ElanA.17354.a

Tryptophan synthase beta chain

CENTER ID: ElanA.17354.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.17354.a.B1.GE40666 Full length(ElanA.17354.a) 1 393
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.17354.a.B1.PW38375 Full length(ElanA.17354.a) 1 393

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.826
UniProt: A0A077EEA2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKYKNPDQYG YYGDFGGAFI PEMLYPNVKE LEDNYLDIIN SDDFRKEYNA LLKDYVGRAT PLYFAKNLSN KYQTNIYLKR EDLNHTGAHK INNALGQALL AKRLSKNRII AETGAGQHGV ATATACALLG LECIVYMGEI DIARQAPNVA RMKMLGATVI PATSGSKTLK DAVNEALRDW INNASTTHYI IGSVVGPHPF PDMVARFQSI ISEEIRFQLK EHIGRENPDY VIACVGGGSN AAGTFYHFVD EPGVGIIAAE AGGLGKDSGE SAATTFLGTL GILHGSQSLV MQTEDGQVIE PYSISAGLDY PGIGPFHAHL FQEKRAEFFS INDDEALKSA FELTRLEGII PALESAHALA VLDQKKFDKE NVVVICLSGR GDKDMETYLK HLA
NT Sequence
ATGAAATACA AAAATCCTGA TCAATACGGA TATTATGGAG ATTTCGGCGG AGCATTTATC CCTGAAATGC TTTATCCAAA TGTAAAAGAA TTAGAAGACA ATTATCTGGA TATTATCAAT AGTGATGATT TCAGAAAAGA ATATAATGCT CTGCTTAAAG ATTATGTAGG GAGGGCTACT CCACTTTATT TTGCTAAGAA TCTCAGCAAT AAATACCAGA CAAATATATA TCTTAAAAGA GAAGATCTAA ACCATACAGG TGCTCATAAG ATTAATAACG CGCTTGGGCA AGCTCTGCTG GCAAAGCGCC TGAGCAAAAA CAGAATTATT GCAGAAACCG GAGCTGGACA GCATGGTGTG GCTACTGCAA CAGCTTGTGC CCTATTGGGT CTGGAATGTA TTGTTTATAT GGGTGAAATA GACATTGCCC GTCAGGCTCC AAATGTTGCC CGAATGAAAA TGCTTGGCGC AACTGTAATC CCTGCAACGT CCGGAAGCAA AACCCTAAAA GATGCTGTAA ACGAGGCTTT AAGAGACTGG ATTAACAATG CTTCTACAAC CCATTACATT ATTGGAAGTG TTGTAGGCCC TCACCCTTTT CCGGATATGG TGGCAAGATT CCAGAGTATT ATAAGTGAAG AAATAAGATT CCAACTGAAA GAACATATCG GGCGCGAGAA TCCTGATTAC GTTATTGCTT GTGTAGGTGG TGGAAGTAAT GCTGCAGGGA CCTTTTATCA TTTTGTAGAT GAACCTGGTG TTGGCATTAT TGCTGCAGAA GCTGGCGGTC TGGGTAAAGA TTCCGGTGAG AGCGCCGCAA CCACCTTTTT GGGAACATTA GGAATTTTAC ACGGCAGCCA AAGTCTTGTA ATGCAGACAG AAGACGGACA GGTTATAGAA CCTTACAGTA TCTCTGCAGG GCTGGATTAT CCAGGTATTG GTCCATTTCA TGCGCATTTA TTCCAGGAGA AAAGAGCAGA GTTTTTCAGT ATAAATGATG ATGAGGCATT GAAATCCGCT TTTGAACTTA CCAGACTTGA AGGAATTATT CCAGCACTGG AATCCGCACA TGCTTTAGCT GTATTGGATC AGAAAAAATT CGATAAAGAG AATGTTGTTG TAATATGCCT TAGCGGGCGT GGTGATAAGG ATATGGAAAC TTATTTGAAG CATTTAGCT
Details for ElanA.17354.a.B1.GE40666
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 44kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMK YKNPDQYGYY GDFGGAFIPE MLYPNVKELE DNYLDIINSD DFRKEYNALL KDYVGRATPL YFAKNLSNKY QTNIYLKRED LNHTGAHKIN NALGQALLAK RLSKNRIIAE TGAGQHGVAT ATACALLGLE CIVYMGEIDI ARQAPNVARM KMLGATVIPA TSGSKTLKDA VNEALRDWIN NASTTHYIIG SVVGPHPFPD MVARFQSIIS EEIRFQLKEH IGRENPDYVI ACVGGGSNAA GTFYHFVDEP GVGIIAAEAG GLGKDSGESA ATTFLGTLGI LHGSQSLVMQ TEDGQVIEPY SISAGLDYPG IGPFHAHLFQ EKRAEFFSIN DDEALKSAFE LTRLEGIIPA LESAHALAVL DQKKFDKENV VVICLSGRGD KDM
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa tacaaaaatc ctgatcaata cggatattat ggagatttcg gcggagcatt tatccctgaa atgctttatc caaatgtaaa agaattagaa gacaattatc tggatattat caatagtgat gatttcagaa aagaatataa tgctctgctt aaagattatg tagggagggc tactccactt tattttgcta agaatctcag caataaatac cagacaaata tatatcttaa aagagaagat ctaaaccata caggtgctca taagattaat aacgcgcttg ggcaagctct gctggcaaag cgcctgagca aaaacagaat tattgcagaa accggagctg gacagcatgg tgtggctact gcaacagctt gtgccctatt gggtctggaa tgtattgttt atatgggtga aatagacatt gcccgtcagg ctccaaatgt tgcccgaatg aaaatgcttg gcgcaactgt aatccctgca acgtccggaa gcaaaaccct aaaagatgct gtaaacgagg ctttaagaga ctggattaac aatgcttcta caacccatta cattattgga agtgttgtag gccctcaccc ttttccggat atggtggcaa gattccagag tattataagt gaagaaataa gattccaact gaaagaacat atcgggcgcg agaatcctga ttacgttatt gcttgtgtag gtggtggaag taatgctgca gggacctttt atcattttgt agatgaacct ggtgttggca ttattgctgc agaagctggc ggtctgggta aagattccgg tgagagcgcc gcaaccacct ttttgggaac attaggaatt ttacacggca gccaaagtct tgtaatgcag acagaagacg gacaggttat agaaccttac agtatctctg cagggctgga ttatccaggt attggtccat ttcatgcgca tttattccag gagaaaagag cagagttttt cagtataaat gatgatgagg cattgaaatc cgcttttgaa cttaccagac ttgaaggaat tattccagca ctggaatccg cacatgcttt agctgtattg gatcagaaaa aattcgataa agagaatgtt gttgtaatat gccttagcgg gcgtggtgat aaggatatgg aa
Expected Protein Sequence
MAHHHHHHMK YKNPDQYGYY GDFGGAFIPE MLYPNVKELE DNYLDIINSD DFRKEYNALL KDYVGRATPL YFAKNLSNKY QTNIYLKRED LNHTGAHKIN NALGQALLAK RLSKNRIIAE TGAGQHGVAT ATACALLGLE CIVYMGEIDI ARQAPNVARM KMLGATVIPA TSGSKTLKDA VNEALRDWIN NASTTHYIIG SVVGPHPFPD MVARFQSIIS EEIRFQLKEH IGRENPDYVI ACVGGGSNAA GTFYHFVDEP GVGIIAAEAG GLGKDSGESA ATTFLGTLGI LHGSQSLVMQ TEDGQVIEPY SISAGLDYPG IGPFHAHLFQ EKRAEFFSIN DDEALKSAFE LTRLEGIIPA LESAHALAVL DQKKFDKENV VVICLSGRGD KDMETYLKHL A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat acaaaaatcc tgatcaatac ggatattatg gagatttcgg cggagcattt atccctgaaa tgctttatcc aaatgtaaaa gaattagaag acaattatct ggatattatc aatagtgatg atttcagaaa agaatataat gctctgctta aagattatgt agggagggct actccacttt attttgctaa gaatctcagc aataaatacc agacaaatat atatcttaaa agagaagatc taaaccatac aggtgctcat aagattaata acgcgcttgg gcaagctctg ctggcaaagc gcctgagcaa aaacagaatt attgcagaaa ccggagctgg acagcatggt gtggctactg caacagcttg tgccctattg ggtctggaat gtattgttta tatgggtgaa atagacattg cccgtcaggc tccaaatgtt gcccgaatga aaatgcttgg cgcaactgta atccctgcaa cgtccggaag caaaacccta aaagatgctg taaacgaggc tttaagagac tggattaaca atgcttctac aacccattac attattggaa gtgttgtagg ccctcaccct tttccggata tggtggcaag attccagagt attataagtg aagaaataag attccaactg aaagaacata tcgggcgcga gaatcctgat tacgttattg cttgtgtagg tggtggaagt aatgctgcag ggacctttta tcattttgta gatgaacctg gtgttggcat tattgctgca gaagctggcg gtctgggtaa agattccggt gagagcgccg caaccacctt tttgggaaca ttaggaattt tacacggcag ccaaagtctt gtaatgcaga cagaagacgg acaggttata gaaccttaca gtatctctgc agggctggat tatccaggta ttggtccatt tcatgcgcat ttattccagg agaaaagagc agagtttttc agtataaatg atgatgaggc attgaaatcc gcttttgaac ttaccagact tgaaggaatt attccagcac tggaatccgc acatgcttta gctgtattgg atcagaaaaa attcgataaa gagaatgttg ttgtaatatg ccttagcggg cgtggtgata aggatatgga aacttatttg aagcatttag cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for ElanA.17354.a.B1.PW38375
PURIFICATION DATe: 10/17/2017
CONCENTRATION: 27.88mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
IMAC->SEC Started to crash out during concentration
Validated AA Sequence
MAHHHHHHMK YKNPDQYGYY GDFGGAFIPE MLYPNVKELE DNYLDIINSD DFRKEYNALL KDYVGRATPL YFAKNLSNKY QTNIYLKRED LNHTGAHKIN NALGQALLAK RLSKNRIIAE TGAGQHGVAT ATACALLGLE CIVYMGEIDI ARQAPNVARM KMLGATVIPA TSGSKTLKDA VNEALRDWIN NASTTHYIIG SVVGPHPFPD MVARFQSIIS EEIRFQLKEH IGRENPDYVI ACVGGGSNAA GTFYHFVDEP GVGIIAAEAG GLGKDSGESA ATTFLGTLGI LHGSQSLVMQ TEDGQVIEPY SISAGLDYPG IGPFHAHLFQ EKRAEFFSIN DDEALKSAFE LTRLEGIIPA LESAHALAVL DQKKFDKENV VVICLSGRGD KDM
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa tacaaaaatc ctgatcaata cggatattat ggagatttcg gcggagcatt tatccctgaa atgctttatc caaatgtaaa agaattagaa gacaattatc tggatattat caatagtgat gatttcagaa aagaatataa tgctctgctt aaagattatg tagggagggc tactccactt tattttgcta agaatctcag caataaatac cagacaaata tatatcttaa aagagaagat ctaaaccata caggtgctca taagattaat aacgcgcttg ggcaagctct gctggcaaag cgcctgagca aaaacagaat tattgcagaa accggagctg gacagcatgg tgtggctact gcaacagctt gtgccctatt gggtctggaa tgtattgttt atatgggtga aatagacatt gcccgtcagg ctccaaatgt tgcccgaatg aaaatgcttg gcgcaactgt aatccctgca acgtccggaa gcaaaaccct aaaagatgct gtaaacgagg ctttaagaga ctggattaac aatgcttcta caacccatta cattattgga agtgttgtag gccctcaccc ttttccggat atggtggcaa gattccagag tattataagt gaagaaataa gattccaact gaaagaacat atcgggcgcg agaatcctga ttacgttatt gcttgtgtag gtggtggaag taatgctgca gggacctttt atcattttgt agatgaacct ggtgttggca ttattgctgc agaagctggc ggtctgggta aagattccgg tgagagcgcc gcaaccacct ttttgggaac attaggaatt ttacacggca gccaaagtct tgtaatgcag acagaagacg gacaggttat agaaccttac agtatctctg cagggctgga ttatccaggt attggtccat ttcatgcgca tttattccag gagaaaagag cagagttttt cagtataaat gatgatgagg cattgaaatc cgcttttgaa cttaccagac ttgaaggaat tattccagca ctggaatccg cacatgcttt agctgtattg gatcagaaaa aattcgataa agagaatgtt gttgtaatat gccttagcgg gcgtggtgat aaggatatgg aa
Expressed Protein Sequence
MAHHHHHHMK YKNPDQYGYY GDFGGAFIPE MLYPNVKELE DNYLDIINSD DFRKEYNALL KDYVGRATPL YFAKNLSNKY QTNIYLKRED LNHTGAHKIN NALGQALLAK RLSKNRIIAE TGAGQHGVAT ATACALLGLE CIVYMGEIDI ARQAPNVARM KMLGATVIPA TSGSKTLKDA VNEALRDWIN NASTTHYIIG SVVGPHPFPD MVARFQSIIS EEIRFQLKEH IGRENPDYVI ACVGGGSNAA GTFYHFVDEP GVGIIAAEAG GLGKDSGESA ATTFLGTLGI LHGSQSLVMQ TEDGQVIEPY SISAGLDYPG IGPFHAHLFQ EKRAEFFSIN DDEALKSAFE LTRLEGIIPA LESAHALAVL DQKKFDKENV VVICLSGRGD KDMETYLKHL A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAT ACAAAAATCC TGATCAATAC GGATATTATG GAGATTTCGG CGGAGCATTT ATCCCTGAAA TGCTTTATCC AAATGTAAAA GAATTAGAAG ACAATTATCT GGATATTATC AATAGTGATG ATTTCAGAAA AGAATATAAT GCTCTGCTTA AAGATTATGT AGGGAGGGCT ACTCCACTTT ATTTTGCTAA GAATCTCAGC AATAAATACC AGACAAATAT ATATCTTAAA AGAGAAGATC TAAACCATAC AGGTGCTCAT AAGATTAATA ACGCGCTTGG GCAAGCTCTG CTGGCAAAGC GCCTGAGCAA AAACAGAATT ATTGCAGAAA CCGGAGCTGG ACAGCATGGT GTGGCTACTG CAACAGCTTG TGCCCTATTG GGTCTGGAAT GTATTGTTTA TATGGGTGAA ATAGACATTG CCCGTCAGGC TCCAAATGTT GCCCGAATGA AAATGCTTGG CGCAACTGTA ATCCCTGCAA CGTCCGGAAG CAAAACCCTA AAAGATGCTG TAAACGAGGC TTTAAGAGAC TGGATTAACA ATGCTTCTAC AACCCATTAC ATTATTGGAA GTGTTGTAGG CCCTCACCCT TTTCCGGATA TGGTGGCAAG ATTCCAGAGT ATTATAAGTG AAGAAATAAG ATTCCAACTG AAAGAACATA TCGGGCGCGA GAATCCTGAT TACGTTATTG CTTGTGTAGG TGGTGGAAGT AATGCTGCAG GGACCTTTTA TCATTTTGTA GATGAACCTG GTGTTGGCAT TATTGCTGCA GAAGCTGGCG GTCTGGGTAA AGATTCCGGT GAGAGCGCCG CAACCACCTT TTTGGGAACA TTAGGAATTT TACACGGCAG CCAAAGTCTT GTAATGCAGA CAGAAGACGG ACAGGTTATA GAACCTTACA GTATCTCTGC AGGGCTGGAT TATCCAGGTA TTGGTCCATT TCATGCGCAT TTATTCCAGG AGAAAAGAGC AGAGTTTTTC AGTATAAATG ATGATGAGGC ATTGAAATCC GCTTTTGAAC TTACCAGACT TGAAGGAATT ATTCCAGCAC TGGAATCCGC ACATGCTTTA GCTGTATTGG ATCAGAAAAA ATTCGATAAA GAGAATGTTG TTGTAATATG CCTTAGCGGG CGTGGTGATA AGGATATGGA AACTTATTTG AAGCATTTAG CTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT