ElanA.10611.a
Alcohol dehydrogenase
| CENTER ID: | ElanA.10611.a |
| ORGANISM: | Elizabethkingia anophelis NUHP1 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ElanA.10611.a.B1.GE40664 | Full length(ElanA.10611.a) | 1 | 345 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ElanA.10611.a.B1.PS38362 | Full length(ElanA.10611.a) | 1 | 345 |
Structures
6N7L
DEPOSITED: 11/27/2018
DETERMINATION: XRay
CLONE: ElanA.10611.a.B1.GE40664
PROTEIN: ElanA.10611.a.B1.PS38362
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1338011.4.peg.3682 |
| UniProt: | A0A077EGR8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIPKTMKAAV VQGYGEPLKI QEVPVREPGR YEVLVKVMAC GVCHTDLHAV DGDWPAKPKM PLIPGHEGVG IVVACGPDAM VKEGDAVGVP WLYSACGCCD YCITGWETLC EAQQNGGYSV DGGFAEYVIA DSRYVGHLKS NVNFLEIAPI LCAGVTVYKG LKETETKPGE WVAISGIGGL GHVAVQYAKA MGMHVAAIDV ADDKLELAKK LGADLTVNAK TTDPGTYLHK EVGGMHGALI TAVSPIAFKQ GIDVLRRKGT IALNGLPPGS FELPIFETVL KRITVRGSIV GTRKDLQEAL DFANEGLVKA TVTSAKLEDI NDVFDKMKKG QIDGRIVLDI AGSQN
NT Sequence
ATGATTCCAA AAACAATGAA AGCAGCGGTT GTCCAGGGTT ATGGAGAACC ATTAAAGATT CAGGAAGTAC CGGTAAGAGA ACCTGGCCGT TATGAAGTAT TGGTAAAAGT AATGGCGTGC GGAGTCTGTC ATACCGATCT ACATGCTGTA GACGGAGACT GGCCGGCTAA ACCCAAAATG CCGTTGATTC CGGGACATGA AGGTGTTGGT ATCGTCGTTG CTTGTGGTCC GGATGCTATG GTAAAAGAAG GTGATGCTGT CGGAGTTCCT TGGTTATATA GTGCTTGTGG ATGCTGTGAT TATTGTATAA CAGGCTGGGA AACGCTTTGT GAAGCCCAAC AGAATGGAGG ATATAGTGTA GATGGAGGAT TTGCTGAATA TGTAATTGCA GATTCCCGAT ATGTGGGACA TCTGAAATCT AATGTCAATT TTCTAGAGAT TGCGCCAATT TTGTGTGCAG GTGTTACTGT CTATAAAGGA TTAAAAGAAA CAGAAACCAA ACCTGGTGAA TGGGTTGCGA TTTCCGGAAT TGGCGGATTA GGACATGTTG CCGTACAGTA TGCAAAGGCA ATGGGAATGC ATGTCGCAGC TATAGATGTT GCAGATGATA AACTGGAGCT TGCAAAAAAG CTAGGAGCAG ATCTTACGGT AAATGCAAAA ACAACCGATC CGGGTACCTA TTTGCATAAA GAAGTAGGTG GAATGCATGG GGCTTTGATA ACAGCTGTTT CTCCGATTGC CTTTAAACAA GGAATCGATG TGCTCAGAAG AAAAGGAACT ATTGCGCTGA ATGGGCTTCC TCCGGGATCA TTTGAATTGC CAATTTTTGA AACGGTACTA AAGAGGATTA CAGTAAGAGG CTCTATTGTA GGGACAAGAA AAGATTTGCA GGAGGCATTG GACTTTGCCA ATGAAGGCTT GGTAAAAGCT ACAGTAACTT CAGCAAAATT GGAAGATATT AATGATGTTT TTGATAAAAT GAAAAAAGGC CAGATCGACG GAAGAATAGT TCTGGATATT GCAGGAAGTC AAAAT
Details for ElanA.10611.a.B1.GE40664
| HARVESTED ON: | 11/8/2016 |
| SEQUENCED ON: | 11/8/2016 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMI PKTMKAAVVQ GYGEPLKIQE VPVREPGRYE VLVKVMACGV CHTDLHAVDG DWPAKPKMPL IPGHEGVGIV VACGPDAMVK EGDAVGVPWL YSACGCCDYC ITGWETLCEA QQNGGYSVDG GFAEYVIADS RYVGHLKSNV NFLEIAPILC AGVTVYKGLK ETETKPGEWV AISGIGGLGH VAVQYAKAMG MHVAAIDVAD DKLELAKKLG ADLTVNAKTT DPGTYLHKEV GGMHGALITA VSPIAFKQGI DVLRRKGTIA LNGLPPGSFE LPIFETVLKR ITVRGSIVGT RKDLQEALDF ANEGLVKATV TSAKLEDIND VFDKMKKGQI DGRIV
Validated NT Sequence
atataccatg gctcaccacc accaccacca tatgattcca aaaacaatga aagcagcggt tgtccagggt tatggagaac cattaaagat tcaggaagta ccggtaagag aacctggccg ttatgaagta ttggtaaaag taatggcgtg cggagtctgt cataccgatc tacatgctgt agacggagac tggccggcta aacccaaaat gccgttgatt ccgggacatg aaggtgttgg tatcgtcgtt gcttgtggtc cggatgctat ggtaaaagaa ggtgatgctg tcggagttcc ttggttatat agtgcttgtg gatgctgtga ttattgtata acaggctggg aaacgctttg tgaagcccaa cagaatggag gatatagtgt agatggagga tttgctgaat atgtaattgc agattcccga tatgtgggac atctgaaatc taatgtcaat tttctagaga ttgcgccaat tttgtgtgca ggtgttactg tctataaagg attaaaagaa acagaaacca aacctggtga atgggttgcg atttccggaa ttggcggatt aggacatgtt gccgtacagt atgcaaaggc aatgggaatg catgtcgcag ctatagatgt tgcagatgat aaactggagc ttgcaaaaaa gctaggagca gatcttacgg taaatgcaaa aacaaccgat ccgggtacct atttgcataa agaagtaggt ggaatgcatg gggctttgat aacagctgtt tctccgattg cctttaaaca aggaatcgat gtgctcagaa gaaaaggaac tattgcgctg aatgggcttc ctccgggatc atttgaattg ccaatttttg aaacggtact aaagaggatt acagtaagag gctctattgt agggacaaga aaagatttgc aggaggcatt ggactttgcc aatgaaggct tggtaaaagc tacagtaact tcagcaaaat tggaagatat taatgatgtt tttgataaaa tgaaaaaagg ccagatcgac ggaagaatag ttc
Expected Protein Sequence
MAHHHHHHMI PKTMKAAVVQ GYGEPLKIQE VPVREPGRYE VLVKVMACGV CHTDLHAVDG DWPAKPKMPL IPGHEGVGIV VACGPDAMVK EGDAVGVPWL YSACGCCDYC ITGWETLCEA QQNGGYSVDG GFAEYVIADS RYVGHLKSNV NFLEIAPILC AGVTVYKGLK ETETKPGEWV AISGIGGLGH VAVQYAKAMG MHVAAIDVAD DKLELAKKLG ADLTVNAKTT DPGTYLHKEV GGMHGALITA VSPIAFKQGI DVLRRKGTIA LNGLPPGSFE LPIFETVLKR ITVRGSIVGT RKDLQEALDF ANEGLVKATV TSAKLEDIND VFDKMKKGQI DGRIVLDIAG SQN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattc caaaaacaat gaaagcagcg gttgtccagg gttatggaga accattaaag attcaggaag taccggtaag agaacctggc cgttatgaag tattggtaaa agtaatggcg tgcggagtct gtcataccga tctacatgct gtagacggag actggccggc taaacccaaa atgccgttga ttccgggaca tgaaggtgtt ggtatcgtcg ttgcttgtgg tccggatgct atggtaaaag aaggtgatgc tgtcggagtt ccttggttat atagtgcttg tggatgctgt gattattgta taacaggctg ggaaacgctt tgtgaagccc aacagaatgg aggatatagt gtagatggag gatttgctga atatgtaatt gcagattccc gatatgtggg acatctgaaa tctaatgtca attttctaga gattgcgcca attttgtgtg caggtgttac tgtctataaa ggattaaaag aaacagaaac caaacctggt gaatgggttg cgatttccgg aattggcgga ttaggacatg ttgccgtaca gtatgcaaag gcaatgggaa tgcatgtcgc agctatagat gttgcagatg ataaactgga gcttgcaaaa aagctaggag cagatcttac ggtaaatgca aaaacaaccg atccgggtac ctatttgcat aaagaagtag gtggaatgca tggggctttg ataacagctg tttctccgat tgcctttaaa caaggaatcg atgtgctcag aagaaaagga actattgcgc tgaatgggct tcctccggga tcatttgaat tgccaatttt tgaaacggta ctaaagagga ttacagtaag aggctctatt gtagggacaa gaaaagattt gcaggaggca ttggactttg ccaatgaagg cttggtaaaa gctacagtaa cttcagcaaa attggaagat attaatgatg tttttgataa aatgaaaaaa ggccagatcg acggaagaat agttctggat attgcaggaa gtcaaaattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for ElanA.10611.a.B1.PS38362
| PURIFICATION DATe: | 10/11/2017 |
| CONCENTRATION: | 54.26mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI PKTMKAAVVQ GYGEPLKIQE VPVREPGRYE VLVKVMACGV CHTDLHAVDG DWPAKPKMPL IPGHEGVGIV VACGPDAMVK EGDAVGVPWL YSACGCCDYC ITGWETLCEA QQNGGYSVDG GFAEYVIADS RYVGHLKSNV NFLEIAPILC AGVTVYKGLK ETETKPGEWV AISGIGGLGH VAVQYAKAMG MHVAAIDVAD DKLELAKKLG ADLTVNAKTT DPGTYLHKEV GGMHGALITA VSPIAFKQGI DVLRRKGTIA LNGLPPGSFE LPIFETVLKR ITVRGSIVGT RKDLQEALDF ANEGLVKATV TSAKLEDIND VFDKMKKGQI DGRIV
Validated NT Sequence
atataccatg gctcaccacc accaccacca tatgattcca aaaacaatga aagcagcggt tgtccagggt tatggagaac cattaaagat tcaggaagta ccggtaagag aacctggccg ttatgaagta ttggtaaaag taatggcgtg cggagtctgt cataccgatc tacatgctgt agacggagac tggccggcta aacccaaaat gccgttgatt ccgggacatg aaggtgttgg tatcgtcgtt gcttgtggtc cggatgctat ggtaaaagaa ggtgatgctg tcggagttcc ttggttatat agtgcttgtg gatgctgtga ttattgtata acaggctggg aaacgctttg tgaagcccaa cagaatggag gatatagtgt agatggagga tttgctgaat atgtaattgc agattcccga tatgtgggac atctgaaatc taatgtcaat tttctagaga ttgcgccaat tttgtgtgca ggtgttactg tctataaagg attaaaagaa acagaaacca aacctggtga atgggttgcg atttccggaa ttggcggatt aggacatgtt gccgtacagt atgcaaaggc aatgggaatg catgtcgcag ctatagatgt tgcagatgat aaactggagc ttgcaaaaaa gctaggagca gatcttacgg taaatgcaaa aacaaccgat ccgggtacct atttgcataa agaagtaggt ggaatgcatg gggctttgat aacagctgtt tctccgattg cctttaaaca aggaatcgat gtgctcagaa gaaaaggaac tattgcgctg aatgggcttc ctccgggatc atttgaattg ccaatttttg aaacggtact aaagaggatt acagtaagag gctctattgt agggacaaga aaagatttgc aggaggcatt ggactttgcc aatgaaggct tggtaaaagc tacagtaact tcagcaaaat tggaagatat taatgatgtt tttgataaaa tgaaaaaagg ccagatcgac ggaagaatag ttc
Expressed Protein Sequence
MAHHHHHHMI PKTMKAAVVQ GYGEPLKIQE VPVREPGRYE VLVKVMACGV CHTDLHAVDG DWPAKPKMPL IPGHEGVGIV VACGPDAMVK EGDAVGVPWL YSACGCCDYC ITGWETLCEA QQNGGYSVDG GFAEYVIADS RYVGHLKSNV NFLEIAPILC AGVTVYKGLK ETETKPGEWV AISGIGGLGH VAVQYAKAMG MHVAAIDVAD DKLELAKKLG ADLTVNAKTT DPGTYLHKEV GGMHGALITA VSPIAFKQGI DVLRRKGTIA LNGLPPGSFE LPIFETVLKR ITVRGSIVGT RKDLQEALDF ANEGLVKATV TSAKLEDIND VFDKMKKGQI DGRIVLDIAG SQN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATTC CAAAAACAAT GAAAGCAGCG GTTGTCCAGG GTTATGGAGA ACCATTAAAG ATTCAGGAAG TACCGGTAAG AGAACCTGGC CGTTATGAAG TATTGGTAAA AGTAATGGCG TGCGGAGTCT GTCATACCGA TCTACATGCT GTAGACGGAG ACTGGCCGGC TAAACCCAAA ATGCCGTTGA TTCCGGGACA TGAAGGTGTT GGTATCGTCG TTGCTTGTGG TCCGGATGCT ATGGTAAAAG AAGGTGATGC TGTCGGAGTT CCTTGGTTAT ATAGTGCTTG TGGATGCTGT GATTATTGTA TAACAGGCTG GGAAACGCTT TGTGAAGCCC AACAGAATGG AGGATATAGT GTAGATGGAG GATTTGCTGA ATATGTAATT GCAGATTCCC GATATGTGGG ACATCTGAAA TCTAATGTCA ATTTTCTAGA GATTGCGCCA ATTTTGTGTG CAGGTGTTAC TGTCTATAAA GGATTAAAAG AAACAGAAAC CAAACCTGGT GAATGGGTTG CGATTTCCGG AATTGGCGGA TTAGGACATG TTGCCGTACA GTATGCAAAG GCAATGGGAA TGCATGTCGC AGCTATAGAT GTTGCAGATG ATAAACTGGA GCTTGCAAAA AAGCTAGGAG CAGATCTTAC GGTAAATGCA AAAACAACCG ATCCGGGTAC CTATTTGCAT AAAGAAGTAG GTGGAATGCA TGGGGCTTTG ATAACAGCTG TTTCTCCGAT TGCCTTTAAA CAAGGAATCG ATGTGCTCAG AAGAAAAGGA ACTATTGCGC TGAATGGGCT TCCTCCGGGA TCATTTGAAT TGCCAATTTT TGAAACGGTA CTAAAGAGGA TTACAGTAAG AGGCTCTATT GTAGGGACAA GAAAAGATTT GCAGGAGGCA TTGGACTTTG CCAATGAAGG CTTGGTAAAA GCTACAGTAA CTTCAGCAAA ATTGGAAGAT ATTAATGATG TTTTTGATAA AATGAAAAAA GGCCAGATCG ACGGAAGAAT AGTTCTGGAT ATTGCAGGAA GTCAAAATTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT