ElanA.01530.a
1,4-dihydroxy-2-naphthoyl-CoA synthase
| CENTER ID: | ElanA.01530.a |
| ORGANISM: | Elizabethkingia anophelis NUHP1 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ElanA.01530.a.B1.GE40660 | Full length(ElanA.01530.a) | 1 | 278 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ElanA.01530.a.B1.PW38414 | Full length(ElanA.01530.a) | 1 | 278 |
Structures
6OJM
DEPOSITED: 4/11/2019
DETERMINATION: XRay
CLONE: ElanA.01530.a.B1.GE40660
PROTEIN: ElanA.01530.a.B1.PW38414
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1338011.4.peg.2658 |
| UniProt: | A0A077EJG6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIEWKTAKEY EDITYKKCNG VARIAFNRPE IRNAFRPKTT SELYDAFYDA YEDPSIGVVL LSGEGPSPKD GGWAFCSGGD QKARGHQGYV GEDGRHRLNI LEVQRLIRFM PKVVIAVVPG WAVGGGHSLH VVCDLTLASK EHAIFKQTDA DVTSFDGGYG SAYLAKMVGQ KKAREIFFLG RNYSAQEAFE MGMVNKVVPH AELEDTAYEW AQEILAKSPT SIRMLKFAMN LTDDGMVGQQ VFAGEATRLA YMTDEAKEGR NAFLEKRKPD FGEDQWIS
NT Sequence
ATGATCGAGT GGAAAACCGC AAAAGAATAC GAAGATATTA CTTATAAAAA ATGTAATGGT GTAGCAAGAA TTGCTTTCAA CAGACCAGAA ATCCGCAACG CTTTCAGACC TAAAACGACT TCAGAATTAT ATGATGCTTT TTATGATGCA TATGAAGACC CGTCAATTGG AGTAGTACTA TTATCCGGAG AAGGACCAAG TCCAAAAGAC GGAGGATGGG CTTTCTGTAG TGGTGGAGAT CAAAAAGCAC GTGGTCACCA GGGTTATGTT GGAGAAGACG GAAGACACCG TTTGAATATT CTGGAAGTAC AACGTTTAAT CCGTTTTATG CCTAAAGTCG TTATTGCGGT AGTTCCGGGA TGGGCTGTAG GTGGAGGACA CTCACTACAC GTTGTATGTG ATCTTACTTT AGCAAGTAAA GAACATGCAA TTTTCAAACA AACAGATGCT GATGTTACCA GTTTCGATGG TGGTTATGGT TCAGCATATC TGGCTAAAAT GGTTGGGCAG AAAAAAGCGC GTGAAATATT TTTCTTAGGA AGAAATTATT CTGCACAGGA AGCTTTTGAA ATGGGAATGG TAAATAAAGT TGTTCCTCAT GCCGAATTAG AAGACACTGC TTATGAATGG GCACAGGAAA TTTTAGCGAA ATCACCAACT TCTATTCGTA TGCTGAAATT TGCAATGAAC CTTACTGATG ATGGTATGGT TGGGCAGCAA GTTTTTGCAG GTGAGGCTAC CCGTTTGGCT TACATGACAG ATGAGGCTAA AGAAGGAAGA AATGCTTTCC TTGAGAAAAG AAAACCAGAC TTTGGAGAAG ATCAATGGAT CTCT
Details for ElanA.01530.a.B1.GE40660
| HARVESTED ON: | 11/8/2016 |
| SEQUENCED ON: | 11/8/2016 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMI EWKTAKEYED ITYKKCNGVA RIAFNRPEIR NAFRPKTTSE LYDAFYDAYE DPSIGVVLLS GEGPSPKDGG WAFCSGGDQK ARGHQGYVGE DGRHRLNILE VQRLIRFMPK VVIAVVPGWA VGGGHSLHVV CDLTLASKEH AIFKQTDADV TSFDGGYGSA YLAKMVGQKK AREIFFLGRN YSAQEAFEMG MVNKVVPHAE LEDTAYEWAQ EILAKSPTSI RMLKFAMNLT DDGMVGQQVF AGEATRLAYM TDEAKEGRNA FLEKRKPDFX EDQWIS
Validated NT Sequence
atggctcacc accaccacca ccatatgatc gagtggaaaa ccgcaaaaga atacgaagat attacttata aaaaatgtaa tggtgtagca agaattgctt tcaacagacc agaaatccgc aacgctttca gacctaaaac gacttcagaa ttatatgatg ctttttatga tgcatatgaa gacccgtcaa ttggagtagt actattatcc ggagaaggac caagtccaaa agacggagga tgggctttct gtagtggtgg agatcaaaaa gcacgtggtc accagggtta tgttggagaa gacggaagac accgtttgaa tattctggaa gtacaacgtt taatccgttt tatgcctaaa gtcgttattg cggtagttcc gggatgggct gtaggtggag gacactcact acacgttgta tgtgatctta ctttagcaag taaagaacat gcaattttca aacaaacaga tgctgatgtt accagtttcg atggtggtta tggttcagca tatctggcta aaatggttgg gcagaaaaaa gcgcgtgaaa tatttttctt aggaagaaat tattctgcac aggaagcttt tgaaatggga atggtaaata aagttgttcc tcatgccgaa ttagaagaca ctgcttatga atgggcacag gaaattttag cgaaatcacc aacttctatt cgtatgctga aatttgcaat gaaccttact gatgatggta tggttgggca gcaagttttt gcaggtgagg ctacccgttt ggcttacatg acagatgagg ctaaagaagg aagaaatgct ttccttgaga aaagaaaacc agactttnnn gaagatcaat ggatctctta agtgagtaag at
Expected Protein Sequence
MAHHHHHHMI EWKTAKEYED ITYKKCNGVA RIAFNRPEIR NAFRPKTTSE LYDAFYDAYE DPSIGVVLLS GEGPSPKDGG WAFCSGGDQK ARGHQGYVGE DGRHRLNILE VQRLIRFMPK VVIAVVPGWA VGGGHSLHVV CDLTLASKEH AIFKQTDADV TSFDGGYGSA YLAKMVGQKK AREIFFLGRN YSAQEAFEMG MVNKVVPHAE LEDTAYEWAQ EILAKSPTSI RMLKFAMNLT DDGMVGQQVF AGEATRLAYM TDEAKEGRNA FLEKRKPDFG EDQWIS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcg agtggaaaac cgcaaaagaa tacgaagata ttacttataa aaaatgtaat ggtgtagcaa gaattgcttt caacagacca gaaatccgca acgctttcag acctaaaacg acttcagaat tatatgatgc tttttatgat gcatatgaag acccgtcaat tggagtagta ctattatccg gagaaggacc aagtccaaaa gacggaggat gggctttctg tagtggtgga gatcaaaaag cacgtggtca ccagggttat gttggagaag acggaagaca ccgtttgaat attctggaag tacaacgttt aatccgtttt atgcctaaag tcgttattgc ggtagttccg ggatgggctg taggtggagg acactcacta cacgttgtat gtgatcttac tttagcaagt aaagaacatg caattttcaa acaaacagat gctgatgtta ccagtttcga tggtggttat ggttcagcat atctggctaa aatggttggg cagaaaaaag cgcgtgaaat atttttctta ggaagaaatt attctgcaca ggaagctttt gaaatgggaa tggtaaataa agttgttcct catgccgaat tagaagacac tgcttatgaa tgggcacagg aaattttagc gaaatcacca acttctattc gtatgctgaa atttgcaatg aaccttactg atgatggtat ggttgggcag caagtttttg caggtgaggc tacccgtttg gcttacatga cagatgaggc taaagaagga agaaatgctt tccttgagaa aagaaaacca gactttggag aagatcaatg gatctcttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for ElanA.01530.a.B1.PW38414
| PURIFICATION DATe: | 1/16/2018 |
| CONCENTRATION: | 40.17mg/ml |
| OBSERVED MW: | 30kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI EWKTAKEYED ITYKKCNGVA RIAFNRPEIR NAFRPKTTSE LYDAFYDAYE DPSIGVVLLS GEGPSPKDGG WAFCSGGDQK ARGHQGYVGE DGRHRLNILE VQRLIRFMPK VVIAVVPGWA VGGGHSLHVV CDLTLASKEH AIFKQTDADV TSFDGGYGSA YLAKMVGQKK AREIFFLGRN YSAQEAFEMG MVNKVVPHAE LEDTAYEWAQ EILAKSPTSI RMLKFAMNLT DDGMVGQQVF AGEATRLAYM TDEAKEGRNA FLEKRKPDFX EDQWIS
Validated NT Sequence
atggctcacc accaccacca ccatatgatc gagtggaaaa ccgcaaaaga atacgaagat attacttata aaaaatgtaa tggtgtagca agaattgctt tcaacagacc agaaatccgc aacgctttca gacctaaaac gacttcagaa ttatatgatg ctttttatga tgcatatgaa gacccgtcaa ttggagtagt actattatcc ggagaaggac caagtccaaa agacggagga tgggctttct gtagtggtgg agatcaaaaa gcacgtggtc accagggtta tgttggagaa gacggaagac accgtttgaa tattctggaa gtacaacgtt taatccgttt tatgcctaaa gtcgttattg cggtagttcc gggatgggct gtaggtggag gacactcact acacgttgta tgtgatctta ctttagcaag taaagaacat gcaattttca aacaaacaga tgctgatgtt accagtttcg atggtggtta tggttcagca tatctggcta aaatggttgg gcagaaaaaa gcgcgtgaaa tatttttctt aggaagaaat tattctgcac aggaagcttt tgaaatggga atggtaaata aagttgttcc tcatgccgaa ttagaagaca ctgcttatga atgggcacag gaaattttag cgaaatcacc aacttctatt cgtatgctga aatttgcaat gaaccttact gatgatggta tggttgggca gcaagttttt gcaggtgagg ctacccgttt ggcttacatg acagatgagg ctaaagaagg aagaaatgct ttccttgaga aaagaaaacc agactttnnn gaagatcaat ggatctctta agtgagtaag at
Expressed Protein Sequence
MAHHHHHHMI EWKTAKEYED ITYKKCNGVA RIAFNRPEIR NAFRPKTTSE LYDAFYDAYE DPSIGVVLLS GEGPSPKDGG WAFCSGGDQK ARGHQGYVGE DGRHRLNILE VQRLIRFMPK VVIAVVPGWA VGGGHSLHVV CDLTLASKEH AIFKQTDADV TSFDGGYGSA YLAKMVGQKK AREIFFLGRN YSAQEAFEMG MVNKVVPHAE LEDTAYEWAQ EILAKSPTSI RMLKFAMNLT DDGMVGQQVF AGEATRLAYM TDEAKEGRNA FLEKRKPDFG EDQWIS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCG AGTGGAAAAC CGCAAAAGAA TACGAAGATA TTACTTATAA AAAATGTAAT GGTGTAGCAA GAATTGCTTT CAACAGACCA GAAATCCGCA ACGCTTTCAG ACCTAAAACG ACTTCAGAAT TATATGATGC TTTTTATGAT GCATATGAAG ACCCGTCAAT TGGAGTAGTA CTATTATCCG GAGAAGGACC AAGTCCAAAA GACGGAGGAT GGGCTTTCTG TAGTGGTGGA GATCAAAAAG CACGTGGTCA CCAGGGTTAT GTTGGAGAAG ACGGAAGACA CCGTTTGAAT ATTCTGGAAG TACAACGTTT AATCCGTTTT ATGCCTAAAG TCGTTATTGC GGTAGTTCCG GGATGGGCTG TAGGTGGAGG ACACTCACTA CACGTTGTAT GTGATCTTAC TTTAGCAAGT AAAGAACATG CAATTTTCAA ACAAACAGAT GCTGATGTTA CCAGTTTCGA TGGTGGTTAT GGTTCAGCAT ATCTGGCTAA AATGGTTGGG CAGAAAAAAG CGCGTGAAAT ATTTTTCTTA GGAAGAAATT ATTCTGCACA GGAAGCTTTT GAAATGGGAA TGGTAAATAA AGTTGTTCCT CATGCCGAAT TAGAAGACAC TGCTTATGAA TGGGCACAGG AAATTTTAGC GAAATCACCA ACTTCTATTC GTATGCTGAA ATTTGCAATG AACCTTACTG ATGATGGTAT GGTTGGGCAG CAAGTTTTTG CAGGTGAGGC TACCCGTTTG GCTTACATGA CAGATGAGGC TAAAGAAGGA AGAAATGCTT TCCTTGAGAA AAGAAAACCA GACTTTGGAG AAGATCAATG GATCTCTTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT