ElanA.01322.a

Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A

CENTER ID: ElanA.01322.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.01322.a.B1.GE40655 Full length(ElanA.01322.a) 1 232
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.01322.a.B1.PW38279 Full length(ElanA.01322.a) 1 232
ElanA.01322.a.B1.PW38280 Full length(ElanA.01322.a) 1 232

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.901
UniProt: A0A077EK03

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MINKTVKNVQ EACEGIQDGM TIMLGGFGLC GIPENCITEL VKKEIKDLTC ISNNAGVDNF GLGLLLHKRQ IKKMISSYVG ENAEFERQLL SGELEVELIP QGTLATRCMA AGYGMPAVYT PAGVGTEVAE GKEVRNFNGK DYLLEYAFDA DFAIVKAWKG DTAGNLIFRS TSRNFNPVMA MAGKITIAEV EELVEVGELN PDEIHTPGIY VHRIFQGDHY EKRIEQRTVR KK
NT Sequence
ATGATTAATA AAACTGTTAA AAATGTACAG GAGGCCTGCG AAGGGATTCA GGATGGCATG ACAATCATGC TTGGAGGTTT CGGTCTTTGC GGAATTCCTG AAAATTGTAT TACAGAATTG GTAAAAAAGG AAATAAAAGA CCTTACCTGT ATTTCTAATA ATGCTGGAGT CGATAATTTT GGACTTGGCT TACTTCTTCA TAAAAGACAA ATCAAGAAAA TGATATCTTC TTATGTTGGT GAAAATGCGG AATTTGAACG CCAGCTACTT AGTGGTGAAC TTGAAGTGGA ACTGATCCCA CAAGGGACAT TAGCTACACG TTGTATGGCT GCAGGCTACG GAATGCCAGC TGTCTATACC CCTGCAGGTG TAGGAACTGA GGTTGCGGAG GGTAAAGAGG TACGTAATTT TAATGGTAAA GACTATTTGT TGGAATATGC TTTTGATGCT GATTTTGCAA TTGTAAAGGC ATGGAAAGGC GATACTGCTG GAAATCTTAT TTTCCGTTCT ACTTCCAGAA ACTTTAATCC GGTAATGGCT ATGGCTGGTA AAATAACAAT TGCTGAAGTT GAAGAATTGG TTGAAGTTGG CGAGCTTAAT CCCGATGAAA TCCATACTCC CGGAATCTAT GTTCATAGAA TATTCCAGGG AGATCATTAT GAAAAAAGAA TCGAACAGAG AACGGTAAGA AAAAAA
Details for ElanA.01322.a.B1.GE40655
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI NKTVKNVQEA CEGIQDGMTI MLGGFGLCGI PENCITELVK KEIKDLTCIS NNAGVDNFGL GLLLHKRQIK KMISSYVGEN AEFERQLLSG ELEVELIPQG TLATRCMAAG YGMPAVYTPA GVGTEVAEGK EVRNFNGKDY LLEYAFDADF AIVKAWKGDT AGNLIFRSTS RNFNPVMAMA GKITIAEVEE LVEVGELNPD EIHTPGIYVH RIFQGDHYEK RIEQRTVRKK
Validated NT Sequence
atggctcacc accaccacca ccatatgatt aataaaactg ttaaaaatgt acaggaggcc tgcgaaggga ttcaggatgg catgacaatc atgcttggag gtttcggtct ttgcggaatt cctgaaaatt gtattacaga attggtaaaa aaggaaataa aagaccttac ctgtatttct aataatgctg gagtcgataa ttttggactt ggcttacttc ttcataaaag acaaatcaag aaaatgatat cttcttatgt tggtgaaaat gcggaatttg aacgccagct acttagtggt gaacttgaag tggaactgat cccacaaggg acattagcta cacgttgtat ggctgcaggc tacggaatgc cagctgtcta tacccctgca ggtgtaggaa ctgaggttgc ggagggtaaa gaggtacgta attttaatgg taaagactat ttgttggaat atgcttttga tgctgatttt gcaattgtaa aggcatggaa aggcgatact gctggaaatc ttattttccg ttctacttcc agaaacttta atccggtaat ggctatggct ggtaaaataa caattgctga agttgaagaa ttggttgaag ttggcgagct taatcccgat gaaatccata ctcccggaat ctatgttcat agaatattcc agggagatca ttatgaaaaa agaatcgaac agagaacggt aagaaaaaaa taagtgagta agat
Expected Protein Sequence
MAHHHHHHMI NKTVKNVQEA CEGIQDGMTI MLGGFGLCGI PENCITELVK KEIKDLTCIS NNAGVDNFGL GLLLHKRQIK KMISSYVGEN AEFERQLLSG ELEVELIPQG TLATRCMAAG YGMPAVYTPA GVGTEVAEGK EVRNFNGKDY LLEYAFDADF AIVKAWKGDT AGNLIFRSTS RNFNPVMAMA GKITIAEVEE LVEVGELNPD EIHTPGIYVH RIFQGDHYEK RIEQRTVRKK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatta ataaaactgt taaaaatgta caggaggcct gcgaagggat tcaggatggc atgacaatca tgcttggagg tttcggtctt tgcggaattc ctgaaaattg tattacagaa ttggtaaaaa aggaaataaa agaccttacc tgtatttcta ataatgctgg agtcgataat tttggacttg gcttacttct tcataaaaga caaatcaaga aaatgatatc ttcttatgtt ggtgaaaatg cggaatttga acgccagcta cttagtggtg aacttgaagt ggaactgatc ccacaaggga cattagctac acgttgtatg gctgcaggct acggaatgcc agctgtctat acccctgcag gtgtaggaac tgaggttgcg gagggtaaag aggtacgtaa ttttaatggt aaagactatt tgttggaata tgcttttgat gctgattttg caattgtaaa ggcatggaaa ggcgatactg ctggaaatct tattttccgt tctacttcca gaaactttaa tccggtaatg gctatggctg gtaaaataac aattgctgaa gttgaagaat tggttgaagt tggcgagctt aatcccgatg aaatccatac tcccggaatc tatgttcata gaatattcca gggagatcat tatgaaaaaa gaatcgaaca gagaacggta agaaaaaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for ElanA.01322.a.B1.PW38279
PURIFICATION DATe: 5/17/2017
CONCENTRATION: 16mg/ml
OBSERVED MW: 27kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI NKTVKNVQEA CEGIQDGMTI MLGGFGLCGI PENCITELVK KEIKDLTCIS NNAGVDNFGL GLLLHKRQIK KMISSYVGEN AEFERQLLSG ELEVELIPQG TLATRCMAAG YGMPAVYTPA GVGTEVAEGK EVRNFNGKDY LLEYAFDADF AIVKAWKGDT AGNLIFRSTS RNFNPVMAMA GKITIAEVEE LVEVGELNPD EIHTPGIYVH RIFQGDHYEK RIEQRTVRKK
Validated NT Sequence
atggctcacc accaccacca ccatatgatt aataaaactg ttaaaaatgt acaggaggcc tgcgaaggga ttcaggatgg catgacaatc atgcttggag gtttcggtct ttgcggaatt cctgaaaatt gtattacaga attggtaaaa aaggaaataa aagaccttac ctgtatttct aataatgctg gagtcgataa ttttggactt ggcttacttc ttcataaaag acaaatcaag aaaatgatat cttcttatgt tggtgaaaat gcggaatttg aacgccagct acttagtggt gaacttgaag tggaactgat cccacaaggg acattagcta cacgttgtat ggctgcaggc tacggaatgc cagctgtcta tacccctgca ggtgtaggaa ctgaggttgc ggagggtaaa gaggtacgta attttaatgg taaagactat ttgttggaat atgcttttga tgctgatttt gcaattgtaa aggcatggaa aggcgatact gctggaaatc ttattttccg ttctacttcc agaaacttta atccggtaat ggctatggct ggtaaaataa caattgctga agttgaagaa ttggttgaag ttggcgagct taatcccgat gaaatccata ctcccggaat ctatgttcat agaatattcc agggagatca ttatgaaaaa agaatcgaac agagaacggt aagaaaaaaa taagtgagta agat
Expressed Protein Sequence
MAHHHHHHMI NKTVKNVQEA CEGIQDGMTI MLGGFGLCGI PENCITELVK KEIKDLTCIS NNAGVDNFGL GLLLHKRQIK KMISSYVGEN AEFERQLLSG ELEVELIPQG TLATRCMAAG YGMPAVYTPA GVGTEVAEGK EVRNFNGKDY LLEYAFDADF AIVKAWKGDT AGNLIFRSTS RNFNPVMAMA GKITIAEVEE LVEVGELNPD EIHTPGIYVH RIFQGDHYEK RIEQRTVRKK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATTA ATAAAACTGT TAAAAATGTA CAGGAGGCCT GCGAAGGGAT TCAGGATGGC ATGACAATCA TGCTTGGAGG TTTCGGTCTT TGCGGAATTC CTGAAAATTG TATTACAGAA TTGGTAAAAA AGGAAATAAA AGACCTTACC TGTATTTCTA ATAATGCTGG AGTCGATAAT TTTGGACTTG GCTTACTTCT TCATAAAAGA CAAATCAAGA AAATGATATC TTCTTATGTT GGTGAAAATG CGGAATTTGA ACGCCAGCTA CTTAGTGGTG AACTTGAAGT GGAACTGATC CCACAAGGGA CATTAGCTAC ACGTTGTATG GCTGCAGGCT ACGGAATGCC AGCTGTCTAT ACCCCTGCAG GTGTAGGAAC TGAGGTTGCG GAGGGTAAAG AGGTACGTAA TTTTAATGGT AAAGACTATT TGTTGGAATA TGCTTTTGAT GCTGATTTTG CAATTGTAAA GGCATGGAAA GGCGATACTG CTGGAAATCT TATTTTCCGT TCTACTTCCA GAAACTTTAA TCCGGTAATG GCTATGGCTG GTAAAATAAC AATTGCTGAA GTTGAAGAAT TGGTTGAAGT TGGCGAGCTT AATCCCGATG AAATCCATAC TCCCGGAATC TATGTTCATA GAATATTCCA GGGAGATCAT TATGAAAAAA GAATCGAACA GAGAACGGTA AGAAAAAAAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT
Details for ElanA.01322.a.B1.PW38280
PURIFICATION DATe: 5/17/2017
CONCENTRATION: 32mg/ml
OBSERVED MW: 27kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI NKTVKNVQEA CEGIQDGMTI MLGGFGLCGI PENCITELVK KEIKDLTCIS NNAGVDNFGL GLLLHKRQIK KMISSYVGEN AEFERQLLSG ELEVELIPQG TLATRCMAAG YGMPAVYTPA GVGTEVAEGK EVRNFNGKDY LLEYAFDADF AIVKAWKGDT AGNLIFRSTS RNFNPVMAMA GKITIAEVEE LVEVGELNPD EIHTPGIYVH RIFQGDHYEK RIEQRTVRKK
Validated NT Sequence
atggctcacc accaccacca ccatatgatt aataaaactg ttaaaaatgt acaggaggcc tgcgaaggga ttcaggatgg catgacaatc atgcttggag gtttcggtct ttgcggaatt cctgaaaatt gtattacaga attggtaaaa aaggaaataa aagaccttac ctgtatttct aataatgctg gagtcgataa ttttggactt ggcttacttc ttcataaaag acaaatcaag aaaatgatat cttcttatgt tggtgaaaat gcggaatttg aacgccagct acttagtggt gaacttgaag tggaactgat cccacaaggg acattagcta cacgttgtat ggctgcaggc tacggaatgc cagctgtcta tacccctgca ggtgtaggaa ctgaggttgc ggagggtaaa gaggtacgta attttaatgg taaagactat ttgttggaat atgcttttga tgctgatttt gcaattgtaa aggcatggaa aggcgatact gctggaaatc ttattttccg ttctacttcc agaaacttta atccggtaat ggctatggct ggtaaaataa caattgctga agttgaagaa ttggttgaag ttggcgagct taatcccgat gaaatccata ctcccggaat ctatgttcat agaatattcc agggagatca ttatgaaaaa agaatcgaac agagaacggt aagaaaaaaa taagtgagta agat
Expressed Protein Sequence
MAHHHHHHMI NKTVKNVQEA CEGIQDGMTI MLGGFGLCGI PENCITELVK KEIKDLTCIS NNAGVDNFGL GLLLHKRQIK KMISSYVGEN AEFERQLLSG ELEVELIPQG TLATRCMAAG YGMPAVYTPA GVGTEVAEGK EVRNFNGKDY LLEYAFDADF AIVKAWKGDT AGNLIFRSTS RNFNPVMAMA GKITIAEVEE LVEVGELNPD EIHTPGIYVH RIFQGDHYEK RIEQRTVRKK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATTA ATAAAACTGT TAAAAATGTA CAGGAGGCCT GCGAAGGGAT TCAGGATGGC ATGACAATCA TGCTTGGAGG TTTCGGTCTT TGCGGAATTC CTGAAAATTG TATTACAGAA TTGGTAAAAA AGGAAATAAA AGACCTTACC TGTATTTCTA ATAATGCTGG AGTCGATAAT TTTGGACTTG GCTTACTTCT TCATAAAAGA CAAATCAAGA AAATGATATC TTCTTATGTT GGTGAAAATG CGGAATTTGA ACGCCAGCTA CTTAGTGGTG AACTTGAAGT GGAACTGATC CCACAAGGGA CATTAGCTAC ACGTTGTATG GCTGCAGGCT ACGGAATGCC AGCTGTCTAT ACCCCTGCAG GTGTAGGAAC TGAGGTTGCG GAGGGTAAAG AGGTACGTAA TTTTAATGGT AAAGACTATT TGTTGGAATA TGCTTTTGAT GCTGATTTTG CAATTGTAAA GGCATGGAAA GGCGATACTG CTGGAAATCT TATTTTCCGT TCTACTTCCA GAAACTTTAA TCCGGTAATG GCTATGGCTG GTAAAATAAC AATTGCTGAA GTTGAAGAAT TGGTTGAAGT TGGCGAGCTT AATCCCGATG AAATCCATAC TCCCGGAATC TATGTTCATA GAATATTCCA GGGAGATCAT TATGAAAAAA GAATCGAACA GAGAACGGTA AGAAAAAAAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT