ElanA.01640.a

Glutaryl-CoA dehydrogenase

CENTER ID: ElanA.01640.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.01640.a.B1.GE40663 Full length(ElanA.01640.a) 1 392
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.01640.a.B1.PS38369 Full length(ElanA.01640.a) 1 392

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.649
UniProt: A0A077EHQ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSYYPIHTIP DYYLIDDLLT DEHKLIRQSV REWVDSFVMP KIDDAAQHHK DIPDLMKELG KIGALGPYIP EEYGGAGLDQ ISYGLIMQEL ERGDSAVRSA ASVQSSLVMF PINEFGSEEQ KKKYLPHLAS GEMIGAFGLT EPNHGSDPGS METHFKDMGD HYLLNGAKMW ITNAPLCNIA VVWAKGEDGK VRGLIVERGM EGFTTPETIN KWSLRASKTG ELVFRDVKIP KENILPNVIG LKGPLSCLNS ARYGISWGVI GAAIDCYCTA VQYSKERIQF GKPIASYQLQ QKKLAEFLTE ITKAQLLCWR LGTLKNEHRA TPAQISLAKR NNVKMAIDIA RESRQILGGM GIMGEFPMMR HAANLESVIT YEGTHDVHLL ITGNDITGIN AF
NT Sequence
ATGTCATATT ACCCAATTCA CACCATCCCC GATTATTACC TTATAGACGA TTTATTAACA GATGAACACA AACTTATCCG ACAGTCTGTC CGCGAATGGG TAGATAGTTT TGTAATGCCG AAAATTGATG ATGCAGCGCA GCATCATAAA GATATTCCGG ATCTTATGAA GGAACTAGGG AAAATCGGAG CTTTAGGACC ATATATTCCT GAGGAATACG GAGGTGCTGG ATTGGATCAG ATTTCTTACG GACTGATTAT GCAGGAGCTG GAACGTGGTG ATTCTGCGGT TCGTTCGGCA GCATCAGTAC AATCATCATT GGTTATGTTT CCTATAAATG AGTTTGGTTC GGAGGAACAA AAAAAGAAAT ACCTTCCTCA TTTGGCTAGT GGCGAGATGA TTGGAGCTTT TGGACTGACA GAACCTAATC ATGGATCGGA CCCCGGAAGT ATGGAAACTC ATTTCAAAGA CATGGGAGAC CATTACCTGC TGAACGGCGC AAAAATGTGG ATTACCAATG CTCCTTTATG CAATATTGCA GTAGTGTGGG CTAAAGGTGA AGATGGCAAA GTAAGAGGAC TTATTGTAGA GCGTGGAATG GAAGGCTTTA CAACACCAGA AACAATTAAT AAATGGTCTT TGCGCGCATC CAAAACCGGA GAATTGGTAT TCAGAGATGT AAAGATTCCT AAAGAAAATA TATTGCCAAA TGTAATTGGA CTTAAAGGTC CGTTATCTTG TCTAAACTCT GCCCGTTATG GTATTTCCTG GGGTGTTATT GGCGCTGCAA TAGATTGTTA TTGTACAGCT GTACAATATT CAAAAGAACG TATACAATTT GGTAAGCCTA TTGCTTCTTA TCAGCTTCAG CAAAAGAAAC TGGCAGAATT CCTTACTGAG ATTACCAAAG CACAGTTGTT ATGCTGGCGT CTTGGGACAC TGAAAAATGA GCACAGAGCT ACACCTGCAC AAATATCTTT GGCGAAAAGA AATAATGTAA AAATGGCAAT CGATATTGCC AGAGAATCCA GACAGATTCT TGGCGGAATG GGAATTATGG GTGAATTTCC TATGATGCGT CATGCAGCTA ACCTGGAGTC AGTGATTACC TATGAGGGAA CTCACGATGT ACACTTACTC ATTACCGGAA ATGATATTAC CGGAATAAAT GCTTTT
Details for ElanA.01640.a.B1.GE40663
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS YYPIHTIPDY YLIDDLLTDE HKLIRQSVRE WVDSFVMPKI DDAAQHHKDI PDLMKELGKI GALGPYIPEE YGGAGLDQIS YGLIMQELER GDSAVRSAAS VQSSLVMFPI NEFGSEEQKK KYLPHLASGE MIGAFGLTEP NHGSDPGSME THFKDMGDHY LLNGAKMWIT NAPLCNIAVV WAKGEDGKVR GLIVERGMEG FTTPETINKW SLRASKTGEL VFRDVKIPKE NILPNVIGLK GPLSCLNSAR YGISWGVIGA AIDCYCTAVQ YSKERIQFGK PIASYQLQQK KLAEFLTEIT KAQLLCWRLG TLKNEHRATP AQISLAKRNN VKMAIDIARE SRQILGGMGI MGEFPMMRHA ANLESVITYE GTHDVHLLIT GN
Validated NT Sequence
atggctcacc accaccacca ccatatgtca tattacccaa ttcacaccat ccccgattat taccttatag acgatttatt aacagatgaa cacaaactta tccgacagtc tgtccgcgaa tgggtagata gttttgtaat gccgaaaatt gatgatgcag cgcagcatca taaagatatt ccggatctta tgaaggaact agggaaaatc ggagctttag gaccatatat tcctgaggaa tacggaggtg ctggattgga tcagatttct tacggactga ttatgcagga gctggaacgt ggtgattctg cggttcgttc ggcagcatca gtacaatcat cattggttat gtttcctata aatgagtttg gttcggagga acaaaaaaag aaataccttc ctcatttggc tagtggcgag atgattggag cttttggact gacagaacct aatcatggat cggaccccgg aagtatggaa actcatttca aagacatggg agaccattac ctgctgaacg gcgcaaaaat gtggattacc aatgctcctt tatgcaatat tgcagtagtg tgggctaaag gtgaagatgg caaagtaaga ggacttattg tagagcgtgg aatggaaggc tttacaacac cagaaacaat taataaatgg tctttgcgcg catccaaaac cggagaattg gtattcagag atgtaaagat tcctaaagaa aatatattgc caaatgtaat tggacttaaa ggtccgttat cttgtctaaa ctctgcccgt tatggtattt cctggggtgt tattggcgct gcaatagatt gttattgtac agctgtacaa tattcaaaag aacgtataca atttggtaag cctattgctt cttatcagct tcagcaaaag aaactggcag aattccttac tgagattacc aaagcacagt tgttatgctg gcgtcttggg acactgaaaa atgagcacag agctacacct gcacaaatat ctttggcgaa aagaaataat gtaaaaatgg caatcgatat tgccagagaa tccagacaga ttcttggcgg aatgggaatt atgggtgaat ttcctatgat gcgtcatgca gctaacctgg agtcagtgat tacctatgag ggaactcacg atgtacactt actcattacc ggaaatgat
Expected Protein Sequence
MAHHHHHHMS YYPIHTIPDY YLIDDLLTDE HKLIRQSVRE WVDSFVMPKI DDAAQHHKDI PDLMKELGKI GALGPYIPEE YGGAGLDQIS YGLIMQELER GDSAVRSAAS VQSSLVMFPI NEFGSEEQKK KYLPHLASGE MIGAFGLTEP NHGSDPGSME THFKDMGDHY LLNGAKMWIT NAPLCNIAVV WAKGEDGKVR GLIVERGMEG FTTPETINKW SLRASKTGEL VFRDVKIPKE NILPNVIGLK GPLSCLNSAR YGISWGVIGA AIDCYCTAVQ YSKERIQFGK PIASYQLQQK KLAEFLTEIT KAQLLCWRLG TLKNEHRATP AQISLAKRNN VKMAIDIARE SRQILGGMGI MGEFPMMRHA ANLESVITYE GTHDVHLLIT GNDITGINAF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcat attacccaat tcacaccatc cccgattatt accttataga cgatttatta acagatgaac acaaacttat ccgacagtct gtccgcgaat gggtagatag ttttgtaatg ccgaaaattg atgatgcagc gcagcatcat aaagatattc cggatcttat gaaggaacta gggaaaatcg gagctttagg accatatatt cctgaggaat acggaggtgc tggattggat cagatttctt acggactgat tatgcaggag ctggaacgtg gtgattctgc ggttcgttcg gcagcatcag tacaatcatc attggttatg tttcctataa atgagtttgg ttcggaggaa caaaaaaaga aataccttcc tcatttggct agtggcgaga tgattggagc ttttggactg acagaaccta atcatggatc ggaccccgga agtatggaaa ctcatttcaa agacatggga gaccattacc tgctgaacgg cgcaaaaatg tggattacca atgctccttt atgcaatatt gcagtagtgt gggctaaagg tgaagatggc aaagtaagag gacttattgt agagcgtgga atggaaggct ttacaacacc agaaacaatt aataaatggt ctttgcgcgc atccaaaacc ggagaattgg tattcagaga tgtaaagatt cctaaagaaa atatattgcc aaatgtaatt ggacttaaag gtccgttatc ttgtctaaac tctgcccgtt atggtatttc ctggggtgtt attggcgctg caatagattg ttattgtaca gctgtacaat attcaaaaga acgtatacaa tttggtaagc ctattgcttc ttatcagctt cagcaaaaga aactggcaga attccttact gagattacca aagcacagtt gttatgctgg cgtcttggga cactgaaaaa tgagcacaga gctacacctg cacaaatatc tttggcgaaa agaaataatg taaaaatggc aatcgatatt gccagagaat ccagacagat tcttggcgga atgggaatta tgggtgaatt tcctatgatg cgtcatgcag ctaacctgga gtcagtgatt acctatgagg gaactcacga tgtacactta ctcattaccg gaaatgatat taccggaata aatgcttttt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for ElanA.01640.a.B1.PS38369
PURIFICATION DATe: 10/18/2017
CONCENTRATION: 70.54mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 9
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS YYPIHTIPDY YLIDDLLTDE HKLIRQSVRE WVDSFVMPKI DDAAQHHKDI PDLMKELGKI GALGPYIPEE YGGAGLDQIS YGLIMQELER GDSAVRSAAS VQSSLVMFPI NEFGSEEQKK KYLPHLASGE MIGAFGLTEP NHGSDPGSME THFKDMGDHY LLNGAKMWIT NAPLCNIAVV WAKGEDGKVR GLIVERGMEG FTTPETINKW SLRASKTGEL VFRDVKIPKE NILPNVIGLK GPLSCLNSAR YGISWGVIGA AIDCYCTAVQ YSKERIQFGK PIASYQLQQK KLAEFLTEIT KAQLLCWRLG TLKNEHRATP AQISLAKRNN VKMAIDIARE SRQILGGMGI MGEFPMMRHA ANLESVITYE GTHDVHLLIT GN
Validated NT Sequence
atggctcacc accaccacca ccatatgtca tattacccaa ttcacaccat ccccgattat taccttatag acgatttatt aacagatgaa cacaaactta tccgacagtc tgtccgcgaa tgggtagata gttttgtaat gccgaaaatt gatgatgcag cgcagcatca taaagatatt ccggatctta tgaaggaact agggaaaatc ggagctttag gaccatatat tcctgaggaa tacggaggtg ctggattgga tcagatttct tacggactga ttatgcagga gctggaacgt ggtgattctg cggttcgttc ggcagcatca gtacaatcat cattggttat gtttcctata aatgagtttg gttcggagga acaaaaaaag aaataccttc ctcatttggc tagtggcgag atgattggag cttttggact gacagaacct aatcatggat cggaccccgg aagtatggaa actcatttca aagacatggg agaccattac ctgctgaacg gcgcaaaaat gtggattacc aatgctcctt tatgcaatat tgcagtagtg tgggctaaag gtgaagatgg caaagtaaga ggacttattg tagagcgtgg aatggaaggc tttacaacac cagaaacaat taataaatgg tctttgcgcg catccaaaac cggagaattg gtattcagag atgtaaagat tcctaaagaa aatatattgc caaatgtaat tggacttaaa ggtccgttat cttgtctaaa ctctgcccgt tatggtattt cctggggtgt tattggcgct gcaatagatt gttattgtac agctgtacaa tattcaaaag aacgtataca atttggtaag cctattgctt cttatcagct tcagcaaaag aaactggcag aattccttac tgagattacc aaagcacagt tgttatgctg gcgtcttggg acactgaaaa atgagcacag agctacacct gcacaaatat ctttggcgaa aagaaataat gtaaaaatgg caatcgatat tgccagagaa tccagacaga ttcttggcgg aatgggaatt atgggtgaat ttcctatgat gcgtcatgca gctaacctgg agtcagtgat tacctatgag ggaactcacg atgtacactt actcattacc ggaaatgat
Expressed Protein Sequence
MAHHHHHHMS YYPIHTIPDY YLIDDLLTDE HKLIRQSVRE WVDSFVMPKI DDAAQHHKDI PDLMKELGKI GALGPYIPEE YGGAGLDQIS YGLIMQELER GDSAVRSAAS VQSSLVMFPI NEFGSEEQKK KYLPHLASGE MIGAFGLTEP NHGSDPGSME THFKDMGDHY LLNGAKMWIT NAPLCNIAVV WAKGEDGKVR GLIVERGMEG FTTPETINKW SLRASKTGEL VFRDVKIPKE NILPNVIGLK GPLSCLNSAR YGISWGVIGA AIDCYCTAVQ YSKERIQFGK PIASYQLQQK KLAEFLTEIT KAQLLCWRLG TLKNEHRATP AQISLAKRNN VKMAIDIARE SRQILGGMGI MGEFPMMRHA ANLESVITYE GTHDVHLLIT GNDITGINAF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAT ATTACCCAAT TCACACCATC CCCGATTATT ACCTTATAGA CGATTTATTA ACAGATGAAC ACAAACTTAT CCGACAGTCT GTCCGCGAAT GGGTAGATAG TTTTGTAATG CCGAAAATTG ATGATGCAGC GCAGCATCAT AAAGATATTC CGGATCTTAT GAAGGAACTA GGGAAAATCG GAGCTTTAGG ACCATATATT CCTGAGGAAT ACGGAGGTGC TGGATTGGAT CAGATTTCTT ACGGACTGAT TATGCAGGAG CTGGAACGTG GTGATTCTGC GGTTCGTTCG GCAGCATCAG TACAATCATC ATTGGTTATG TTTCCTATAA ATGAGTTTGG TTCGGAGGAA CAAAAAAAGA AATACCTTCC TCATTTGGCT AGTGGCGAGA TGATTGGAGC TTTTGGACTG ACAGAACCTA ATCATGGATC GGACCCCGGA AGTATGGAAA CTCATTTCAA AGACATGGGA GACCATTACC TGCTGAACGG CGCAAAAATG TGGATTACCA ATGCTCCTTT ATGCAATATT GCAGTAGTGT GGGCTAAAGG TGAAGATGGC AAAGTAAGAG GACTTATTGT AGAGCGTGGA ATGGAAGGCT TTACAACACC AGAAACAATT AATAAATGGT CTTTGCGCGC ATCCAAAACC GGAGAATTGG TATTCAGAGA TGTAAAGATT CCTAAAGAAA ATATATTGCC AAATGTAATT GGACTTAAAG GTCCGTTATC TTGTCTAAAC TCTGCCCGTT ATGGTATTTC CTGGGGTGTT ATTGGCGCTG CAATAGATTG TTATTGTACA GCTGTACAAT ATTCAAAAGA ACGTATACAA TTTGGTAAGC CTATTGCTTC TTATCAGCTT CAGCAAAAGA AACTGGCAGA ATTCCTTACT GAGATTACCA AAGCACAGTT GTTATGCTGG CGTCTTGGGA CACTGAAAAA TGAGCACAGA GCTACACCTG CACAAATATC TTTGGCGAAA AGAAATAATG TAAAAATGGC AATCGATATT GCCAGAGAAT CCAGACAGAT TCTTGGCGGA ATGGGAATTA TGGGTGAATT TCCTATGATG CGTCATGCAG CTAACCTGGA GTCAGTGATT ACCTATGAGG GAACTCACGA TGTACACTTA CTCATTACCG GAAATGATAT TACCGGAATA AATGCTTTTT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT