgaattacca gaattcgatg cctctaaaag cgtgaaaatt caggcaggaa tattgtcaga agcattggtt aacactctgt ttgcaacaag taacgactct ctaagacctg taatgacagg tgttttgttc cagtttaatg agaacgaagc taactttgtg tctaccgact ctcaccgttt ggtagtatac aacagaaaag atgtaatgaa tgttgataat attgaattca ttatgccgaa gaagcctttg gctatcatca agaatattct gtctaataca gatgatgaag ttcttatcga gttcaatgaa aatatggcta agttcagctt ccaggataat atctggatct gtcgtcttat cgatggtaaa tatcctaatt atacagctgt tatcccgaaa gagaatccta acgtactaac gattaacaga aacttactac taagttctat cagaagagca tctatcttct ctaataaatc gactaatcaa gtaagattca agctttcagg aaatttactt catcttcatg ctgaagatac tgaatatgca aataaagcag atatgcagat tccgtgtgag tataatggag aagatattaa tatcggattc agctccaagt ttttaacaga aatgctttct gttttaggat cagatgatat tacgatgaag atgtcacaac caaaccgtcc gggaataata gaaccggtag acggtcttga agaaaacgag agcatcctta tgctttcgat gcctgtaatt ggcctagggc accaccatca tcatcattaa cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggat
Details for ElanA.17987.a.B1.GE41092
| HARVESTED ON: | 2/8/2017 |
| SEQUENCED ON: | 2/13/2017 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | No Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMK FIVASGELQK ALQTVSGVIS GSQSRPILEN FLFELENDNL KITASDGETT LITSIPVKSE NNGRMAVPAK MFLDVIKSFG DQPLTFVEKE SENGIGSLLE ILDEKDNYFV ALDNAEDYPE LPEFDASKSV KIQAGILSEA LVNTLFATSN DSLRPVMTGV LFQFNENEAN FVSTDSHRLV VYNRKDVMNV DNIEFIMPKK PLAIIKNILS NTDDEVLIEF NENMAKFSFQ DNIWICRLID GKYPNYTAVI PKENPNVLTI NRNLLLSSIR RASIFSNKST NQVRFKLSGN LLHLHAEDTE YANKADMQIP CEYNGEDINI GFSSKFLTEM LSVLGSDDIT MKMSQPNRPG IIEPVDGLEE NESIL
Validated NT Sequence
ataaggatgc tctcgttttc ttcaagaccg tctaccggtt ctattattcc cggacggttt ggttgtgaca tcttcatcgt aatatcatct gatcctaaaa cagaaagcat ttctgttaaa aacttggagc tgaatccgat attaatatct tctccattat actcacacgg aatctgcata tctgctttat ttgcatattc agtatcttca gcatgaagat gaagtaaatt tcctgaaagc ttgaatctta cttgattagt cgatttatta gagaagatag atgctcttct gatagaactt agtagtaagt ttctgttaat cgttagtacg ttaggattct ctttcgggat aacagctgta taattaggat atttaccatc gataagacga cagatccaga tattatcctg gaagctgaac ttagccatat tttcattgaa ctcgataaga acttcatcat ctgtattaga cagaatattc ttgatgatag ccaaaggctt cttcggcata atgaattcaa tattatcaac attcattaca tcttttctgt tgtatactac caaacggtga gagtcggtag acacaaagtt agcttcgttc tcattaaact ggaacaaaac acctgtcatt acaggtctta gagagtcgtt acttgttgca aacagagtgt taaccaatgc ttctgacaat attcctgcct gaattttcac gcttttagag gcatcgaatt ctggtaattc aggataatcc tcagcattat ctaatgctac aaagtaatta tccttctcgt ctaaaatctc aagaagactg ccaattccgt tttcagattc tttttctaca aaagtaagag gctgatctcc gaatgattta attacatcca gaaacatttt agccggaacg gccattctcc cgttgttttc agactttaca ggaatagaag ttattaaagt tgtttcaccg tcggaagcag taatttttag attatcattt tccagttcaa aaagaaaatt ctctaaaatc ggtcttgact gggaaccaga aattactccg cttaccgtct gcaaggcttt ttgtaactcg ccgctggcaa caataaattt catatggtgg tggtggtggt gagccatggt atatc
Expected Protein Sequence
MAHHHHHHMK FIVASGELQK ALQTVSGVIS GSQSRPILEN FLFELENDNL KITASDGETT LITSIPVKSE NNGRMAVPAK MFLDVIKSFG DQPLTFVEKE SENGIGSLLE ILDEKDNYFV ALDNAEDYPE LPEFDASKSV KIQAGILSEA LVNTLFATSN DSLRPVMTGV LFQFNENEAN FVSTDSHRLV VYNRKDVMNV DNIEFIMPKK PLAIIKNILS NTDDEVLIEF NENMAKFSFQ DNIWICRLID GKYPNYTAVI PKENPNVLTI NRNLLLSSIR RASIFSNKST NQVRFKLSGN LLHLHAEDTE YANKADMQIP CEYNGEDINI GFSSKFLTEM LSVLGSDDIT MKMSQPNRPG IIEPVDGLEE NESILMLSMP VIGL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat ttattgttgc cagcggcgag ttacaaaaag ccttgcagac ggtaagcgga gtaatttctg gttcccagtc aagaccgatt ttagagaatt ttctttttga actggaaaat gataatctaa aaattactgc ttccgacggt gaaacaactt taataacttc tattcctgta aagtctgaaa acaacgggag aatggccgtt ccggctaaaa tgtttctgga tgtaattaaa tcattcggag atcagcctct tacttttgta gaaaaagaat ctgaaaacgg aattggcagt cttcttgaga ttttagacga gaaggataat tactttgtag cattagataa tgctgaggat tatcctgaat taccagaatt cgatgcctct aaaagcgtga aaattcaggc aggaatattg tcagaagcat tggttaacac tctgtttgca acaagtaacg actctctaag acctgtaatg acaggtgttt tgttccagtt taatgagaac gaagctaact ttgtgtctac cgactctcac cgtttggtag tatacaacag aaaagatgta atgaatgttg ataatattga attcattatg ccgaagaagc ctttggctat catcaagaat attctgtcta atacagatga tgaagttctt atcgagttca atgaaaatat ggctaagttc agcttccagg ataatatctg gatctgtcgt cttatcgatg gtaaatatcc taattataca gctgttatcc cgaaagagaa tcctaacgta ctaacgatta acagaaactt actactaagt tctatcagaa gagcatctat cttctctaat aaatcgacta atcaagtaag attcaagctt tcaggaaatt tacttcatct tcatgctgaa gatactgaat atgcaaataa agcagatatg cagattccgt gtgagtataa tggagaagat attaatatcg gattcagctc caagttttta acagaaatgc tttctgtttt aggatcagat gatattacga tgaagatgtc acaaccaaac cgtccgggaa taatagaacc ggtagacggt cttgaagaaa acgagagcat ccttatgctt tcgatgcctg taattggcct atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for ElanA.17987.a.AE1.PS38659
| PURIFICATION DATe: | 2/9/2021 |
| CONCENTRATION: | 28.82mg/ml |
| OBSERVED MW: | 44kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 19 |
| PERCENT COVERAGE: | 33 |
Protocol Notes
notes unavailable
Validated AA Sequence
MIAKGFXGIX NXILSXFITS FLLXTTXXXX SXHXKLASXS SNXXXHPVXT XLQSRYLXXX VXTNASDIXL XEVCXXEXXR ILXTXDSPXI IXXXXSXYPS SSXISXXCXX RFXXXXXXKX XADRPMX
Validated NT Sequence
cnnnnnnnnn nnnnnngtta gcngccggat ctcnntggng nnggtggtgg tgctcgagtg cggccgcaag cntgncnacg gagctcgaat tcggatccgt taatgatgat gatggtggtg cccagctagg ccaattacag gcatcgaaag cataaggatg ctctcgtttt cttcaagacc gtctaccgnt tctattattg ccggacggtt tggttgtgac atcttcgtcg naatatcatc tgatcctaaa agagaaagct tttctgttaa aaacttggag ctgaatccga tattaatatc ttctccncta tactcacacg gaatctgcat atctgcttta tgtgcttatt gnggatcttc ngcatgaaga tgaaataaat ttcctganag cttgaatctt acttgattag tcgatttatt agagaagata gatgctcttc tgatagaact tagtactaaa tttctgataa tcgctagacg tnangattct ctctccggat aacagctgta taattacgat atttaccatc gaaaagacga cagattcaga tattatcctg gaagctgaac ttaaccatat tttcattgaa ctcgataaga acttnntcnt ctgtattana cnnaatattc ttgatgatag ccaaaggctt cntcggcata ntgaattnna tattatcnnc attcattaca tcttttctgt tgnatactac canncngngn nantcnnnac acncnaagct agcttcnntc tcntcaaact gnancanaca tcctgtcatn acangcttac agagtcgtta cttgnngcna cnagtgntna ccaatgcttc tgacatantc ctgnctgaag tntgccnnnt tgagannnnt cgaattctgg naactcngga tagtccncnc attatcnanc nnntacnnag tnattatcct tcctcgtcta naatctcaga nnactgccan tnccgtttcn nantcttntt ncnacnaaag tannaggcng atcgtccgat ganttagnta cnntccaaan catttngccg nacnncnnnt cccgtngttt ncanannnnn nngantanaa antataangg tgggttcc
Expressed Protein Sequence
MKFIVASGEL QKALQTVSGV ISGSQSRPIL ENFLFELEND NLKITASDGE TTLITSIPVK SENNGRMAVP AKMFLDVIKS FGDQPLTFVE KESENGIGSL LEILDEKDNY FVALDNAEDY PELPEFDASK SVKIQAGILS EALVNTLFAT SNDSLRPVMT GVLFQFNENE ANFVSTDSHR LVVYNRKDVM NVDNIEFIMP KKPLAIIKNI LSNTDDEVLI EFNENMAKFS FQDNIWICRL IDGKYPNYTA VIPKENPNVL TINRNLLLSS IRRASIFSNK STNQVRFKLS GNLLHLHAED TEYANKADMQ IPCEYNGEDI NIGFSSKFLT EMLSVLGSDD ITMKMSQPNR PGIIEPVDGL EENESILMLS MPVIGLGHHH HHH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GAAATTTATT GTTGCCAGCG GCGAGTTACA AAAAGCCTTG CAGACGGTAA GCGGAGTAAT TTCTGGTTCC CAGTCAAGAC CGATTTTAGA GAATTTTCTT TTTGAACTGG AAAATGATAA TCTAAAAATT ACTGCTTCCG ACGGTGAAAC AACTTTAATA ACTTCTATTC CTGTAAAGTC TGAAAACAAC GGGAGAATGG CCGTTCCGGC TAAAATGTTT CTGGATGTAA TTAAATCATT CGGAGATCAG CCTCTTACTT TTGTAGAAAA AGAATCTGAA AACGGAATTG GCAGTCTTCT TGAGATTTTA GACGAGAAGG ATAATTACTT TGTAGCATTA GATAATGCTG AGGATTATCC TGAATTACCA GAATTCGATG CCTCTAAAAG CGTGAAAATT CAGGCAGGAA TATTGTCAGA AGCATTGGTT AACACTCTGT TTGCAACAAG TAACGACTCT CTAAGACCTG TAATGACAGG TGTTTTGTTC CAGTTTAATG AGAACGAAGC TAACTTTGTG TCTACCGACT CTCACCGTTT GGTAGTATAC AACAGAAAAG ATGTAATGAA TGTTGATAAT ATTGAATTCA TTATGCCGAA GAAGCCTTTG GCTATCATCA AGAATATTCT GTCTAATACA GATGATGAAG TTCTTATCGA GTTCAATGAA AATATGGCTA AGTTCAGCTT CCAGGATAAT ATCTGGATCT GTCGTCTTAT CGATGGTAAA TATCCTAATT ATACAGCTGT TATCCCGAAA GAGAATCCTA ACGTACTAAC GATTAACAGA AACTTACTAC TAAGTTCTAT CAGAAGAGCA TCTATCTTCT CTAATAAATC GACTAATCAA GTAAGATTCA AGCTTTCAGG AAATTTACTT CATCTTCATG CTGAAGATAC TGAATATGCA AATAAAGCAG ATATGCAGAT TCCGTGTGAG TATAATGGAG AAGATATTAA TATCGGATTC AGCTCCAAGT TTTTAACAGA AATGCTTTCT GTTTTAGGAT CAGATGATAT TACGATGAAG ATGTCACAAC CAAACCGTCC GGGAATAATA GAACCGGTAG ACGGTCTTGA AGAAAACGAG AGCATCCTTA TGCTTTCGAT GCCTGTAATT GGCCTAGGGC ACCACCATCA TCATCATTAA CGGATCCGAA TTCGAGCTCC GTCGACAAGC TTGCGGCCGC ACTCGAGCAC CACCACCACC ACCACTGAGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGAT