SSGCID: Target Page

ElanA.01402.a

Fructose-bisphosphate aldolase class II

CENTER ID:	ElanA.01402.a
ORGANISM:	Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.01402.a.B1.GE40657	Full length(ElanA.01402.a)		1	356

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.01402.a.B1.PW38374	Full length(ElanA.01402.a)		1	356

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1338011.4.peg.257
UniProt:	A0A077EQG5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKMFPAGVA TGSMVTDIFQ YAKENKFALP AVNVIGSSNI NATMETAAKL NAPVIIQFSN GGAIYNAGKG LSNEGQRAAI LGAIAGAQHI HTLAEAYGAT VILHTDHCAK KLLPWIDGLL DASEAFYKQN GKSLYSSHML DLSEEPLEEN LEISAKYFER MAKMQMTLEV EIGVTGGEED GVDNSGVDNS LLYTQPEDVA YTYEKLKAIS DNFTIAAAFG NVHGVYKPGN VKLTPKILDN SQKYVQEKFG TADKPINFVF HGGSGSTLEE IREAIGYGVI KMNIDTDLQF AYAEGIRDYM NEKIEYLRHQ IGNPEGADAP NKKFYDPRVW VRKGEETFSK RLVQAFEDLN NIDTLK

NT Sequence

ATGAGCAAAA TGTTTCCAGC TGGCGTGGCC ACAGGTTCAA TGGTTACTGA CATTTTTCAG TATGCAAAAG AAAACAAATT TGCCTTACCG GCAGTAAATG TTATCGGTTC CAGCAACATC AATGCAACAA TGGAAACAGC CGCTAAACTT AATGCACCGG TTATTATTCA GTTTTCCAAT GGTGGAGCAA TCTACAATGC AGGAAAAGGA TTATCTAACG AGGGACAGAG AGCTGCAATA CTTGGAGCTA TTGCCGGAGC ACAACATATC CACACATTAG CAGAAGCTTA TGGAGCAACT GTTATTTTAC ACACTGACCA CTGTGCAAAA AAATTACTTC CTTGGATCGA CGGACTTTTA GATGCCAGTG AAGCTTTCTA TAAGCAAAAC GGCAAATCTT TATATTCTTC TCACATGCTT GACCTTTCTG AAGAGCCTTT AGAAGAGAAC CTGGAGATTA GTGCAAAATA TTTCGAGAGA ATGGCAAAAA TGCAGATGAC TTTGGAAGTA GAGATTGGTG TAACAGGAGG GGAAGAAGAT GGTGTAGATA ACTCTGGTGT AGACAATTCA TTGCTATATA CTCAGCCAGA AGACGTAGCT TACACATACG AAAAGCTAAA AGCAATTTCT GATAACTTTA CTATTGCAGC CGCTTTTGGT AACGTTCACG GAGTATACAA GCCAGGTAAC GTAAAACTTA CCCCAAAAAT TTTAGATAAT TCGCAGAAAT ATGTTCAGGA GAAATTCGGA ACAGCTGATA AACCAATAAA CTTTGTATTC CACGGAGGTT CAGGTTCAAC ACTTGAAGAA ATTCGTGAAG CAATTGGTTA TGGTGTTATC AAAATGAATA TTGATACTGA CCTTCAGTTT GCTTATGCAG AAGGAATCAG AGACTATATG AATGAAAAAA TAGAATACCT GAGACATCAG ATTGGTAACC CGGAAGGAGC AGATGCACCA AACAAGAAAT TCTACGACCC GAGAGTATGG GTTAGAAAAG GAGAGGAAAC ATTCTCTAAA CGTCTTGTTC AGGCTTTCGA GGATCTAAAT AATATTGATA CCCTTAAA

Details for ElanA.01402.a.B1.GE40657

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	40kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMX KMFPAGVATG SMVTDIFQYA KENKFALPAV NVIGSSNINA TMETAAKLNA PVIIQFSNGG AIYNAGKGLS NEGQRAAILG AIAGAQHIHT LAEAYGATVI LHTDHCAKKL LPWIDGLLDA SEAFYKQNGK SLYSSHMLDL SEEPLEENLE ISAKYFERMA KMQMTLEVEI GVTGGEEDGV DNSGVDNSLL YTQPEDVAYT YEKLKAISDN FTIAAAFGNV HGVYKPGNVK LTPKILDNSQ KYVQEKFGTA DKPINFVFHG GSGSTLEEIR EAIGYGVIKM NIDTDLQFAY AEGIRDYMNE KIEYLRHQIG NPEGADAPNK KFYDPRVWVR KGEETFSKRL VQAFEDL

Validated NT Sequence

atggctcacc accaccacca ccatatgnnc aaaatgtttc cagctggcgt ggccacaggt tcaatggtta ctgacatttt tcagtatgca aaagaaaaca aatttgcctt accggcagta aatgttatcg gttccagcaa catcaatgca acaatggaaa cagccgctaa acttaatgca ccggttatta ttcagttttc caatggtgga gcaatctaca atgcaggaaa aggattatct aacgagggac agagagctgc aatacttgga gctattgccg gagcacaaca tatccacaca ttagcagaag cttatggagc aactgttatt ttacacactg accactgtgc aaaaaaatta cttccttgga tcgacggact tttagatgcc agtgaagctt tctataagca aaacggcaaa tctttatatt cttctcacat gcttgacctt tctgaagagc ctttagaaga gaacctggag attagtgcaa aatatttcga gagaatggca aaaatgcaga tgactttgga agtagagatt ggtgtaacag gaggggaaga agatggtgta gataactctg gtgtagacaa ttcattgcta tatactcagc cagaagacgt agcttacaca tacgaaaagc taaaagcaat ttctgataac tttactattg cagccgcttt tggtaacgtt cacggagtat acaagccagg taacgtaaaa cttaccccaa aaattttaga taattcgcag aaatatgttc aggagaaatt cggaacagct gataaaccaa taaactttgt attccacgga ggttcaggtt caacacttga agaaattcgt gaagcaattg gttatggtgt tatcaaaatg aatattgata ctgaccttca gtttgcttat gcagaaggaa tcagagacta tatgaatgaa aaaatagaat acctgagaca tcagattggt aacccggaag gagcagatgc accaaacaag aaattctacg acccgagagt atgggttaga aaaggagagg aaacattctc taaacgtctt gttcaggctt tcgaggatct aa

Expected Protein Sequence

MAHHHHHHMS KMFPAGVATG SMVTDIFQYA KENKFALPAV NVIGSSNINA TMETAAKLNA PVIIQFSNGG AIYNAGKGLS NEGQRAAILG AIAGAQHIHT LAEAYGATVI LHTDHCAKKL LPWIDGLLDA SEAFYKQNGK SLYSSHMLDL SEEPLEENLE ISAKYFERMA KMQMTLEVEI GVTGGEEDGV DNSGVDNSLL YTQPEDVAYT YEKLKAISDN FTIAAAFGNV HGVYKPGNVK LTPKILDNSQ KYVQEKFGTA DKPINFVFHG GSGSTLEEIR EAIGYGVIKM NIDTDLQFAY AEGIRDYMNE KIEYLRHQIG NPEGADAPNK KFYDPRVWVR KGEETFSKRL VQAFEDLNNI DTLK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca aaatgtttcc agctggcgtg gccacaggtt caatggttac tgacattttt cagtatgcaa aagaaaacaa atttgcctta ccggcagtaa atgttatcgg ttccagcaac atcaatgcaa caatggaaac agccgctaaa cttaatgcac cggttattat tcagttttcc aatggtggag caatctacaa tgcaggaaaa ggattatcta acgagggaca gagagctgca atacttggag ctattgccgg agcacaacat atccacacat tagcagaagc ttatggagca actgttattt tacacactga ccactgtgca aaaaaattac ttccttggat cgacggactt ttagatgcca gtgaagcttt ctataagcaa aacggcaaat ctttatattc ttctcacatg cttgaccttt ctgaagagcc tttagaagag aacctggaga ttagtgcaaa atatttcgag agaatggcaa aaatgcagat gactttggaa gtagagattg gtgtaacagg aggggaagaa gatggtgtag ataactctgg tgtagacaat tcattgctat atactcagcc agaagacgta gcttacacat acgaaaagct aaaagcaatt tctgataact ttactattgc agccgctttt ggtaacgttc acggagtata caagccaggt aacgtaaaac ttaccccaaa aattttagat aattcgcaga aatatgttca ggagaaattc ggaacagctg ataaaccaat aaactttgta ttccacggag gttcaggttc aacacttgaa gaaattcgtg aagcaattgg ttatggtgtt atcaaaatga atattgatac tgaccttcag tttgcttatg cagaaggaat cagagactat atgaatgaaa aaatagaata cctgagacat cagattggta acccggaagg agcagatgca ccaaacaaga aattctacga cccgagagta tgggttagaa aaggagagga aacattctct aaacgtcttg ttcaggcttt cgaggatcta aataatattg atacccttaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for ElanA.01402.a.B1.PW38374

PURIFICATION DATe:	10/17/2017
CONCENTRATION:	43.14mg/ml
OBSERVED MW:	40kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

IMAC->SEC

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA AAATGTTTCC AGCTGGCGTG GCCACAGGTT CAATGGTTAC TGACATTTTT CAGTATGCAA AAGAAAACAA ATTTGCCTTA CCGGCAGTAA ATGTTATCGG TTCCAGCAAC ATCAATGCAA CAATGGAAAC AGCCGCTAAA CTTAATGCAC CGGTTATTAT TCAGTTTTCC AATGGTGGAG CAATCTACAA TGCAGGAAAA GGATTATCTA ACGAGGGACA GAGAGCTGCA ATACTTGGAG CTATTGCCGG AGCACAACAT ATCCACACAT TAGCAGAAGC TTATGGAGCA ACTGTTATTT TACACACTGA CCACTGTGCA AAAAAATTAC TTCCTTGGAT CGACGGACTT TTAGATGCCA GTGAAGCTTT CTATAAGCAA AACGGCAAAT CTTTATATTC TTCTCACATG CTTGACCTTT CTGAAGAGCC TTTAGAAGAG AACCTGGAGA TTAGTGCAAA ATATTTCGAG AGAATGGCAA AAATGCAGAT GACTTTGGAA GTAGAGATTG GTGTAACAGG AGGGGAAGAA GATGGTGTAG ATAACTCTGG TGTAGACAAT TCATTGCTAT ATACTCAGCC AGAAGACGTA GCTTACACAT ACGAAAAGCT AAAAGCAATT TCTGATAACT TTACTATTGC AGCCGCTTTT GGTAACGTTC ACGGAGTATA CAAGCCAGGT AACGTAAAAC TTACCCCAAA AATTTTAGAT AATTCGCAGA AATATGTTCA GGAGAAATTC GGAACAGCTG ATAAACCAAT AAACTTTGTA TTCCACGGAG GTTCAGGTTC AACACTTGAA GAAATTCGTG AAGCAATTGG TTATGGTGTT ATCAAAATGA ATATTGATAC TGACCTTCAG TTTGCTTATG CAGAAGGAAT CAGAGACTAT ATGAATGAAA AAATAGAATA CCTGAGACAT CAGATTGGTA ACCCGGAAGG AGCAGATGCA CCAAACAAGA AATTCTACGA CCCGAGAGTA TGGGTTAGAA AAGGAGAGGA AACATTCTCT AAACGTCTTG TTCAGGCTTT CGAGGATCTA AATAATATTG ATACCCTTAA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT