ElanA.01317.a

ATP-dependent Clp protease proteolytic subunit

CENTER ID: ElanA.01317.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.01317.a.B1.GE40654 Full length(ElanA.01317.a) 1 227
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.2179
UniProt: A0A077EBU2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDIKKEFRDY AVKHLGVNGL AADQYMGIYG PTNLTPYIME ERRLNVAQMD VFSRLMMDRI IFLGTGIDDQ VANIVTAQLL FLESADPAKD IQIYINSPGG SVYAGLGIYD TMQIIKPDVA TICTGMAASM GAVLLVAGEK GKRSALKHSR VMIHQPSGGA QGVASDMEIN LREMLKLKTE LYDIIAHHSG QTYEWVEKSS DRDYWMTSEE AKSFGMVDEV LQRKEEK
NT Sequence
ATGGATATTA AAAAAGAATT TAGAGATTAC GCAGTAAAAC ACCTTGGAGT GAATGGTCTT GCTGCTGATC AGTATATGGG GATATATGGT CCAACTAATT TAACGCCATA CATTATGGAG GAGCGTCGTC TAAATGTAGC GCAAATGGAT GTTTTTTCCC GTTTGATGAT GGACAGAATT ATCTTTCTTG GGACAGGGAT CGATGATCAG GTTGCCAACA TTGTAACGGC TCAGTTATTA TTCCTGGAAA GTGCTGATCC TGCTAAAGAT ATTCAGATCT ATATCAACTC TCCTGGTGGT AGTGTATATG CCGGATTAGG AATCTATGAT ACCATGCAGA TTATTAAACC TGATGTAGCT ACGATCTGTA CAGGTATGGC AGCATCAATG GGTGCTGTAT TATTGGTTGC AGGAGAAAAA GGTAAACGTT CTGCTTTGAA GCATTCAAGA GTGATGATTC ACCAGCCAAG TGGGGGTGCT CAGGGTGTAG CTTCCGATAT GGAAATCAAC CTTCGTGAAA TGTTGAAATT AAAAACAGAA TTATACGATA TCATTGCTCA CCACTCAGGT CAGACTTATG AATGGGTAGA GAAATCTTCC GACAGAGATT ACTGGATGAC TTCTGAAGAA GCTAAGAGCT TCGGTATGGT AGACGAGGTA CTACAGCGTA AAGAAGAGAA A
Details for ElanA.01317.a.B1.GE40654
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHMGIK KEFRDYAVKH LGVNGLAADQ YMGIYGPTNL TPYIMEERRL NVAQMDVFSR LMMDRIIFLG TGIDDQVANI VTAQLLFLES ADPAKDIQIY INSPGGSVYA GLGIYDTMQI IKPDVATICT GMAASMGAVL LVAGEKGKRS ALKHSRVMIH QPSGGAQGVA SDMEINLREM LKLKTELYDI IAHHSGQTYE WVEKSSDRDY WMTSEEAKSF GMVDEVLQRK EEK
Validated NT Sequence
atggctcacc accaccatat gggtattaaa aaagaattta gagattacgc agtaaaacac cttggagtga atggtcttgc tgctgatcag tatatgggga tatatggtcc aactaattta acgccataca ttatggagga gcgtcgtcta aatgtagcgc aaatggatgt tttttcccgt ttgatgatgg acagaattat ctttcttggg acagggatcg atgatcaggt tgccaacatt gtaacggctc agttattatt cctggaaagt gctgatcctg ctaaagatat tcagatctat atcaactctc ctggtggtag tgtatatgcc ggattaggaa tctatgatac catgcagatt attaaacctg atgtagctac gatctgtaca ggtatggcag catcaatggg tgctgtatta ttggttgcag gagaaaaagg taaacgttct gctttgaagc attcaagagt gatgattcac cagccaagtg ggggtgctca gggtgtagct tccgatatgg aaatcaacct tcgtgaaatg ttgaaattaa aaacagaatt atacgatatc attgctcacc actcaggtca gacttatgaa tgggtagaga aatcttccga cagagattac tggatgactt ctgaagaagc taagagcttc ggtatggtag acgaggtact acagcgtaaa gaagagaaat aagtgagtaa gat
Expected Protein Sequence
MAHHHHHHMD IKKEFRDYAV KHLGVNGLAA DQYMGIYGPT NLTPYIMEER RLNVAQMDVF SRLMMDRIIF LGTGIDDQVA NIVTAQLLFL ESADPAKDIQ IYINSPGGSV YAGLGIYDTM QIIKPDVATI CTGMAASMGA VLLVAGEKGK RSALKHSRVM IHQPSGGAQG VASDMEINLR EMLKLKTELY DIIAHHSGQT YEWVEKSSDR DYWMTSEEAK SFGMVDEVLQ RKEEK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggata ttaaaaaaga atttagagat tacgcagtaa aacaccttgg agtgaatggt cttgctgctg atcagtatat ggggatatat ggtccaacta atttaacgcc atacattatg gaggagcgtc gtctaaatgt agcgcaaatg gatgtttttt cccgtttgat gatggacaga attatctttc ttgggacagg gatcgatgat caggttgcca acattgtaac ggctcagtta ttattcctgg aaagtgctga tcctgctaaa gatattcaga tctatatcaa ctctcctggt ggtagtgtat atgccggatt aggaatctat gataccatgc agattattaa acctgatgta gctacgatct gtacaggtat ggcagcatca atgggtgctg tattattggt tgcaggagaa aaaggtaaac gttctgcttt gaagcattca agagtgatga ttcaccagcc aagtgggggt gctcagggtg tagcttccga tatggaaatc aaccttcgtg aaatgttgaa attaaaaaca gaattatacg atatcattgc tcaccactca ggtcagactt atgaatgggt agagaaatct tccgacagag attactggat gacttctgaa gaagctaaga gcttcggtat ggtagacgag gtactacagc gtaaagaaga gaaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat