ElanA.17803.a

Macro domain, possibly ADP-ribose binding module

CENTER ID: ElanA.17803.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.17803.a.B1.GE40667 Full length(ElanA.17803.a) 1 172
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.171
UniProt: A0A077EQP9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTTIEIIKG DITKIQAYVI VNAANSSLLG GGGVDGAIHR AGGKQILDEC IRIRNKQGKC NPGEAVVTTA GNLPAQYVIH TVGPVWKGDK ERCERLLGNC YNNALELAGN LGVKTIAFPN ISTGIYKFPK DLAAEIAVKI AQNLESETIE KIIFVCFDEE NERIYKQRLN DN
NT Sequence
ATGAAAACAA CGATTGAAAT TATAAAAGGA GATATCACAA AAATTCAGGC ATATGTGATA GTTAATGCGG CTAATTCTTC ATTATTAGGA GGTGGTGGAG TTGATGGAGC GATCCATCGT GCCGGGGGAA AACAAATCCT TGATGAATGT ATTCGGATCA GAAATAAACA AGGAAAATGT AATCCCGGTG AAGCCGTTGT TACAACGGCA GGTAACCTTC CCGCACAGTA TGTCATTCAT ACCGTTGGAC CTGTATGGAA AGGTGATAAA GAAAGATGTG AAAGATTGTT GGGCAATTGC TATAATAACG CATTAGAACT GGCAGGTAAT CTGGGGGTTA AAACAATTGC TTTCCCTAAT ATAAGTACAG GAATTTATAA GTTTCCAAAA GATCTGGCAG CGGAAATAGC AGTTAAAATA GCTCAAAATT TAGAGTCCGA GACAATAGAA AAAATTATTT TTGTTTGCTT TGATGAAGAA AATGAGAGGA TATACAAGCA ACGTTTAAAT GATAAT
Details for ElanA.17803.a.B1.GE40667
HARVESTED ON: data unavailable
SEQUENCED ON: 11/8/2016
EXPECTED MW: 20kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK TTIEIIKGDI TKIQAYVIVN AANSSLLGGG GVDGAIHRAG GKQILDECIR IRNKQGKCNP GEAVVTTAGN LPAQYVIHTV GPVWKGDKER CERLLGNCYN NALELAGNLG VKTIAFPNIS TGIYKFPKDL AAEIAVKIAQ NLESETIEKI IFVCFDEENE RIYKQRLNDN
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa acaacgattg aaattataaa aggagatatc acaaaaattc aggcatatgt gatagttaat gcggctaatt cttcattatt aggaggtggt ggagttgatg gagcgatcca tcgtgccggg ggaaaacaaa tccttgatga atgtattcgg atcagaaata aacaaggaaa atgtaatccc ggtgaagccg ttgttacaac ggcaggtaac cttcccgcac agtatgtcat tcataccgtt ggacctgtat ggaaaggtga taaagaaaga tgtgaaagat tgttgggcaa ttgctataat aacgcattag aactggcagg taatctgggg gttaaaacaa ttgctttccc taatataagt acaggaattt ataagtttcc aaaagatctg gcagcggaaa tagcagttaa aatagctcaa aatttagagt ccgagacaat agaaaaaatt atttttgttt gctttgatga agaaaatgag aggatataca agcaacgttt aaatgataat taagtgagta agat
Expected Protein Sequence
MAHHHHHHMK TTIEIIKGDI TKIQAYVIVN AANSSLLGGG GVDGAIHRAG GKQILDECIR IRNKQGKCNP GEAVVTTAGN LPAQYVIHTV GPVWKGDKER CERLLGNCYN NALELAGNLG VKTIAFPNIS TGIYKFPKDL AAEIAVKIAQ NLESETIEKI IFVCFDEENE RIYKQRLNDN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa caacgattga aattataaaa ggagatatca caaaaattca ggcatatgtg atagttaatg cggctaattc ttcattatta ggaggtggtg gagttgatgg agcgatccat cgtgccgggg gaaaacaaat ccttgatgaa tgtattcgga tcagaaataa acaaggaaaa tgtaatcccg gtgaagccgt tgttacaacg gcaggtaacc ttcccgcaca gtatgtcatt cataccgttg gacctgtatg gaaaggtgat aaagaaagat gtgaaagatt gttgggcaat tgctataata acgcattaga actggcaggt aatctggggg ttaaaacaat tgctttccct aatataagta caggaattta taagtttcca aaagatctgg cagcggaaat agcagttaaa atagctcaaa atttagagtc cgagacaata gaaaaaatta tttttgtttg ctttgatgaa gaaaatgaga ggatatacaa gcaacgttta aatgataatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat