ElanA.01348.a
Glutamate--tRNA ligase
| CENTER ID: | ElanA.01348.a |
| ORGANISM: | Elizabethkingia anophelis NUHP1 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ElanA.01348.a.B1.GE41090 | Full length(ElanA.01348.a) | 1 | 503 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ElanA.01348.a.B1.PW38250 | Full length(ElanA.01348.a) | 1 | 503 |
Structures
6B1Z
DEPOSITED: 9/19/2017
DETERMINATION: XRay
CLONE: ElanA.01348.a.B1.GE41090
PROTEIN: ElanA.01348.a.B1.PW38250
Publications by SSGCID
Crystal structures of glutamyl-tRNA synthase from Elizabethkingia anopheles and E. meningosepticum
Dranow DM, Myler PJ, Subramanian S, Mayclin SJ, Asojo OA, Brooks L
Acta Crystallogr F Struct Biol Commun - 2022
volume 78, issue Pt 8, pages 306-312
PMID: 35924598; PMCID: PMC9350836
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|1338011.4.peg.1589 |
| UniProt: | A0A077E909 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKVRVRFAP SPTGPLHLGG VRTALYDYLF AKHNGGDFIL RIEDTDTQRY VPGSEEYIME ALEWIGMVPD ESPKHGGPYA PYRQSERRDI YDRYTEQILK TDYAYLAFDT PEELDQIRAE FEARGDVFAY NYETRNRLRN SISLPEEEVK KLLEEKTPYV IRFKMPLDRI INLNDIIRGK FSVNTNTLDD KVLVKNDGMP TYHFANIIDD HEMKITHVIR GEEWLPSMAL HVLLYEAMGW DAPEFAHLSL ILKPEGKGKL SKRDGDKFGF PVFPLNFTDP ATGNTSAGYR EEGYLPEAFI NMVAMLGWSP ADNKEIVSMD EMIKEFDLNK VHKAGARFSA EKAKWFNQQY LQLMSNEAIL PEFKKVLAEN NVEVSDEKAL KIIGLMKERA TFVKDIYNDG KFFFHAPESF DEKASKKAWS PETAVLMQEL TEAISSLDFK AEIIKESIHH LAEAKGLGMG KVMMPLRLSL VGELKGPDVP DLMEMIGKEE TISRINKAIE TLK
NT Sequence
ATGGAAAAAG TACGGGTACG TTTTGCTCCC AGCCCAACCG GGCCTTTACA TTTGGGAGGT GTACGTACAG CATTATATGA CTATCTGTTT GCAAAACACA ATGGTGGTGA TTTTATCCTG AGAATCGAGG ATACCGATAC TCAGCGTTAT GTTCCGGGTT CCGAAGAGTA CATTATGGAA GCTCTGGAAT GGATTGGTAT GGTTCCGGAT GAAAGTCCAA AACATGGTGG TCCTTATGCT CCATATCGTC AGTCAGAACG TAGAGATATC TATGACAGAT ATACAGAACA AATCCTGAAA ACGGATTATG CTTACCTTGC TTTTGACACA CCTGAAGAAT TGGATCAGAT TCGTGCAGAA TTCGAAGCTA GAGGGGATGT TTTTGCCTAT AACTACGAAA CCCGTAACAG ACTAAGGAAT AGTATTTCTC TTCCTGAAGA AGAAGTAAAA AAACTTCTGG AAGAAAAAAC ACCATATGTA ATCCGTTTCA AAATGCCATT GGACAGAATC ATTAATCTTA ATGATATTAT CCGTGGGAAG TTTAGTGTAA ATACTAATAC CCTGGATGAT AAAGTTTTGG TAAAAAATGA CGGAATGCCA ACCTATCATT TCGCGAACAT TATTGATGAT CACGAGATGA AAATCACTCA TGTTATCCGT GGTGAGGAAT GGCTGCCATC TATGGCACTT CATGTATTAT TATATGAAGC AATGGGTTGG GATGCTCCGG AATTCGCCCA CCTTTCACTT ATCCTGAAAC CTGAAGGAAA AGGTAAACTA AGCAAAAGAG ATGGTGATAA ATTCGGTTTC CCTGTTTTCC CGCTTAACTT TACTGATCCT GCTACAGGCA ATACCTCTGC CGGTTATCGT GAAGAAGGTT ATTTACCAGA AGCTTTCATC AATATGGTTG CCATGTTGGG ATGGTCTCCT GCTGACAATA AAGAAATTGT GAGTATGGAT GAAATGATTA AAGAATTCGA TCTTAATAAA GTTCATAAAG CAGGAGCAAG ATTCAGTGCA GAAAAAGCAA AATGGTTCAA TCAGCAATAT TTGCAACTGA TGTCTAATGA AGCTATACTT CCTGAATTTA AAAAAGTACT GGCAGAAAAC AATGTTGAAG TTTCTGATGA GAAAGCTTTA AAAATCATTG GTCTGATGAA AGAAAGAGCA ACCTTTGTTA AAGACATCTA TAATGACGGT AAATTCTTTT TCCATGCACC GGAATCTTTT GATGAAAAGG CTTCGAAAAA AGCATGGTCT CCGGAAACAG CTGTATTAAT GCAAGAATTA ACCGAGGCTA TTTCATCTCT AGATTTTAAG GCTGAAATTA TTAAAGAAAG TATCCACCAT CTGGCAGAAG CCAAAGGATT GGGTATGGGA AAAGTAATGA TGCCCCTGAG ACTTTCTTTA GTTGGAGAAC TTAAAGGACC AGACGTACCG GATCTTATGG AAATGATCGG AAAAGAAGAG ACTATTTCCA GAATTAATAA AGCAATTGAA ACTTTAAAA
Details for ElanA.01348.a.B1.GE41090
| HARVESTED ON: | 2/8/2017 |
| SEQUENCED ON: | 2/13/2017 |
| EXPECTED MW: | 59kDa |
| OBSERVED MW: | 58kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHME KVRVRFAPSP TGPLHLGGVR TALYDYLFAK HNGGDFILRI EDTDTQRYVP GSEEYIMEAL EWIGMVPDES PKHGGPYAPY RQSERRDIYD RYTEQILKTD YAYLAFDTPE ELDQIRAEFE ARGDVFAYNY ETRNRLRNSI SLPEEEVKKL LEEKTPYVIR FKMPLDRIIN LNDIIRGKFS VNTNTLDDKV LVKNDGMPTY HFANIIDDHE MKITHVIRGE EWLPSMALHV LLYEAMGWDA PEFAHLSLIL KPEGKGKLSK RDGDKFGFPV FPLNFTDPAT GNTSAGYREE GYLPEAFINM VAMLGWSPAD NKEIVSMDEM IKEFDLNKVH KAGARFSAEK AKWFNQQYLQ LMSNEAILPE FKKVLAENNV EVSDEKALKI IGLMKERATF VKDIYNDGKF FFHAPESFDE KASKKAWSPE TAVLMQELTE AISSLDFKAE IIKESIHHLA EAKGLGMGKV MMPLRLSLVG ELKGPDVPDL MEMIGKEETI SRI
Validated NT Sequence
attaattctg gaaatagtct cttcttttcc gatcatttcc ataagatccg gtacgtctgg tcctttaagt tctccaacta aagaaagtct caggggcatc attacttttc ccatacccaa tcctttggct tctgccagat ggtggatact ttctttaata atttcagcct taaaatctag agatgaaata gcctcggtta attcttgcat taatacagct gtttccggag accatgcttt tttcgaagcc ttttcatcaa aagattccgg tgcatggaaa aagaatttac cgtcattata gatgtcttta acaaaggttg ctctttcttt catcagacca atgattttta aagctttctc atcagaaact tcaacattgt tttctgccag tactttttta aattcaggaa gtatagcttc attagacatc agttgcaaat attgctgatt gaaccatttt gctttttctg cactgaatct tgctcctgct ttatgaactt tattaagatc gaattcttta atcatttcat ccatactcac aatttcttta ttgtcagcag gagaccatcc caacatggca accatattga tgaaagcttc tggtaaataa ccttcttcac gataaccggc agaggtattg cctgtagcag gatcagtaaa gttaagcggg aaaacaggga aaccgaattt atcaccatct cttttgctta gtttaccttt tccttcaggt ttcaggataa gtgaaaggtg ggcgaattcc ggagcatccc aacccattgc ttcatataat aatacatgaa gtgccataga tggcagccat tcctcaccac ggataacatg agtgattttc atctcgtgat catcaataat gttcgcgaaa tgataggttg gcattccgtc attttttacc aaaactttat catccagggt attagtattt acactaaact tcccacggat aatatcatta agattaatga ttctgtccaa tggcattttg aaacggatta catatggtgt tttttcttcc agaagttttt ttacttcttc ttcaggaaga gaaatactat tccttagtct gttacgggtt tcgtagttat aggcaaaaac atcccctcta gcttcgaatt ctgcacgaat ctgatccaat tcttcaggtg tgtcaaaagc aaggtaagca taatccgttt tcaggatttg ttctgtatat ctgtcataga tatctctacg ttctgactga cgatatggag cataaggacc accatgtttt ggactttcat ccggaaccat accaatccat tccagagctt ccataatgta ctcttcggaa cccggaacat aacgctgagt atcggtatcc tcgattctca ggataaaatc accaccattg tgttttgcaa acagatagtc atataatgct gtacgtacac ctcccaaatg taaaggcccg gttgggctgg gagcaaaacg tacccgtact ttttccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHME KVRVRFAPSP TGPLHLGGVR TALYDYLFAK HNGGDFILRI EDTDTQRYVP GSEEYIMEAL EWIGMVPDES PKHGGPYAPY RQSERRDIYD RYTEQILKTD YAYLAFDTPE ELDQIRAEFE ARGDVFAYNY ETRNRLRNSI SLPEEEVKKL LEEKTPYVIR FKMPLDRIIN LNDIIRGKFS VNTNTLDDKV LVKNDGMPTY HFANIIDDHE MKITHVIRGE EWLPSMALHV LLYEAMGWDA PEFAHLSLIL KPEGKGKLSK RDGDKFGFPV FPLNFTDPAT GNTSAGYREE GYLPEAFINM VAMLGWSPAD NKEIVSMDEM IKEFDLNKVH KAGARFSAEK AKWFNQQYLQ LMSNEAILPE FKKVLAENNV EVSDEKALKI IGLMKERATF VKDIYNDGKF FFHAPESFDE KASKKAWSPE TAVLMQELTE AISSLDFKAE IIKESIHHLA EAKGLGMGKV MMPLRLSLVG ELKGPDVPDL MEMIGKEETI SRINKAIETL K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa aagtacgggt acgttttgct cccagcccaa ccgggccttt acatttggga ggtgtacgta cagcattata tgactatctg tttgcaaaac acaatggtgg tgattttatc ctgagaatcg aggataccga tactcagcgt tatgttccgg gttccgaaga gtacattatg gaagctctgg aatggattgg tatggttccg gatgaaagtc caaaacatgg tggtccttat gctccatatc gtcagtcaga acgtagagat atctatgaca gatatacaga acaaatcctg aaaacggatt atgcttacct tgcttttgac acacctgaag aattggatca gattcgtgca gaattcgaag ctagagggga tgtttttgcc tataactacg aaacccgtaa cagactaagg aatagtattt ctcttcctga agaagaagta aaaaaacttc tggaagaaaa aacaccatat gtaatccgtt tcaaaatgcc attggacaga atcattaatc ttaatgatat tatccgtggg aagtttagtg taaatactaa taccctggat gataaagttt tggtaaaaaa tgacggaatg ccaacctatc atttcgcgaa cattattgat gatcacgaga tgaaaatcac tcatgttatc cgtggtgagg aatggctgcc atctatggca cttcatgtat tattatatga agcaatgggt tgggatgctc cggaattcgc ccacctttca cttatcctga aacctgaagg aaaaggtaaa ctaagcaaaa gagatggtga taaattcggt ttccctgttt tcccgcttaa ctttactgat cctgctacag gcaatacctc tgccggttat cgtgaagaag gttatttacc agaagctttc atcaatatgg ttgccatgtt gggatggtct cctgctgaca ataaagaaat tgtgagtatg gatgaaatga ttaaagaatt cgatcttaat aaagttcata aagcaggagc aagattcagt gcagaaaaag caaaatggtt caatcagcaa tatttgcaac tgatgtctaa tgaagctata cttcctgaat ttaaaaaagt actggcagaa aacaatgttg aagtttctga tgagaaagct ttaaaaatca ttggtctgat gaaagaaaga gcaacctttg ttaaagacat ctataatgac ggtaaattct ttttccatgc accggaatct tttgatgaaa aggcttcgaa aaaagcatgg tctccggaaa cagctgtatt aatgcaagaa ttaaccgagg ctatttcatc tctagatttt aaggctgaaa ttattaaaga aagtatccac catctggcag aagccaaagg attgggtatg ggaaaagtaa tgatgcccct gagactttct ttagttggag aacttaaagg accagacgta ccggatctta tggaaatgat cggaaaagaa gagactattt ccagaattaa taaagcaatt gaaactttaa aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for ElanA.01348.a.B1.PW38250
| PURIFICATION DATe: | 4/10/2017 |
| CONCENTRATION: | 36.5mg/ml |
| OBSERVED MW: | 61kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 13 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME KVRVRFAPSP TGPLHLGGVR TALYDYLFAK HNGGDFILRI EDTDTQRYVP GSEEYIMEAL EWIGMVPDES PKHGGPYAPY RQSERRDIYD RYTEQILKTD YAYLAFDTPE ELDQIRAEFE ARGDVFAYNY ETRNRLRNSI SLPEEEVKKL LEEKTPYVIR FKMPLDRIIN LNDIIRGKFS VNTNTLDDKV LVKNDGMPTY HFANIIDDHE MKITHVIRGE EWLPSMALHV LLYEAMGWDA PEFAHLSLIL KPEGKGKLSK RDGDKFGFPV FPLNFTDPAT GNTSAGYREE GYLPEAFINM VAMLGWSPAD NKEIVSMDEM IKEFDLNKVH KAGARFSAEK AKWFNQQYLQ LMSNEAILPE FKKVLAENNV EVSDEKALKI IGLMKERATF VKDIYNDGKF FFHAPESFDE KASKKAWSPE TAVLMQELTE AISSLDFKAE IIKESIHHLA EAKGLGMGKV MMPLRLSLVG ELKGPDVPDL MEMIGKEETI SRI
Validated NT Sequence
attaattctg gaaatagtct cttcttttcc gatcatttcc ataagatccg gtacgtctgg tcctttaagt tctccaacta aagaaagtct caggggcatc attacttttc ccatacccaa tcctttggct tctgccagat ggtggatact ttctttaata atttcagcct taaaatctag agatgaaata gcctcggtta attcttgcat taatacagct gtttccggag accatgcttt tttcgaagcc ttttcatcaa aagattccgg tgcatggaaa aagaatttac cgtcattata gatgtcttta acaaaggttg ctctttcttt catcagacca atgattttta aagctttctc atcagaaact tcaacattgt tttctgccag tactttttta aattcaggaa gtatagcttc attagacatc agttgcaaat attgctgatt gaaccatttt gctttttctg cactgaatct tgctcctgct ttatgaactt tattaagatc gaattcttta atcatttcat ccatactcac aatttcttta ttgtcagcag gagaccatcc caacatggca accatattga tgaaagcttc tggtaaataa ccttcttcac gataaccggc agaggtattg cctgtagcag gatcagtaaa gttaagcggg aaaacaggga aaccgaattt atcaccatct cttttgctta gtttaccttt tccttcaggt ttcaggataa gtgaaaggtg ggcgaattcc ggagcatccc aacccattgc ttcatataat aatacatgaa gtgccataga tggcagccat tcctcaccac ggataacatg agtgattttc atctcgtgat catcaataat gttcgcgaaa tgataggttg gcattccgtc attttttacc aaaactttat catccagggt attagtattt acactaaact tcccacggat aatatcatta agattaatga ttctgtccaa tggcattttg aaacggatta catatggtgt tttttcttcc agaagttttt ttacttcttc ttcaggaaga gaaatactat tccttagtct gttacgggtt tcgtagttat aggcaaaaac atcccctcta gcttcgaatt ctgcacgaat ctgatccaat tcttcaggtg tgtcaaaagc aaggtaagca taatccgttt tcaggatttg ttctgtatat ctgtcataga tatctctacg ttctgactga cgatatggag cataaggacc accatgtttt ggactttcat ccggaaccat accaatccat tccagagctt ccataatgta ctcttcggaa cccggaacat aacgctgagt atcggtatcc tcgattctca ggataaaatc accaccattg tgttttgcaa acagatagtc atataatgct gtacgtacac ctcccaaatg taaaggcccg gttgggctgg gagcaaaacg tacccgtact ttttccatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHME KVRVRFAPSP TGPLHLGGVR TALYDYLFAK HNGGDFILRI EDTDTQRYVP GSEEYIMEAL EWIGMVPDES PKHGGPYAPY RQSERRDIYD RYTEQILKTD YAYLAFDTPE ELDQIRAEFE ARGDVFAYNY ETRNRLRNSI SLPEEEVKKL LEEKTPYVIR FKMPLDRIIN LNDIIRGKFS VNTNTLDDKV LVKNDGMPTY HFANIIDDHE MKITHVIRGE EWLPSMALHV LLYEAMGWDA PEFAHLSLIL KPEGKGKLSK RDGDKFGFPV FPLNFTDPAT GNTSAGYREE GYLPEAFINM VAMLGWSPAD NKEIVSMDEM IKEFDLNKVH KAGARFSAEK AKWFNQQYLQ LMSNEAILPE FKKVLAENNV EVSDEKALKI IGLMKERATF VKDIYNDGKF FFHAPESFDE KASKKAWSPE TAVLMQELTE AISSLDFKAE IIKESIHHLA EAKGLGMGKV MMPLRLSLVG ELKGPDVPDL MEMIGKEETI SRINKAIETL K
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAA AAGTACGGGT ACGTTTTGCT CCCAGCCCAA CCGGGCCTTT ACATTTGGGA GGTGTACGTA CAGCATTATA TGACTATCTG TTTGCAAAAC ACAATGGTGG TGATTTTATC CTGAGAATCG AGGATACCGA TACTCAGCGT TATGTTCCGG GTTCCGAAGA GTACATTATG GAAGCTCTGG AATGGATTGG TATGGTTCCG GATGAAAGTC CAAAACATGG TGGTCCTTAT GCTCCATATC GTCAGTCAGA ACGTAGAGAT ATCTATGACA GATATACAGA ACAAATCCTG AAAACGGATT ATGCTTACCT TGCTTTTGAC ACACCTGAAG AATTGGATCA GATTCGTGCA GAATTCGAAG CTAGAGGGGA TGTTTTTGCC TATAACTACG AAACCCGTAA CAGACTAAGG AATAGTATTT CTCTTCCTGA AGAAGAAGTA AAAAAACTTC TGGAAGAAAA AACACCATAT GTAATCCGTT TCAAAATGCC ATTGGACAGA ATCATTAATC TTAATGATAT TATCCGTGGG AAGTTTAGTG TAAATACTAA TACCCTGGAT GATAAAGTTT TGGTAAAAAA TGACGGAATG CCAACCTATC ATTTCGCGAA CATTATTGAT GATCACGAGA TGAAAATCAC TCATGTTATC CGTGGTGAGG AATGGCTGCC ATCTATGGCA CTTCATGTAT TATTATATGA AGCAATGGGT TGGGATGCTC CGGAATTCGC CCACCTTTCA CTTATCCTGA AACCTGAAGG AAAAGGTAAA CTAAGCAAAA GAGATGGTGA TAAATTCGGT TTCCCTGTTT TCCCGCTTAA CTTTACTGAT CCTGCTACAG GCAATACCTC TGCCGGTTAT CGTGAAGAAG GTTATTTACC AGAAGCTTTC ATCAATATGG TTGCCATGTT GGGATGGTCT CCTGCTGACA ATAAAGAAAT TGTGAGTATG GATGAAATGA TTAAAGAATT CGATCTTAAT AAAGTTCATA AAGCAGGAGC AAGATTCAGT GCAGAAAAAG CAAAATGGTT CAATCAGCAA TATTTGCAAC TGATGTCTAA TGAAGCTATA CTTCCTGAAT TTAAAAAAGT ACTGGCAGAA AACAATGTTG AAGTTTCTGA TGAGAAAGCT TTAAAAATCA TTGGTCTGAT GAAAGAAAGA GCAACCTTTG TTAAAGACAT CTATAATGAC GGTAAATTCT TTTTCCATGC ACCGGAATCT TTTGATGAAA AGGCTTCGAA AAAAGCATGG TCTCCGGAAA CAGCTGTATT AATGCAAGAA TTAACCGAGG CTATTTCATC TCTAGATTTT AAGGCTGAAA TTATTAAAGA AAGTATCCAC CATCTGGCAG AAGCCAAAGG ATTGGGTATG GGAAAAGTAA TGATGCCCCT GAGACTTTCT TTAGTTGGAG AACTTAAAGG ACCAGACGTA CCGGATCTTA TGGAAATGAT CGGAAAAGAA GAGACTATTT CCAGAATTAA TAAAGCAATT GAAACTTTAA AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT