ElanA.01461.a

Dihydroorotate dehydrogenase

CENTER ID: ElanA.01461.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.01461.a.B1.GE40659 Full length(ElanA.01461.a) 1 311
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.01461.a.B1.PS38387 Full length(ElanA.01461.a) 1 311

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.1539
UniProt: A0A077E993

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLTSRIANY EFENPFMNAS GVMCYDEMEL DQLLRSSAGA FVTKSATPDF REGNPSPRYV DVPLGSINSM GLPNKGFDFY LDFSLNFQNE NPGKINFISI AGMSMEDNLE MLQKINDSDF KGITELNLSC PNVPGKPQVG YDFERTEEVL TKAFEFFQKP IGVKLPPYFD IAHFDQMAEI LNKFPLQYVN CINSIGNGLY IDVDHEEVVI KPKEGFGGIG GAYVKPTALA NVRAFYTRLN SDIAVIGCGG VENGKDAFEH LLCGAQMVQV GTQLMKEGAG AFDRILEELK AIMQEKGYTS IEDFRGKLKS L
NT Sequence
ATGAATCTTA CCTCCCGAAT TGCCAATTAC GAATTTGAAA ATCCATTCAT GAACGCTTCC GGCGTAATGT GCTATGACGA AATGGAACTG GATCAGTTAC TGAGATCTTC TGCAGGTGCT TTTGTAACTA AAAGTGCTAC GCCAGATTTC AGAGAAGGGA ATCCTAGCCC AAGATATGTA GATGTACCTT TAGGAAGCAT AAACTCCATG GGATTGCCTA ATAAAGGTTT TGATTTCTAT CTGGATTTTT CTCTTAACTT TCAGAATGAA AATCCGGGAA AAATCAATTT TATATCGATT GCCGGAATGT CTATGGAAGA CAATCTGGAA ATGTTACAAA AAATAAATGA TTCCGATTTT AAAGGAATTA CCGAATTAAA TCTTTCTTGC CCCAACGTAC CAGGGAAACC ACAGGTAGGA TATGATTTCG AAAGAACTGA AGAAGTTTTA ACAAAAGCTT TTGAATTCTT CCAGAAACCA ATTGGTGTAA AACTTCCGCC ATACTTCGAT ATTGCGCATT TCGATCAGAT GGCTGAAATC TTAAATAAAT TCCCGCTTCA GTACGTTAAC TGCATCAACT CTATCGGAAA CGGACTTTAT ATAGATGTAG ATCACGAAGA AGTTGTAATT AAGCCAAAAG AGGGATTCGG AGGAATTGGT GGCGCTTATG TAAAACCAAC TGCACTGGCT AACGTAAGAG CTTTCTACAC AAGACTAAAC AGTGATATTG CTGTAATCGG ATGTGGGGGT GTAGAAAACG GAAAAGATGC ATTTGAACAC TTACTTTGTG GCGCACAGAT GGTACAGGTA GGAACCCAAC TGATGAAAGA AGGGGCTGGC GCTTTCGACA GAATATTGGA AGAGCTAAAA GCTATTATGC AAGAAAAAGG CTATACAAGC ATAGAAGATT TCAGAGGTAA GCTGAAAAGT TTA
Details for ElanA.01461.a.B1.GE40659
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN LTSRIANYEF ENPFMNASGV MCYDEMELDQ LLRSSAGAFV TKSATPDFRE GNPSPRYVDV PLGSINSMGL PNKGFDFYLD FSLNFQNENP GKINFISIAG MSMEDNLEML QKINDSDFKG ITELNLSCPN VPGKPQVGYD FERTEEVLTK AFEFFQKPIG VKLPPYFDIA HFDQMAEILN KFPLQYVNCI NSIGNGLYID VDHEEVVIKP KEGFGGIGGA YVKPTALANV RAFYTRLNSD IAVIGCGGVE NGKDAFEHLL CGAQMVQVGT QLMKEGAGAF DRILEELKAI MQEKGYTSIE DFXXXLKSL
Validated NT Sequence
atggctcacc accaccacca ccatatgaat cttacctccc gaattgccaa ttacgaattt gaaaatccat tcatgaacgc ttccggcgta atgtgctatg acgaaatgga actggatcag ttactgagat cttctgcagg tgcttttgta actaaaagtg ctacgccaga tttcagagaa gggaatccta gcccaagata tgtagatgta cctttaggaa gcataaactc catgggattg cctaataaag gttttgattt ctatctggat ttttctctta actttcagaa tgaaaatccg ggaaaaatca attttatatc gattgccgga atgtctatgg aagacaatct ggaaatgtta caaaaaataa atgattccga ttttaaagga attaccgaat taaatctttc ttgccccaac gtaccaggga aaccacaggt aggatatgat ttcgaaagaa ctgaagaagt tttaacaaaa gcttttgaat tcttccagaa accaattggt gtaaaacttc cgccatactt cgatattgcg catttcgatc agatggctga aatcttaaat aaattcccgc ttcagtacgt taactgcatc aactctatcg gaaacggact ttatatagat gtagatcacg aagaagttgt aattaagcca aaagagggat tcggaggaat tggtggcgct tatgtaaaac caactgcact ggctaacgta agagctttct acacaagact aaacagtgat attgctgtaa tcggatgtgg gggtgtagaa aacggaaaag atgcatttga acacttactt tgtggcgcac agatggtaca ggtaggaacc caactgatga aagaaggggc tggcgctttc gacagaatat tggaagagct aaaagctatt atgcaagaaa aaggctatac aagcatagaa gatttcnnnn nnnnnctgaa aagtttataa gtgagtaa
Expected Protein Sequence
MAHHHHHHMN LTSRIANYEF ENPFMNASGV MCYDEMELDQ LLRSSAGAFV TKSATPDFRE GNPSPRYVDV PLGSINSMGL PNKGFDFYLD FSLNFQNENP GKINFISIAG MSMEDNLEML QKINDSDFKG ITELNLSCPN VPGKPQVGYD FERTEEVLTK AFEFFQKPIG VKLPPYFDIA HFDQMAEILN KFPLQYVNCI NSIGNGLYID VDHEEVVIKP KEGFGGIGGA YVKPTALANV RAFYTRLNSD IAVIGCGGVE NGKDAFEHLL CGAQMVQVGT QLMKEGAGAF DRILEELKAI MQEKGYTSIE DFRGKLKSL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc ttacctcccg aattgccaat tacgaatttg aaaatccatt catgaacgct tccggcgtaa tgtgctatga cgaaatggaa ctggatcagt tactgagatc ttctgcaggt gcttttgtaa ctaaaagtgc tacgccagat ttcagagaag ggaatcctag cccaagatat gtagatgtac ctttaggaag cataaactcc atgggattgc ctaataaagg ttttgatttc tatctggatt tttctcttaa ctttcagaat gaaaatccgg gaaaaatcaa ttttatatcg attgccggaa tgtctatgga agacaatctg gaaatgttac aaaaaataaa tgattccgat tttaaaggaa ttaccgaatt aaatctttct tgccccaacg taccagggaa accacaggta ggatatgatt tcgaaagaac tgaagaagtt ttaacaaaag cttttgaatt cttccagaaa ccaattggtg taaaacttcc gccatacttc gatattgcgc atttcgatca gatggctgaa atcttaaata aattcccgct tcagtacgtt aactgcatca actctatcgg aaacggactt tatatagatg tagatcacga agaagttgta attaagccaa aagagggatt cggaggaatt ggtggcgctt atgtaaaacc aactgcactg gctaacgtaa gagctttcta cacaagacta aacagtgata ttgctgtaat cggatgtggg ggtgtagaaa acggaaaaga tgcatttgaa cacttacttt gtggcgcaca gatggtacag gtaggaaccc aactgatgaa agaaggggct ggcgctttcg acagaatatt ggaagagcta aaagctatta tgcaagaaaa aggctataca agcatagaag atttcagagg taagctgaaa agtttatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for ElanA.01461.a.B1.PS38387
PURIFICATION DATe: 11/21/2017
CONCENTRATION: 85.6mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN LTSRIANYEF ENPFMNASGV MCYDEMELDQ LLRSSAGAFV TKSATPDFRE GNPSPRYVDV PLGSINSMGL PNKGFDFYLD FSLNFQNENP GKINFISIAG MSMEDNLEML QKINDSDFKG ITELNLSCPN VPGKPQVGYD FERTEEVLTK AFEFFQKPIG VKLPPYFDIA HFDQMAEILN KFPLQYVNCI NSIGNGLYID VDHEEVVIKP KEGFGGIGGA YVKPTALANV RAFYTRLNSD IAVIGCGGVE NGKDAFEHLL CGAQMVQVGT QLMKEGAGAF DRILEELKAI MQEKGYTSIE DFXXXLKSL
Validated NT Sequence
atggctcacc accaccacca ccatatgaat cttacctccc gaattgccaa ttacgaattt gaaaatccat tcatgaacgc ttccggcgta atgtgctatg acgaaatgga actggatcag ttactgagat cttctgcagg tgcttttgta actaaaagtg ctacgccaga tttcagagaa gggaatccta gcccaagata tgtagatgta cctttaggaa gcataaactc catgggattg cctaataaag gttttgattt ctatctggat ttttctctta actttcagaa tgaaaatccg ggaaaaatca attttatatc gattgccgga atgtctatgg aagacaatct ggaaatgtta caaaaaataa atgattccga ttttaaagga attaccgaat taaatctttc ttgccccaac gtaccaggga aaccacaggt aggatatgat ttcgaaagaa ctgaagaagt tttaacaaaa gcttttgaat tcttccagaa accaattggt gtaaaacttc cgccatactt cgatattgcg catttcgatc agatggctga aatcttaaat aaattcccgc ttcagtacgt taactgcatc aactctatcg gaaacggact ttatatagat gtagatcacg aagaagttgt aattaagcca aaagagggat tcggaggaat tggtggcgct tatgtaaaac caactgcact ggctaacgta agagctttct acacaagact aaacagtgat attgctgtaa tcggatgtgg gggtgtagaa aacggaaaag atgcatttga acacttactt tgtggcgcac agatggtaca ggtaggaacc caactgatga aagaaggggc tggcgctttc gacagaatat tggaagagct aaaagctatt atgcaagaaa aaggctatac aagcatagaa gatttcnnnn nnnnnctgaa aagtttataa gtgagtaa
Expressed Protein Sequence
MAHHHHHHMN LTSRIANYEF ENPFMNASGV MCYDEMELDQ LLRSSAGAFV TKSATPDFRE GNPSPRYVDV PLGSINSMGL PNKGFDFYLD FSLNFQNENP GKINFISIAG MSMEDNLEML QKINDSDFKG ITELNLSCPN VPGKPQVGYD FERTEEVLTK AFEFFQKPIG VKLPPYFDIA HFDQMAEILN KFPLQYVNCI NSIGNGLYID VDHEEVVIKP KEGFGGIGGA YVKPTALANV RAFYTRLNSD IAVIGCGGVE NGKDAFEHLL CGAQMVQVGT QLMKEGAGAF DRILEELKAI MQEKGYTSIE DFRGKLKSL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATC TTACCTCCCG AATTGCCAAT TACGAATTTG AAAATCCATT CATGAACGCT TCCGGCGTAA TGTGCTATGA CGAAATGGAA CTGGATCAGT TACTGAGATC TTCTGCAGGT GCTTTTGTAA CTAAAAGTGC TACGCCAGAT TTCAGAGAAG GGAATCCTAG CCCAAGATAT GTAGATGTAC CTTTAGGAAG CATAAACTCC ATGGGATTGC CTAATAAAGG TTTTGATTTC TATCTGGATT TTTCTCTTAA CTTTCAGAAT GAAAATCCGG GAAAAATCAA TTTTATATCG ATTGCCGGAA TGTCTATGGA AGACAATCTG GAAATGTTAC AAAAAATAAA TGATTCCGAT TTTAAAGGAA TTACCGAATT AAATCTTTCT TGCCCCAACG TACCAGGGAA ACCACAGGTA GGATATGATT TCGAAAGAAC TGAAGAAGTT TTAACAAAAG CTTTTGAATT CTTCCAGAAA CCAATTGGTG TAAAACTTCC GCCATACTTC GATATTGCGC ATTTCGATCA GATGGCTGAA ATCTTAAATA AATTCCCGCT TCAGTACGTT AACTGCATCA ACTCTATCGG AAACGGACTT TATATAGATG TAGATCACGA AGAAGTTGTA ATTAAGCCAA AAGAGGGATT CGGAGGAATT GGTGGCGCTT ATGTAAAACC AACTGCACTG GCTAACGTAA GAGCTTTCTA CACAAGACTA AACAGTGATA TTGCTGTAAT CGGATGTGGG GGTGTAGAAA ACGGAAAAGA TGCATTTGAA CACTTACTTT GTGGCGCACA GATGGTACAG GTAGGAACCC AACTGATGAA AGAAGGGGCT GGCGCTTTCG ACAGAATATT GGAAGAGCTA AAAGCTATTA TGCAAGAAAA AGGCTATACA AGCATAGAAG ATTTCAGAGG TAAGCTGAAA AGTTTATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T