SSGCID: Target Page

ElanA.17876.a

GMP synthase [glutamine-hydrolyzing]

CENTER ID:	ElanA.17876.a
ORGANISM:	Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.17876.a.B1.GE40668	Full length(ElanA.17876.a)		1	509

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.17876.a.B1.PW38261	Full length(ElanA.17876.a)		1	509

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1338011.4.peg.948
UniProt:	A0A077EGS9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MERGIIILDF GSQYNQLIGR RVREMGVYSE IIPYYTPLEE IKERNPFGII LSGGPSSVNA EDAHLVSKEI FELGIPVLGI CYGMQLTAHL LGGKVAKGVK GEYGKAELEI VKACSLFDGV KERSIAWMSH FDEVETAPQG FDIVAKSGVI AGMANEDKKI FTVQFHPEVS HTEEGSKMLE NFVLNICQSE RSWKLTNYIE RTVAEIKEKV GSQKVILGLS GGVDSSVAAV LIHKAIGDQL TCIFVDTGLL RKDEAKKVME NYGQHFHMNI KLVDAADRFL SKLAGVDDPE QKRKIIGNEF IAVFDEESHK IEGAKFLAQG TIYPDVIESQ SVKGPSAVIK SHHNVGGLPE EMEFELLEPL RELFKDEVRK VGEELGIPHH LVYRHPFPGP GLGIRVLGAV DVEKVRILQE ADDIFIEELY KNDLYEKVSQ AFVVLLPVKS VGVMGDERTY EYTAVVRSAN TIDFMTATWS KLPYEFLETV SNRIINEVRG INRVAYDISS KPPATIEWE

NT Sequence

ATGGAAAGAG GAATTATTAT TTTAGACTTC GGTTCACAAT ACAACCAGTT GATCGGAAGA AGAGTAAGAG AGATGGGAGT ATATTCTGAA ATTATCCCTT ACTACACACC ATTGGAAGAA ATTAAAGAAA GAAATCCTTT CGGTATTATT CTTTCAGGAG GACCTTCTTC TGTAAATGCA GAAGATGCAC ATTTGGTTTC AAAAGAAATT TTCGAATTAG GAATTCCTGT TTTAGGGATT TGCTATGGAA TGCAATTAAC AGCTCACCTT TTGGGAGGTA AAGTTGCTAA AGGTGTAAAA GGAGAATATG GAAAAGCTGA ATTGGAGATC GTAAAAGCAT GTTCTTTATT CGACGGAGTA AAAGAAAGAT CTATAGCATG GATGAGCCAC TTCGACGAAG TAGAAACTGC TCCTCAGGGA TTCGATATCG TTGCTAAATC CGGTGTTATT GCAGGAATGG CAAATGAAGA TAAAAAAATC TTTACTGTTC AGTTCCACCC TGAAGTTTCT CACACAGAGG AAGGAAGCAA GATGCTGGAG AACTTTGTTC TAAACATCTG TCAGTCTGAA AGAAGTTGGA AGCTAACTAA TTATATTGAA AGAACAGTTG CCGAGATTAA AGAAAAAGTA GGCAGCCAGA AAGTAATTCT TGGACTCTCC GGAGGAGTAG ATTCATCTGT AGCAGCAGTA CTTATCCATA AAGCTATCGG AGATCAGTTG ACTTGTATTT TTGTAGATAC AGGGCTTCTG AGAAAAGATG AAGCAAAGAA AGTGATGGAG AACTACGGAC AGCATTTCCA CATGAACATA AAACTGGTAG ATGCTGCTGA CAGATTCTTG TCAAAGCTGG CTGGTGTTGA TGATCCTGAA CAAAAAAGAA AGATTATCGG AAACGAGTTT ATCGCTGTTT TCGATGAAGA ATCTCATAAA ATTGAAGGGG CAAAATTCTT AGCACAGGGA ACTATTTATC CTGATGTTAT CGAATCTCAG TCTGTAAAAG GACCTTCTGC AGTAATCAAG TCTCACCACA ATGTTGGTGG TCTTCCTGAG GAAATGGAAT TCGAATTGCT GGAACCGCTA AGAGAGCTAT TCAAAGACGA AGTAAGAAAA GTAGGAGAAG AACTTGGTAT TCCTCACCAC TTGGTATACA GACACCCATT CCCGGGTCCC GGATTAGGTA TCCGTGTTCT TGGAGCAGTA GATGTAGAAA AAGTAAGAAT CCTTCAGGAA GCTGATGATA TCTTCATCGA AGAATTGTAT AAAAATGATC TGTACGAAAA AGTATCTCAG GCTTTCGTAG TATTACTTCC GGTAAAATCT GTTGGAGTAA TGGGAGATGA GAGAACTTAT GAATATACAG CTGTTGTACG TTCTGCTAAT ACAATAGACT TTATGACGGC TACATGGAGT AAACTTCCTT ATGAATTCCT GGAAACTGTT TCCAACAGAA TTATCAATGA GGTAAGAGGT ATTAACCGTG TTGCTTACGA TATTTCAAGC AAACCACCTG CAACAATCGA GTGGGAA

Details for ElanA.17876.a.B1.GE40668

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	58kDa
OBSERVED MW:	59kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHME RGIIILDFGS QYNQLIGRRV REMGVYSEII PYYTPLEEIK ERNPFGIILS GGPSSVNAED AHLVSKEIFE LGIPVLGICY GMQLTAHLLG GKVAKGVKGE YGKAELEIVK ACSLFDGVKE RSIAWMSHFD EVETAPQGFD IVAKSGVIAG MANEDKKIFT VQFHPEVSHT EEGSKMLENF VLNICQSERS WKLTNYIERT VAEIKEKVGS QKVILGLSGG VDSSVAAVLI HKAIGDQLTC IFVDTGLLRK DEAKKVMENY GQHFHMNIKL VDAADRFLSK LAGVDDPEQK RKIIGNEFIA VFDEESHKIE GAKFLAQGTI YPDVIESQSV KGPSAVIKSH HNVGGLPEEM EFELLEPLRE LFKDEVRKVG EELGIPHHLV YRHPFPGPGL GIRVLGAVDV EKVRILQEAD DIFIEELYKN DLYEKVSQAF VVLLPVKSVG VMGDERTYEY TAVVRSANTI DFMTATWSKL PYEFLETVSN RIINEVRGIN RVAYDISSK

Validated NT Sequence

atggctcacc accaccacca ccatatggaa agaggaatta ttattttaga cttcggttca caatacaacc agttgatcgg aagaagagta agagagatgg gagtatattc tgaaattatc ccttactaca caccattgga agaaattaaa gaaagaaatc ctttcggtat tattctttca ggaggacctt cttctgtaaa tgcagaagat gcacatttgg tttcaaaaga aattttcgaa ttaggaattc ctgttttagg gatttgctat ggaatgcaat taacagctca ccttttggga ggtaaagttg ctaaaggtgt aaaaggagaa tatggaaaag ctgaattgga gatcgtaaaa gcatgttctt tattcgacgg agtaaaagaa agatctatag catggatgag ccacttcgac gaagtagaaa ctgctcctca gggattcgat atcgttgcta aatccggtgt tattgcagga atggcaaatg aagataaaaa aatctttact gttcagttcc accctgaagt ttctcacaca gaggaaggaa gcaagatgct ggagaacttt gttctaaaca tctgtcagtc tgaaagaagt tggaagctaa ctaattatat tgaaagaaca gttgccgaga ttaaagaaaa agtaggcagc cagaaagtaa ttcttggact ctccggagga gtagattcat ctgtagcagc agtacttatc cataaagcta tcggagatca gttgacttgt atttttgtag atacagggct tctgagaaaa gatgaagcaa agaaagtgat ggagaactac ggacagcatt tccacatgaa cataaaactg gtagatgctg ctgacagatt cttgtcaaag ctggctggtg ttgatgatcc tgaacaaaaa agaaagatta tcggaaacga gtttatcgct gttttcgatg aagaatctca taaaattgaa ggggcaaaat tcttagcaca gggaactatt tatcctgatg ttatcgaatc tcagtctgta aaaggacctt ctgcagtaat caagtctcac cacaatgttg gtggtcttcc tgaggaaatg gaattcgaat tgctggaacc gctaagagag ctattcaaag acgaagtaag aaaagtagga gaagaacttg gtattcctca ccacttggta tacagacacc cattcccggg tcccggatta ggtatccgtg ttcttggagc agtagatgta gaaaaagtaa gaatccttca ggaagctgat gatatcttca tcgaagaatt gtataaaaat gatctgtacg aaaaagtatc tcaggctttc gtagtattac ttccggtaaa atctgttgga gtaatgggag atgagagaac ttatgaatat acagctgttg tacgttctgc taatacaata gactttatga cggctacatg gagtaaactt ccttatgaat tcctggaaac tgtttccaac agaattatca atgaggtaag aggtattaac cgtgttgctt acgatatttc aagcaaacc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa gaggaattat tattttagac ttcggttcac aatacaacca gttgatcgga agaagagtaa gagagatggg agtatattct gaaattatcc cttactacac accattggaa gaaattaaag aaagaaatcc tttcggtatt attctttcag gaggaccttc ttctgtaaat gcagaagatg cacatttggt ttcaaaagaa attttcgaat taggaattcc tgttttaggg atttgctatg gaatgcaatt aacagctcac cttttgggag gtaaagttgc taaaggtgta aaaggagaat atggaaaagc tgaattggag atcgtaaaag catgttcttt attcgacgga gtaaaagaaa gatctatagc atggatgagc cacttcgacg aagtagaaac tgctcctcag ggattcgata tcgttgctaa atccggtgtt attgcaggaa tggcaaatga agataaaaaa atctttactg ttcagttcca ccctgaagtt tctcacacag aggaaggaag caagatgctg gagaactttg ttctaaacat ctgtcagtct gaaagaagtt ggaagctaac taattatatt gaaagaacag ttgccgagat taaagaaaaa gtaggcagcc agaaagtaat tcttggactc tccggaggag tagattcatc tgtagcagca gtacttatcc ataaagctat cggagatcag ttgacttgta tttttgtaga tacagggctt ctgagaaaag atgaagcaaa gaaagtgatg gagaactacg gacagcattt ccacatgaac ataaaactgg tagatgctgc tgacagattc ttgtcaaagc tggctggtgt tgatgatcct gaacaaaaaa gaaagattat cggaaacgag tttatcgctg ttttcgatga agaatctcat aaaattgaag gggcaaaatt cttagcacag ggaactattt atcctgatgt tatcgaatct cagtctgtaa aaggaccttc tgcagtaatc aagtctcacc acaatgttgg tggtcttcct gaggaaatgg aattcgaatt gctggaaccg ctaagagagc tattcaaaga cgaagtaaga aaagtaggag aagaacttgg tattcctcac cacttggtat acagacaccc attcccgggt cccggattag gtatccgtgt tcttggagca gtagatgtag aaaaagtaag aatccttcag gaagctgatg atatcttcat cgaagaattg tataaaaatg atctgtacga aaaagtatct caggctttcg tagtattact tccggtaaaa tctgttggag taatgggaga tgagagaact tatgaatata cagctgttgt acgttctgct aatacaatag actttatgac ggctacatgg agtaaacttc cttatgaatt cctggaaact gtttccaaca gaattatcaa tgaggtaaga ggtattaacc gtgttgctta cgatatttca agcaaaccac ctgcaacaat cgagtgggaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for ElanA.17876.a.B1.PW38261

PURIFICATION DATe:	5/1/2017
CONCENTRATION:	20mg/ml
OBSERVED MW:	55kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAA GAGGAATTAT TATTTTAGAC TTCGGTTCAC AATACAACCA GTTGATCGGA AGAAGAGTAA GAGAGATGGG AGTATATTCT GAAATTATCC CTTACTACAC ACCATTGGAA GAAATTAAAG AAAGAAATCC TTTCGGTATT ATTCTTTCAG GAGGACCTTC TTCTGTAAAT GCAGAAGATG CACATTTGGT TTCAAAAGAA ATTTTCGAAT TAGGAATTCC TGTTTTAGGG ATTTGCTATG GAATGCAATT AACAGCTCAC CTTTTGGGAG GTAAAGTTGC TAAAGGTGTA AAAGGAGAAT ATGGAAAAGC TGAATTGGAG ATCGTAAAAG CATGTTCTTT ATTCGACGGA GTAAAAGAAA GATCTATAGC ATGGATGAGC CACTTCGACG AAGTAGAAAC TGCTCCTCAG GGATTCGATA TCGTTGCTAA ATCCGGTGTT ATTGCAGGAA TGGCAAATGA AGATAAAAAA ATCTTTACTG TTCAGTTCCA CCCTGAAGTT TCTCACACAG AGGAAGGAAG CAAGATGCTG GAGAACTTTG TTCTAAACAT CTGTCAGTCT GAAAGAAGTT GGAAGCTAAC TAATTATATT GAAAGAACAG TTGCCGAGAT TAAAGAAAAA GTAGGCAGCC AGAAAGTAAT TCTTGGACTC TCCGGAGGAG TAGATTCATC TGTAGCAGCA GTACTTATCC ATAAAGCTAT CGGAGATCAG TTGACTTGTA TTTTTGTAGA TACAGGGCTT CTGAGAAAAG ATGAAGCAAA GAAAGTGATG GAGAACTACG GACAGCATTT CCACATGAAC ATAAAACTGG TAGATGCTGC TGACAGATTC TTGTCAAAGC TGGCTGGTGT TGATGATCCT GAACAAAAAA GAAAGATTAT CGGAAACGAG TTTATCGCTG TTTTCGATGA AGAATCTCAT AAAATTGAAG GGGCAAAATT CTTAGCACAG GGAACTATTT ATCCTGATGT TATCGAATCT CAGTCTGTAA AAGGACCTTC TGCAGTAATC AAGTCTCACC ACAATGTTGG TGGTCTTCCT GAGGAAATGG AATTCGAATT GCTGGAACCG CTAAGAGAGC TATTCAAAGA CGAAGTAAGA AAAGTAGGAG AAGAACTTGG TATTCCTCAC CACTTGGTAT ACAGACACCC ATTCCCGGGT CCCGGATTAG GTATCCGTGT TCTTGGAGCA GTAGATGTAG AAAAAGTAAG AATCCTTCAG GAAGCTGATG ATATCTTCAT CGAAGAATTG TATAAAAATG ATCTGTACGA AAAAGTATCT CAGGCTTTCG TAGTATTACT TCCGGTAAAA TCTGTTGGAG TAATGGGAGA TGAGAGAACT TATGAATATA CAGCTGTTGT ACGTTCTGCT AATACAATAG ACTTTATGAC GGCTACATGG AGTAAACTTC CTTATGAATT CCTGGAAACT GTTTCCAACA GAATTATCAA TGAGGTAAGA GGTATTAACC GTGTTGCTTA CGATATTTCA AGCAAACCAC CTGCAACAAT CGAGTGGGAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT