SSGCID: Target Page

ElanA.10201.a

Methionine--tRNA ligase

CENTER ID:	ElanA.10201.a
ORGANISM:	Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.10201.a.B1.GE41091	Full length(ElanA.10201.a)		1	677

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.10201.a.B1.PW38260	Full length(ElanA.10201.a)		1	677

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1338011.4.peg.511
UniProt:	A0A077EEX2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTRKMITAA LPYANGPVHI GHLAGVYIPA DVYARFQRRL GKDVAFICGS DEHGIPITIR AKKEGVTPQD VVDKYHEIIK KSFADLGISF DEYSRTTSKK HYETSQDFFK TLYDKEKFTE EVSEQYFDEQ AGEFLADRYI VGTCPNCGNE NAYGDQCEKC GSTLSPSELI NPKSMLSGNV PVLKETKNWY LPLNDYENFL NEWIIEGHKD DWKPNVYGQV KSWLNDGLKP RAMTRDLNWG VPVPLPDAEG KVLYVWFDAP IGYISFTKEW AEKNGKDWKD YWQNEDSDLI HFIGKDNIVF HCIIFPAMMK AHGDYIMPKN VPAFEFLNLE NDKISTSRNW AVWAHEYVEE FPGQQDVLRY TLLSSAPETK DNNFTWKDFQ TKNNSELVGI FGNFINRVAV LIHKYYDGVI PQGDINAPEL AEINKAAKEI AEFLDKFEFR NALSALMNLA RFGNQYLQTE EPWKTIKDNP EKAAHSLFVG AQLAVALAQL SEPFMPFSSE KLLNMFNVTK TDWADLENNN VLIASGHQIN EASLLFSKIE DDVIEAQIQK LEQTKQDNKK TNPNANPMKE EITFDDFGKI DLRTATILEA EKVEKADKLL KFKVDTGVDI RTVVSGVAES FNPEELIGKQ VMILLNLAPR KIRGIESQGM FLLTTKPDGK LSFVTPDEPV ENGIEIK

NT Sequence

ATGTCTACAA GAAAGATGAT TACGGCAGCT TTGCCTTATG CAAACGGACC GGTTCATATT GGGCATTTGG CAGGTGTTTA TATTCCTGCG GATGTCTATG CGAGATTTCA GAGAAGATTA GGAAAAGATG TTGCTTTTAT CTGTGGTTCC GACGAACACG GAATTCCAAT TACTATACGT GCTAAAAAAG AAGGTGTAAC ACCTCAGGAT GTAGTAGACA AATATCACGA GATTATTAAG AAGTCTTTTG CAGATCTTGG AATCTCATTT GATGAGTATT CCAGAACGAC TTCTAAAAAA CATTATGAAA CTTCACAAGA TTTCTTCAAA ACACTTTACG ATAAAGAAAA GTTTACTGAA GAAGTTTCTG AACAATATTT TGACGAGCAG GCAGGAGAAT TCCTTGCTGA CCGTTATATC GTAGGGACAT GCCCTAACTG TGGCAACGAA AATGCTTATG GTGACCAGTG TGAGAAATGT GGCTCTACCC TTTCTCCTTC CGAGCTTATC AATCCTAAAT CTATGTTAAG TGGAAATGTT CCGGTTCTGA AAGAAACAAA AAACTGGTAT CTTCCATTAA ACGATTATGA AAACTTCCTG AACGAATGGA TTATTGAAGG TCACAAAGAT GACTGGAAGC CTAATGTATA CGGACAGGTG AAGTCTTGGC TAAACGACGG ACTGAAACCT CGTGCTATGA CGAGAGACCT TAACTGGGGA GTTCCTGTAC CATTACCGGA TGCTGAAGGA AAAGTACTAT ATGTATGGTT CGACGCTCCT ATTGGTTATA TTTCTTTTAC CAAAGAATGG GCTGAGAAAA ATGGTAAGGA CTGGAAAGAT TACTGGCAAA ATGAAGACAG CGATCTGATC CACTTCATCG GAAAGGATAA TATTGTATTC CACTGTATTA TTTTCCCTGC TATGATGAAG GCTCACGGTG ATTATATTAT GCCTAAAAAT GTACCTGCAT TCGAATTCCT GAATCTGGAA AATGATAAAA TTTCTACTTC CAGAAACTGG GCAGTATGGG CACATGAATA TGTAGAAGAA TTCCCGGGAC AACAGGATGT ATTGCGTTAT ACCCTACTTT CTTCTGCACC AGAAACTAAG GATAACAATT TTACATGGAA AGACTTCCAG ACAAAGAACA ATTCAGAGTT GGTGGGTATT TTCGGTAACT TCATCAACAG AGTTGCGGTA TTAATTCATA AATACTATGA TGGTGTTATT CCTCAGGGAG ATATTAATGC ACCAGAATTA GCTGAAATAA ACAAGGCTGC AAAAGAAATT GCTGAATTCT TAGATAAATT TGAATTCAGA AACGCTTTAT CTGCATTGAT GAATCTTGCT CGTTTCGGTA ACCAATACCT GCAAACAGAA GAGCCTTGGA AGACCATTAA GGATAATCCG GAAAAAGCGG CACATTCACT TTTCGTAGGT GCTCAATTAG CGGTTGCTTT AGCACAGCTA TCTGAACCGT TTATGCCATT CAGTTCTGAG AAGTTGTTAA ACATGTTCAA CGTTACTAAA ACAGACTGGG CAGATTTAGA AAATAATAAT GTTCTTATTG CCAGCGGACA CCAGATCAAT GAAGCATCTC TTCTTTTCTC AAAAATTGAA GATGATGTGA TTGAAGCACA AATCCAAAAA CTGGAACAGA CAAAACAAGA TAATAAAAAG ACCAACCCTA ACGCTAATCC TATGAAAGAA GAAATCACTT TTGATGACTT TGGAAAAATC GATTTAAGAA CAGCAACAAT CCTTGAAGCT GAGAAAGTAG AAAAAGCAGA CAAACTTTTA AAATTCAAAG TGGATACAGG TGTAGATATC AGAACTGTAG TTTCGGGTGT AGCTGAAAGC TTTAATCCTG AAGAATTAAT CGGAAAGCAG GTAATGATTC TTTTGAATCT TGCACCAAGA AAAATCCGTG GTATTGAATC TCAGGGGATG TTCCTTCTGA CAACAAAACC AGACGGAAAA TTGTCTTTCG TAACTCCGGA TGAACCTGTA GAAAACGGAA TTGAGATTAA A

Details for ElanA.10201.a.B1.GE41091

HARVESTED ON:	2/8/2017
SEQUENCED ON:	2/13/2017
EXPECTED MW:	78kDa
OBSERVED MW:	77kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS TRKMITAALP YANGPVHIGH LAGVYIPADV YARFQRRLGK DVAFICGSDE HGIPITIRAK KEGVTPQDVV DKYHEIIKKS FADLGISFDE YSRTTSKKHY ETSQDFFKTL YDKEKFTEEV SEQYFDEQAG EFLADRYIVG TCPNCGNENA YGDQCEKCGS TLSPSELINP KSMLSGNVPV LKETKNWYLP LNDYENFLNE WIIEGHKDDW KPNVYGQVKS WLNDGLKPRA MTRDLNWGVP VPLPDAEGKV LYVWFDAPIG YISFTKEWAE KNGKDWKDYW QNEDSDLIHF IGKDNIVFHC IIFPAMMKAH GDYIMPKNVP AFEFLNLEND KISTSRNWAV WAHEYVEEFP GQQDVLRYTL LSSAPETKDN NFTWKDFQTK NNSELVGIFG NFINRVAVLI HKYYDGVIPQ GDINAPELAE INKAAKEIAE FLDKFEFRNA LSALMNLARF GNQYLQTEEP WKTIKDNPEK AAHSLFVGAQ LAVALAQLSE PFMPFSSEKL LNMFNVTKTD WADLENNNVL IASGHQINEA SLLFSKIEDD VIEAQIQKLE QTKQDNKKTN PNANPMKEEI TFDDFGKIDL RTATILEAEK VEKADKLLKF KVDTGVDIRT VVSGVAESFN PEELIGKQVM ILLNLAPRKI RGIESQGMFL LTTKPDGKLS FVTPDEPVEN GIEIK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcta caagaaagat gattacggca gctttgcctt atgcaaacgg accggttcat attgggcatt tggcaggtgt ttatattcct gcggatgtct atgcgagatt tcagagaaga ttaggaaaag atgttgcttt tatctgtggt tccgacgaac acggaattcc aattactata cgtgctaaaa aagaaggtgt aacacctcag gatgtagtag acaaatatca cgagattatt aagaagtctt ttgcagatct tggaatctca tttgatgagt attccagaac gacttctaaa aaacattatg aaacttcaca agatttcttc aaaacacttt acgataaaga aaagtttact gaagaagttt ctgaacaata ttttgacgag caggcaggag aattccttgc tgaccgttat atcgtaggga catgccctaa ctgtggcaac gaaaatgctt atggtgacca gtgtgagaaa tgtggctcta ccctttctcc ttccgagctt atcaatccta aatctatgtt aagtggaaat gttccggttc tgaaagaaac aaaaaactgg tatcttccat taaacgatta tgaaaacttc ctgaacgaat ggattattga aggtcacaaa gatgactgga agcctaatgt atacggacag gtgaagtctt ggctaaacga cggactgaaa cctcgtgcta tgacgagaga ccttaactgg ggagttcctg taccattacc ggatgctgaa ggaaaagtac tatatgtatg gttcgacgct cctattggtt atatttcttt taccaaagaa tgggctgaga aaaatggtaa ggactggaaa gattactggc aaaatgaaga cagcgatctg atccacttca tcggaaagga taatattgta ttccactgta ttattttccc tgctatgatg aaggctcacg gtgattatat tatgcctaaa aatgtacctg cattcgaatt cctgaatctg gaaaatgata aaatttctac ttccagaaac tgggcagtat gggcacatga atatgtagaa gaattcccgg gacaacagga tgtattgcgt tataccctac tttcttctgc accagaaact aaggataaca attttacatg gaaagacttc cagacaaaga acaattcaga gttggtgggt attttcggta acttcatcaa cagagttgcg gtattaattc ataaatacta tgatggtgtt attcctcagg gagatattaa tgcaccagaa ttagctgaaa taaacaaggc tgcaaaagaa attgctgaat tcttagataa atttgaattc agaaacgctt tatctgcatt gatgaatctt gctcgtttcg gtaaccaata cctgcaaaca gaagagcctt ggaagaccat taaggataat ccggaaaaag cggcacattc acttttcgta ggtgctcaat tagcggttgc tttagcacag ctatctgaac cgtttatgcc attcagttct gagaagttgt taaacatgtt caacgttact aaaacagact gggcagattt agaaaataat aatgttctta ttgccagcgg acaccagatc aatgaagcat ctcttctttt ctcaaaaatt gaagatgatg tgattgaagc acaaatccaa aaactggaac agacaaaaca agataataaa aagaccaacc ctaacgctaa tcctatgaaa gaagaaatca cttttgatga ctttggaaaa atcgatttaa gaacagcaac aatccttgaa gctgagaaag tagaaaaagc agacaaactt ttaaaattca aagtggatac aggtgtagat atcagaactg tagtttcggg tgtagctgaa agctttaatc ctgaagaatt aatcggaaag caggtaatga ttcttttgaa tcttgcacca agaaaaatcc gtggtattga atctcagggg atgttccttc tgacaacaaa accagacgga aaattgtctt tcgtaactcc ggatgaacct gtagaaaacg gaattgagat taaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for ElanA.10201.a.B1.PW38260

PURIFICATION DATe:	4/25/2017
CONCENTRATION:	19mg/ml
OBSERVED MW:	79kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTA CAAGAAAGAT GATTACGGCA GCTTTGCCTT ATGCAAACGG ACCGGTTCAT ATTGGGCATT TGGCAGGTGT TTATATTCCT GCGGATGTCT ATGCGAGATT TCAGAGAAGA TTAGGAAAAG ATGTTGCTTT TATCTGTGGT TCCGACGAAC ACGGAATTCC AATTACTATA CGTGCTAAAA AAGAAGGTGT AACACCTCAG GATGTAGTAG ACAAATATCA CGAGATTATT AAGAAGTCTT TTGCAGATCT TGGAATCTCA TTTGATGAGT ATTCCAGAAC GACTTCTAAA AAACATTATG AAACTTCACA AGATTTCTTC AAAACACTTT ACGATAAAGA AAAGTTTACT GAAGAAGTTT CTGAACAATA TTTTGACGAG CAGGCAGGAG AATTCCTTGC TGACCGTTAT ATCGTAGGGA CATGCCCTAA CTGTGGCAAC GAAAATGCTT ATGGTGACCA GTGTGAGAAA TGTGGCTCTA CCCTTTCTCC TTCCGAGCTT ATCAATCCTA AATCTATGTT AAGTGGAAAT GTTCCGGTTC TGAAAGAAAC AAAAAACTGG TATCTTCCAT TAAACGATTA TGAAAACTTC CTGAACGAAT GGATTATTGA AGGTCACAAA GATGACTGGA AGCCTAATGT ATACGGACAG GTGAAGTCTT GGCTAAACGA CGGACTGAAA CCTCGTGCTA TGACGAGAGA CCTTAACTGG GGAGTTCCTG TACCATTACC GGATGCTGAA GGAAAAGTAC TATATGTATG GTTCGACGCT CCTATTGGTT ATATTTCTTT TACCAAAGAA TGGGCTGAGA AAAATGGTAA GGACTGGAAA GATTACTGGC AAAATGAAGA CAGCGATCTG ATCCACTTCA TCGGAAAGGA TAATATTGTA TTCCACTGTA TTATTTTCCC TGCTATGATG AAGGCTCACG GTGATTATAT TATGCCTAAA AATGTACCTG CATTCGAATT CCTGAATCTG GAAAATGATA AAATTTCTAC TTCCAGAAAC TGGGCAGTAT GGGCACATGA ATATGTAGAA GAATTCCCGG GACAACAGGA TGTATTGCGT TATACCCTAC TTTCTTCTGC ACCAGAAACT AAGGATAACA ATTTTACATG GAAAGACTTC CAGACAAAGA ACAATTCAGA GTTGGTGGGT ATTTTCGGTA ACTTCATCAA CAGAGTTGCG GTATTAATTC ATAAATACTA TGATGGTGTT ATTCCTCAGG GAGATATTAA TGCACCAGAA TTAGCTGAAA TAAACAAGGC TGCAAAAGAA ATTGCTGAAT TCTTAGATAA ATTTGAATTC AGAAACGCTT TATCTGCATT GATGAATCTT GCTCGTTTCG GTAACCAATA CCTGCAAACA GAAGAGCCTT GGAAGACCAT TAAGGATAAT CCGGAAAAAG CGGCACATTC ACTTTTCGTA GGTGCTCAAT TAGCGGTTGC TTTAGCACAG CTATCTGAAC CGTTTATGCC ATTCAGTTCT GAGAAGTTGT TAAACATGTT CAACGTTACT AAAACAGACT GGGCAGATTT AGAAAATAAT AATGTTCTTA TTGCCAGCGG ACACCAGATC AATGAAGCAT CTCTTCTTTT CTCAAAAATT GAAGATGATG TGATTGAAGC ACAAATCCAA AAACTGGAAC AGACAAAACA AGATAATAAA AAGACCAACC CTAACGCTAA TCCTATGAAA GAAGAAATCA CTTTTGATGA CTTTGGAAAA ATCGATTTAA GAACAGCAAC AATCCTTGAA GCTGAGAAAG TAGAAAAAGC AGACAAACTT TTAAAATTCA AAGTGGATAC AGGTGTAGAT ATCAGAACTG TAGTTTCGGG TGTAGCTGAA AGCTTTAATC CTGAAGAATT AATCGGAAAG CAGGTAATGA TTCTTTTGAA TCTTGCACCA AGAAAAATCC GTGGTATTGA ATCTCAGGGG ATGTTCCTTC TGACAACAAA ACCAGACGGA AAATTGTCTT TCGTAACTCC GGATGAACCT GTAGAAAACG GAATTGAGAT TAAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT